Long term treatment by mesenchymal stem cells conditioned medium modulates cellular, molecular and behavioral aspects of adjuvant-induced arthritis

There is little information on the fate of infused mesenchymal stem cells (MSCs) and long-term side effects after irradiation exposure. We addressed these questions using human MSCs (hMSCs) intravenously infused to nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice submitted to total body irradiation (TBI) or local irradiation (abdominal or leg irradiation). The animals were sacrificed 3 to 120 days after irradiation and the quantitative and spatial distribution of hMSCs were studied by polymerase chain reaction (PCR). Following their infusion into nonirradiated animals, hMSCs homed to various tissues. Engraftment depended on the dose of irradiation and the area exposed. Total body irradiation induced an increased hMSC engraftment level compared to nonirradiated mice, while local irradiations increased hMSC engraftment locally in the area of irradiation. Long-term engraftment of systemically administered hMSCs in NOD/SCID mice increased significantly in response to tissue injuries produced by local or total body irradiation until 2 weeks then slowly decreased depending on organs and the configuration of irradiation. In all cases, no tissue abnormality or abnormal hMSCs proliferation was observed at 120 days after irradiation. This work supports the safe and efficient use of MSCs by injection as an alternative approach in the short- and long-term treatment of severe complications after radiotherapy for patients refractory to conventional treatments.

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Targeting AMP ‐activated kinase impacts hepatocellular cancer stem cells induced by long‐term treatment with sorafenib

Molecular Oncology ◽

10.1002/1878-0261.12488 ◽

2019 ◽

Vol 13 (5) ◽

pp. 1311-1331 ◽

Cited By ~ 9

Author(s):

Alicia Bort ◽

Belén G. Sánchez ◽

Pedro A. Mateos‐Gómez ◽

Diana Vara‐Ciruelos ◽

Nieves Rodríguez‐Henche ◽

...

Keyword(s):

Stem Cells ◽

Cancer Stem Cells ◽

Hepatocellular Cancer ◽

Term Treatment ◽

Long Term Treatment

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Long-Term Treatment by Vitamin B1 and Reduction of Serum Proinflammatory Cytokines, Hyperalgesia, and Paw Edema in Adjuvant-Induced Arthritis

Basic and Clinical Neuroscience Journal ◽

10.15412/j.bcn.03070406 ◽

2016 ◽

Vol 7 (4) ◽

Cited By ~ 1

Author(s):

J. Zaringhalam ◽

A. Akbari ◽

A. Zali ◽

H. Manaheji ◽

V. Nazemian ◽

...

Keyword(s):

Proinflammatory Cytokines ◽

Vitamin B1 ◽

Term Treatment ◽

Paw Edema ◽

Adjuvant Induced Arthritis ◽

Long Term Treatment

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Analysis of the Effects of Low-Dose Radiation on Human Mesenchymal Stem Cells

Medical Radiology and radiation safety ◽

10.12737/1024-6177-2020-65-6-5-10 ◽

2021 ◽

Vol 65 (6) ◽

pp. 5-10

Author(s):

D. Usupzhanova ◽

T. Astrelina ◽

I. Kobzeva ◽

V. Nikitina ◽

Yu. Suchkova ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Conditioned Medium ◽

Adaptive Response ◽

Proliferative Activity ◽

Human Mesenchymal Stem Cells ◽

Conditioned Media ◽

X Ray ◽

Cultivation In Vitro

Purpose: The aim of the study was to study the effect of low X-ray doses on human mesenchymal stem cells (MSCs) in long-term cultivation in vitro. Material and methods: MSCs of the mucosal gum tissue of human were used. Cells were irradiated using an RUST-M1 X-ray unit (Russia) at doses of 50, 80, 100, 250 and 1000 mGy (dose rate40 mGy/min) and then cultivated according to standard methods. Immunological characteristics and viability of MSCs were evaluated on a FACSCanto II flow cytometer (Becton Dickinson CA, USA) for early and late passengers. Proliferative activity (PA) was evaluated using an xCelligence real-time cell analyzer (ACEA Biosciencs, Inc.). Results: It was shown that the proliferative activity (PA) of MSCs of the mucosal gum tissue which were irradiated at dose 50 mGy is comparable with the control group in long-term cultivation while doses of 100 and 250 mGy showed a decrease of PA. Also non-irradiated MSCs showed a significant decrease of the PA during cultivation in a conditioned medium from cells that received dose of 1000 mGy and an increase of PA during cultivation in a conditioned medium of cells that received doses of 50, 100 and 250 mGy. The cells were previously irradiated at dose 250 mGy showed adaptive response during cultivation in conditioned medium from cells that received dose of 1000 mGy. Сonclusion: The assessment of the effects of low radiation doses was focused on the bystander effect in the presented study. It was noted after adding conditioned media from irradiated cells to previously irradiated and non-irradiated MSCs. The bystander effects for low and high doses are different and their biological meaning requires further study. The phenomenon of adaptive response was shown after addition conditioned media from cells irradiated at dose 1000 mGy to pre-irradiated MSCs received a dose of 250 mGy. The obtained result leads to the conclusion that the effects of low doses can be positive. Thus, the results of study mainly correspond to the threshold nonlinear concept, according to which the effect is not proportional to the received radiation dose.

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Influence of 226 Ra on Bone Marrow Stem Cells in Mice: Effect of Radium Decorporation by a Long-Term Treatment with Na-Alginate on Stem-Cell Damage

Radiation Research ◽

10.2307/3575238 ◽

1980 ◽

Vol 82 (1) ◽

pp. 74 ◽

Cited By ~ 4

Author(s):

G. Schoeters ◽

S. Van Puymbroeck ◽

O. Vanderborght

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Stem Cell ◽

Cell Damage ◽

Term Treatment ◽

Bone Marrow Stem Cells ◽

Long Term Treatment

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Shedding Light on Targeting Chronic Myeloid Leukemia Stem Cells

Journal of Clinical Medicine ◽

10.3390/jcm10245805 ◽

2021 ◽

Vol 10 (24) ◽

pp. 5805

Author(s):

Mohammad Houshmand ◽

Alireza Kazemi ◽

Ali Anjam Najmedini ◽

Muhammad Shahzad Ali ◽

Valentina Gaidano ◽

...

Keyword(s):

Stem Cells ◽

Chronic Myeloid Leukemia ◽

Myeloid Leukemia ◽

Kinase Inhibitors ◽

Term Treatment ◽

Leukemia Stem Cells ◽

Hematopoietic Stem ◽

Long Term Treatment ◽

Treatment Free Remission

Chronic myeloid leukemia stem cells (CML LSCs) are a rare and quiescent population that are resistant to tyrosine kinase inhibitors (TKI). When TKI therapy is discontinued in CML patients in deep, sustained and apparently stable molecular remission, these cells in approximately half of the cases restart to grow, resuming the leukemic process. The elimination of these TKI resistant leukemic stem cells is therefore an essential step in increasing the percentage of those patients who can reach a successful long-term treatment free remission (TFR). The understanding of the biology of the LSCs and the identification of the differences, phenotypic and/or metabolic, that could eventually allow them to be distinguished from the normal hematopoietic stem cells (HSCs) are therefore important steps in designing strategies to target LSCs in a rather selective way, sparing the normal counterparts.

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Conditioned Medium of Mesenchymal Stem Cells Decreases Liver Fibrosis Caused by Acetaminophen by Suppressing the Systemic Inflammatory Response.

10.21203/rs.3.rs-418373/v1 ◽

2021 ◽

Author(s):

Andrey Temnov ◽

Alla Sklifas ◽

Vitaly Zhalimov ◽

Mars Sharapov ◽

Alexey Кodunov ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Inflammatory Response ◽

Conditioned Medium ◽

Functional Activity ◽

Liver Tissue ◽

Systemic Inflammatory Response ◽

Sterile Inflammation ◽

Long Term Effects

Abstract This study explored the effect of mesenchymal stem cells (MSC) conditioned medium (CM) on the severity of systemic inflammatory response caused by acetaminophen administration. The long-term effects of the toxin on the liver tissue were analysed. The effect of a fraction of the conditioned medium on the functional activity of isolated neutrophils was investigated using a model of sterile inflammation. The study showed that the >30 kDa CM fraction possesses the maximum protective effect. Proteins of this fraction reduce the severity of the systemic inflammatory reaction and the extent of liver tissue fibrosis after the toxin injection in the long term. By using the sterile inflammation model, CM was shown to reduce the complex activity of NADPH oxidase, which leads to a decrease in the total reactive oxygen species production. The CM derived from the cultivation of stem cells reduces the severity of the systemic inflammatory response through suppression of the functional activity of neutrophils.

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