Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

The enzyme biosynthesis using agricultural wastes by solid state fermentation (SSF) and the study of their physicochemical properties are meaningful approaches to improve the biomass hydrolysis. Among them, β-glucosidases and β-xylosidases are key enzymes at the lignocellulose depolymerization, which act in the cleavage of oligosaccharides in monosaccharides. In this study, the production of hemicellulases and cellulases by Pleurotus ostreatus and Aspergillus niger monocultures or in consortium was investigated, using raw sugarcane bagasse (SB) and wheat bran (WB) as substrates. The highest enzymatic activities were observed in the crude extract produced by P. ostreatus PLO6 and A. niger SCBM4 consortium with 98.5, 62.9, 3.8, 12.4, 13.3 and 20.2 U/g for β-glucosidase (β-glu), β-xylosidase (Bxyl), filter paper cellulase (FPase), xylanase (Xyl), exoglucanase (Exgl) and endoglucanase (Engl), respectively. The pH and temperature effects on β-glu and β-xyl were characterized. Optimal activities were obtained at pH 4.0 and 45 °C for β-glu and 3.5 and 55 °C for β-xyl. Both enzymes were stable at acid pH and presented thermostability. The results indicated that the enzymatic cocktail demonstrated potential characteristics for future applications in saccharifications. The use of sugarcane bagasse and wheat bran for microbial growth contributed to aggregate value to these byproducts.

Download Full-text

Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

Chemical Papers ◽

10.1515/chempap-2015-0111 ◽

2015 ◽

Vol 69 (8) ◽

Cited By ~ 10

Author(s):

Alesandra Oriente ◽

Robson Tramontina ◽

Diandra de Andrades ◽

Caroline Henn ◽

Jose L. C. Silva ◽

...

Keyword(s):

Aspergillus Niger ◽

Lignocellulosic Biomass ◽

Culture Conditions ◽

Industrial Wastes ◽

Fermentation Inhibitors ◽

State Culture ◽

Solid State Culture ◽

Atlantic Rainforest Biome ◽

Beta Glucosidase

AbstractProperties of beta-glucosidase produced by Aspergillus niger URM 6642 recently isolated from the Atlantic rainforest biome and its potential tolerance to saccharification of lignocellulosic biomass products and fermentation inhibitors was evaluated. The fungus was cultivated under solid state culture conditions at 37°C with different agro-industrial wastes. High levels of beta-glucosidase (3778.9 U g

Download Full-text

Isolation and partial characterization of alkaline feruloyl esterases from Aspergillus niger CFR 1105 grown on wheat bran

World Journal of Microbiology and Biotechnology ◽

10.1007/s11274-009-0095-2 ◽

2009 ◽

Vol 25 (11) ◽

pp. 1963-1969 ◽

Cited By ~ 25

Author(s):

Shyamala Hegde ◽

Gudipati Muralikrishna

Keyword(s):

Aspergillus Niger ◽

Wheat Bran ◽

Partial Characterization ◽

Feruloyl Esterases

Download Full-text

Production and Characterization of Xylanase from Aspergillus niger using Wheat Bran, Corn Cobs, and Sugar Cane Bagasse as Carbon Sources with Different Concentrations

Journal of Bioresource Management ◽

10.35691/jbm.6102.0040 ◽

2016 ◽

Vol 3 (1) ◽

Keyword(s):

Aspergillus Niger ◽

Wheat Bran ◽

Sugar Cane ◽

Carbon Sources ◽

Sugar Cane Bagasse ◽

Corn Cobs

Download Full-text

Production and characterization of a thermostable β-glucosidase from Myceliophthora heterothallica

Bioscience Journal ◽

10.14393/bj-v36n1a2020-47758 ◽

2020 ◽

Vol 36 (1) ◽

Author(s):

Maria Eduarda da Mata Martins ◽

Eduardo da Silva Martins ◽

Heytor Lemos Martins

Keyword(s):

Solid State ◽

Nutrient Solution ◽

Sugarcane Bagasse ◽

Wheat Bran ◽

Incubation Temperature ◽

Solution Ph ◽

Industrial Residues ◽

Initial Substrate ◽

Substrate Moisture

The conversion of biomass from agro-industrial residues into bioproducts is of great interest, especially to Brazil, where bioenergy has a huge potential for development. Enzymes involved in biodegradation of lignocellulosic biomass are those of the cellulase system, of which β-glucosidase is a constituent. The production and characterization of β-glucosidase by the thermophilic fungus Myceliophthora heterothallica by solid-state cultivation on different agro-industrial residues (sugarcane bagasse, sugarcane straw, wheat bran and a mixture of these three materials (1:1:1 w/w) was evaluated. Solid-state cultivation were conducted in 250 mL Erlenmeyer flasks, with 5 g of each substrate. Different culture parameters, such as supplementary nutrient solution to the substrate, supplementary nutrient solution pH, initial substrate moisture and fungus incubation temperature, were evaluated to establish conditions of higher enzyme production by the fungus The greatest production of enzymes occurred in a mixture of wheat bran, sugarcane bagasse and straw bagasse (1:1:1). The activity of β-glucosidase was greater under the following conditions: nutrient solution composed of NH4NO3, MgSO4.7H2O and (NH4)2SO4 (0.1%), at pH 4.5 or 6.0, fungus incubation at 40°C or 45°C, initial moisture of substrate at 80%. Enzyme presented optimum pH at pH 5.0 and good pH stability. Best temperature was 65°C and enzyme showed 100% stability for 1h, up to 60°C. The use of agro-industrial residues provided good production of β-glucosidase by fungus, with enzyme having the characteristics desirable from the industrial application.

Download Full-text

BAGAÇO DE MALTE COMO SUPLEMENTO NUTRICIONAL NO CULTIVO DE Pleurotus ostreatus

ENERGIA NA AGRICULTURA ◽

10.17224/energagric.2021v36n1p155-164 ◽

2021 ◽

Vol 36 (1) ◽

pp. 155-164

Author(s):

Ana Carolina Marques de Oliveira ◽

Márcia Tidemann ◽

Olívia Gomes Martins ◽

Meire Cristina Nogueira de Andrade

Keyword(s):

Wheat Bran ◽

Pleurotus Ostreatus ◽

Chemical Characterization ◽

Nutritional Supplement ◽

Agronomic Performance ◽

Fruiting Bodies ◽

Average Mass ◽

E Mail ◽

Eucalyptus Sawdust

BAGAÇO DE MALTE COMO SUPLEMENTO NUTRICIONAL NO CULTIVO DE Pleurotus ostreatus ANA CAROLINA MARQUES DE OLIVEIRA1, MÁRCIA TIDEMANN2, OLÍVIA GOMES MARTINS3, MEIRE CRISTINA NOGUEIRA DE ANDRADE4 1 Graduanda em Ciências Biológicas, Centro Universitário Sagrado Coração – Unisagrado, R. Irmã Arminda, 10-50 - Jardim Brasil, 17011-160 Bauru, SP, Brasil. E-mail: [email protected] 2 Tecnóloga em Produção Industrial, Faculdade de Tecnologia de Botucatu (FATEC), Av. José Ítalo Bacchi, s/n - Jardim Aeroporto, 18606-851, Botucatu, SP, Brasil. E-mail: [email protected] 3 Doutoranda em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 4 Docente da Faculdade Gran Tietê, Av. 15 de Novembro, 125 - Centro, 17340-000, Barra Bonita, SP, Brasil. E-mail: [email protected] RESUMO: O estudo objetivou avaliar o uso do bagaço de malte para cultivar duas linhagens de P. ostreatus (MB e SB), avaliando a caracterização química dos substratos, a massa e o número de cachos produzidos. Os substratos foram preparados à base de bagaço de cana-de-açúcar e serragem de eucalipto, suplementados com farelo de trigo (suplementação tradicional) ou bagaço de malte em substituição ao farelo em diferentes proporções (S1=0%, S2=10% e S3=20%). A linhagem SB resultou em massa média de 163,70 g e 22,66 cachos, superior à linhagem MB, com média de 121,70 g e 17,54 cachos. O substrato S1 resultou em uma massa média de 196,08 g e 28,62 cachos, o S2 em 154,20 g e 19,50 cachos e o S3 em 77,81 g e 12,18 cachos. Os resultados sugerem que suplementar o substrato com bagaço de malte pode afetar características químicas do substrato. A suplementação com até 10% de bagaço de malte, em comparação com a suplementação tradicional (farelo de trigo), não afetou significativamente a massa produzida pelo P. ostreatus, mas diminuiu o número de cachos. A linhagem SB obteve desempenho agronômico superior à linhagem MB. Palavras-chave: aproveitamento, resíduos, produtividade, cogumelos. MALT BAGASSE AS A NUTRITIONAL SUPPLEMENT IN THE CULTIVATION OF Pleurotus ostreatus ABSTRACT: The study aimed to evaluate the use of malt bagasse to cultivate two strains of P. ostreatus (MB and SB), evaluating the chemical characterization of the substrates, the mass and the number of bunches of fruiting bodies produced. The substrates were prepared based on sugarcane bagasse and eucalyptus sawdust, supplemented with wheat bran (traditional supplementation) or malt bagasse in substitution to the bran in different proportions (S1=0%, S2=10% and S3=20%). The SB strain resulted in an average mass of 163.70 g and 22.66 bunches, superior to the the MB strain, with an average of 121.70 g and 17.54 bunches. The substrate S1 resulted in an average mass of 196.08 g and 28.62 bunches, S2 in 154.20 g and 19.50 bunches and S3 in 77.81 g and 12.18 bunches. The results suggest that supplementing the substrate with malt bagasse may affect the chemical characteristics of the substrate. Supplementation with up to 10% malt bagasse, compared to traditional supplementation (wheat bran), did not significantly affect the mass produced by P. ostreatus, but decreased the number of bunches of fruiting bodies. The SB strain obtained an agronomic performance superior to the MB strain. Keywords: repurposing, residues, productivity, mushrooms.

Download Full-text

Characterization of laccase isoforms produced by Pleurotus ostreatus in solid state fermentation of sugarcane bagasse

Bioresource Technology ◽

10.1016/j.biortech.2012.03.058 ◽

2012 ◽

Vol 114 ◽

pp. 735-739 ◽

Cited By ~ 55

Author(s):

Susan Grace Karp ◽

Vincenza Faraco ◽

Antonella Amore ◽

Leila Birolo ◽

Chiara Giangrande ◽

...

Keyword(s):

Solid State ◽

Sugarcane Bagasse ◽

Solid State Fermentation ◽

Pleurotus Ostreatus ◽

Laccase Isoforms

Download Full-text

Reuse of degraded Pleurotus ostreatus substrate through supplementation with wheat bran and Calprozime® quantitative parameters

Agronomía Colombiana ◽

10.15446/agron.colomb.v33n2.49760 ◽

2015 ◽

Vol 33 (2) ◽

pp. 261-270 ◽

Cited By ~ 2

Author(s):

Mª Raquel Picornell ◽

Arturo Pardo ◽

José Arturo De Juan-Valero†

Keyword(s):

Wheat Bran ◽

Pleurotus Ostreatus ◽

Base Material ◽

Chemical Characterization ◽

Unit Weight ◽

Biological Efficiency ◽

Fruiting Bodies ◽

Physical And Chemical Characterization ◽

Physical And Chemical

In this study, the agronomic viability of Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. was studied by reusing spent substrates that were previously used for crops of this mushroom. After the physical and chemical characterization of the substrates, we evaluated the quantitative production parameters for one growing season. The experiment used wheat straw (WS) and spent Pleurotus substrate (SPS) as a base material to generate prepared substrates that could be reutilized for mushroom production. These base substrates were supplemented with wheat bran (WB) (two doses, 300 and 600 g/6 kg) and the commercial supplement Calprozime® (120 g/6 kg). We obtained a biological efficiency (BE) between 50 and 63%, a high quantity of mushrooms (between 26 and 39 mushrooms/bag) and an excellent unit weight of the fruiting bodies (between 24.34 and 39.54 g) with the substrates supplemented with a 120 g/6 kg dose of Calprozime®.

Download Full-text

Characterization of carbides in M50NiL

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100087343 ◽

1991 ◽

Vol 49 ◽

pp. 606-607

Author(s):

L. S. Lin ◽

K. P. Gumz ◽

A. V. Karg ◽

C. C. Law

Keyword(s):

Temperature Effects ◽

Base Composition ◽

Lattice Parameter ◽

Control Surface ◽

Carbide Formation ◽

Particle Sizes ◽

Low Carbon ◽

Fee Structure ◽

Heat Treated

Carbon and temperature effects on carbide formation in the carburized zone of M50NiL are of great importance because they can be used to control surface properties of bearings. A series of homogeneous alloys (with M50NiL as base composition) containing various levels of carbon in the range of 0.15% to 1.5% (in wt.%) and heat treated at temperatures between 650°C to 1100°C were selected for characterizations. Eleven samples were chosen for carbide characterization and chemical analysis and their identifications are listed in Table 1.Five different carbides consisting of M6C, M2C, M7C3 and M23C6 were found in all eleven samples examined as shown in Table 1. M6C carbides (with least carbon) were found to be the major carbide in low carbon alloys (<0.3% C) and their amounts decreased as the carbon content increased. In sample C (0.3% C), most particles (95%) encountered were M6C carbide with a particle sizes range between 0.05 to 0.25 um. The M6C carbide are enriched in both Mo and Fe and have a fee structure with lattice parameter a=1.105 nm (Figure 1).

Download Full-text