scholarly journals The LBC Reaction (leuco-brilliant cresyl blue reaction), a New Method to Demonstrate Mitochondria in Living Cells

CYTOLOGIA ◽  
1964 ◽  
Vol 29 (4) ◽  
pp. 424-434 ◽  
Author(s):  
Sirô Tarao
Keyword(s):  
Living Cells ◽  
New Method ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Blue Reaction

scholarly journals ACCUMULATION OF BRILLIANT CRESYL BLUE IN THE SAP OF LIVING CELLS OF NITELLA IN THE PRESENCE OF NH3

1925 ◽  
Vol 9 (2) ◽  
pp. 235-253 ◽  
Author(s):  
Marian Irwin
Keyword(s):  
Ph Value ◽  
External Solution ◽  
Living Cells ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Rate Of Accumulation

When the living cells of Nitella are placed in a solution of brilliant cresyl blue containing NH4Cl, the rate of accumulation of the dye in the sap is found to be lower than when the cells are placed in a solution of dye containing no NH4Cl and this may occur without any increase in the pH value of the cell sap. This decrease is found to be primarily due to the presence of NH3 in the sap and seems not to exist where NH3 is present only in the external solution at the concentration used.

scholarly journals MECHANISM OF THE ACCUMULATION OF DYE IN NITELLA ON THE BASIS OF THE ENTRANCE OF THE DYE AS UNDISSOCIATED MOLECULES

1926 ◽  
Vol 9 (4) ◽  
pp. 561-573 ◽  
Author(s):  
Marian Irwin
Keyword(s):  
Total Concentration ◽  
Living Cells ◽  
Free Base ◽  
Brilliant Cresyl Blue ◽  
Rate Of Penetration ◽  
Cresyl Blue ◽  
Undissociated Molecule

The rate of penetration of brilliant cresyl blue into the living cells of Nitella indicates that the dye enters only in the form of the undissociated molecule. At equilibrium the total concentration of the dye in the sap is proportional to the concentration of the free base in the outside solution.

scholarly journals ON THE ACCUMULATION OF DYE IN NITELLA

1925 ◽  
Vol 8 (2) ◽  
pp. 147-182 ◽  
Author(s):  
Marian Irwin
Keyword(s):  
Temperature Coefficient ◽  
Living Cells ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Chemical Combination

Living cells of Nitella were placed in different concentrations of brilliant cresyl blue solutions at pH 6.9. It was found that the greater the concentration of the external dye solution, the greater was the speed of accumulation of the dye in the cell sap and higher was the concentration of dye found in the sap at equilibrium. Analysis of the time curves showed that the process may be regarded as a reversible pseudounimolecular reaction. When the concentration in the sap is plotted as ordinates and the concentration in the outside solution as abscissae the curve is convex toward the abscissae. There is reason to believe that secondary changes involving injury take place as the dye accumulates and that if these changes did not occur the curve would be concave toward the abscissae. The process may be explained as a chemical combination of the dye with a constituent of the cell. This harmonizes with the fact that the temperature coefficient is high (about 4.9). Various other possible explanations are discussed.

scholarly journals THE SUPRAVITAL STAINING OF VACCINE BODIES

1922 ◽  
Vol 36 (6) ◽  
pp. 667-684 ◽  
Author(s):  
Edmund V. Cowdry
Keyword(s):  
Salt Solution ◽  
Living Cells ◽  
Physiological Salt Solution ◽  
Low Grade ◽  
Similar Material ◽  
Structural Differentiation ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Corneal Cells ◽  
Supravital Staining

Vaccine bodies in living corneal cells may be specifically stained by the addition of a small quantity of brilliant cresyl blue 2 B to the physiological salt solution in which they are being observed. Their appearance by this method (Figs. 3 to 17) corresponds with that seen in fixed preparations (Figs. 22 to 42). Both lines of study reveal the existence of traces of similar material in unvaccinated corneal cells. As this increases in amount during the reaction, it behaves like an integral, cytoplasmic constituent of fluid consistency and shows no evidence of being endowed with any measure of independent vitality. The low grade of structural differentiation which it does exhibit, in living cells as well as in fixed tissues, is not suggestive of the presence within it of independent microorganisms. The material differs radically in its morphology and microchemical reactions from the granules observed by MacCallum and Oppenheimer in vaccine lymph.

scholarly journals EXIT OF DYE FROM LIVING CELLS OF NITELLA AT DIFFERENT pH VALUES

1926 ◽  
Vol 10 (1) ◽  
pp. 75-102 ◽  
Author(s):  
Marian Irwin
Keyword(s):  
Ph Value ◽  
External Solution ◽  
Living Cells ◽  
The Other ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Ph Values ◽  
Pass Through ◽  
External Ph

Experiments on the exit of brilliant cresyl blue from the living cells of Nitella, in solutions of varying external pH values containing no dye, confirm the theory that the relation of the dye in the sap to that in the external solution depends on the fact that the dye exists in two forms, one of which (DB) can pass through the protoplasm while the other (DS) passes only slightly. DB increases (by transformation of DS to DB) with an increase in the pH value, and is soluble in substances like chloroform and benzene. DS increases with decrease in pH value and is insoluble (or nearly so) in chloroform and benzene. The rate of exit of the dye increases as the external pH value decreases. This may be explained on the ground that DB as it comes out of the cell is partly changed to DS, the amount transformed increasing as the pH value decreases. The rate of exit of the dye is increased when the pH value of the sap is increased by penetration of NH3.

New method of holographic three-dimensional tracking of living cells exploiting their morphological properties

2013 ◽  
Author(s):  
Pasquale Memmolo ◽  
Maria Iannone ◽  
Maurizio Ventre ◽  
Paolo Antonio Netti ◽  
Andrea Finizio ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
New Method ◽  
Morphological Properties

scholarly journals Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

2003 ◽  
Vol 43 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Elisabeth Rodríguez-González ◽  
Manel López-Bejar ◽  
Dolors Izquierdo ◽  
María-Teresa Paramio
Keyword(s):  
Developmental Competence ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue

scholarly journals Effect of adding growth factors during in vitro maturation on the developmental potentials of ewe oocytes selected by brilliant cresyl blue staining

2021 ◽  
Vol 14 (2) ◽  
pp. 452-456
Author(s):  
Mohamed Fathi ◽  
Amr F. Elkarmoty
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Embryo Development ◽  
In Vitro Maturation ◽  
Blue Staining ◽  
Brilliant Cresyl Blue ◽  
Cresyl Blue ◽  
Nuclear Maturation ◽  
Maturation Medium

Aim: Several factors had been concerned with the developmental competence of the sheep oocyte. This study aims to investigate the effect of adding growth factors (insulin-like growth factor 1 [IGF-1] and epidermal growth factor [EGF]) in the maturation medium of ewe oocytes selected based on brilliant cresyl blue (BCB) screening on in vitro maturation (IVM), fertilization, and pre-implantation embryo development. Materials and Methods: Cumulus-oocyte complexes (COCs) were obtained from the ovaries of slaughtered ewes by either aspiration or slicing techniques. COCs were in vitro matured in a medium containing IGF-1 and EGF (control group). For BCB screening, oocytes were stained and divided into BCB+ oocytes that matured in the same maturation conditions without adding growth factors (Group 2) or in the presence of growth factors (Group 3), and BCB– oocytes that matured in medium without growth factors (Group 4) or with growth factors (Group 5). Results: The supplementation of the maturation medium with growth factors during IVM of (BCB+) oocytes resulted in a significant increase in nuclear maturation rate (90.9%), fertilization rate (75.6%), and embryo developmental rates (60.0%, 46.7%, and 33.3% for cleavage, morula, and blastocyst, respectively). Conclusion: Culturing BCB+ oocytes in a maturation medium containing both EGF and IGF-1 showed a significant improvement in nuclear maturation, fertilization, and pre-implantation embryo development in vitro.

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