scholarly journals Comparative evaluation of anti-bacterial efficacy of silver nanoparticle, nanoparticle calcium hydroxide and calcium hydroxide against enterococcus faecalis biofilm- An In vitro study

2018 ◽  
Vol 6 (2) ◽  
pp. 57
Author(s):  
Dr.Abirami V. ◽  
Dr.A.Shafie Ahamed ◽  
Dr.Bhavani S. ◽  
Dr.Rajaraman G.
Keyword(s):  
Silver Nanoparticles ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Silver Nanoparticle ◽  
In Vitro Study ◽  
P Value ◽  
Antibacterial Efficacy ◽  
Significant Difference ◽  
Post Hoc

The purpose of this study was to evaluate the antibacterial efficacy of silver Nanoparticles, Nanoparticle calcium hydroxide and calcium hydroxide as intracanal medicament against Enterococcus faecalis bio film formed on root dentinThe Minimal inhibitory concentration test and Anti microbial test were conducted for silver nanoparticle, Nanoparticle calcium hydroxide. Teeth were inoculated with E.faecalis strains for 1month to establish a standard mono species bio film model. Biofilm formation is confirmed using SEM. The intracanal medicaments were placed inside the canal and colony forming units (CFU) were counted before and 7 days later the placement of the medicament.MIC for AGNP is 25µg/200µl and for NCH is 12.5µg/200µl. The Antimicrobial test conducted shows zone of inhibition about 11mm and 14mm for AGNP and NCH respectively at 500µg. The calcium hydroxides do not show any antibacterial activity at this concentration. In antibacterial efficacy test using CFU count, the AGNP and NCH shows 75% and 61% of antibacterial efficacy respectively whereas calcium hydroxide has exhibited just 11% of antibacterial efficacy. Silver Nanoparticles and nanoparticle calcium hydroxide had statistically significant difference in the reduction in the number of colonies when compared to calcium hydroxide by post hoc turkey test and p-value of <0.01 was considered as significant in this study.

scholarly journals Antibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro study

2018 ◽  
Vol 9 (2) ◽  
pp. 237 ◽  
Author(s):  
KiranRahul Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Vandana Rathod ◽  
Rahul Halkai
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

scholarly journals Evaluation of the Antibacterial Efficacy of Daptomycin, Gentamicin, and Calcium Hydroxide—Antibiotic Combinations on Enterococcus faecalis Dentinal Biofilm: An In Vitro Study

2021 ◽  
Vol 22 (2) ◽  
pp. 128-133
Author(s):  
Arunajatesan Subbiya ◽  
Suresh Mitthra ◽  
Kesavaram Padmavathy ◽  
Krishnan Mahalakshmi ◽  
Alagarsamy Venkatesh ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

Comparison of the Antibacterial Efficacy of Silver Nanoparticles with Chlorhexidine Against Enterococcus faecalis Biofilm—An In Vitro Study

2017 ◽  
Vol 17 (7) ◽  
pp. 4613-4617 ◽  
Author(s):  
Venkateshbabu Nagendrababu ◽  
Gowri Meiyazhagan ◽  
Winfred Sofi Beaula ◽  
Rajeswari Kalaiselvam ◽  
Balakumar Subramanian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy

scholarly journals Antimicrobial Efficacy of Calcium Hydroxide and Triple Antibiotic Paste Combination on E. faecalis Biofilm- an In vitro Study

2020 ◽  
pp. 120-126
Author(s):  
Azima Hanin S. M. ◽  
Anjaneyulu K. ◽  
Muralidharan N. P.
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
P Value ◽  
Tryptone Soya Agar ◽  
Triple Antibiotic Paste ◽  
High Alkalinity ◽  
Post Hoc

Calcium hydroxide (CH) is an intracanal medicament that has been widely used in endodontics, which can eliminate bacteria because of its high alkalinity. However, E. faecalis is resistant to CH. Triple antibiotic paste (TAP) is a mixture of ciprofloxacin, minocycline, and metronidazole, and is highly effective against E. faecalis. Hence the main aim of this study was to find the antimicrobial efficacy of CH and TAP combination against E. faecalis. The study was done by agar diffusion method, three wells were punched in Tryptone soya agar and filled with CH, TAP, and the combination of both. The zone of inhibition values was recorded and subjected to statistical analysis using SPSS. One way ANOVA and Post Hoc tests were used to compare the means. The combination of CH with TAP was found to be significantly better than CH and TAP used alone (p-value <0.05).Within the limits of this study, it can be concluded that TAP is more efficient when compared with CH,The combination of CH and TAP proved to be more effective when compared to the two when used alone.

scholarly journals ACTIVIDAD ANTIBACTERIANA DE NANOPARTÍCULAS DE PLATA INMOVILIZADAS EN CEMENTO DE ÓXIDO DE ZINC-EUGENOL CONTRA EL ENTEROCOCCUS FAECALIS: ESTUDIO IN VITRO

2019 ◽  
Vol 30 (2) ◽  
Author(s):  
Juan Camilo Duque-Aristizabal ◽  
Liza Maria Isaza-Areiza ◽  
Diego Tobon-Calle ◽  
Martha Elena Londoño Lopez
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
The Other ◽  
Future Research ◽  
Visible Spectroscopy ◽  
Starting Point ◽  
Significant Difference ◽  
Uv Visible

Introduction: the aim of this study is to evaluate the antimicrobial capacity of silver nanoparticles immobilized in a zinc oxide-eugenol (ZOE) cement against Enterococcus faecalis for potential use in endodontic treatments. Method: experimental in vitro study, performing synthesis of silver nanoparticles (AgNPs) and UV-visible spectroscopy to confirm presence of AgNPs in the prepared solutions. The ZOE mixture was standardized, producing the integrated AgNPs/ZOE material. Scanning electron microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) were used to characterize the integrated material; a KirbyBauer assay was finally run to measure the inhibition halos produced by the compound-microorganism interaction. Results: the UV-visible spectroscopy showed presence of AgNPs in the created solution; both SEM and FTIR show that the AgNPs are integrated into the ZOE system, not altering their properties when performed under conditions like those found in the mouth. The Kirby-Bauer assay shows that all samples had inhibition halos. The AgNPs in guava extract had statistically significant differences with the halos of the other samples (p < 0.05). Conclusions: the obtained AgNPs show bactericidal activity against Enterococcus faecalis, as a statistically significant difference was found between the AgNPs suspended in guava extract and the other groups; this will be the starting point for future research

An In Vitro Study Comparing the Intracanal Effectiveness of Calcium Hydroxide– and Linezolid-based Medicaments against Enterococcus faecalis

2012 ◽  
Vol 38 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Rajdeep Pavaskar ◽  
Ida de Noronha de Ataide ◽  
Paul Chalakkal ◽  
Maria J. Pinto ◽  
Kristlee Sabrin Fernandes ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

2020 ◽  
Vol 11 (2) ◽  
pp. 160-166
Author(s):  
Mohammad Javad Moghaddas ◽  
Horieh Moosavi ◽  
Sara Yaghoubirad ◽  
Nasim Chiniforush
Keyword(s):  
Bioactive Glass ◽  
In Vitro Study ◽  
Control Group ◽  
Dentinal Tubule ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Post Hoc ◽  
Caries Lesions ◽  
Cavity Floor

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

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