scholarly journals Comparison of M.Semitendinosus Morphometry and Structure in Gilts and Barrows at Market Age

2016 ◽  
Vol 66 (1) ◽  
pp. 132-137
Author(s):  
Ivana Božičković ◽  
Duško Vitorović ◽  
Miloš Blagojević ◽  
Ivana Nešić ◽  
Slavoljub Jović ◽  
...  
Keyword(s):  
Cross Sectional Area ◽  
Histological Structure ◽  
Fiber Types ◽  
Sectional Area ◽  
Morphometric Characteristics ◽  
Cross Sectional ◽  
Fiber Number ◽  
Slow Twitch ◽  
Fast Twitch ◽  
Very High

AbstractThe aim of this paper was to investigate the differences in morphometric characteristics and histological structure of m. semitendinosus, between gilts and barrows of German Landrace breed at the end of the fattening period. Morphometric characteristics (weight, length, diameter and cross sectional area) of m. semitendinosus were not significantly different, while gender as a factor influenced the histological properties of this muscle. A very high statistical difference (p<0.01) in the total number of muscle fibers in m. semitendinosus was determined, with gilts having a higher total fiber number. The cross sectional area of fast twitch oxidative (FTO) fibers was significantly higher (p<0.05) in barrows. Gender did not affect the distribution of different fiber types: in both gender, the most present fiber types (48-52 %) were fast twitch glycolitic (FTG), FTO fibers represented 27-30 % of the total fiber number, while slow twitch oxidative fibers (STO) were the least represented (≈ 20 %).

Absence of regional differences in the size and oxidative capacity of diaphragm muscle fibers

1987 ◽  
Vol 63 (3) ◽  
pp. 1076-1082 ◽  
Author(s):  
G. C. Sieck ◽  
R. D. Sacks ◽  
C. E. Blanco
Keyword(s):  
Muscle Fibers ◽  
Continuous Distribution ◽  
Oxidative Capacity ◽  
Cross Sectional Area ◽  
Diaphragm Muscle ◽  
Type I ◽  
Fiber Types ◽  
Sectional Area ◽  
Cross Sectional ◽  
Fast Twitch

The oxidative capacity and cross-sectional area of muscle fibers were compared between the costal and crural regions of the cat diaphragm and across the abdominal-thoracic extent of the muscle. Succinate dehydrogenase (SDH) activity of individual fibers was quantified using a microphotometric procedure implemented on an image-processing system. In both costal and crural regions, population distributions of SDH activities were unimodal for both type I and II fibers. The continuous distribution of SDH activities for type II fibers indicated that no clear threshold exists for the subclassification of fibers based on differences in oxidative capacity (e.g., the classification of fast-twitch glycolytic and fast-twitch oxidative glycolytic fiber types). No differences in either SDH activity or cross-sectional area were noted between fiber populations of the costal and crural regions. Differences in SDH activity and cross-sectional area were noted, however, between fiber populations located on the abdominal and thoracic sides of the costal region. Both type I and II fibers on the abdominal side of the costal diaphragm were larger and more oxidative than comparable fibers on the thoracic side.

Long-term persistence of enlarged motor units in partially denervated hindlimb muscles of cats

1990 ◽  
Vol 64 (4) ◽  
pp. 1261-1269 ◽  
Author(s):  
A. R. Luff ◽  
K. Torkko
Keyword(s):  
Motor Units ◽  
Contralateral Side ◽  
Cross Sectional Area ◽  
Type I ◽  
Sectional Area ◽  
Motor Axons ◽  
Cross Sectional ◽  
Single Motor Units ◽  
Slow Twitch ◽  
Fast Twitch

1. It was the aim of this study to determine the effect that regenerating motor axons would have on enlarged or sprouted motor units that had been established for a relatively long time. 2. The fast-twitch flexor digitorum longus (FDL) and slow-twitch soleus were partially denervated by unilateral section of the L7 ventral root in 12-wk-old kittens. After 200+ days single motor units were isolated, and their isometric contractile properties were determined. FDL units were also tested for their resistance to fatigue and categorized as fast-twitch-fatigable (FF), fast-twitch-fatigue-resistant (FR), and slow-twitch-fatigue-resistant (S). 3. It had been established previously that regenerating motor axons via L7 returned to the experimental muscles by approximately 100 days. Therefore from 100 to 200 days it was assumed that the sprouted units would be in competition with the regenerating axons. The extent of the original denervations was variable and was estimated from the contralateral side. In soleus most denervations ranged from 83 to 99%; in FDL, from 37 to 81%. 4. In both soleus and FDL there was no evidence that the motor units had sprouted to any less extent than found previously. Within some soleus muscles the largest motor units were developing substantially more force than was expected. However, determination of mean fiber cross-sectional area from muscles frozen, sectioned, and prepared for histochemical analysis showed that this was attributable to increased mean cross-sectional area of the type I fibers.(ABSTRACT TRUNCATED AT 250 WORDS)

SDH activity and cross-sectional area of muscle fibers in cat diaphragm

1986 ◽  
Vol 60 (4) ◽  
pp. 1284-1292 ◽  
Author(s):  
G. C. Sieck ◽  
R. D. Sacks ◽  
C. E. Blanco ◽  
V. R. Edgerton
Keyword(s):  
Negative Correlation ◽  
Cross Sectional Area ◽  
Myosin Atpase ◽  
Sectional Area ◽  
Cross Sectional ◽  
Unimodal Distribution ◽  
Population Distributions ◽  
Slow Twitch ◽  
Slow Twitch Fibers ◽  
Fast Twitch

The succinate dehydrogenase (SDH) activity and cross-sectional area of individual muscle fibers in the cat diaphragm were quantified using a computerized image-processing system. The population distributions of fiber-SDH activities and cross-sectional areas showed considerable range within each diaphragm. Despite an overlap in the distribution of SDH activities between fast-twitch (i.e., those staining darkly for myosin ATPase) and slow-twitch (i.e., those staining lightly for myosin ATPase) fibers, differences between the two populations of fibers were observed. Fast-twitch fibers generally had lower SDH activities and greater cross-sectional areas than slow-twitch fibers. However, the range of SDH activities and cross-sectional areas of fast-twitch fibers was much greater than in slow-twitch fibers. The population distributions of SDH activities and cross-sectional areas of both fast- and slow-twitch fibers were unimodal. The unimodal distribution of SDH activities in fast-twitch fibers suggested that these fibers could not be clearly subdivided into two groups based on differences in their oxidative capacity (i.e., high- and low-oxidative fibers). These results were discussed in the context of the classification of fast-twitch glycolytic and fast-twitch oxidative-glycolytic fibers using qualitative histochemistry. In addition, the functional significance of the unimodal distribution of oxidative capacities in fast-twitch fibers was discussed in relation to the distribution of fatigue properties in fast-twitch motor units. A significant negative correlation between cross-sectional area and SDH activity in both fast- and slow-twitch fibers was also observed. The importance of this negative correlation in relationship to the diffusion of energy substrates for oxidative metabolism was discussed.

Intra- and inter-rater reliability for the measurement of the cross-sectional area of ankle tendons assessed by magnetic resonance imaging

2021 ◽  
pp. 028418512110032
Author(s):  
Henrique Mansur ◽  
Guilherme Estanislau ◽  
Marcos de Noronha ◽  
Rita de Cassia Marqueti ◽  
Emerson Fachin-Martins ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Repeated Measures ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Resonance Imaging ◽  
Cross Sectional ◽  
Rater Reliability ◽  
The Cross ◽  
Very High

Background The cross-sectional area (CSA) records make an essential measurement for determining the mechanical properties of tendons, such as stress and strength. However, there is no consensus regarding the best method to record the CSA from different tendons. Purpose To determine intra- and inter-rater reliability for CSA measures from magnetic resonance imaging (MRI) of the following tendons: tibialis anterior; tibialis posterior; fibularis longus and brevis; and Achilles. Material and Methods We designed an observational study with repeated measures taken from a convenience sample of 20 participants diagnosed with acute or chronic ankle sprain. Two independent raters took three separate records from the CSA of ankle tendon images of each MRI slice. The intra-class correlation coefficient (ICC) and 95% limits of agreement (LoA) defined the quality (associations) and magnitude (differences), respectively, of intra- and inter-rater reliability on the measures plotted by the Bland–Altman method. Results Data showed very high intra- and inter-rater correlations for measures taken from all tendons analyzed (ICC 0.952–0.999). It also revealed an excellent agreement between raters (0.12%–2.3%), with bias no higher than 2 mm2 and LoA in the range of 4.4–7.9 mm2. The differences between repeated measures recorded from the thinnest tendons (fibularis longus and brevis) revealed the lowest bias and narrowest 95% LoA. Conclusion Reliability for the CSA of ankle tendons measured from MRI taken by independent rates was very high, with the smallest differences between raters observed when the thinnest tendon was analyzed.

Regulation of fiber size, oxidative potential, and capillarization in human muscle by resistance exercise

1999 ◽  
Vol 276 (2) ◽  
pp. R591-R596 ◽  
Author(s):  
H. Green ◽  
C. Goreham ◽  
J. Ouyang ◽  
M. Ball-Burnett ◽  
D. Ranney
Keyword(s):  
Fiber Type ◽  
Vastus Lateralis ◽  
Succinic Dehydrogenase ◽  
Quadriceps Muscle ◽  
Cellular Tissue ◽  
Cross Sectional Area ◽  
Fiber Types ◽  
Sectional Area ◽  
Oxidative Potential ◽  
Cross Sectional

To examine the hypothesis that increases in fiber cross-sectional area mediated by high-resistance training (HRT) would result in a decrease in fiber capillarization and oxidative potential, regardless of fiber type, we studied six untrained males (maximum oxygen consumption, 45.6 ± 2.3 ml ⋅ kg−1 ⋅ min−1; mean ± SE) participating in a 12-wk program designed to produce a progressive hypertrophy of the quadriceps muscle. The training sessions, which were conducted 3 times/wk, consisted of three sets of three exercises, each performed for 6–8 repetitions maximum (RM). Measurements of fiber-type distribution obtained from tissue extracted from the vastus lateralis at 0, 4, 7, and 12 wk indicated reductions ( P < 0.05) in type IIB fibers (15.1 ± 2.1% vs. 7.2 ± 1.3%) by 4 wk in the absence of changes in the other fiber types (types I, IIA, and IIAB). Training culminated in a 17% increase ( P < 0.05) in cross-sectional area by 12 wk with initial increases observed at 4 wk. The increase was independent of fiber type-specific changes. The number of capillaries in contact with each fiber type increased by 12 wk, whereas capillary contacts-to-fiber area ratios remained unchanged. In a defined cross-sectional field, HRT also increased the capillaries per fiber at 12 wk. Training failed to alter cellular oxidative potential, as measured by succinic dehydrogenase (SDH) activity, regardless of fiber type and training duration. It is concluded that modest hypertrophy induced by HRT does not compromise cellular tissue capillarization and oxidative potential regardless of fiber type.

scholarly journals Morphological peculiarities of neuromuscular junctions among different fiber types: Effect of exercise

2017 ◽  
Vol 27 (3) ◽  
Author(s):  
Teet Seene ◽  
Maria Umnova ◽  
Priit Kaasik
Keyword(s):  
Axon Terminal ◽  
Neuromuscular Junctions ◽  
Muscle Fibers ◽  
Cross Sectional Area ◽  
Fiber Types ◽  
Sectional Area ◽  
Cross Sectional ◽  
Thin Sections ◽  
Axon Terminals ◽  
Ultrastructural Studies

The aim of our research was to examine whether there are differences in the morphology of neuromuscular junctions of different types of muscle fibers in rodents, and after their adaptation to six weeks endurance exercise training. After 5-day acclimation, Wistar rats were subjected to run with the speed 35 m/min during 6 week, 5 days per week and the training volume reached 60 min per day. Muscle samples for ultrastructural studies were fixed, dehydrated and embedded in Epon-812. Ultra-thin sections were cut from longitudinally and transversely oriented blocs, using 4 blocks from each animal. The area of axon terminals on fast- twitch fibers is 1.5 time large (p&lt;0.001) and the perimeter of terminals is 1.7 time large in comparison with slow- twitch oxidative fibers (p&lt;0.001) in control group. There are correlation between cross-sectional area of different muscle fibers and length of axon terminals (r=0.72), between cross-sectional area and with of axon terminal (r=-0.62), and between turnover rate of contractile proteins and length of axon terminal (r=0.75). Fast remodeling of synapse on oxidative and oxidative-glycolytic muscle fibers during endurance training seems to guarantees the intensive renewal of the structures of muscle fibers with higher oxidative capacity.

Muscle fiber formation and fiber hypertrophy during the onset of stretch-overload

1990 ◽  
Vol 259 (1) ◽  
pp. C92-C102 ◽  
Author(s):  
S. E. Alway ◽  
W. J. Gonyea ◽  
M. E. Davis
Keyword(s):  
Muscle Mass ◽  
Cross Sectional Area ◽  
Fiber Formation ◽  
Sectional Area ◽  
Cross Sectional ◽  
Fiber Splitting ◽  
Anterior Latissimus Dorsi ◽  
Fiber Number ◽  
Nitric Acid Digestion ◽  
Animal Control

The contributions of fiber hypertrophy and new fiber formation to the onset of stretch-induced muscle enlargement were evaluated in the anterior latissimus dorsi (ALD) of adult Japanese quails, because it was not known whether the mechanisms which initiate new fiber formation were dependent on first achieving significant fiber hypertrophy. A weight corresponding to 10% of the bird's body mass was attached to one wing, and eight birds were killed after each day during the first week of stretch. Muscle mass was significantly increased after 48 h of stretch; however, the elevation in nonmuscle tissue accounted for this increase. Muscle mass corrected for non-muscle tissue was significantly greater than the intra-animal control by the fourth day of stretch. Mean fiber cross-sectional area did not change during days 0-6, but cross-sectional area was 30.0 +/- 17.2% greater than the intra-animal control areas at day 7. Fiber number determined after nitric acid digestion of connective tissue was 27.1 +/- 5.8% greater than the intra-animal control at days 5-7 of stretch, but the number of fibers in the control muscles at days 5 and 6 were lower than at day 0. Thus new fiber formation was not preceded by significant fiber hypertrophy. These results fail to support a mechanism for new fiber formation which involves fiber splitting from hypertrophied myofibers during the first week of stretch.

scholarly journals Comparison of different skeletal muscles from growing domestic pigs and wild boars

2013 ◽  
Vol 56 (1) ◽  
pp. 766-777 ◽  
Author(s):  
D. Lösel ◽  
A. Franke ◽  
C. Kalbe
Keyword(s):  
Skeletal Muscles ◽  
Muscle Growth ◽  
Biochemical Properties ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Psoas Major ◽  
Fast Twitch

Abstract. The domestication process of pigs was associated with substantial morphological and physiological changes. The aim of this study was to comparatively investigate muscle microstructure and biochemical properties of four different skeletal muscles (M. longissimus, M. semitendinosus, M. psoas major, M. rhomboideus) in growing domestic pigs (n=5) and wild boars (n=4). The superiority of domestic pigs in body and muscle growth at three months of age was reflected by a 4-fold body weight and higher absolute weights of heart, liver and muscles. Relative muscle weights of M. psoas major and M. rhomboideus were reduced, whereas a 1.6-fold heavier M. semitendinosus was observed in domestic pigs compared with wild boars. In addition, the muscle cross-sectional area was greater in all muscles of domestic pigs resulting from about 3-fold fibre cross-sectional area, whereas total fibre number remained unchanged. As a consequence of intensified fibre hypertrophy, the fibre area per capillary is higher in all muscles of domestic pigs. Thus, the impaired supply with oxygen and nutrients may be one reason for the shift to the glycolytic muscle metabolism as indicated by a greater proportion of fast-twitch glycolytic fibres at the expense of fast-twitch oxidative fibres. This is also reflected by a higher ratio of lactate dehydrogenase to isocitrate dehydrogenase activity. Our data suggest that the intensified muscle growth was realized by myofibre hypertrophy at unchanged myofibre number in growing domestic pigs. It seems that domestication-induced changes were most pronounced in M. semitendinosus as the exterior ham muscle which may be related to muscle specific selection for lean growth.

scholarly journals Growth and Fruiting of Apple Trees on Dwarf and Semi-dwarf Rootstocks in Different High-density Orchards

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 473B-473
Author(s):  
A.S. Devyatov
Keyword(s):  
Fruit Quality ◽  
Cross Sectional Area ◽  
High Density ◽  
Normal Density ◽  
Sectional Area ◽  
Average Yield ◽  
Apple Trees ◽  
Cross Sectional ◽  
Very High ◽  
Dwarf Rootstocks

An orchard trial was established by planting an orchard with between-row intervals of 4 m. The French Axe was trained for trees with intervals in the row of 1 and 1.5 m. The hedgerow was used for treatments of 2–2.5 and 3 m between trees in the row. Semi-dwarf rootstock of Bud54-118 and dwarf one Bud62-396 were used. The growth of of these rootstocks was analogous to MM106 and M26, respectively. The trunk cross-sectional area of 7-year trees on 54-118 rootstock was 2.3 times more than on 62-396 for cv. Antey and 1.5 times more for cv. Tellisaare. The height of tree with French Axe crown at 7 years after planting on 54-118 rootstock reached 3.5–4 m. The height of tree was 0.5 m smaller on 62-396. The crown habit of tree on 62-396 rootstock was more comfortable for high -density orchard than trees on 54-118. The sum length of twigs that were cut out during 1993–96 to attain of normal density of crown was 2-4 times more than on 62-396 rootstock. Commercial fruiting of cv. Antey started at the 3rd leaf, but it was on 4th leaf for the more-dwarf rootstock 62-396. Average yield of fruit at 3–6 years after planting of cv. Antey for treatment of distance between trees in the row of 2 or 1.5 m was 6.8 kg/tree per year-1 for 54-118 rootstock, 3.4 and 3.5, respectively, for 62-396 rootstock. Yield at the 7th year after planting reached 24 and 32 kg on 54-118 rootstock, 16 and 15 kg on 62-396, respectively. Analogous date obtained for cv. Tellisa are. cv. Spartan on both rootstocks started to fruiting at 5-6 years after planting. The fruit quality was very high in all treatments of the trial.

Rat soleus muscle fiber responses to 14 days of spaceflight and hindlimb suspension

1992 ◽  
Vol 73 (2) ◽  
pp. S51-S57 ◽  
Author(s):  
Y. Ohira ◽  
B. Jiang ◽  
R. R. Roy ◽  
V. Oganov ◽  
E. Ilyina-Kakueva ◽  
...  
Keyword(s):  
Hindlimb Suspension ◽  
Protein Profiles ◽  
Sectional Area ◽  
Cross Sectional ◽  
Glycerophosphate Dehydrogenase ◽  
Rat Soleus Muscle ◽  
Slow Twitch ◽  
Fiber Atrophy ◽  
Slow Twitch Fibers ◽  
Fast Twitch

Morphological and enzymatic responses in fibers expressing fast, slow, or both types of myosin heavy chain (MHC) were studied in rats after 14 days of spaceflight (COSMOS 2044) or hindlimb suspension. Although the percentage of slow-twitch fibers was unchanged, a higher percentage of fibers that expressed both slow and fast MHC was observed in flight and suspended rats than in synchronous ground-based controls. The soleus was 25 and 34% smaller than control after 14 days of flight and suspension, with the reduction in fiber cross-sectional area (CSA) being greater in slow- than in fast-twitch fibers in both experimental groups. The activities of succinate dehydrogenase (SDH) and alpha-glycerophosphate dehydrogenase (GPD) were not significantly affected by flight or suspension. The total SDH activity (i.e., SDH activity x CSA) decreased significantly in the slow-twitch fibers of the flight and the fast-twitch fibers of the suspended rats, in large part due to fiber atrophy. A shift in MHC expression in 14 and 9% of the fibers in flight and suspended rats occurred without a change in myosin adenosinetriphosphatase activity. The SDH and GPD activities of the fibers that expressed both slow and fast MHC were slightly higher than the slow-twitch fibers and slightly lower than the fast-twitch fibers. These data indicate that events were initiated within 14 days of spaceflight or suspension that began to reconfigure the protein profiles of 9–14% of the slow-twitch fibers from typical slow-twitch toward those of fast-twitch fibers, while all fibers were dramatically losing total protein.(ABSTRACT TRUNCATED AT 250 WORDS)

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