scholarly journals Plasmonic nanocrystals on polycarbonate substrates for direct and label-free biodetection of Interleukin-6 in bioengineered 3D skeletal muscles

Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gerardo A Lopez-Muñoz ◽  
Juan M Fernández-Costa ◽  
Maria Alejandra Ortega ◽  
Jordina Balaguer-Trias ◽  
Eduard Martin-Lasierra ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Culture Media ◽  
Incident Angle ◽  
Wide Field ◽  
Label Free ◽  
Cell Culture Media ◽  
Muscle Tissues ◽  
Label Free Detection ◽  
Potential Benefits

Abstract The development of nanostructured plasmonic biosensors has been widely widespread in the last years, motivated by the potential benefits they can offer in integration, miniaturization, multiplexing opportunities, and enhanced performance label-free biodetection in a wide field of applications. Between them, engineering tissues represent a novel, challenging, and prolific application field for nanostructured plasmonic biosensors considering the previously described benefits and the low levels of secreted biomarkers (≈pM–nM) to detect. Here, we present an integrated plasmonic nanocrystals-based biosensor using high throughput nanostructured polycarbonate substrates. Metallic film thickness and incident angle of light for reflectance measurements were optimized to enhance the detection of antibody–antigen biorecognition events using numerical simulations. We achieved an enhancement in biodetection up to 3× as the incident angle of light decreases, which can be related to shorter evanescent decay lengths. We achieved a high reproducibility between channels with a coefficient of variation below 2% in bulk refractive index measurements, demonstrating a high potential for multiplexed sensing. Finally, biosensing potential was demonstrated by the direct and label-free detection of interleukin-6 biomarker in undiluted cell culture media supernatants from bioengineered 3D skeletal muscle tissues stimulated with different concentrations of endotoxins achieving a limit of detection (LOD) of ≈ 0.03 ng/mL (1.4 pM).

One-Dimensional Diffraction Grating of Poly(Methacrylic Acid) Brushes For The Rapid Label-Free Detection of Yersinia Pestis With a Reflective Laser System

2021 ◽  
Author(s):  
Feng-Ping Lin ◽  
Hui-Ling Hsu ◽  
Hui-Chung Lin ◽  
Hsin-Hsien Huang ◽  
Chien-Hsing Lu ◽  
...  
Keyword(s):  
Laser Beam ◽  
Yersinia Pestis ◽  
Methacrylic Acid ◽  
Limit Of Detection ◽  
Incident Angle ◽  
Diffraction Effect ◽  
Label Free ◽  
Diffraction Intensity ◽  
One Dimensional ◽  
Label Free Detection

Abstract Background: Because of the low sensitivity of commercial products, development of a facile method to rapidly identify plague on-site remains highly attractive. Line arrays of poly(methacrylic acid) (PMAA) brushes were grafted using a photoresist template to fabricate one-dimensional diffraction gratings (DGs). The as-prepared samples first bound protein G to immobilize and orient the tails of the antibody of Yersinia pestis (abY). A laser beam was employed to analyze the 2D and 3D reflective signals of DGs at an incident angle of 45°. The abY-tailed PMAA DG possessed an optical feature with a characteristic diffraction effect along the SII, in which the projection of the laser beam on the plane of the DG chip was parallel to the strips, and ST configurations, in which they were perpendicular. A fluidic diffraction chip based on the abY-tailed PMMA DG was fabricated to examine the ability to detect Yersinia pestis along the ST configuration. Results: Upon flowing through the chip, Yersinia pestis was attached to the abY-tailed PMMA DG, which changed the diffraction intensity. The degree of the diffraction intensity exhibited a linear response to Yersinia pestis at concentrations from 102 to 107 CFU mL−1, and the limit of detection was 75 CFU mL−1, 1000 times lower than a commercial product (Alexter Bio-Detect Test). The diffractive sensor could selectively detect Yersinia pestis in spiked serum samples, with excellent standard deviation and recovery. Conclusion: Our platform provides a simple, label-free method for on-site plague diagnosis to prevent the highly rapid transmission of plague.

scholarly journals Surface Plasmon Resonance Assay for Label-Free and Selective Detection of HIV-1 p24 Protein

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 180
Author(s):  
Lucia Sarcina ◽  
Giuseppe Felice Mangiatordi ◽  
Fabrizio Torricelli ◽  
Paolo Bollella ◽  
Zahra Gounani ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Covalent Binding ◽  
Hiv Infections ◽  
Selectivity Ratio ◽  
Selective Detection ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Label Free Detection ◽  
P24 Protein ◽  
Hiv 1

The early detection of the human immunodeficiency virus (HIV) is of paramount importance to achieve efficient therapeutic treatment and limit the disease spreading. In this perspective, the assessment of biosensing assay for the HIV-1 p24 capsid protein plays a pivotal role in the timely and selective detection of HIV infections. In this study, multi-parameter-SPR has been used to develop a reliable and label-free detection method for HIV-1 p24 protein. Remarkably, both physical and chemical immobilization of mouse monoclonal antibodies against HIV-1 p24 on the SPR gold detecting surface have been characterized for the first time. The two immobilization techniques returned a capturing antibody surface coverage as high as (7.5 ± 0.3) × 1011 molecule/cm2 and (2.4 ± 0.6) × 1011 molecule/cm2, respectively. However, the covalent binding of the capturing antibodies through a mixed self-assembled monolayer (SAM) of alkanethiols led to a doubling of the p24 binding signal. Moreover, from the modeling of the dose-response curve, an equilibrium dissociation constant KD of 5.30 × 10−9 M was computed for the assay performed on the SAM modified surface compared to a much larger KD of 7.46 × 10−5 M extracted for the physisorbed antibodies. The chemically modified system was also characterized in terms of sensitivity and selectivity, reaching a limit of detection of (4.1 ± 0.5) nM and an unprecedented selectivity ratio of 0.02.

scholarly journals Rapid label-free SERS detection of foodborne pathogenic bacteria based on hafnium ditelluride-Au nanocomposites

2020 ◽  
Vol 13 (05) ◽  
pp. 2041004 ◽  
Author(s):  
Yang Li ◽  
Yanxian Guo ◽  
Binggang Ye ◽  
Zhengfei Zhuang ◽  
Peilin Lan ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Limit Of Detection ◽  
Principal Component ◽  
High Sensitivity ◽  
Chemical Mechanism ◽  
Sers Substrate ◽  
Label Free ◽  
Label Free Detection ◽  
Surface Enhanced Raman ◽  
Foodborne Pathogenic Bacteria

Two-dimensional (2D) nanomaterials have captured an increasing attention in biophotonics owing to their excellent optical features. Herein, 2D hafnium ditelluride (HfTe[Formula: see text], a new member of transition metal tellurides, is exploited to support gold nanoparticles fabricating HfTe2-Au nanocomposites. The nanohybrids can serve as novel 2D surface-enhanced Raman scattering (SERS) substrate for the label-free detection of analyte with high sensitivity and reproducibility. Chemical mechanism originated from HfTe2 nanosheets and the electromagnetic enhancement induced by the hot spots on the nanohybrids may largely contribute to the superior SERS effect of HfTe2-Au nanocomposites. Finally, HfTe2-Au nanocomposites are utilized for the label-free SERS analysis of foodborne pathogenic bacteria, which realize the rapid and ultrasensitive Raman test of Escherichia coli, Listeria monocytogenes, Staphylococcus aureus and Salmonella with the limit of detection of 10 CFU/mL and the maximum Raman enhancement factor up to [Formula: see text]. Combined with principal component analysis, HfTe2-Au-based SERS analysis also completes the bacterial classification without extra treatment.

scholarly journals Acoustic Immunosensing of Exosomes Using a Quartz Crystal Microbalance with Dissipation Monitoring

2019 ◽  
Author(s):  
Jugal Suthar ◽  
Edward Parsons ◽  
Bart Hoogenboom ◽  
Gareth Williams ◽  
Stefan Guldin
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Lipid Membrane ◽  
Acoustic Analysis ◽  
Limit Of Detection ◽  
Culture Media ◽  
Transmembrane Protein ◽  
Protein Profile ◽  
Size Exclusion ◽  
Label Free

Exosomes are endocytic lipid-membrane bound bodies with potential to be used as biomarkers in cancer and neurodegenerative disease. The limitations and scarcity of current exosome characterisation approaches has led to a growing demand for translational techniques, capable of determining their molecular composition and physical properties in physiological fluids. Here, we investigate label-free immunosensing, using a quartz crystal microbalance with dissipation (QCM-D), to detect exosomes by exploiting their surface protein profile. Exosomes expressing the transmembrane protein CD63 were isolated by size-exclusion chromatography from cell culture media. QCM-D sensors functionalised with anti-CD63 antibodies formed a direct immunoassay towards CD63-positive exosomes, exhibiting a limit-of-detection of 1.7x10^8 and 1.1x10^8 exosome sized particles (ESPs) ml^-1 for frequency and dissipation response respectively, i.e., clinically relevant concentrations. Our proof-of-concept findings support the adoption of dual-mode acoustic analysis of exosomes, leveraging both frequency and dissipation monitoring for use in diagnostic assays.

scholarly journals Wide-field Surface-Enhanced Coherent Anti-Stokes Raman Scattering Microscopy

2021 ◽  
Author(s):  
Cheng Zong ◽  
Ran Cheng ◽  
Fukai Chen ◽  
Peng Lin ◽  
Meng Zhang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Large Scale ◽  
Time Lapse ◽  
Live Cells ◽  
Large Field ◽  
Wide Field ◽  
Label Free ◽  
Label Free Detection ◽  
Surface Enhanced ◽  
Anti Stokes

Surface-enhanced Raman scattering (SERS) spectroscopy has been used extensively to study biology, chemistry, and materials. However, a point-by-point SERS mapping is time-consuming, taking minutes to hours for large-scale imaging. Here, we report a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscopy for monitoring nanotags in live cells and label-free detection of metabolic molecules. The WISE-CARS microscope achieves an imaging speed as fast as 120 frames per second for a large field of view of 130 microns X 130 microns. By spectral focusing of femtosecond lasers, a hyperspectral WISE-CARS stack of 120 frames can be acquired with a spectral resolution of 10 cm-1, where over 1 million Raman spectra are parallelly recorded within 0.5 seconds. As applications, we demonstrate time-lapse, 3D WISE-CARS imaging of nanotags in live cells as well as label-free detection of adenine released from S. aureus.

Nanophotonics based label free detection mechanism for real-time monitoring of interleukin-6

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9194-9207 ◽  
Author(s):  
Munezza A. Khan ◽  
Mohammad Mujahid ◽  
Say Chye Joachim Loo ◽  
Vidya N. Chamundeswari
Keyword(s):  
Photonic Crystals ◽  
Real Time ◽  
Interleukin 6 ◽  
High Fidelity ◽  
Label Free ◽  
Real Time Monitoring ◽  
Detection Mechanism ◽  
Label Free Detection ◽  
Real Time Detection

Magneto-photonic crystals/MPCs are promising candidates for devising high-fidelity embedded biosensor systems which offer facile & real time detection of diagnostic proteins.

A carbon ink screen-printed immunoelectrode for Dengue virus NS1protein detection based on photosynthesized amine gold nanoparticles

2018 ◽  
pp. 1-12
Keyword(s):  
Gold Nanoparticles ◽  
Dengue Virus ◽  
Limit Of Detection ◽  
Colloidal Suspension ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Vis Spectroscopy ◽  
Label Free Detection ◽  
The Impact ◽  
Screen Printed

Dengue virus (DENV) is a reemerging mosquito-borne disease that is endemic in more than 125 countries, affecting 200 million people per year. Screening testing has been a good attempt to minimize the impact caused by high morbity and mortality rates of DENV. In this study, a simple and disposable label-free electrochemical immunosensor based on a carbon ink graphite screen-printed electrode (SPE) one-step fabricated was developed for detection of non-structural 1 protein (NS1). The SPE surface was modified by drop casting, depositing a colloidal suspension containing amine-functionalized gold nanoparticles (AuNP-NH2). AuNPs were synthetized by a photoinduced physical method, illuminating preformed gold seeds with a light-emitting diode (LED,) at blue region, by using the polyethyleneimine (NH2) as reductor and stabilizing agent. UV-VIS spectroscopy and Transmission Electron Microscopy (TEM) were used to characterize the amine AuNPs. Electrocatalytic activity of AuNPs allowed more sensitivity for a label-free detection of NS1 by square wave voltammetry (SWV), with linear response from 0.1 to 2 µg mL-1. It was found a good linearity (coefficient of correlation of 0.995 (p<0.01) and a limit of detection of 0.03 µg mL-1 NS1 for analytical responses. AuNP-NH2 synthesis provided an easy oriented immobilization of anti-NS1 antibodies by Fc portion, resulting in a simple fabrication immunosensor with relative high performance and feasibility for early diagnostic of DENV.

scholarly journals Detecting Phase Shifts in Surface Plasmon Resonance: A Review

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Y. H. Huang ◽  
H. P. Ho ◽  
S. Y. Wu ◽  
S. K. Kong
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Biological Species ◽  
Optical Beam ◽  
Phase Detection ◽  
Label Free ◽  
Advantages And Disadvantages ◽  
Label Free Detection

Under certain conditions, a surface plasmon wave along a metal-dielectric interface can be excited by an optical beam. The reflected optical beam will then undergo changes in both intensity and phase. As the level of intensity or phase change is quite sensitive to the coupling conditions such as the molecule concentration on the metal surface, this phenomenon has been utilized for label-free detection of biological species and characterization of molecular interactions during the last two decades. Currently, most of the commercial surface plasmon resonance (SPR) sensors rely on the detection of absorption dip in angular or wavelength spectrum. However, recent researches have shown that phase detection has the potential to achieve lower limit of detection (LoD) and higher throughput. This paper, thus, intends to review various schemes and configurations for SPR phase detection. The performance advantages and disadvantages of various schemes will be emphasized. It is hoped that this paper will provide some insights to researchers interested in SPR sensing and help them to develop SPR sensors with better sensitivity and higher throughput.

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