scholarly journals PERFIL FITOQUÍMICO, SUSCEPTIBILIDADE ANTIBACTERIANA E CAPACIDADE ANTIOXIDANTE DAS FOLHAS DE Croton urucurana Baillon (Euphorbiaceae)

2017 ◽  
Vol 29 (3) ◽  
pp. 264-270 ◽  
Author(s):  
Sarah Caroline De Souza Silva ◽  
Mônica Alcântara Alves ◽  
Sabrina Araujo De Sousa ◽  
José Ribamar De Souza Nogueira ◽  
Diegue Henrique Nascimento Martins ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Salmonella Thyphimurium

Croton urucurana Baillon, espécie conhecida popularmente como “sangria d’água”, pertence à família Euphorbiaceae, possui grande importância na medicina popular, a qual lhe atribui propriedades antibacterianas, anti-inflamatórias, antissépticas, antivirais, antidiarreicas, antissecretória intestinal e cicatrizante. Os objetivos deste estudo foram verificar a presença, nos extratos aquoso, etanólico e hidroalcoólico, de metabolitos secundários (alcaloides, antraquinonas, esteroides/triterpenoides, flavonoides, glicosídeos, saponinas e taninos), analisar a inibição do crescimento das bactérias Staphylococcus aureus ATCC 25923 e Salmonella thyphimurium ATCC 14028, e verificar a atividade antioxidante através do método DPPH. Para o perfil fitoquímico o melhor extrato foi o aquoso, quanto à susceptibilidade bacteriana, houve inibição com o extrato etanólico e o extrato com a maior atividade antioxidante foi o hidroalcóolico, apresentando IC50 1,3 μg/mL. Considerando os resultados obtidos no estudo, foi possível concluir que os extratos da Croton urucurana Baillon apresentam resultados interessantes e que mais investigações devem ser realizadas para a elucidação aprimorada dos seus benefícios.

scholarly journals Biodegradation of two organic UV-Filters by single bacteria strains

2021 ◽  
Author(s):  
Florentina Laura Chiriac ◽  
Catalina Stoica ◽  
Iuiana Paun ◽  
Florinela Pirvu ◽  
Toma Galaon ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Emerging Contaminants ◽  
Degradation Products ◽  
Aquatic Organisms ◽  
Uv Filters ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Biodegradation Process ◽  
Salmonella Thyphimurium ◽  
Organic Uv Filters

Abstract Organic UV-filters, including 4-hydroxybenzophenone (4-HBP) and 2,4-dihydroxybenzophenone (BP-1), are persistent emerging contaminants whose presence in the environment poses a threat to aquatic organisms due to their endocrine disruptor’s properties. For this reason, finding suitable technological processes for their safety and efficient removal from the environment represent a priority for the scientific community. To the author’s knowledge, until now, there are no studies reporting the biodegradation of 4-HBP and BP-1 by a single bacteria strain. In this paper, there were tested the 4-HBP and BP-1 biodegradation potential of two Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and two Gram-negative (Salmonella typhimurium and Serratia rubidae). The 4-HPB biodegradation process was observed only in the presence of Gram-negative bacterial strains. Thus, the biodegradation rates of 4-HBP reached up to 12.7% after 24h of incubation in presence of Salmonella thyphimurium and up to 24.0% after 24h of incubation with Serratia rubidae. Staphylococcus aureus was able to biodegrade 26.7% of BP-1, while Salmonella thiphymurium was able to biodegrade 14.7% of BP-1 after 24h of incubation. Their biodegradation products generated during the 4-HBP biodegradation process by Serratia rubidae were analyzed through LC-MS/MS analysis. The (bio)degradation products were benzophenone and a multi-hydroxylated derivative of 4-HBP and the degradation pathways were proposed. The data obtained in this study gave important information regarding the 4-HBP and BP-1 potential biodegradation by single bacterial strains.

scholarly journals Composição e atividades antioxidante e antimicrobiana dos óleos essenciais de cravo-da-índia, citronela e palmarosa

2009 ◽  
Vol 11 (4) ◽  
pp. 442-449 ◽  
Author(s):  
R. Scherer ◽  
R. Wagner ◽  
M.C.T. Duarte ◽  
H.T. Godoy
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Clostridium Perfringens ◽  
Cymbopogon Winterianus ◽  
Salmonella Thyphimurium ◽  
Cymbopogon Martinii

Foram avaliadas a ação antioxidante, ação antimicrobiana e a composição dos óleos essenciais de cravo-da-índia (Caryophillus aromaticus L.), citronela (Cymbopogon winterianus) e palmarosa (Cymbopogon martinii). A ação antioxidante foi avaliada pelo método de DPPH (2,2-difenil-1-picril-hidrazila), e a ação antimicrobiana determinada pelo método da microdiluição definindo-se a concentração inibitória mínima para os microrganismos Staphylococcus aureus, Escherichia coli, Salmonella Thyphimurium, Pseudomonas aeruginosa e Clostridium perfringens. A composição química dos óleos foi determinada por CG-DIC e a identificação dos compostos voláteis por CG-EM, em ambos os casos, utilizando uma coluna capilar DB-5. O óleo de cravo-da-índia apresentou uma forte atividade antioxidante e ação antimicrobiana moderada a forte, sendo o eugenol o componente majoritário do óleo de cravo-da-índia (83,7%). Por outro lado, as amostras de citronela e palmarosa apresentaram fraca ação antioxidante, porém a ação antimicrobiana foi moderada a forte.

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

Post-operative Staphylococcus aureus infection of a superficial femoral artery stent

VASA ◽  
2013 ◽  
Vol 42 (5) ◽  
pp. 382-386
Author(s):  
Karim Gariani ◽  
Marc Righini ◽  
Marco Roffi ◽  
Gino Gemayel ◽  
Damiano Mugnai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Femoral Artery ◽  
Superficial Femoral Artery ◽  
Aureus Infection
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