Hydrothermal green synthesis of gold nanoparticles using mushroom (Agaricus bisporus) extract: physico-chemical characteristics and antifungal activity studies

2018 ◽  
Vol 7 (1) ◽  
pp. 38-47 ◽  
Author(s):  
Maryam Eskandari-Nojedehi ◽  
Hoda Jafarizadeh-Malmiri ◽  
Javad Rahbar-Shahrouzi
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Antifungal Activity ◽  
Agaricus Bisporus ◽  
Hydrothermal Process ◽  
Edible Mushroom ◽  
Infrared Analysis ◽  
Stabilizing Agents ◽  
Mushroom Extract ◽  
Synthesis Parameters

AbstractEdible mushroom (Agaricus bisporus) extract was used to synthesize gold nanoparticles (AuNPs) through hydrothermal process (at a pressure of 15 psi and a temperature of 121°C for 15 min). Response surface methodology was applied to monitor the influence of the synthesis parameters, namely: the mushroom extract concentration (1–9 gr DP/100 ml distilled water) and the amount of HAuCL4·3H2O solution (8–12 ml) on the particle size and concentration of fabricated AuNPs. The obtained results demonstrated that while the main and quadratic terms of the synthesis parameters had significant (p<0.05) effects on the response variables, their interactions had insignificant effect on them. The results indicated that spherical synthesized AuNPs using 10 ml of HAuCl4·3H2O solution (1 mm) and 1 ml of mushroom extract with concentration of 5 gr DP/100 ml had mean particle size (25 nm) and maximum concentration (534 ppm) and stability (zeta potential of –45.8 mV). The results revealed that mushroom extract could act as both reducing and stabilizing agents due to its bioactive compounds. Fourier-transform infrared analysis showed that polyols and carbonyl groups in mushroom extract had strong effects on formation of stable AuNPs. The fabricated AuNPs exhibited high antifungal activity againstAspergillus flavusas compared to theAspergillus terreus.

Optimization of processing parameters in green synthesis of gold nanoparticles using microwave and edible mushroom (Agaricus bisporus) extract and evaluation of their antibacterial activity

2016 ◽  
Vol 5 (6) ◽  
Author(s):  
Maryam Eskandari-Nojehdehi ◽  
Hoda Jafarizadeh-Malmiri ◽  
Javad Rahbar-Shahrouzi
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Agaricus Bisporus ◽  
Edible Mushroom ◽  
Processing Parameters

AbstractGold nanoparticles (AuNPs) were synthesized using edible mushroom

scholarly journals Continuous citrate‐capped gold nanoparticle synthesis in a two‐phase flow reactor

2021 ◽  
Author(s):  
Spyridon Damilos ◽  
Ioannis Alissandratos ◽  
Luca Panariello ◽  
Anand N. P. Radhakrishnan ◽  
Enhong Cao ◽  
...  
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Residence Time ◽  
Flow Reactor ◽  
Phase Flow ◽  
Continuous Manufacturing ◽  
Two Phase ◽  
Process Yield ◽  
Au Nps ◽  
Manufacturing Platform

AbstractA continuous manufacturing platform was developed for the synthesis of aqueous colloidal 10–20 nm gold nanoparticles (Au NPs) in a flow reactor using chloroauric acid, sodium citrate and citric acid at 95 oC and 2.3 bar(a) pressure. The use of a two-phase flow system – using heptane as the continuous phase – prevented fouling on the reactor walls, while improving the residence time distribution. Continuous syntheses for up to 2 h demonstrated its potential application for continuous manufacturing, while live quality control was established using online UV-Vis photospectrometry that monitored the particle size and process yield. The synthesis was stable and reproducible over time for gold precursor concentration above 0.23 mM (after mixing), resulting in average particle size between 12 and 15 nm. A hydrophobic membrane separator provided successful separation of the aqueous and organic phases and collection of colloidal Au NPs in flow. Process yield increased at higher inlet flow rates (from 70 % to almost 100 %), due to lower residence time of the colloidal solution in the separator resulting in less fouling in the PTFE membrane. This study addresses the challenges for the translation of the synthesis from batch to flow and provides tools for the development of a continuous manufacturing platform for gold nanoparticles.Graphical abstract

In vitro permeation of gold nanoparticles through rat skin and rat intestine: Effect of particle size

2008 ◽  
Vol 65 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Ganeshchandra Sonavane ◽  
Keishiro Tomoda ◽  
Akira Sano ◽  
Hiroyuki Ohshima ◽  
Hiroshi Terada ◽  
...  
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Rat Skin ◽  
Rat Intestine ◽  
In Vitro Permeation ◽  
Effect Of Particle Size

Synthesis and Characterization of Water-Soluble Monolayer-Protected Gold Nanoparticles

2011 ◽  
Vol 415-417 ◽  
pp. 617-620 ◽  
Author(s):  
Yan Su ◽  
Ying Yun Lin ◽  
Yu Li Fu ◽  
Fan Qian ◽  
Xiu Pei Yang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Average Particle Size ◽  
Water Soluble ◽  
Synthesis And Characterization ◽  
Average Particle ◽  
Transmission Electron

Water-soluble gold nanoparticles (AuNPs) were prepared using 2-mercapto-4-methyl-5- thiazoleacetic acid (MMTA) as a stabilizing agent and sodium borohydride (NaBH4) as a reducing agent. The AuNPs product was analyzed by transmission electron microscopy (TEM), UV-vis absorption spectroscopy and Fourier transform infrared spectroscopy (FTIR). The TEM image shows that the particles were well-dispersed and their average particle size is about 5 nm. The UV-vis absorption and FTIR spectra confirm that the MMTA-AuNPs was stabilized by the carboxylate ions present on the surface of the AuNPs.

scholarly journals Effect of polyvalencies of glycotopes on the binding of a lectin from the edible mushroom, Agaricus bisporus

2003 ◽  
Vol 371 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Albert M. WU ◽  
June H. WU ◽  
Anthony HERP ◽  
Jia-Hau LIU
Keyword(s):  
Agaricus Bisporus ◽  
Therapeutic Potential ◽  
Specific Binding ◽  
Biological Activities ◽  
Edible Mushroom ◽  
Structural Requirement ◽  
Colon Cells ◽  
Lectin Receptor ◽  
Proliferative Effect ◽  
Potent Factor

Agaricus bisporus agglutinin (ABA) isolated from edible mushroom has a potent anti-proliferative effect on malignant colon cells with considerable therapeutic potential as an anti-neoplastic agent. Since previous studies on the structural requirement for binding were limited to molecular or submolecular levels of Galβ1-3GalNAc (T; Thomsen–Friedenreich disaccharide glycotope; where Gal represents d-galactopyranose and GalNAc represents 2-acetamido-2-deoxy-d-galactopyranose) and its derivatives, the binding properties of ABA were further investigated using our collection of glycans by enzyme-linked lectinosorbent assay and lectin–glycan inhibition assay. The results indicate that polyvalent Galβ1-related glycotopes, GalNAcα1-Ser/Thr (Tn), and their cryptoforms, are the most potent factor for ABA binding. They were up to 5.5×105 and 4.7×106 times more active than monomeric T and GalNAc respectively. The affinity of ABA for ligands can be ranked as: multivalent Tα (Galβ1-3GalNAcα1-), Tn and I/II (Galβ1-3GlcNac/Galβ1-4GlcNAc, where GlcNAc represents 2-acetamido-2-deoxy-d-glucopyranose)>>>>monomeric Tα and Tn>I>>GalNAc>>>II, L (Galβ1-4Glc, where Glc represents d-glucopyranose) and Gal (inactive). These specific binding features of ABA establish the importance of affinity enhancement by high-density polyvalent (versus multiantennary I/II) glycotopes and facilitate our understanding of the lectin receptor recognition events relevant to its biological activities.

Mushroom extract of Lactarius deliciosus (L.) Sf. Gray as biopesticide: Antifungal activity and toxicological analysis

2021 ◽  
pp. 1-13
Author(s):  
Lisiane Martins Volcão ◽  
Priscila Cristina Bartolomeu Halicki ◽  
Anelise Christ-Ribeiro ◽  
Daniela Fernandes Ramos ◽  
Eliana Badiale-Furlong ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Lactarius Deliciosus ◽  
Toxicological Analysis ◽  
Mushroom Extract

scholarly journals Biosynthesis and Potential Applications of Silver and Gold Nanoparticles and Their Chitosan-Based Nanocomposites in Nanomedicine

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Haliza Katas ◽  
Noor Zianah Moden ◽  
Chei Sin Lim ◽  
Terence Celesistinus ◽  
Jie Yee Chan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Metallic Nanoparticles ◽  
Cost Effective ◽  
One Pot ◽  
Silver And Gold Nanoparticles ◽  
Stabilizing Agents ◽  
Cost Effective Method ◽  
Multistep Process ◽  
Potential Applications ◽  
Biological Sources

Biosynthesized or biogenic metallic nanoparticles, particularly silver and gold nanoparticles (AgNPs and AuNPs, respectively), have been increasingly used because of their advantages, including high stability and loading capacity; moreover, these nanoparticles are synthesized using a green and cost-effective method. Previous studies have investigated reducing and/or stabilizing agents from various biological sources, including plants, microorganisms, and marine-derived products, using either a one-pot or a multistep process at different conditions. In addition, extensive studies have been performed to determine the biological or pharmacological effects of these nanoparticles, such as antimicrobial, antitumor, anti-inflammatory, and antioxidant effects. In the recent years, chitosan, a natural cationic polysaccharide, has been increasingly investigated as a reducing and/or stabilizing agent in the synthesis of biogenic metallic nanoparticles with potential applications in nanomedicine. Here, we have reviewed the mechanism of biosynthesis and potential applications of AgNPs and AuNPs and their chitosan-mediated nanocomposites in nanomedicine.

scholarly journals Protein-Coated Aryl Modified Gold Nanoparticles for Cellular Uptake Study by Osteosarcoma Cancer Cells

2020 ◽  
Author(s):  
Mehvesh Hameed ◽  
Seema Panicker ◽  
Sallam Hasan Abdallah ◽  
Amir A. Khan ◽  
Changseok Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Phase Transfer ◽  
Chemical Reduction ◽  
Uptake Study ◽  
Stabilizing Agents ◽  
Coated Nanoparticles ◽  
Green Route ◽  
The Difference

We synthesized protein-coated gold nanoparticles using green and chemical reduction routes for cellular uptake study. In the current work, we coated gold-aryl nanoparticles of the type AuNPs-C<sub>6</sub>H<sub>4</sub>-4-COOH with BSA, collagen, zein and lysozyme proteins. Both routes were carried out without phase-transfer catalysts or extraneous stabilizing agents. High crystallinity of the AuNPs synthesized by the green route can be seen in the transmission electron microscopy images. <a>Osteosarcoma cancer cells are malignant bone tumors with abnormal cellular functions. Studies using MG-63 cells will provide mechanistic suggestions on the details of the amplification in tumors. </a>We studied the cellular uptake of the bioconjugates by MG-63 osteosarcoma cells using laser confocal fluorescence microscopy (LCFM) and flow cytometry. In the LCFM study, BSA-AuNPs was uptaken most efficiently of all protein-coated gold nanoparticles synthesized by the green route. Zein and lysozyme coated nanoparticles, though small sizes, prepared by the green method were not efficiently uptaken by MG-63. The two nanoparticles are negatively charged and zein is also a hydrophobic coat. The difference in hydrophobicity and charge might have affected the internalization. All of those coated nanoparticles that were efficiently uptaken can potentially be used as diagnostic and therapeutic agents for osteosarcoma.

Sign in / Sign up

Export Citation Format

Share Document