Entrepreneurship skill is considered as an essential skill in the current era of Industry 4.0. The past literature has reported that entrepreneur intention of an individual plays a significant role in his or her decision to establish a new firm and getting involved with business activities. Hence, this study aims to investigate the influence of the personality and self-efficacy on entrepreneurial intention among the future technical professionals in the non-for-profit higher education institution of Malaysia. Moreover, the moderating effect of gender on the relationship between personality and self-efficacy on entrepreneurial intention was also examined in the present study. A total of 475 responses were collected with the use of survey questionnaire from the future technical professionals from non-for-profit higher education institutions in Malaysia. The results of this study show that personality and attitude of future technical professionals were found to have a significant impact on the entrepreneurial intention. Furthermore, gender also found to have a moderating effect on the relationship between personality and self-efficacy on entrepreneurial intention. The practical implications of the research findings were discussed.