scholarly journals Early Sowing Reduces Cotton Leaf Curl Virus Occurrence and Improves Cotton Productivity

2015 ◽  
Vol 47 (4) ◽  
pp. 71-81 ◽  
Author(s):  
H. Ali ◽  
G.S. Hussain ◽  
S. Hussain ◽  
A.N. Shahzad ◽  
S. Ahmad ◽  
...  
Keyword(s):  
Insect Pests ◽  
Disease Incidence ◽  
Sowing Date ◽  
Seeding Density ◽  
Cotton Leaf ◽  
Sowing Dates ◽  
Cotton Leaf Curl Virus ◽  
Cotton Genotypes ◽  
Leaf Curl Virus ◽  
Leaf Curl

Abstract Cotton productivity is severely hampering by various diseases and insect pests especially cotton leaf curl virus (CLCV) worldwide. Losses caused by CLCV are far more than any other factor affecting cotton productivity. Growing of early and resistant genotypes is of vital significance in alleviating the adversities of these pests in crop plants. The current field trial was conducted at Central Cotton Research Institute (CCRI) Multan, Pakistan, to investigate the role of varying sowing dates in managing the CLCV infestation on different elite cotton genotypes. The crop was sown on five different dates i.e. D1= 15th April, D2 = 1st May, D3 = 15th May, D4 = 1st June and D5 = 15th June and three different elite cotton genotypes, i.e. V1= CIM-612, V2 = CIM-591 and V3 = CIM-573 to optimize a suitable sowing date and to screen out high productive and tolerant genotype against the CLCV. Seeds were drilled manually on finely crafted seedbed by using single row hand drill keeping seeding density of 20 kg ha-1 and inter row spacing of 75cm. CLCV severely hampered the crop performance by delayed planting of cotton from 15th April, while increased the chances of disease incidence. It is concluded that early sowing of all tested genotypes especially CIM-592 reduces the problem of CLCV and enhanced cotton productivity.

scholarly journals The Association of Begomovirus with Bitter Melon in India

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 328-328 ◽  
Author(s):  
J. A. Khan ◽  
M. K. Siddiqui ◽  
B. P. Singh
Keyword(s):  
Disease Incidence ◽  
Severe Disease ◽  
Cotton Leaf ◽  
Bitter Melon ◽  
Medicinal Value ◽  
Cotton Leaf Curl Virus ◽  
Pcr Products ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Bitter melon, Momordica charantia (Cucurbitaceae), is a vegetable of nutritive and medicinal value that is cultivated throughout India and other tropical countries. In September 2001, a severe disease of bitter melon with virus-like symptoms was observed at Lucknow, India. Symptoms consisted of upward curling, shortening, and distortion of leaves. Diseased melon fruits were stunted and deformed. Disease incidence was as high as 100%. Whitefly (Bemicia tabaci) can transmit the associated virus from diseased bitter melon to Nicotiana tabacum cv. White burley. The development of leaf curl symptoms in N. tabacum indicated the pathogen could be a begomovirus. Total nucleic acids were extracted from diseased bitter melon leaves, and polymerase chain reaction (PCR) tests were performed. Three pairs of primers, AV494 and AC1048 (1), CL-CR/F2 and CL-CR/R2, CL/11F and CL10/R (2), specific to DNA-A of begomoviruses were used in PCR. Virus-specific DNA-A fragments of expected sizes were identified (≈0.5, 0.7 and 1.2 kb, respectively). The presence of a begomovirus in all PCR-amplified DNA fragments was confirmed by Southern hybridization. Cloned DNA-A fragments of Tomato leaf curl virus and Cotton leaf curl virus (both begomoviruses) cross-hybridized with the PCR products gave strong signals under high stringency conditions. These data suggest that a begomovirus is associated with this bitter melon disease. Watermelon mosaic 1 virus is the only virus previously reported to naturally infect bitter melon; however, this virus has not been identified in India. Bitter melon is also an experimental host of Ribgrass mosaic virus (genus Tobamovirus) and Trichosanthes mottle virus (genus Potyvirus). To our knowledge, this is the first report of the occurrence of begomovirus infecting bitter melon. References: (1) S. D. Wyatt and J. K. Brown. Phytopathology 86:1288, 1996. (2). X. Zhou et al. J. Gen. Virol. 79:915, 1998.

Effect of Sowing Dates on Incidence of Cotton Leaf Curl Virus on Different Cultivars of Cotton

2004 ◽  
Vol 3 (2) ◽  
pp. 61-64 ◽  
Author(s):  
M. Tahir ◽  
M. Tariq . ◽  
H.T. Mahmood . ◽  
S. Hussain .
Keyword(s):  
Cotton Leaf ◽  
Sowing Dates ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Using Multiplexed CRISPR/Cas9 for Suppression of Cotton Leaf Curl Virus

2021 ◽  
Vol 22 (22) ◽  
pp. 12543
Author(s):  
Barkha Binyameen ◽  
Zulqurnain Khan ◽  
Sultan Habibullah Khan ◽  
Aftab Ahmad ◽  
Nayla Munawar ◽  
...  
Keyword(s):  
Genome Editing ◽  
Insect Pests ◽  
Restriction Analysis ◽  
Cotton Leaf ◽  
Cotton Production ◽  
Colony Pcr ◽  
Guide Rna ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

In recent decades, Pakistan has suffered a decline in cotton production due to several factors, including insect pests, cotton leaf curl disease (CLCuD), and multiple abiotic stresses. CLCuD is a highly damaging plant disease that seriously limits cotton production in Pakistan. Recently, genome editing through CRISPR/Cas9 has revolutionized plant biology, especially to develop immunity in plants against viral diseases. Here we demonstrate multiplex CRISPR/Cas-mediated genome editing against CLCuD using transient transformation in N. benthamiana plants and cotton seedlings. The genomic sequences of cotton leaf curl viruses (CLCuVs) were obtained from NCBI and the guide RNA (gRNA) were designed to target three regions in the viral genome using CRISPR MultiTargeter. The gRNAs were cloned in pHSE401/pKSE401 containing Cas9 and confirmed through colony PCR, restriction analysis, and sequencing. Confirmed constructs were moved into Agrobacterium and subsequently used for transformation. Agroinfilteration in N. benthamiana revealed delayed symptoms (3–5 days) with improved resistance against CLCuD. In addition, viral titer was also low (20–40%) in infected plants co-infiltrated with Cas9-gRNA, compared to control plants (infected with virus only). Similar results were obtained in cotton seedlings. The results of transient expression in N. benthamiana and cotton seedlings demonstrate the potential of multiplex CRISPR/Cas to develop resistance against CLCuD. Five transgenic plants developed from three experiments showed resistance (60−70%) to CLCuV, out of which two were selected best during evaluation and screening. The technology will help breeding CLCuD-resistant cotton varieties for sustainable cotton production.

scholarly journals Leaf cuticular wax content is involved in cotton leaf curl virus disease resistance in cotton (Gossypium hirsutum L.)

2019 ◽  
Vol 16 (4) ◽  
pp. e0705
Author(s):  
Muhammad Saeed ◽  
Song Xianliang ◽  
Sun Xuezhen ◽  
Muhammad Riaz
Keyword(s):  
Virus Disease ◽  
Cuticular Wax ◽  
Yield Potential ◽  
Cotton Leaf ◽  
Cotton Production ◽  
Cotton Leaf Curl Virus ◽  
Cotton Genotypes ◽  
Wax Content ◽  
Leaf Curl Virus ◽  
Leaf Curl

Cotton leaf curl virus disease (CLCuVD) limits cotton production in many cotton growing countries of the world, including Pakistan. In the past, efforts were made to combat this disease by different approaches. Cuticular wax is reported to confer resistance to plants against various biotic and abiotic stresses. Present study was designed to assess the role of cuticular wax content (WC) to resist CLCuVD infestation. The WC of 42 cotton genotypes, originating from various countries (Pakistan, USA, China, etc.), was quantified during two culture periods (2015 & 2016). Cotton germplasm was also scored for % disease index (%DI), seed cotton yield (SCY), number of bolls/plant (NB), and plant height (PHt) for the same culture periods. Significant negative correlation between WC and %DI was found during the two years of experimentation. WC was found positively correlated with SCY and NB. Six cotton genotypes (A-7233, B-557, A-162, BLANCO-3363, CIM-473, and SLH-2010-11) did not show any signs of CLCuVD infestation during both 2015 and 2016. These cotton genotypes contained relatively higher WC. The results from analysis of variance (ANOVA) demonstrated that there were significant differences among genotypes for %DI, WC, SCY, NB, and PHt. These results indicated that WC was involved in resisting CLCuVD and it also had positive effect on plant growth and yield potential. On the basis of these findings, it was concluded that cuticular wax could be used as an indirect criterion for distinguishing and selecting resistant/susceptible cotton genotypes.

scholarly journals Development of Cotton leaf curl virus resistant transgenic cotton using antisense ßC1 gene

2016 ◽  
Vol 23 (3) ◽  
pp. 358-362 ◽  
Author(s):  
Sayed Sartaj Sohrab ◽  
Mohammad A. Kamal ◽  
Abdul Ilah ◽  
Azamal Husen ◽  
P.S. Bhattacharya ◽  
...  
Keyword(s):  
Transgenic Cotton ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Exploration of Cotton Leaf Curl Virus (CLCuV) resistance genes and their screening in Gossypium arboreum by targeting resistance gene analogues

AoB Plants ◽  
2018 ◽  
Author(s):  
Rakhshanda Mushtaq ◽  
Khurram Shahzad ◽  
Shahid Mansoor ◽  
Zahid Hussain Shah ◽  
Hameed Alsamadany ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Cotton Leaf ◽  
Gossypium Arboreum ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Widespread Occurrence of Cotton leaf curl virus on Radish in Pakistan

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 809-809 ◽  
Author(s):  
S. Mansoor ◽  
S. Mukhtar ◽  
M. Hussain ◽  
I. Amin ◽  
Y. Zafar ◽  
...  
Keyword(s):  
Host Range ◽  
Full Length ◽  
Cotton Leaf ◽  
Degenerate Primers ◽  
Nylon Membrane ◽  
Punjab Province ◽  
Cotton Leaf Curl Virus ◽  
Kitchen Gardens ◽  
Leaf Curl Virus ◽  
Leaf Curl

The current epidemic of cotton leaf curl disease (CLCuD) in Pakistan started in 1988 with the natural host range limited to a few plant species in the family Malvaceae. However, we have observed expansion in the host range of the virus, and several non-Malvaceous plants were found to be infected with the virus. Characteristic symptoms of CLCuD such as leaf curl and enations have been observed on radish plants, primarily in kitchen gardens. However, in 1999, levels of infection of 10 to 90% were observed both in commercial fields and kitchen gardens in the Punjab province of Pakistan. Both symptomatic and nonsymptomatic samples were collected from five different locations. Total DNA was isolated, dot-blotted on nylon membrane, and a full-length clone corresponding to DNA A of cotton leaf curl virus was labeled with 32P dCTP and used as a probe for the detection of a begomovirus. Strong signals were observed in symptomatic plants while no signals were observed in nonsymptomatic plants. Infection with a begomovirus was further confirmed by polymerase chain reaction (PCR) using degenerate primers for DNA A (1). Primers specific for the two distinct begomoviruses associated with CLCuD were also used in PCR reactions (2), and products of the expected size were obtained from all symptomatic samples, confirming infection with begomoviruses similar to those associated with CLCuD. A full-length probe of a nanovirus-like molecule associated with cotton leaf disease (3), called DNA 1 was labeled with 32P dCTP and detected the virus only in symptomatic plants. Similarly, primers specific for DNA 1 (3) amplified a product of expected size when used in PCR. On the basis of symptomatology and the detection of specific viral components associated with the disease, we confirmed that radish plants are infected with Cotton leaf curl virus (CLCuV). Since radish is a short duration crop, infection of CLCuV in radish may not serve as a direct source of infection for the next cotton crop. However, it is a potential threat to tomato crops which overlap with radish in the Punjab province. The detection of CLCuD in radish is another example of the mobilization of begomoviruses to previously unknown hosts. References: (1) M. R. Rojas et al. Plant Dis. 77:340, 1993. (2) S. Mansoor et al. Pak. J. Bot. 31:115, 1999. (3) Mansoor et al. Virology 259:190, 1999.

scholarly journals Estimation of Genetic Effects Controlling Different Plant Traits in Cotton (Gossypium Hirsutum L.) Under Clcuv Epidemic Condition

2017 ◽  
Vol 50 (1) ◽  
pp. 47-56 ◽  
Author(s):  
A.M. Khan ◽  
S. Fiaz ◽  
I. Bashir ◽  
S. Ali ◽  
M. Afzal ◽  
...  
Keyword(s):  
Plant Traits ◽  
Fiber Strength ◽  
Block Design ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Fiber Fineness ◽  
Cotton Genotypes ◽  
Seed Index ◽  
Leaf Curl Virus ◽  
Additive Genes

Abstract Nine cotton genotypes, comprised of four non Bt males (CIM-1100, CIM-506, FH-942 and FH-900), five Bt female genotypes (FH-113, FH-114, MNH- 886, AA-703 and IR-3701) and their 20 crosses were screened in order to evaluate their response to Cotton leaf curl virus symptoms through epidemiology in the field and greenhouse. The 20 crosses among their nine parents were sown in two replications under randomized complete block design, during 2013 and 2014. The mean squares were significant for all traits indicating that both additive and non-additive genes control the characters, but non-additive genes were more important because, variance of dominant genes were higher than additive genes. In our test, FH-900 showed the best performance against CLCuV, number of lobes per boll and seed yield. The CIM- 1100 genotype performed well in boll weight, fiber strength and fiber fineness, whereas CIM-506 was good for plant height, number of sympodial branches and ginning. Maximum boll number, seed index and fiber length were shown by FH-942. Among lines, MNH886, FH-113, IR-3701 and FH-114 exhibited the best general combination for many traits. Hence, parents were preferred for hybridization program to improve the majority of characters. Hybrids FH-113 × FH-942, MNH-886 × CIM-1100, MNH-886 × FH-942, IR-3701 × CIM-506, AA-703 × CIM-1100, FH-114 × FH-942, FH-114 × CIM-1100 and MNH-886 × FH- 900 was best in specific combinations for different traits, especially against CLCuV. The results indicated that to increase resistance against CLCuV, hybrids should be exploited to develop CLCuV resistance.

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