scholarly journals Isolation and characterisation of peroxiredoxin 6 promoter from sheep (Ovis aries)

2016 ◽  
Vol 60 (3) ◽  
pp. 315-321 ◽  
Author(s):  
Nan-Nan Liu ◽  
Zeng-Shan Liu ◽  
Shi-Ying Lu ◽  
Pan Hu ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Gene Promoter ◽  
Flank Region ◽  
Ovis Aries ◽  
Biological Processes ◽  
Dna Fragments ◽  
Peroxiredoxin 6 ◽  
Cis Acting ◽  
Flanking Region ◽  
Basal Transcriptional Activity

AbstractIntroduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein and a unique 1-Cys Prdx of the peroxiredoxin family. The expression and regulation of Prdx6 are implicated in numerous physiological and pathological processes.Material and Methods: Eight stepwise truncated DNA fragments obtained from the 5′-flank region of the Prdx6 gene were prepared and subcloned into the pSEAP2-Enhancer vectors. To investigate the transcriptional activity of the truncated DNA fragments, the recombinant plasmids were transfected into the COS-1 cells and the transcriptional activity was measured via assaying the expression of the reporter gene of the secreted alkaline phosphatase.Results: A 3.4 kb 5′-upstream flank region of the Prdx6 gene was cloned and sequenced. The region from −108 nt to −36 nt of the 5′-flanking region of the Prdx6 gene contained basal transcriptional activity.Conclusion: This result provides the basis for further studies on the gene regulation of the Prdx6-mediated biological processes and on screening for the transacting factors that interact with cis-acting elements of the Prdx6 gene promoter.

scholarly journals A combination of closely associated positive and negative cis-acting promoter elements regulates transcription of the skeletal alpha-actin gene.

1990 ◽  
Vol 10 (2) ◽  
pp. 528-538 ◽  
Author(s):  
K L Chow ◽  
R J Schwartz
Keyword(s):  
Transcriptional Activity ◽  
Primary Cultures ◽  
Gene Promoter ◽  
Regulatory Sequence ◽  
Actin Gene ◽  
Cell Type ◽  
Specific Expression ◽  
Cis Acting ◽  
Cell Type Specific Expression ◽  
Alpha Actin

The chicken skeletal alpha-actin gene promoter region provides at least a 75-fold-greater transcriptional activity in muscle cells than in fibroblasts. The cis-acting sequences required for cell type-restricted expression within this 200-base-pair (bp) region were elucidated by chloramphenicol acetyltransferase assays of site-directed Bg/II linker-scanning mutations transiently transfected into primary cultures. Four positive cis-acting elements were identified and are required for efficient transcriptional activity in myogenic cells. These elements, conserved across vertebrate evolution, include the ATAAAA box (-24 bp), paired CCAAT-box-associated repeats (CBARs; at -83 bp and -127 bp), and the upstream T+A-rich regulatory sequence (at -176 bp). Basal transcriptional activity in fibroblasts was not as dependent on the upstream CBAR or regions of the upstream T+A-rich regulatory sequence. Transfection experiments provided evidence that positive regulatory factors required for alpha-actin expression in fibroblasts are limiting. In addition, negative cis-acting elements were detected and found closely associated with the G+C-rich sequences that surround the paired CBARs. Negative elements may have a role in restricting developmentally timed expression in myoblasts and appear to inhibit promoter activity in nonmyogenic cells. Cell type-specific expression of the skeletal alpha-actin gene promoter is regulated by combinatorial and possibly competitive interactions between multiple positive and negative cis-acting elements.

The Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Autographa californica Multicapsid Nucleopolyhedrovirus

2004 ◽  
Vol 59 (9-10) ◽  
pp. 749-754 ◽  
Author(s):  
Xing-Jia Shen ◽  
Yong-Zhu Yi ◽  
Shun-Ming Tang ◽  
Zhi-Fang Zhang ◽  
Yi-Ren Li ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Gene Promoter ◽  
Enzymatic Digestion ◽  
Initiation Site ◽  
Autographa Californica ◽  
Translation Initiation Site ◽  
Homologous Region ◽  
Luciferase Gene ◽  
Negative Effects ◽  
Cis Acting

Abstract The ecdysteroid UDP-glucosyltransferase (egt) gene promoter fragments of different lengths were generated from the genomic DNA of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) by PCR. After being purified and enzymatic digestion, they were cloned into the pGEM-3Z vector for construction of reporter plasmids pAcegt542- luc, pAcegt309-luc and pAcegt159-luc with the luciferase gene driven by the AcMNPV egt promoter. The results of transient expression in the Spodoptera frugiperda cell line-21 (Sf21) showed that the transcriptional activity of the AcMNPV egt promoter required the transactivation of viral factor(s). The expression of luciferase gene driven by the AcMNPV egt promoter was first detected at 24 h post infection. The egt promoter from the Bombyx mori nucleopolyhedrovirus (BmNPV), closely related to AcMNPV, revealed similar properties to that of the AcMNPV egt promoter. When BmNPV homologous region 3 was subcloned downstream the luciferase gene, the luciferase activity of the reporter plasmid was enhanced by over 1000-fold, but the property of the promoter was not changed. As a substrate of ecdysteroid UDP-glucosyltransferase (EGT), foreign insect ecdysone showed negative effects on egt promoter transcriptional activity. Ecdysone of 1.0-2.0 μg/ml significantly down-regulated the promoter activity. Promoter activities of different lengths showed that an AcMNPV egt promoter fragment of 159 bp has the basal transcriptional activity but it was almost abolished only about 0.2% of that of 309 bp and 542 bp, respectively, and the nucleotide sequence from - 159 to - 309 nt upstream the translation initiation site includes the main cis-acting elements interacting with viral factors.

scholarly journals Cloning and Characterization of β-Carotene Ketolase Gene Promoter in Haematococcus pluvialis

2005 ◽  
Vol 37 (4) ◽  
pp. 270-275 ◽  
Author(s):  
Chun-Xiao Meng ◽  
Chang-Ying Teng ◽  
Peng Jiang ◽  
Song Qin ◽  
Cheng-Kui Tseng
Keyword(s):  
Transcriptional Control ◽  
Haematococcus Pluvialis ◽  
Regulatory Element ◽  
Gene Promoter ◽  
Site Directed Mutagenesis ◽  
Cis Acting ◽  
Sterol Regulatory Element ◽  
Flanking Region ◽  
Responsive Element ◽  
Β Carotene

AbstractThe unicellular green alga Haematococcus pluvialis accumulates a highly valuable ketocarotenoid, astaxanthin, under various environmental stresses. β-carotene ketolase (BKT) plays a key role in astaxanthin biosynthesis in H. pluvialis. In this paper, an approximate 700 bp 5′-flanking region of the bkt gene containing a putative promoter was cloned through walking upstream. The results of the sequence analysis showed that this bkt 5′-flanking region might have cis-acting elements such as sterol regulatory element (SRE-1)-like motifs, the C-repeat/dehydration responsive element (DRE) and al-3 proximal element (APE)-like motifs, except for typical TATA and CCAAT boxes. The results of the β-galactosidase assay and the transient expression of lacZ driven by a series of sequential deletions revealed that a minimal promoter-like region might exist from -630 to -408 bp, and the highest promoter activity was observed to span the positions from -630 to -308 bp. The results of the site-directed mutagenesis of a C-repeat/DRE and two APE-like motifs in a promoter-like region (-630 to -308 bp) suggested that two APE-like motifs might be essential for transcriptional control of the bkt gene.

A combination of closely associated positive and negative cis-acting promoter elements regulates transcription of the skeletal alpha-actin gene

1990 ◽  
Vol 10 (2) ◽  
pp. 528-538
Author(s):  
K L Chow ◽  
R J Schwartz
Keyword(s):  
Transcriptional Activity ◽  
Primary Cultures ◽  
Gene Promoter ◽  
Regulatory Sequence ◽  
Actin Gene ◽  
Cell Type ◽  
Specific Expression ◽  
Cis Acting ◽  
Cell Type Specific Expression ◽  
Alpha Actin

The chicken skeletal alpha-actin gene promoter region provides at least a 75-fold-greater transcriptional activity in muscle cells than in fibroblasts. The cis-acting sequences required for cell type-restricted expression within this 200-base-pair (bp) region were elucidated by chloramphenicol acetyltransferase assays of site-directed Bg/II linker-scanning mutations transiently transfected into primary cultures. Four positive cis-acting elements were identified and are required for efficient transcriptional activity in myogenic cells. These elements, conserved across vertebrate evolution, include the ATAAAA box (-24 bp), paired CCAAT-box-associated repeats (CBARs; at -83 bp and -127 bp), and the upstream T+A-rich regulatory sequence (at -176 bp). Basal transcriptional activity in fibroblasts was not as dependent on the upstream CBAR or regions of the upstream T+A-rich regulatory sequence. Transfection experiments provided evidence that positive regulatory factors required for alpha-actin expression in fibroblasts are limiting. In addition, negative cis-acting elements were detected and found closely associated with the G+C-rich sequences that surround the paired CBARs. Negative elements may have a role in restricting developmentally timed expression in myoblasts and appear to inhibit promoter activity in nonmyogenic cells. Cell type-specific expression of the skeletal alpha-actin gene promoter is regulated by combinatorial and possibly competitive interactions between multiple positive and negative cis-acting elements.

Review of Progress in Predicting Protein Methylation Sites

2019 ◽  
Vol 23 (15) ◽  
pp. 1663-1670 ◽  
Author(s):  
Chunyan Ao ◽  
Shunshan Jin ◽  
Yuan Lin ◽  
Quan Zou
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Transcriptional Activity ◽  
Regulation Of Gene Expression ◽  
Protein Methylation ◽  
Biological Processes ◽  
Post Translational Modification ◽  
Regulatory Enzymes ◽  
Lysine Residues ◽  
Arginine Residues

Protein methylation is an important and reversible post-translational modification that regulates many biological processes in cells. It occurs mainly on lysine and arginine residues and involves many important biological processes, including transcriptional activity, signal transduction, and the regulation of gene expression. Protein methylation and its regulatory enzymes are related to a variety of human diseases, so improved identification of methylation sites is useful for designing drugs for a variety of related diseases. In this review, we systematically summarize and analyze the tools used for the prediction of protein methylation sites on arginine and lysine residues over the last decade.

Functional analysis of differences in transcriptional activity conferred by genetic variants in the 5′ flanking region of the IL12RB2 gene

2015 ◽  
Vol 68 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Nahoko Kato-Kogoe ◽  
Hideki Ohyama ◽  
Soichiro Okano ◽  
Koji Yamanegi ◽  
Naoko Yamada ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Genetic Variants ◽  
Transcriptional Activity ◽  
Flanking Region

scholarly journals Characterization of cis-acting elements in the rat ATP citrate-lyase gene promoter

2002 ◽  
Vol 34 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Young-An Moon ◽  
Sahng-Wook Park ◽  
Kyung-Sup Kim
Keyword(s):  
Gene Promoter ◽  
Atp Citrate Lyase ◽  
Cis Acting ◽  
Citrate Lyase

Replication-independent cis-acting element of a maize histone gene promoter

Plant Science ◽  
1993 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Marc Lepetit ◽  
Martine Ehling ◽  
Rossitza Atanassova ◽  
Nicole Chaubet ◽  
Claude Gigot
Keyword(s):  
Gene Promoter ◽  
Histone Gene ◽  
Cis Acting
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