scholarly journals Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽  
2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Abdullah Abdullah ◽  
H. B. Mat

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

2010 ◽  
Vol 16 (1) ◽  
pp. 1 ◽  
Author(s):  
Abdullah Moch Busairi

Abdullah, in paper recycling of pineapple waste using lactobacillus delbroeckii to lactic acid, explain that The pineapple wastes juice contains mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer. The experiments were carried out in shake flask fermentation using Lactobacillus delbrueckii. Effect of some parameters such as temperature, initial pH, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65 % achieved at 40 o C, pH 6.00, 52.5 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10 %..


REAKTOR ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 50 ◽  
Author(s):  
Abdullah Abdullah

The liquid and solid pineapple waste contain mainly sucrose, glucose,fructose, and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer, the experiments were carried out in batch fermentation using the liquid and solid pineapple waste to produce lactic acid. The anaerobic fermentation of lactic acis were performed at 40 0C, ph 6, 5% inocolum, and 50 rpm. Initially results show that the liquid pineapple waste by using Lactobacillus delbrueckii can be used as carbon source for lactic acid fermentation. The production of lactic acid are found to be 79% yield, while only 56% yield was produced y using solid waste.Keywords : Lactic acid fermentation, Pineapple waste, Lactobacillus delbrueckii


2014 ◽  
Vol 695 ◽  
pp. 220-223
Author(s):  
Saber Salem Hassan ◽  
Roslinda Bt Abd Malek ◽  
Asliaty Atim ◽  
Suzi Salwah Jikan ◽  
Siti Fatimah Zaharah Mohd Fuzi

The fermentation process utilization to produce lactic acid has been studied from carbohydrate source and another source because of several significant reasons. Above all the production of biotechnology construction is found to be less costly compared to chemical synthesis. The production of biodegradable lactic polymer from lactic acid utilization of raw material can easily be obtained from industrial wastes such as pineapple waste. The process can positively affect the environment by reducing the environmental problems. The aim of this study is to estimate the effects of glucose concentration of pineapple wastewater as the carbon source on the volume ofLactobacilluscasei(L.casei) subspecies in producing lactic acid. Five different glucose concentrations as carbon source are used for production of high lactic acid in the fermentation process usingL.casei.L.caseicould be ingesting the glucose presented within the levels tested and converts all into lactic acid. The result shows efficient yields of 0.09 g lactic acid/g glucose. The highest level of lactic acid is at 125.71 g/l and was obtained from 100 % pineapple waste medium. When the carbon source is at 4 g/l, the level of lactic acid is decreased to 84.22 g/l. The fermentation time increases with the increment of sugars. It is more than double if the medium is composed of 100 % of pineapple waste. Therefore pineapple waste is the best alternative as carbon source for bacteria growth because it is more cost effective.


2021 ◽  
Author(s):  
Magdalena Lech

Abstract The brewery spent grain (BSG) is a lignocellulosic waste material produced in a huge amount around the world. Strict environmental protection law requires proper utilization. BSG can be transformed into easy-fermentable carbohydrates as a result of hydrolysis. This may be a low-cost raw material for biotechnological fermentation. The literature provides a lot of information that hydrolysis of lignocellulose creates by-products which can be potentially noxious to bacteria cells employed in fermentation. This research examined the influence of most of these by-products: furfural, acetic, formic, gallic, and levuilnic acid, on the LA fermentation effectiveness. These components were introduced to Lactobacillus cultures in various concentrations. The rate of cell growth, glucose consumption, and lactic acid production were measured. This components affects in various extent on LA formation in the culture. In any case, there is a critical value of them harms the fermentation, due to the reduced ability of bacteria propagation. Lower concentrations of bio-catalyst lead to an LA production efficiency drop. The LA concentrations in flasks after one day of propagation with the 2.1 [g/L] of appropriate inhibitor drop app. [%] 16 (F), 22 (GA), 8 (LevA), 40 (AA), and 100 (FA) in comparison to the flask without any inhibitor.


Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 111
Author(s):  
Mohamed T. Selim ◽  
Salem S. Salem ◽  
Amr Fouda ◽  
Mamdouh S. El-Gamal ◽  
Mohamed Ali Abdel-Rahman

Various challenges facing the industrial production of bio-based lactic acid (LA) such as cost of raw materials and nitrogen sources, as well as contamination risk by mesophilic and neutrophilic producers, should be overcome for the commercial production. This study aimed to investigate the feasibility of corn steep water (CSW) as a raw material for LA production using a newly thermo-alkali-tolerant lactic acid bacterium. The physicochemical characteristics of CSW were investigated. The high carbohydrates, proteins, amino acids, vitamins, essential elements, minerals, and non-protein nitrogenous compounds content confirmed that the CSW is a promising substrate for LA production. Out of 67 bacterial isolates, Enterococcus faecium WH51-1 was selected based on its tolerance to high temperatures and inhibitory compounds (sodium metabisulfate, sodium chloride, sodium acetate, and formic acid). Fermentation factors including sugar concentration, temperature, inoculum size, and neutralizing agents were optimized for LA production. Lactic acid concentration of about 44.6 g/L with a high yield (0.89 ± 0.02 g/g) was obtained using 60 g/L of CSW sugar, inoculum size 10% (v/v), 45 °C, and sodium hydroxide or calcium carbonate as a neutralizing agent. These results demonstrated the potential of strain WH51-1 for LA production using CSW effluent as raw material.


2007 ◽  
Vol 29 (12) ◽  
pp. 1973-1976 ◽  
Author(s):  
Solange I. Mussatto ◽  
Marcela Fernandes ◽  
Giuliano Dragone ◽  
Ismael M. Mancilha ◽  
Inês C. Roberto

2010 ◽  
Vol 113-116 ◽  
pp. 1302-1305 ◽  
Author(s):  
Jian Guo Liu ◽  
Qun Hui Wang ◽  
Hong Zhi Ma ◽  
Shuang Wang

To achieve high value-added utilization, vinasse was chosen as raw material for l-lactic acid production. Alkali methods, microwave were used to treat vinasse and the optimum parameters during the process were investigated. The results showed that the best pretreatment way was the combination of alkali concentration of 8% and microwave power 700W, corresponding maximum lactic acid concentration was 17.5g/L, higher than those of the independent method utilized, the fermentation time could be one day earlier than other methods. The conversion rate of preprocessed vinasse to L-lactic acid and utilization rate of cellulose and hemi-cellulose could reach 23.8% and 71%, respectively. The optimized pretreatment could effectively realize hydrolysis of vinasse and make it an ideal sources for further utilization.


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