Gamma Radiation Induced Intestinal Proteomic Modulation in Mice: A Two Dimensional Electrophoretic Analysis

2017 ◽  
Vol 2 (3) ◽  
pp. 327 ◽  
Author(s):  
Sania Bajaj ◽  
Abhinav Singh ◽  
Bhargab Kalita ◽  
M.H. Yashavarddhan ◽  
Rajiv Ranjan ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Peptide Mass Fingerprinting ◽  
Electrophoretic Analysis ◽  
Two Dimensional ◽  
Peptide Mass ◽  
Damage Response ◽  
Radiation Induced ◽  
High Doses ◽  
Ionising Radiations ◽  
Serious Injuries

<p>Exposure to high doses of radiation causes serious injuries in gastrointestinal tract, by affecting biomolecules of the tissue. To demonstrate the modulation of intestinal proteome by ionising radiations, we analysed changes in protein expression in 9 Gy irradiated C57BL/6 mice at 24 h and 72 h by using two dimensional electrophoresis technique. A total of 19 protein spots with statistical significance (fold change&gt;1.5 and P&lt;0.05) were found to be differentially expressed. Of these 07 spots were identified by MALDI-TOF MS and peptide mass fingerprinting techniques which matched with the known proteins documented in the online database. These proteins belong to biological-functional categories like cytoskeleton system, molecular chaperones, DNA damage response, and stress response. These identified radiation induced proteins can help in understanding the mechanisms behind the intestinal injuries and thus can become potential targets for therapeutics and also aid in drug development.</p>

Two-dimensional electrophoretic analysis of proteins expressed by normal and cancerous human crypts: Application of mass spectrometry to peptide-mass fingerprinting

1994 ◽  
Vol 15 (1) ◽  
pp. 391-405 ◽  
Author(s):  
Hong Ji ◽  
Robert H. Whitehead ◽  
Gavin E. Reid ◽  
Robert L. Moritz ◽  
Larry D. Ward ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Peptide Mass Fingerprinting ◽  
Electrophoretic Analysis ◽  
Two Dimensional ◽  
Peptide Mass

scholarly journals Altered Protein Expression of Streptococcus oralis Cultured at Low pH Revealed by Two-Dimensional Gel Electrophoresis

2001 ◽  
Vol 67 (8) ◽  
pp. 3396-3405 ◽  
Author(s):  
Joanna C. Wilkins ◽  
Karen A. Homer ◽  
David Beighton
Keyword(s):  
Gel Electrophoresis ◽  
Peptide Mass Fingerprinting ◽  
Low Ph ◽  
Ionization Mass ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Altered Expression ◽  
Streptococcus Oralis ◽  
Peptide Mass

ABSTRACT Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH, soluble cellular proteins extracted from bacteria grown in batch culture at pH 5.2 or 7.0 were analyzed by two-dimensional (2-D) gel electrophoresis. Thirty-nine proteins had altered expression at low pH; these were excised, digested with trypsin using an in-gel protocol, and further analyzed by peptide mass fingerprinting using matrix-assisted laser desorption ionization mass spectrometry. The resulting fingerprints were compared with the genomic database forStreptococcus pneumoniae, an organism that is phylogenetically closely related to S. oralis, and putative functions for the majority of these proteins were determined on the basis of functional homology. Twenty-eight proteins were up-regulated following growth at pH 5.2; these included enzymes of the glycolytic pathway (glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase), the polypeptide chains comprising ATP synthase, and proteins that are considered to play a role in the general stress response of bacteria, including the 60-kDa chaperone, Hsp33, and superoxide dismutase, and three distinct ABC transporters. These data identify, for the first time, gene products that may be important in the survival and proliferation of nonmutans aciduric S. oralis under conditions of low pH that are likely to be encountered by this organism in vivo.

Sputtering Effects and Two Dimensional Arrangement of Nanoparticles in Insulators Under High Flux Cu Implantation

1999 ◽  
Vol 581 ◽  
Author(s):  
N. Kishimoto ◽  
C.G. Lee ◽  
N. Umeda ◽  
Y. Takeda ◽  
V.T. Gritsyna
Keyword(s):  
Dose Rate ◽  
High Flux ◽  
Two Dimensional ◽  
Cross Sectional ◽  
Near Surface ◽  
Self Assembling ◽  
Surface Nanostructures ◽  
Radiation Induced ◽  
High Doses ◽  
Surface Sputtering

ABSTRACTApplication of negative heavy ions, alleviating surface charging on insulators, enables us to conduct low-energy and high-flux implantation, and leads to a well-defined tool to fabricate near-surface nanostructures. Negative Cu ions of 60 keV, at high doses, have generated nanocrystals in amorphous(a-)SiO2 with a size (∼10 nm) suitable for nonlinear optical devices. The kinetic processes, inside the solid and at the surface, are studied by cross-sectional TEM and tapping AFM, respectively. In a-SiO2, nanoparticles spontaneously grow with dose rate, being controlled by the surface tension and radiation-induced diffusion. Furthermore, the nanospheres give rise to a two-dimensional (2D) arrangement around a given dose rate. The 2D-distribution occurs in coincidence with enhanced sputtering where a considerable Cu fraction sublimates from the surface. The dose-rate dependence of nanoparticles indicates that the surface-sputtering process influences the intra-solid process and contributes to the 2D-distribution. A self-assembling mechanism for 2D-arrangement of nanospheres is discussed taking into account contribution of the surface sputtering.

scholarly journals Proteomic Analysis of Azole Resistance in Candida albicans Clinical Isolates

2004 ◽  
Vol 48 (7) ◽  
pp. 2733-2735 ◽  
Author(s):  
Massoumeh Z. Hooshdaran ◽  
Katherine S. Barker ◽  
George M. Hilliard ◽  
Harald Kusch ◽  
Joachim Morschhäuser ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Peptide Mass Fingerprinting ◽  
Ergosterol Biosynthesis ◽  
Azole Resistance ◽  
Biosynthesis Pathway ◽  
Two Dimensional ◽  
Peptide Mass

ABSTRACT Changes in protein expression within a matched set of Candida albicans isolates representing the acquisition of azole resistance were examined by two-dimensional polyacrylamide gel electrophoresis and peptide mass fingerprinting. Proteins differentially expressed in association with azole resistance included Grp2p, Ifd1p, Ifd4p, Ifd5p, and Erg10p, a protein involved in the ergosterol biosynthesis pathway.

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