The Effect of the Bioactive Glass and the Er:YAG Laser on the Remineralization of the Affected Dentin: A Comparative In Vitro Study

Introduction: The purpose of this study was to compare the effect of the bioactive glass, the glass ionomer, and the Erbium YAG laser as liners on the remineralization of the affected dentin. Methods: The present study was conducted on 64 healthy extracted human molars divided into 4 groups, 1 control group and 3 experimental groups. After artificially inducing dentinal caries lesions, each of the experimental groups was applied to the cavity floor and then restored with a composite. The samples were stored after thermocycling in an incubator for two months. Finally, the hardness of the cavity floor was measured at 3 depths of 20, 50 and 100 μm by the Vickers microhardness tester. The dentin conditions underneath the liners were also evaluated with FESEM. Statistical analysis was performed by two-way ANOVA and the post-hoc Games-Howell test (P<0.05). Results: Among the groups, the lowest microhardness value was in the control group (P<0.05) except at a depth of 100 μm; therefore, there was no significant difference between the control group and the bioactive glass (P>0.05). The laser group had the highest microhardness value, which was significantly different from the control group (P<0.05). There was a significant difference between the laser and bioactive glass (P<0.05), except at a depth of 20 μm. The laser and glass ionomer had only a significant difference at a depth of 100 μm (P<0.05). The microhardness value induced by glass ionomer was higher than bioactive glass, which in no depth was significant (P>0.05). Partial dentinal tubule occlusion was observed with FESEM in each of the experimental groups as compared to the control group. Conclusion: The microhardness values were higher in all groups than in the control group. The laser might be more successful in remineralization than the other ones.

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Effect of Remineralizing Agents on Tooth Color After Home Bleaching

Frontiers in Dentistry ◽

10.18502/fid.v16i3.1586 ◽

2019 ◽

Author(s):

Mohammadreza Malekipour ◽

Zahra Norouzi ◽

Sahar Shahlaei

Keyword(s):

Surface Treatment ◽

In Vitro Study ◽

Control Group ◽

Tooth Color ◽

Treatment Groups ◽

Tooth Discoloration ◽

Significant Difference ◽

The Mean ◽

Post Hoc

Objectives: The purpose was to investigate the effect of casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) paste, Remin Pro paste, and 0.05% sodium fluoride (NaF) mouthwash on teeth discoloration after home bleaching. Materials and Methods: In this in-vitro study, 48 intact and unstained human incisors were divided into four groups (n=12): control (Group 1), CPP-ACPF paste (Group 2), Remin Pro paste (Group 3), and 0.05% NaF mouthwash (Group 4). The specimens were bleached using 20% carbamide peroxide gel for 21 days and were subjected to pH cycling and surface treatment with remineralizing agents for one month. The color of the specimens was determined according to the CIELAB color space at baseline, after bleaching, and after surface treatment. Data were analyzed using one-way analysis of variance (ANOVA), post-hoc least significant difference (LSD) test, and one-sample t-test (P=0.05). Results: ANOVA showed a significant difference in the mean ΔE of the four groups after surface treatment (P<0.05). Post-hoc LSD test showed a significant difference between the mean ΔE of the control group and those of the treatment groups (P<0.05) with no significant difference between the treatment groups, except for Remin Pro. The mean ΔE and ΔL of the Remin Pro group showed less change than those of the CPP-ACPF and NaF groups, indicating that Remin Pro has the lowest potential for post-bleaching tooth discoloration. Conclusion: CPP-ACPF paste, Remin Pro paste, and 0.05% NaF mouthwash cause noticeable teeth discoloration immediately after bleaching. Remin Pro has less effect on tooth color than the other two products.

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Comparative Study to Evaluate the Surface Detail Reproduction of Dental Stone after Immersion in Various Different Disinfectant Solutions, Under Stereomicroscope 10 X Magnification –An In-Vitro Study

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i3.8316 ◽

2017 ◽

Vol 9 (3) ◽

Author(s):

Rathika Rai ◽

M. A. Easwaran ◽

K. T. Dhivya

Keyword(s):

Sodium Hypochlorite ◽

In Vitro Study ◽

Control Group ◽

Immersion Method ◽

Significant Difference ◽

Surface Irregularities ◽

Disinfectant Solution ◽

Surface Detail ◽

Group B

Aim: To evaluate the surface detail reproduction of dental stone this is immersed in different disinfectant solution and studied under stereomicroscope. Methodology: Total number of 30 specimens of dental stone (Type III) were made with measurements of 1.5cm diameter and 1cm height .This samples are divided in to 3 groups group A,B,C. were A is immersed in Distilled water which was taken as control group ;B is immersed in 2% Glutaraldehyde and C is immersed in 5%sodium hypochlorite. Each specimen were immersed in the disinfectant solution for 15 minutes and dried under room temperature for 24 hrs. After 24 hrs each specimens are studied under stereomicroscope for surface details. Result: The results showed no significant difference in the surface irregularities and porosities for a group 1 and group 2 except group 3 which showed significant increase in the porosities, surface irregularities and erosions after disinfection with 5% NaHOCl by immersion method. Conclusion: The surface detail reproduction capacity of die stone was adversely affected when 5% Sodium hypochlorite was used as disinfectant solution when compare d to control group and 2% Glutaraldehyde

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Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux

BMC Oral Health ◽

10.1186/s12903-021-01548-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Philipp Körner ◽

Luca Georgis ◽

Daniel B. Wiedemeier ◽

Thomas Attin ◽

Florian J. Wegehaupt

Keyword(s):

Gastroesophageal Reflux ◽

Artificial Saliva ◽

In Vitro Study ◽

Tooth Wear ◽

Control Group ◽

Erosive Tooth Wear ◽

Custom Made ◽

Post Hoc ◽

Bovine Enamel

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

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In vitro study of human osteoblast proliferation and morphology on orthodontic mini-implants

The Angle Orthodontist ◽

10.2319/100714-717.1 ◽

2015 ◽

Vol 85 (6) ◽

pp. 920-926 ◽

Cited By ~ 2

Author(s):

Ricardo Carvalho Bueno ◽

Roberta Tarkany Basting

Keyword(s):

Chemical Composition ◽

In Vitro Study ◽

Chemical Components ◽

Control Group ◽

Osteoblast Proliferation ◽

Mini Implants ◽

Significant Difference ◽

Implant Anchorage ◽

Positive Effect

ABSTRACT Objective: To evaluate the proliferation and morphology of human osteoblasts cultured on two brands of mini-implants after 24, 48, and 72 hours, in addition to the chemical composition found on their surface. Materials and Methods: Two brands of mini-implant (Morelli and Neodent) were evaluated; polystyrene was used as a control group (n = 3). Osteoblasts were cultured on the surface of sterilized mini-implants in a CO2 incubator at different time periods (24, 48, and 72 hours). Osteoblast proliferation was quantified by scanning electron microscopy using up to 5000× magnification, and cell morphology was analyzed by a single observer. For the chemical analysis, spectroscopy X-ray fluorescence was used to identify and quantify chemical components on the surface of the mini-implants. Results: Two-way ANOVA showed no significant interaction between the factors studied (P = 0.686). A Tukey test revealed no significant difference in osteoblast proliferation between the mini-implants at all studied periods; however, a difference in cell proliferation was detected between the Neodent and the control group (P = .025). For all groups, time had a direct and positive effect on osteoblast proliferation (P < .001). The significant elements present in both brands of mini-implants were titanium, aluminum, vanadium, and iron. Conclusions: Osteoblast proliferation was present on the mini-implants studied, which increased over time; however, no significant difference between brands was observed. No difference was seen between the mini-implants evaluated in terms of chemical composition. Cell adhesion after 72 hours suggests that areas of bone remodeling can be achieved, thus initiating the process of mini-implant anchorage.

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Milled versus moulded mock-ups based on the superimposition of 3D meshes from digital oral impressions: a comparative in vitro study in the aesthetic area

BMC Oral Health ◽

10.1186/s12903-019-0922-2 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 14

Author(s):

Francesca Cattoni ◽

Giulia Teté ◽

Alessandro Mauro Calloni ◽

Fabio Manazza ◽

Giorgio Gastaldi ◽

...

Keyword(s):

Statistical Analysis ◽

Test Group ◽

In Vitro Study ◽

Mean Value ◽

Digital Impression ◽

Control Group ◽

Minimal Invasiveness ◽

Significant Difference ◽

The Aesthetic

Abstract Background Aesthetic porcelain veneers proved to be a long-term reliable prosthetic solution, ensuring minimal invasiveness. The use of veneers requires an adhesive cementation technique, so maintaining as much enamel as possible is to ensure lasting success. A diagnostic mock-up is a key tool that allows a preview of the outcome of the aesthetic restoration: it is obtainable both in an analog and digital way. With the recent developments in impression technology and the ever so fast growing use of CAD-CAM technologies it is useful to understand the pros and cons of either one of these techniques (analog and digital) in order to identify the easier and more convenient workflow in aesthetic dentistry. Methods After taking pictures and impressions of the dental arcs of a patient in need of aesthetic rehabilitation, 52 resin models were produced and a digital drawing of the smile was outlined. Both an analog and a digital wax-up were obtained from two of the 52 models: the latter was obtained using digital impressions and a dedicated software. The analog wax-up was then used to produce 25 matrices that have later been used to mould 25 resin mock-ups using a traditional moulding protocol (Control Group - CG). The digital wax-up was used to mill 25 PMMA mock-ups. Each mock-up, both milled and moulded (total 50), was then laid on the other 50 resin models as a digital impression of it was taken. The STL. files of the milled mock-ups were compared with the 3D CAD wax-up made using a specific software. The STL. files of the analog printed mock-ups were compared with the traditional wax-up design. A statistical analysis was carried out to evaluate the difference between the groups. Results The statistical analysis showed a significant difference (P > 0.01) between the mean value of the distance between the points of the overlapping STL. meshes in GC (0.0468 mm) and in TG (Test Group - TG) (0,0109 mm). Conclusions The study showed a difference in accuracy between traditional moulded and milled mock-ups compared to their original wax-up. The data analysis reports that the digital method allows for greater accuracy. Within the limitations of this study, a fully digital workflow is to considered more reliable when it come to creating an esthetic mockup: the digital procedure has been shown to be more accurate than the one made manually which is much more operator dependent and it brings an increase to the chance of error, and that could ultimately affect the final result.

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The role of beluntas (pluchea indica less.) leaf extract in preventing the occurrence of fibroblasts hyperproliferation: An in vitro preliminary study

Dermatology Reports ◽

10.4081/dr.2019.8019 ◽

2019 ◽

Author(s):

Sakti Charlia Maharani ◽

Indah Julianto ◽

Suci Widhiati

Keyword(s):

Leaf Extract ◽

In Vitro Study ◽

Collagen I ◽

Control Group ◽

Antibody Treatment ◽

Average Decrease ◽

Matrix Deposition ◽

Post Hoc

Beluntas (Pluchea indica Less.) is a herbal plant which contains variety of benefits. Quercetin, one of flavonoid, is the most bioactive agent in beluntas leaf. Collagen inhibition by quercetin may modulate extracellular matrix deposition and inhibit the formation of hypertrophic scar. This was an in vitro study with senescent fibroblasts to determine the role of beluntas leaf extract in preventing the occurence of fibroblasts hyperproliferations. There were 4 groups were stained by anti-collagen I antibodies and secondary antibody. Flowcytometry analysis was done to measure the fibroblasts density. Anova test was performed with a value of p=0.000 (p<0.05). A post hoc analysis showed significant differences in the average decrease of fibroblasts that absorbs staining anti-collagen I antibody treatment group compared with the control group. There were significant effects of beluntas leaf extract in preventing the occurrence of fibroblasts hyperproliferations. Beluntas leaf extract with a concentration of 80 mol/L had the most significant effect on the fibroblasts density. Thus beluntas leaf extract has the ability in preventing the occurrence of fibroblasts hyperproliferation.

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Migration of fluoride ions from the permanent teeth into saliva in children with glass ionomer cement restorations: An in vitro study

Vojnosanitetski pregled ◽

10.2298/vsp1303279a ◽

2013 ◽

Vol 70 (3) ◽

pp. 279-283

Author(s):

Mirjana Apostolovic ◽

Biljana Kalicanin ◽

Marija Igic ◽

Olivera Trickovic-Janjic ◽

Dusan Surdilovic ◽

...

Keyword(s):

Artificial Saliva ◽

Fluorine Content ◽

In Vitro Study ◽

Vitro Study ◽

Permanent Teeth ◽

Glass Ionomer ◽

Fluoride Solution ◽

Low Concentration ◽

Significant Difference

Bacground/Aim. Glass ionomer cements (GIC) belong to the group of polycarboxyl cements, and one of the principal characteristics of these materials is their anticariogenic potential of fluorine release into saliva and enamel-dentin substance. The aim of this study was to examine the content of released fluorine from GIC restorations (Fuji IX, GC, Japan) of young permanent teeth in the medium of artificial saliva and similar releases in the same medium by the restorations of these teeth treated with a low concentration fluoride solution. Methods. We examined 12 premolars exctracted from orthodontic reasons. The GIC restored teeth were divided into the group treated daily with low concentration fluoride solution (334 ppm) and the control, not treated group. The samples of artificial saliva were analyzed for fluorine ion content using an ion selective electrode. Results. Our comparative analysis of the mean values using the Student?s t-test demonstrated a statistically significant difference in fluorine ion concentration in artificial saliva of fluoridated and non-fluoridated teeth with GIC fillings after 14 and 21 days (p < 0.05), while the difference detected after 7 days was with no statistical significance. Conclusion. The results of this in vitro study indicated that low-concentration fluoride solutions could serve to refluoridate GIC fillings and contribute to an increased fluorine content in saliva. The process of refluoridation of GIC fillings should be advised 2-3 weeks after the restoration, since the release of fluorine from GIC fillings diminishes in time.

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Cytotoxic Effect of Addition of Different Concentrations of Nanohydroxyapatite to Resin Modified and Conventional Glass Ionomer Cements on L929 Murine Fibroblasts

Frontiers in Dentistry ◽

10.18502/fid.v18i17.6248 ◽

2021 ◽

Author(s):

Parisa Golkar ◽

Ladan Ranjbar Omrani ◽

Shide Zohourinia ◽

Elham Ahmadi ◽

Faeze Asadian

Keyword(s):

Cytotoxic Effect ◽

In Vitro Study ◽

Biological Properties ◽

The Other ◽

Glass Ionomer Cements ◽

Glass Ionomer ◽

Beneficial Effects ◽

Significant Difference ◽

Murine Fibroblasts

Objectives: In this study we assessed the cytotoxic effect of nanohydroxyapatite (NHA) incorporated into resin modified and conventional glass ionomer cements (RMGICs and CGICs) on L929 murine fibroblasts. Materials and Methods: In this in vitro study, 0wt%, 1wt%, 2wt%, 5wt%, 7wt% and 10wt% concentrations of NHA were added to Fuji II LC RMGIC and Fuji IX CGIC powders. Eighteen samples (5×3mm) were fabricated from each type of glass ionomer, in six experimental groups (n=3): CG0, CG1, CG2, CG5, CG7, CG10, RMG0, RMG1, RMG2, RMG5, RMG7, and RMG10. Samples were incubated for 72h. The overlaying solution was removed and added to L929 fibroblasts. The methyl thiazolyl tetrazolium bromide (MTT) assay was performed at 24, 48 and 72h. The wavelength was read by a spectrophotometer. Data were analyzed by ANOVA and Tukey’s test. Results: There was no significant difference in cytotoxicity of the two types of glass ionomers, with and without NHA, except for CG0 and RMG0 groups after 72h. RMG0 group was significantly more cytotoxic than the CG0 group (P<0.05). In CG groups during the first 24h, the cytotoxicity of CG5 and CG7 groups was significantly higher than that of CG1; while, there was no significant difference between the RMG groups. Cytotoxicity significantly decreased in all groups after 24h (P<0.05). Conclusion: Incorporation of NHA into Fuji II LC RMGIC and Fuji IX CGIC did not affect their biocompatibility and therefore its addition to these materials can provide favorable biological properties, especially considering its beneficial effects on the other properties of GICs.

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The Effect of Glaze Coating on Wear Resistance of Some Acrylic Denture Teeth: An In Vitro Study

Avicenna Journal of Dental Research ◽

10.34172/ajdr.2021.09 ◽

2021 ◽

Vol 13 (2) ◽

pp. 47-51

Author(s):

Samaneh Abbasi ◽

Soodabe Ebrahimi ◽

Arash Shisheian ◽

Maryam Farhadian

Keyword(s):

Wear Resistance ◽

In Vitro Study ◽

Resistance Rate ◽

Vitro Study ◽

Wear Rates ◽

Denture Teeth ◽

Significant Difference ◽

The Mean ◽

Post Hoc

Background: Acrylic resin teeth wear resistance has an important role in the denture longevity. This study aimed to clarify the effect of glaze coating on wear resistance of three types of artificial acrylic teeth. Methods: In this in vitro study, the wear resistance rate of three of acrylic denture teeth (GENIUS, STON and CLASSIC) was compared with Ivoclar teeth (n=25/group). The wear resistance was measured by estimating the weight loss in pre and post removing glaze coating, following 5000 cycles in the chewing simulator device. Data analysis was made using paired t test, one-way ANOVA and Tukey’s post hoc test. Results: ANOVA test showed that there was no significant difference between the mean amount of wear of GENIUS, CLASSIC, STON and IVOCLAR teeth in the first stage (P <0.061), but this difference was significant (P <0.001) in the second stage. The result of Tukey post hoc test showed that wear rates of GENIUS were significantly lower than other groups (P<0.001). Comparison between the mean wear rates of each dental group at the first and second stages showed a significant difference between average teeth wear resistance of CLASSIC, STON and IVOCLAR in the first and second stages (P <0.001). Conclusions: In conclusion, the teeth wear resistance of STON and CLASSIC were similar to IVOCLAR. Also, after removing the glaze coating, the teeth wear resistance decreased in all groups but was not statistically significant for group GENIUS.

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