Effects of Different Carbon Sources for High Level Lactic Acid Production by Lactobacillus casei

2014 ◽  
Vol 695 ◽  
pp. 220-223
Author(s):  
Saber Salem Hassan ◽  
Roslinda Bt Abd Malek ◽  
Asliaty Atim ◽  
Suzi Salwah Jikan ◽  
Siti Fatimah Zaharah Mohd Fuzi
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Fermentation Process ◽  
Lactic Acid Production ◽  
Industrial Wastes ◽  
Raw Material ◽  
Carbohydrate Source ◽  
Fermentation Time ◽  
Bacteria Growth ◽  
Pineapple Waste

The fermentation process utilization to produce lactic acid has been studied from carbohydrate source and another source because of several significant reasons. Above all the production of biotechnology construction is found to be less costly compared to chemical synthesis. The production of biodegradable lactic polymer from lactic acid utilization of raw material can easily be obtained from industrial wastes such as pineapple waste. The process can positively affect the environment by reducing the environmental problems. The aim of this study is to estimate the effects of glucose concentration of pineapple wastewater as the carbon source on the volume ofLactobacilluscasei(L.casei) subspecies in producing lactic acid. Five different glucose concentrations as carbon source are used for production of high lactic acid in the fermentation process usingL.casei.L.caseicould be ingesting the glucose presented within the levels tested and converts all into lactic acid. The result shows efficient yields of 0.09 g lactic acid/g glucose. The highest level of lactic acid is at 125.71 g/l and was obtained from 100 % pineapple waste medium. When the carbon source is at 4 g/l, the level of lactic acid is decreased to 84.22 g/l. The fermentation time increases with the increment of sugars. It is more than double if the medium is composed of 100 % of pineapple waste. Therefore pineapple waste is the best alternative as carbon source for bacteria growth because it is more cost effective.

scholarly journals Recycling Of Peneapple Waste Using Lactobacillus Delbroeckii to Lactic Acid

REAKTOR ◽  
2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Abdullah Abdullah ◽  
H. B. Mat
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Pineapple Waste ◽  
Fermentation Liquid ◽  
Initial Ph

The liquid pineapple waste contain mainly sucrose, glucose, fructose, and other nutrients. It therefore can potentiall be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradadable lactace polymer. The experiments weree carried out in shake flash fermentation using lactobacillus delbroeckii. Effect of some parameters such as temperature, initial Ph, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65% achieved at 40 0C, PH 6.00, 52.2 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10%.Keyword : lactic acid fermentation, liquid pineapple waste, lactobacillus delbrueckii

scholarly journals RECYCLING OF PINEAPPLE WASTE USING LACTOBACILLUS DELBROECKII TO LACTIC ACID

2010 ◽  
Vol 16 (1) ◽  
pp. 1 ◽  
Author(s):  
Abdullah Moch Busairi
Keyword(s):  
Lactic Acid ◽  
Yeast Extract ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Pineapple Waste ◽  
Initial Substrate ◽  
Initial Ph

Abdullah, in paper recycling of pineapple waste using lactobacillus delbroeckii to lactic acid, explain that The pineapple wastes juice contains mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for organic acid fermentation. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer. The experiments were carried out in shake flask fermentation using Lactobacillus delbrueckii. Effect of some parameters such as temperature, initial pH, initial substrate concentration, yeast extract concentration and fermentation time to the yield have been studied. The highest yield was 85.65 % achieved at 40 o C, pH 6.00, 52.5 g/l sugar concentration with 5 g/l yeast extract. There was no significant increasing in lactic acid production was observed if supplementation of yeast extract above 10 %..

scholarly journals Production of lactic acid by Enterococcus faecalis on waste glycerol from biodiesel production

2020 ◽  
Vol 26 (2) ◽  
pp. 151-156
Author(s):  
Jovan Ciric ◽  
Natasa Jokovic ◽  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
Dragisa Savic ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Enterococcus Faecalis ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Raw Material ◽  
Biodiesel Production ◽  
Waste Glycerol ◽  
Pure Glycerol ◽  
Microbial Utilization

Waste glycerol from biodiesel production is a valuable raw material that has been used to produce valuable microbial metabolites. In this work, the possibility of microbial utilization of waste glycerol obtained as a by-product in biodiesel production from sunflower and rapeseed oil by the lactic acid bacterium Enterococcus faecalis MK3-10A on a laboratory level was studied. For comparison, pure glycerol and glucose were used as carbon sources. The kinetics of the microbial biomass growth, the carbon source utilization, and the lactic acid production were monitored. The bacterium E. faecalis MK3-10A better grew in the media with glucose or pure glycerol as a carbon source, but the lactic acid production rate was the highest (14.6 mg/(ml/day)) in the medium with waste glycerol from the sunflower oil-based biodiesel production. Therefore, this waste glycerol might be a promising carbon source for lactic acidbacteria cultivation and lactic acid production.

scholarly journals Solid And Liquid Pineapple Waste Utilization For Lactic Acid Fermentation

REAKTOR ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 50 ◽  
Author(s):  
Abdullah Abdullah
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Batch Fermentation ◽  
Anaerobic Fermentation ◽  
Waste Utilization ◽  
Lactic Acid Fermentation ◽  
Lactobacillus Delbrueckii ◽  
Raw Material ◽  
Acid Fermentation ◽  
Pineapple Waste

The liquid and solid pineapple waste contain mainly sucrose, glucose,fructose, and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer, the experiments were carried out in batch fermentation using the liquid and solid pineapple waste to produce lactic acid. The anaerobic fermentation of lactic acis were performed at 40 0C, ph 6, 5% inocolum, and 50 rpm. Initially results show that the liquid pineapple waste by using Lactobacillus delbrueckii can be used as carbon source for lactic acid fermentation. The production of lactic acid are found to be 79% yield, while only 56% yield was produced y using solid waste.Keywords : Lactic acid fermentation, Pineapple waste, Lactobacillus delbrueckii

Effect of Pretreatment Methods on L-Lactic Acid Production from Vinasse Fermentation

2010 ◽  
Vol 113-116 ◽  
pp. 1302-1305 ◽  
Author(s):  
Jian Guo Liu ◽  
Qun Hui Wang ◽  
Hong Zhi Ma ◽  
Shuang Wang
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Value Added ◽  
Raw Material ◽  
Alkali Concentration ◽  
Utilization Rate ◽  
Fermentation Time ◽  
Optimum Parameters ◽  
Hydrolysis Of

To achieve high value-added utilization, vinasse was chosen as raw material for l-lactic acid production. Alkali methods, microwave were used to treat vinasse and the optimum parameters during the process were investigated. The results showed that the best pretreatment way was the combination of alkali concentration of 8% and microwave power 700W, corresponding maximum lactic acid concentration was 17.5g/L, higher than those of the independent method utilized, the fermentation time could be one day earlier than other methods. The conversion rate of preprocessed vinasse to L-lactic acid and utilization rate of cellulose and hemi-cellulose could reach 23.8% and 71%, respectively. The optimized pretreatment could effectively realize hydrolysis of vinasse and make it an ideal sources for further utilization.

scholarly journals Brewers’ spent grain as substrate for dextran biosynthesis by Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Prabin Koirala ◽  
Ndegwa Henry Maina ◽  
Hanna Nihtilä ◽  
Kati Katina ◽  
Rossana Coda
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Raw Materials ◽  
Degree Of Polymerization ◽  
Raw Material ◽  
Fermentation Time ◽  
Viscosity Increase ◽  
Spent Grain ◽  
Leuconostoc Pseudomesenteroides ◽  
Weissella Confusa

Abstract Background Lactic acid bacteria can synthesize dextran and oligosaccharides with different functionality, depending on the strain and fermentation conditions. As natural structure-forming agent, dextran has proven useful as food additive, improving the properties of several raw materials with poor technological quality, such as cereal by-products, fiber-and protein-rich matrices, enabling their use in food applications. In this study, we assessed dextran biosynthesis in situ during fermentation of brewers´ spent grain (BSG), the main by-product of beer brewing industry, with Leuconostoc pseudomesenteroides DSM20193 and Weissella confusa A16. The starters performance and the primary metabolites formed during 24 h of fermentation with and without 4% sucrose (w/w) were followed. Results The starters showed similar growth and acidification kinetics, but different sugar utilization, especially in presence of sucrose. Viscosity increase in fermented BSG containing sucrose occurred first after 10 h, and it kept increasing until 24 h concomitantly with dextran formation. Dextran content after 24 h was approximately 1% on the total weight of the BSG. Oligosaccharides with different degree of polymerization were formed together with dextran from 10 to 24 h. Three dextransucrase genes were identified in L. pseudomesenteroides DSM20193, one of which was significantly upregulated and remained active throughout the fermentation time. One dextransucrase gene was identified in W. confusa A16 also showing a typical induction profile, with highest upregulation at 10 h. Conclusions Selected lactic acid bacteria starters produced significant amount of dextran in brewers’ spent grain while forming oligosaccharides with different degree of polymerization. Putative dextransucrase genes identified in the starters showed a typical induction profile. Formation of dextran and oligosaccharides in BSG during lactic acid bacteria fermentation can be tailored to achieve specific technological properties of this raw material, contributing to its reintegration into the food chain.

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
By Products

Optimization of Potato Starch Residue L-Lactic Fermentation Culture Conditions

2011 ◽  
Vol 183-185 ◽  
pp. 1273-1277
Author(s):  
Zhong Xu ◽  
Nan Liu ◽  
Dan Zhao ◽  
Duo Wang
Keyword(s):  
Lactic Acid ◽  
Potato Starch ◽  
Culture Conditions ◽  
Raw Material ◽  
Optimal Dosage ◽  
Fermentation Time ◽  
Starch Concentration ◽  
Lactic Fermentation ◽  
Fermentation Temperature ◽  
Addition Level

Potato starch residue was used as raw material , a single factor test was used to determine the lactobacillus casei L-lactic fermentation in the amounts of CaCO3 addition, fermentation temperature, residue saccharification of starch concentration, the optimal dosage range of fermentation time. With 4 factors and 3 levels of 4 orthogonal test of L-lactic acid by fermentation. The order was: the fermentation temperature> saccharification concentration> fermentation period >CaCO3 dosage. Optimization was as follow : residue saccharification of starch concentration was 80g/L, fermentation temperature was 37°C, CaCO3 addition level was 60g/L, fermentation time was of 60h. Fermentation conditions for this verification test, L-lactic acid content was 72.3g/L, compared with 15.1% before optimization.

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