Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and β-thalassemia minor

2005 ◽  
Vol 43 (2) ◽  
Author(s):  
José F. A. Noronha ◽  
Helena Z. W. Grotto
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Deficiency Anemia ◽  
Individual Values ◽  
Mean Cell Volume ◽  
Normal Individuals ◽  
Iron Deficient ◽  
Thalassemia Minor ◽  
Significant Difference ◽  
Red Blood Cell Indices

AbstractNew parameters correlated with the hemoglobin content in reticulocytes (RET-Y) and in red blood cells (RBC-Y) have been suggested as helpful in diagnosing iron deficiency anemia. We have studied RET-Y and RBC-Y indices in two groups of patients with microcytosis to verify if these parameters could be used to differentiate iron deficiency anemia from β-thalassemia minor. Blood samples from 33 iron-deficient patients, 25 β-thalassemic minor patients and 50 normal individuals were analyzed on a Sysmex XE-2100 instrument. A significant difference was observed in reticulocyte counting and immature reticulocyte fraction between iron deficiency anemia and β-thalassemia minor groups, but not in RBC-X and RET-Y parameters. Reticulocyte counting was higher in β-thalassemia minor and the immature reticulocyte fraction was higher in severe iron deficiency anemia. The ratio RET-Y/mean cell volume was tested and was significantly different when β-thalassemia minor was compared with mild and severe iron deficiency anemia, and showed better performance than the Mentzer ratio and the Green and King function. A great overlap of RET-Y and RBC-Y individual values was observed in both groups of microcytic anemias; we conclude that these new indices may be used with caution as indicative of iron deficiency, mainly in populations where β-thalassemia minor is frequent.

Proposal of a score combining red blood cell indices for early differentiation of beta‐thalassemia minor from iron deficiency anemia

Hematology ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Alexandre Janel ◽  
Laurence Roszyk ◽  
Chantal Rapatel ◽  
Gabrielle Mareynat ◽  
Marc G Berger ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Iron Deficiency Anemia ◽  
Red Blood Cell ◽  
Deficiency Anemia ◽  
Beta Thalassemia ◽  
Early Differentiation ◽  
Thalassemia Minor ◽  
Red Blood Cell Indices

scholarly journals DIFFERENCE OF HET RE LEVEL IN THALASSEMIA Β MINOR AND IRON DEFICIENCY ANEMIA

2020 ◽  
Vol 7 (11) ◽  
pp. 1-6
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Deficiency Anemia ◽  
Beta Thalassemia ◽  
Morphological Description ◽  
Control Measurement ◽  
Thalassemia Minor ◽  
Significant Difference ◽  
Hematological Analysis ◽  
North Sumatra

Background The most common diseases found with peripheral blood morphological features of hypochromic microcytic anemia are iron deficiency anemia and thalassemia. Hypochrome micrositer is a morphological description of red blood cells with MCV values smaller than normal (<80fl) and MCH smaller than normal values (<27pq). This morphological picture can be found in the condition of iron deficiency anemia and thalassemia. There are several markers for the assessment of Hb content in reticulocytes, including Ret-He. Ret-He, which can be measured by the latest automated hematological analysis, is considered to reflect the iron content in reticulocytes Aim Using Ret-He levels as a marker in distinguishing patients with β minor thalassemia and iron deficiency anemia. Method This research is an observational analytic study using case control measurement method, during April to July 2019 at the Department of Clinical Pathology, Faculty of Medicine, University of North Sumatra / H. Adam Malik General Hospital Medan. The population was students who were admitted to the University of North Sumatra who came for complete blood count examination. The subjects were students with MCV <80 fl, MCH <27 pq. Subjects who met the inclusion criteria then continued with the calculation of the Mentzer Index and RDW Index, ferritin serum examination using the ECLIA method, hemoglobin electrophoresis examination with micro capillary electrophoresis. By consecutive sampling, 42 samples were obtained, of which 21 subjects diagnosed with iron deficiency anemia and 21 subjects diagnosed with beta thalassemia minor were examined for their Ret-He levels using the flowsitometric method which were then analyzed statistically. Results From 21 iron deficiency anemia patients it was found that the average Ret-He value was 30.64 (6.08) pg and from 21 patients with beta minor thalassemia it was found that the average Ret-He value was 25.63 (6 , 72) pg. The results of the unpaired t-test for both groups obtained p = 0.016. By using the ROC curve obtained the Ret-He cut-off value in distinguishing cases of iron deficiency anemia with thalassemia was 27.30 pg with a sensitivity of 90.5% and specificity of 71.4%. Conclusion From the results of the study concluded that there was a significant difference between the levels of Ret-He in patients with iron deficiency anemia with thalassemia beta minor patients with a cut-off value of 27.30pg

scholarly journals Difference of Het Re Level In Thalassemia β Minor And Iron Deficiency Anemia

2020 ◽  
Vol 9 (2) ◽  
pp. 1635-1641
Author(s):  
Yustisia Amalia
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Complete Blood Count ◽  
Deficiency Anemia ◽  
Beta Thalassemia ◽  
Inclusion Criteria ◽  
Consecutive Sampling ◽  
Thalassemia Minor ◽  
Significant Difference ◽  
The University

The population was students who were admitted to the University of Dr. Soetomo who came for complete blood count examination. The subjects were students with MCV <80 fl, MCH <27 pq. Subjects who met the inclusion criteria then continued with the calculation of the Mentzer Index and RDW Index, ferritin serum examination using the ECLIA method, hemoglobin electrophoresis examination with micro capillary electrophoresis. By consecutive sampling, 42 samples were obtained, of which 21 subjects diagnosed with iron deficiency anemia and 21 subjects diagnosed with beta thalassemia minor were examined for their Ret-He levels using the flowsitometric method which were then analyzed statistically.From the results of the study concluded that there was a significant difference between the levels of Ret-He in patients with iron deficiency anemia with thalassemia beta minor patients with a cut-off value of 27.30pg

scholarly journals The effect of nutrition education based on PRECEDE model on iron deficiency anemia among female students

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ali Khani Jeihooni ◽  
Sanaz Hoshyar ◽  
Pooyan Afzali Harsini ◽  
Tayebeh Rakhshani
Keyword(s):  
Iron Deficiency ◽  
Nutrition Education ◽  
Blood Level ◽  
Iron Deficiency Anemia ◽  
Female Students ◽  
Deficiency Anemia ◽  
Fars Province ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

Abstract Background Iron deficiency anemia disrupts the concentration of adolescent girls; reduces their academic achievement, productivity, and physical strength, and increases the risk of infection. This research aim was to evaluate the effectiveness of the PRECEDE model nutrition education on iron deficiency anemia among female students of Fasa City, Fars Province, Iran. Methods This quasi-experimental study was done on 160 students (80 experimental and 80 control groups) who were selected using a random sampling method in Fasa City, Fars Province, Iran, in 2018–2019. The educational intervention included six sessions based PRECEDE model for 45 or 50 min. A scale of this study consisted of two parts; demographic information, and PRECEDE constructs were used to determine the nutritional behaviors status concluding preventing iron deficiency anemia and hemoglobin, hematocrit, and ferritin blood level in two (before and 4 months after intervention) times. Results In the experimental group of the students the mean age was 13.85 + 1.72 years and in the controlled group was 13.60 + 1.81 years. Moreover, there was no significant difference in the PRECEDE constructs, and nutritional behaviors preventing iron deficiency anemia before the intervention in two groups of study. However, the experimental group showed a significant increase 4 months after the intervention. Also, there was no significant difference in the mean score of hemoglobin, hematocrit, and ferritin blood level between the two groups before the intervention. However, in ferritin level, a significant increase was shown in 4 months after the intervention in the experiential group. Conclusions Based on results, the nutrition intervention education base on PRECEDE model has a positive effect to improve iron deficiency anemia preventive behaviors in female students.

scholarly journals Iron supplementation in goitrous, iron-deficient children improves their response to oral iodized oil

2000 ◽  
pp. 217-223 ◽  
Author(s):  
M Zimmermann ◽  
P Adou ◽  
T Torresani ◽  
C Zeder ◽  
R Hurrell
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Iron Supplementation ◽  
Thyroid Volume ◽  
Oral Iron ◽  
Deficiency Anemia ◽  
Urinary Iodine Concentration ◽  
Iodized Oil ◽  
Iron Deficient ◽  
Group 2

OBJECTIVE: In developing countries, many children are at high risk for both goiter and iron-deficiency anemia. Because iron deficiency may impair thyroid metabolism, the aim of this study was to determine if iron supplementation improves the response to oral iodine in goitrous, iron-deficient anemic children. DESIGN: A trial of oral iodized oil followed by oral iron supplementation in an area of endemic goiter in the western Ivory Coast. METHODS: Goitrous, iodine-deficient children (aged 6-12 years; n=109) were divided into two groups: Group 1 consisted of goitrous children who were not anemic; Group 2 consisted of goitrous children who were iron-deficient anemic. Both groups were given 200mg oral iodine as iodized oil. Thyroid gland volume using ultrasound, urinary iodine concentration (UI), serum thyroxine (T(4)) and whole blood TSH were measured at baseline, and at 1, 5, 10, 15 and 30 weeks post intervention. Beginning at 30 weeks, the anemic group was given 60mg oral iron as ferrous sulfate four times/week for 12 weeks. At 50 and 65 weeks after oral iodine (8 and 23 weeks after completing iron supplementation), UI, TSH, T(4) and thyroid volume were remeasured. RESULTS: The prevalence of goiter at 30 weeks after oral iodine in Groups 1 and 2 was 12% and 64% respectively. Mean percent change in thyroid volume compared with baseline at 30 weeks in Groups 1 and 2 was -45.1% and -21.8% respectively (P<0.001 between groups). After iron supplementation in Group 2, there was a further decrease in mean thyroid volume from baseline in the anemic children (-34.8% and -38.4% at 50 and 65 weeks) and goiter prevalence fell to 31% and 20% at 50 and 65 weeks. CONCLUSION: Iron supplementation may improve the efficacy of oral iodized oil in goitrous children with iron-deficiency anemia.

scholarly journals Prevalence of Iron Deficiency Anemia in Anemic Patients: A Hospital Based Study

2018 ◽  
Vol 6 (2) ◽  
pp. 41-45 ◽  
Author(s):  
Satyendra Kumar Mishra ◽  
Surendra Marasini ◽  
Badri Kumar Gupta ◽  
Krishna Kumar Agrawal ◽  
Narayan Gautam
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Serum Ferritin ◽  
Iron Status ◽  
Macrocytic Anemia ◽  
High Rate ◽  
Deficiency Anemia ◽  
Peripheral Smear ◽  
Significant Difference ◽  
Detailed Medical History

Introduction: In developing countries like Nepal, iron deficiency anemia (IDA) is one of the major concern. The high rate incidence has been related to insufficient  iron  intake, accompanied  by chronic  intestinal  blood  loss  due  to parasitic  and  malarial infections. Therefore, a study was conducted to evaluate the prevalence of IDA in anemic patients of Universal College of Medical Sciences-Teaching Hospital (UCMS-TH), South Western region, Nepal. Material and Method It was a hospital based cross sectional study comprised of 100 anemic patients. Their detailed medical history and lab investigations, focusing on hematological parameters were documented. Peripheral smear examination and serum ferritin estimation were done to observe red cell morphology and iron status respectively.  Results: This study revealed that out of 100 anemic patients, 35% were that of IDA. The most affected age group was 21-40 years with frequency 42.55%. IDA was more common in females (42.85%) than in male (21.62%). Out of 100 anemic patients, microcytic hypochromic anemia was predominant in 47% followed by macrocytic anemia (31%) and then normocytic normochromic anemia (22%). Out of 47 microcytic hypochromic anemic patients, 12 had normal serum ferritin. There was a statistical significant difference in Hb (p=0.011), MCV (p=0.0001), MCH (p=0.0001), MCHC (p=0.0001) and serum ferritin (p=0.0001) among all types of anemia. There was a statistical significant positive correlation of ferritin with Hemoglobin (0.257, p= 0.01), MCV (0.772, p= 0.0001), MCH (0.741, p=0.0001) and MCHC (0.494, p=0.0001).  Conclusion: The peripheral smear in conjunction with serum ferritin estimation needs to be included for susceptible individuals to screen the IDA and other types of anemia. 

scholarly journals Effects of Iron Deficiency Anemia and Its Rapid Correction on the Serum Metabolomic Profile of Rhesus Infants

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1070-1070
Author(s):  
Brian Sandri ◽  
Gabriele Lubach ◽  
Eric Lock ◽  
Michael Georgieff ◽  
Pamela Kling ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Acid Production ◽  
Iron Status ◽  
Deficiency Anemia ◽  
Iron Dextran ◽  
Metabolomic Profile ◽  
Intramuscular Injections ◽  
Iron Deficient ◽  
Rapid Correction

Abstract Objectives To determine whether rapid correction of iron deficiency using intramuscular iron dextran normalizes serum metabolomic changes in a nonhuman primate model of iron deficiency anemia (IDA). Methods Blood was collected from naturally iron-sufficient (IS; n = 10) and IDA (n = 12) male and female infant rhesus monkeys (Macaca mulatta) at 6 months of age. IDA infants were treated with intramuscular injections of iron dextran, 10 mg/weekly for 4–8 weeks. Iron status was reevaluated following treatment using hematological measurements and sera were metabolically profiled using HPLC/MS with isobaric standards for identification and quantification. Results Early-life iron deficiency anemia negatively affects many cellular metabolic processes, including energy production, electron transport, and oxidative degradation of toxins. Slow iron repletion with dietary supplementation restores iron deficient monkeys from a hematological perspective, but the serum metabolomic profile remains differed from monkeys that had been iron sufficient their entire life. Whether rapid iron restoration through intramuscular injections of iron dextran normalizes serum metabolomic profile is not known. A total of 654 metabolites were measured with differences in 53 metabolites identified between IS and IDA monkeys at 6 months (P 0.05). Pathway analyses provided evidence of altered liver function, hypometabolic state, differential essential fatty acid production, irregular inosine and guanosine metabolism, and atypical bile acid production in IDA infants. After treatment, iron-related hematological parameters had recovered, but the formerly IDA infants remained metabolically distinct from the IS infants, with 225 metabolites differentially expressed between the groups. Conclusions As with slow iron repletion, rapid iron repletion does not normalize the altered serum metabolomic profile in rhesus infants with IDA, suggesting the need for iron supplementation in the pre-anemic stage. Funding Sources National Institutes of Health.

Pseudothrombocytosis due to Microerythrocytosis: A Case of Beta Thalassemia Minor Complicated with Iron Deficiency Anemia

2013 ◽  
Vol 130 (2) ◽  
pp. 61-63 ◽  
Author(s):  
Sema Akinci ◽  
Tuba Hacibekiroglu ◽  
Abdulkadir Basturk ◽  
Sule Mine Bakanay ◽  
Tekin Guney ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Deficiency Anemia ◽  
Beta Thalassemia ◽  
Thalassemia Minor

The soluble transferrin receptor (sTfR)-ferritin index is a potential predictor of celiac disease in children with refractory iron deficiency anemia

2005 ◽  
Vol 43 (1) ◽  
Author(s):  
Maurizio De Caterina ◽  
Ernesto Grimaldi ◽  
Giovanni Di Pascale ◽  
Giuliana Salerno ◽  
Assunta Rosiello ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Transferrin Receptor ◽  
Hematological Parameters ◽  
Deficiency Anemia ◽  
Soluble Transferrin Receptor ◽  
Mean Cell Volume ◽  
Age Range ◽  
Normal Controls

AbstractThe soluble transferrin receptor (sTfR) distinguishes iron deficiency anemia from other types of anemia. Refractory iron deficiency anemia is often the onset symptom in malabsorption-induced celiac disease. We evaluated whether sTfR levels distinguish celiac disease-associated iron deficiency anemia from iron deficiency anemia of other origin. To this aim we measured sTfR and ferritin levels and their ratio (the sTfR/ferritin index) and other hematological parameters in 42 anemic children (20 with and 22 without celiac disease) vs. 22 non-anemic children with celiac disease and 31 healthy controls (age range 4–12years). Hemoglobin parameters, mean cell volume, and serum iron and ferritin levels were decreased to a similar extent in the anemic patients (celiac and non-celiac). The sTfR level in non-anemic celiac patients was similar to that of normal controls (1.7±0.35mg/L), whereas it was significantly increased in non-celiac and celiac anemic patients (2.2±0.5mg/L, p<0.05 and 2.7±1.2mg/L, p<0.001, respectively). The sTfR/ferritin index was also increased more in the anemic celiac patients (mean 4.4, range 1.5–12.0) than in anemic non-celiac children (mean 2.6, range 1.4–4.0) compared with non-anemic children (mean 1.2, range 0.7–2.0). Differences were more pronounced when ferritin was <5ng/mL. Thus, the sTfR/ferritin index may be a predictive measure in discriminating anemic patients with celiac disease from those without celiac disease.

scholarly journals OCCURRENCE OF IRON DEFICIENCY ANEMIA BETWEEN OVER WEIGHT AND NORMAL WEIGHT ADOLESCENTS IN SELECTED SCHOOLS IN UDUPI DISTRICT

2014 ◽  
Vol 04 (04) ◽  
pp. 033-038
Author(s):  
Sujatha R. ◽  
Reshma Reshma
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Normal Weight ◽  
Overweight And Obesity ◽  
Deficiency Anemia ◽  
Research Approach ◽  
Significant Difference ◽  
Overweight Adolescents

Abstract Background: Overweight has become one of the common health concerns. A few studies have noted a possible association between iron-deficiency anemia and overweight. As many as 3crore Indians are overweight, and obesity continues rise, says statistics revealed by the National Family Health Survey (NFHS 2010), Around 20% of school-going children are overweight. Materials and Methods: In this study survey research approach and comparative descriptive design was adopted. The sample size was 300 adolescents. 300 adolescents were selected by convenient sampling technique, out of which 225 were normal weight adolescents and remaining. Among 225 normal weight adolescents 50 adolescents were selected by simple random sampling technique (lottery method) and out of 75 overweight adolescents, 50 overweight adolescents were selected by convenient sampling technique. Initially BMI identified, Talliquis method was used for identifying the anemia. Peripheral smear test used for identifying the iron deficiency anaemia. Results: The data were analyzed by using descriptive and inferential statistics. The result shows that out of 300 adolescents75 (25%) of adolescents were overweight. Out of 100 children (50+50) 19(38%) were anemic in adolescents with overweight and seven (14%) were anemic in adolescents with normal weight. Out of the total 26 adolescents with anemia (19 -overweight and 7- normal weight) no occurrence of iron deficiency anemia was found. There is no significant difference in occurrence of anemia between overweight and normal weight adolescence. Interpretation and conclusion: The findings of the study indicates that majority of the overweight adolescents were anemic compare to normal-weight adolescents. There is no association between the occurrence of anemia and demographic variables like age, food habits, physical activity, menstrual problems etc.

Sign in / Sign up

Export Citation Format

Share Document