Novel application of three-dimensional HDlive imaging in prenatal diagnosis from the first trimester

2015 ◽  
Vol 43 (2) ◽  
Author(s):  
Ritsuko Kimata Pooh ◽  
Asim Kurjak
Keyword(s):  
Human Development ◽  
New Technology ◽  
Three Dimensional ◽  
First Trimester ◽  
High Definition ◽  
Noninvasive Diagnostics ◽  
Anatomy And Physiology ◽  
Early Human Development ◽  
2D Ultrasound ◽  
Early Human

AbstractRecent development of three-dimensional (3D) high definition (HD) ultrasound has resulted in remarkable progress in visualization of early embryos and fetuses in sonoembryology. The new technology of HDlive assesses both structural and functional developments in the first trimester with greater reliably than two-dimensional (2D) ultrasound. The ability to visualize not only fetal face, hands, fingers, feet, and toes, but also amniotic membranes, is better with volumetric ultrasound than 2D ultrasound. In this article, detailed and comprehensive structures of normal and abnormal fetuses depicted by 3D HDlive are presented, including various faces of Down’s syndrome and holoprosencephaly, as well as low-set ear and finger/toe abnormalities from the first trimester. Three-dimensional HDlive further “humanizes” the fetus, enables detailed observation of the fetal face in the first trimester as shown in this article, and reveals that a small fetus is not more a fetus but a “person” from the first trimester. There has been an immense acceleration in understanding of early human development. The anatomy and physiology of embryonic development is a field where medicine exerts greatest impact on early pregnancy at present, and it opens fascinating aspects of embryonic differentiation. Clinical assessment of those stages of growth relies heavily on 3D/four-dimensional (4D) HDlive, one of the most promising forms of noninvasive diagnostics and embryological phenomena, once matters for textbooks are now routinely recorded with outstanding clarity. New advances deserve the adjective “breathtaking”, including 4D parallel study of the structural and functional early human development.

scholarly journals Consecutive appearance of coagulation factor XIII subunit A in macrophages, megakaryocytes, and liver cells during early human development

Blood ◽  
1995 ◽  
Vol 86 (6) ◽  
pp. 2191-2197 ◽  
Author(s):  
J Kappelmayer ◽  
G Bacsko ◽  
L Birinyi ◽  
R Zakany ◽  
E Kelemen ◽  
...  
Keyword(s):  
Human Development ◽  
Factor Xiii ◽  
Coagulation Factor ◽  
First Trimester ◽  
Liver Cells ◽  
Double Labeling ◽  
Subunit A ◽  
Early Human Development ◽  
Gestational Week ◽  
Early Human

Thirty embryonic and fetal samples were investigated to study the appearance and characteristics of factor XIII subunit A (FXIIIA)- containing cells in the course of human development. Samples were either vacuum-embedded in paraffin for staining FXIIIA by a sensitive biotin-streptavidin system or snap-frozen for double-labeling studies to characterize FXIIIA-containing cells. FXIIIA appeared as early as the fifth gestational week in yolk sac samples in stellate-shaped cells. Nonparenchymal cells in liver samples showed intense labeling for FXIIIA from the sixth week of gestation. The relative amount of FXIIIA-containing cells rapidly diminished up to the 13th gestational week. When characterized, the majority of these cells proved to be KiM7- positive macrophages, while GPIb (CD42b)-labeled cells accounted for less than 10% of FXIIIA-positive cells. Liver cells did not show any staining for FXIIIA in first trimester samples. The earliest liver specimen showing FXIIIA was at the 20th week, when FXIIIA appeared in some liver cells, particularly in those surrounding the central veins. In bone marrow smears, FXIIIA-positive cells started to appear at week 10 in the clavicles and increased in number in subsequent stages of development. Intracellular FXIIIA was distributed between GPIb-, RFD7-, and KiM7-positive cells. The results indicate that, apart from liver cells, at least three different cell populations (KiM7+ RFD7+ GPIb-, KiM7- RFD7- GPIb-, and KiM7- RFD7- GPIb+) contain FXIIIA in the early phase of human development. We conclude that FXIIIA appears very early during human development and is detectable in both extra- and intraembryonic hematopoietic organs. Intracellular FXIIIA in early human development is distributed between different macrophages and megakaryocytes, and by week 20, it appears in liver cells as well.

scholarly journals Advances in Visualization of the Early Human Development

2009 ◽  
Vol 3 (3) ◽  
pp. 25-38 ◽  
Author(s):  
Guillermo Azumendi ◽  
Kazuo Maeda ◽  
Ritsuko K Pooh ◽  
Iva Lausin
Keyword(s):  
Human Development ◽  
Human Life ◽  
Accurate Information ◽  
Two Dimensional ◽  
3 Dimensional ◽  
Anatomy And Physiology ◽  
Early Human Development ◽  
Early Embryos ◽  
First Time ◽  
Early Human

Abstract The anatomy and physiology of placental and embryonic development is a field where medicine exerts its impact on early pregnancy and opens fascinating aspects of embryonic differentiation. The introduction of high frequency transvaginal transducers as well as three and four dimensional sonography has resulted in remarkable progress in ultrasonic visualization of early embryos and fetuses. Ultrasound has been widely used in the field of early human development due to its safety, diagnostic accuracy and convenience. Normal fetal anatomy and development have been widely investigated using two-dimensional ultrasound and most of the knowledge regarding early human development were established through understanding of sectional images of fetal body and organs obtained by two-dimensional ultrasound. Usage of new techniques has produced more objective and accurate information of embryonal and early fetal development. For the first time parallel analyses of structural and functional parameters in the first 12 weeks of gestation become possible. This article deals with establishment of human life from ovum and sperm, though fertilization, detailed histological development and the establishment of the placenta, and early human development visualized by 2- and 3-dimensional ultrasonography.

Structural and functional early human development assessed by three-dimensional and four-dimensional sonography

2005 ◽  
Vol 84 (5) ◽  
pp. 1285-1299 ◽  
Author(s):  
Asim Kurjak ◽  
Ritsuko K. Pooh ◽  
Luis T. Merce ◽  
Jose M. Carrera ◽  
Aida Salihagic-Kadic ◽  
...  
Keyword(s):  
Human Development ◽  
Three Dimensional ◽  
Early Human Development ◽  
Early Human

scholarly journals Opening the black box: Stem cell–based modeling of human post-implantation development

2018 ◽  
Vol 218 (2) ◽  
pp. 410-421 ◽  
Author(s):  
Kenichiro Taniguchi ◽  
Idse Heemskerk ◽  
Deborah L. Gumucio
Keyword(s):  
Stem Cell ◽  
Human Development ◽  
Experimental Models ◽  
Uterine Wall ◽  
Black Box ◽  
Cellular Mechanisms ◽  
Early Human Development ◽  
Post Implantation ◽  
Early Human

Proper development of the human embryo following its implantation into the uterine wall is critical for the successful continuation of pregnancy. However, the complex cellular and molecular changes that occur during this post-implantation period of human development are not amenable to study in vivo. Recently, several new embryo-like human pluripotent stem cell (hPSC)–based platforms have emerged, which are beginning to illuminate the current black box state of early human post-implantation biology. In this review, we will discuss how these experimental models are carving a way for understanding novel molecular and cellular mechanisms during early human development.

scholarly journals Development of 3D Ultrasound

2010 ◽  
Vol 4 (3) ◽  
pp. 205-215 ◽  
Author(s):  
Kazunori Baba
Keyword(s):  
Human Development ◽  
Clinical Application ◽  
3D Ultrasound ◽  
Obstetrics And Gynecology ◽  
General Interest ◽  
Early Human Development ◽  
3D Sonography ◽  
Further Development ◽  
Early Human ◽  
Made In

Abstract Significant advances have been made in recent years in clinical application of 3D sonography in both obstetrics and gynecology. Since the author pioneered the use of 3D sonography many new clinical useful techniques have been used for better visualization of early human development and for the diagnostics of many gynecological problems as well as the use of 3D in management of female infertility. In this review we are describing further development in 3D ultrasound, which should be of general interest of readers of this journal.

Two- and three-dimensional sonography of anomalies of orofacial area in the first trimester of gestation

2020 ◽  
Author(s):  
N.A. Venchikova, À.À. Ershova-Pavlova, I.V. Novikova et all
Keyword(s):  
Pregnant Women ◽  
Three Dimensional ◽  
First Trimester ◽  
Population Based ◽  
Control Group ◽  
Axial Section ◽  
Mother And Child ◽  
Trimester Screening ◽  
2D Ultrasound ◽  
3D Sonography

As part of the population-based prenatal screening of 900 pregnant women, who underwent 1st trimester screening at the RPMC “Mother and Child” in 2018–2019, an ultrasound scan of the fetal palate was performed to evaluate the effectiveness of new ultrasound markers in detecting orofacial clefts (OFC). Fetal palatal defects were screened at a 2D assessment of bone markers of the palate – the image of the palatal line in the sagittal section, the retronasal triangle in the coronary section, the alveolar arch of the upper jaw in the axial section. If a palate abnormality was detected or suspected in the 2D study, the fetal palate was evaluated using the 3D mode. In 50 pregnant women of the control group, 3D sonography of the fetal palate was performed in the norm. In total, during the gestation period of 10+5–12+5 weeks, 10 cases of OFC were detected, of which 1 case was a median lip/palate cleft (LPC), 6 — bilateral LPC, 1 — left-sided LPC, 1 — unspecified LPC, 1 — PC. Visualization of 2D ultrasound markers was possible in 9 cases of palate anomalies, 3D assessment worked out in all 10 cases. In all identified OFC cases, cytogenetic and/or pathomorphological studies were performed.

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