scholarly journals The Application of Phosphate Solubilizing Microbes Biofertilizer to Increase Soil P and Yield of Maize on Ultisols Jatinangor

2017 ◽  
Vol 2 (6) ◽  
pp. 179
Author(s):  
Betty Natalie Fitriatin ◽  
Pujawati Suryatmana ◽  
Anny Yuniarti ◽  
Noor Istifadah

Ultisols has problems of low availability of nutrients, especially phosphorus. To improve soil phosphate and P fertilizer efficiency, it is necessary to develop biofertilizer such as phosphate solubilizing microbes. Phosphate solubilizing microbes (PSM) have the capability of dissolving soil phosphorus which have been adsorbed and can mineralize organic P to become inorganic P, hence increasing the avalibility of P in the soil. Phosphate solubilizing bacteria (Pseudomonas mallei and Pseudomonas cepacea) and phosphate solubilizing fungi (Penicillium sp. and Aspergillus sp) were selected based on their ability to dissolve P. The experiment was conducted at Jatinangor, West Java Indonesia to study the application of PSM biofertilizer to increase soil P and yield of maize. Experiment used a Randomized Block Design (RBD) in factorial pattern, consisting of two factors with three replications. The first factor consisted of PSM biofertilizer, which were; without PSM, 5 L ha-1 of PSM and 50 kg ha-1 of PSM.  The second factor was P fertilizer with five levels (0%, 25%, 50%, 75% and 100% dosage of recommendation). The results showed that the application of PSM biofertilizer increased soil phosphate and yield of maize on Ultisol Jatinangor.  The dosage of P inorganic fertilizers was reduced by 50%.Keywords: ultisol, maize, biofertillizer, phospate-solubilizing bacteria.

SoilREns ◽  
2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Betty Natalie Fitriatin ◽  
Aristyo Rahardiyan ◽  
Tien Turmuktini

Marginal soils have problems of low availability of nutrients, especially phosphorus. Phosphate solubilizing microbes can produce organic acid that dissolve P in the soil. Futhermore, the PSM also produce extracellular enzymes asa phosphatase that catalyze mineralization of organic P become inorganic P. Phosphate solubilizing bacteria (Pseudomonas mallei and Pseudomonas cepacea) and phosphate solubilizing fungi (Penicillium sp. and Aspergillus sp) is selected based on the ability of dissolving P and the production of growth regulators. Research at this stage aims to determine the biological fertilizer application techniques (PSB and PSF) as well as different types of P fertilizers to improve soil P available, growth and yield of maize. Field experiment on Ultisol Jatinangor implemented using a randomized block design (RBD) to test how applications PSM plus (giving 1, 2 and 3 applications) as well as the type of fertilizer P (SP-36 and rock phosphate). The results showed that the application of PSM can improve soil P-available growth and yield of maize. Application of PSM biological fertilizer could reduce the need for fertilizer P by 50%. PSM application once the application (early planting) with SP-36 dose 50% can increase content of soil P (P-available), and yield of maize on Ultisols.Keywords : maize, phosphate solubilizing microbes, Ultisols


2020 ◽  
Vol 2 (02) ◽  
pp. 74-81
Author(s):  
Fiqriah Hanum Khumairah ◽  
Anggi Jingga ◽  
Betty Natalie Fitriatin ◽  
Tualar Simarmata

Maize (Zea mays L.) is an important food crop in Indonesia as a source of carbohydrates. The extent of land conversion reduces maize productivity. The experiment aimed to increase the growth and yield of maize in Ultisol which was carried out in the experimental garden of the West Java Horticultural and Various Seed Plant Development Center from October 2017 to March 2018. The Phosphate Solubilizing Bacteria (PSB) and organic ameliorant application test used the factorial randomized block design (RBD) method consisted of two factors. The first factor is PSB and organic ameliorant which consisted of four levels, namely b0 (without the provision of PSB and organic ameliorant), b1 (BP 107 cfu mL-1), b2 (organic ameliorant 5 tons ha-1), and b3 (PSB 107 cfu mL-1 + organic ameliorant 5 tons ha-1) and the second factor is the dosage of SP-36 fertilizer consisted of five levels, namely po (without SP-36 fertilizer), p1 (100% SP-36 fertilizer), p2 (90% SP-36 fertilizer, P3 (80% SP-36 fertilizer), and P4 (70% SP-36 fertilizer). The results showed that the application of PSB (Enterobacter ludwigii + Bulkholderia vietnamiensis + Citrobacter amalonaticus) 107 cfu mL-1 and organic ameliorant dosage of 5 tons ha-1 was able to increase plant height at age 6 Week After Planting (WAP), weight of cob with and without cornhusk, and weight of 100 seeds in the framework of increasing the growth and yield of maize on Ultisols and reduce the use of inorganic P fertilizer to 90%.


2020 ◽  
Vol 5 (1) ◽  
pp. 300-304
Author(s):  
Betty Natalie Fitriatin ◽  
Dita Fauziah ◽  
Fabira Nur Fitriani ◽  
Dewi Nurma Ningtyas ◽  
Pujawati Suryatmana ◽  
...  

AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.


2013 ◽  
Vol 22 (2) ◽  
pp. 139
Author(s):  
L Izhar ◽  
A D Susila ◽  
B S Puswoko ◽  
A Sutandi ◽  
I W Mangku

ABSTRAK.  Fosfor merupakan salah satu hara penting tanah dan aplikasi hara tersebut pada konsentrasi yang sesuai sangat berpengaruh terhadap pertumbuhan tomat. Penelitian tentang studi analisis fosfor tanah dan aplikasi pupuk fosfor pada budidaya tomat pada tanah Inceptisols dilakukan di Kebun Percobaan dan Rumah Kaca di Cikabayan, Institut Pertanian Bogor, dari bulan Maret sampai dengan November 2010. Tujuan penelitian ialah untuk mendapatkan  metode  ekstraksi  fosfor tanah yang terbaik guna menentukan dosis pupuk fosfor pada budidaya tomat  pada tanah Inceptisols. Penelitian menggunakan rancangan acak kelompok dengan perlakuan pemberian pupuk fosfor pada beberapa tingkat dosis yaitu  0X, ¼ X, ½ X, ¾ X, dan 1X, di mana nilai X ialah 368,5 kg/ha P2O5 dengan empat ulangan. Perlakuan pemupukan fosfor diterapkan pada 6 bulan sebelum penanaman tomat. Analisis korelasi dilakukan antara kandungan P tanah dan pertumbuhan tanaman yang ditanam di dalam rumah kaca menggunakan media inkubasi berasal dari tanah yang diberi perlakuan dan dianalisis. Uji  fosfor  tanah menggunakan lima metode ekstraksi, yaitu metode Bray I (NH4F 0,03 N + HCl 0,025 N, nisbah 1:7); Bray II (NH4F 0,03 N + HCl 0,10 N ); Mehlich I (HCl 0,05 N + H2SO4 0,025 N); Morgan Wolf (NaC2H2H3O2.3H2O; pH 4,8); dan Truogh [HCl 0,10 N + (NH4)2SO4; pH 3]. Hasil penelitian menunjukkan adanya perbedaan yang nyata antara pengaruh perlakuan pupuk P terhadap parameter  tinggi tanaman,  jumlah daun, dan diameter batang tomat. Bobot segar biomassa dan bobot kering tomat juga menunjukkan  perbedaan pengaruh yang signifikan antarperlakuan. Nilai korelasi terbaik ditunjukkan oleh metode pengekstrak Mehlich I melalui parameter bobot kering dan bobot basah relatif tanaman. Dengan demikian, metode uji P tanah yang menggunakan Mehlich I dapat digunakan sebagai metode ekstraksi yang paling tepat untuk menganalisis unsur hara fosfor dengan koefisien korelasi 0,88, sehingga metode Mehlich I dapat diusulkan sebagai rekomendasi pemupukan P pada budidaya tomat pada tanah Inceptisols (nilai r = 0,89).  <br /><br />ABSTRACT. Izhar, L, Susila, AD, Purwoko, BS, Sutandi, A,  and  Mangku, IW. 2012. Determination of the Best Method of Soil P Test for Tomato (Lycopersicon esculentum Mill. L) on Inceptisols Soil. Phosphorus is one of important soil elements and application of the element in suitable concentration give high effect on tomato growth. A study on phosphorus analysis and its application for recommendation of soil fertilization of tomato cultivation on Inceptisols soil was conducted at the field and Greenhouse of Cikabayan, Bogor Agricultural University, from March to November 2010. The objective of this research was to obtain the best extraction method of soil-P test for determining phosphorus nutrient required for tomato cultivation on Inceptisol soil. Rate of phosphorus of 0X, ¼ X, ½ X, ¾ X, and 1X, where X was 368.5 kg/ha P2O5 with four replications, was applied in the study. The treatments were applied 6 months before planting date. The research was arranged in randomized complete block design. Analysis of correlation between soil-P and plant growth based on data collected from the plants grown in the greenhouse using incubation media in treated-soil was  analyzed. Soil-P test was carried out by using five extraction methods i.e. Bray I (HCl 0,025 N + NH4F 0.03), Bray II (NH4F 0.03 N + HCl 0.10 N), Mehlich I (HCl 0.05 N + H2SO4 0.025 N), Morgan Wolf (NaC2H2H3O2.3H2O; pH 4.8), and Truogh [HCl 0.10 N + (NH4)2SO4; pH 3]. The results showed that there were significant differences among the treatments of P fertilizer on the variables of plant height, leaf number, and stem diameter of tomato. Biomass fresh and dry weight of tomato also showed significantly different between the treatments applied. The highest correlation was shown on Mehlich I extraction reagent between plant dry and fresh weight. It means that, this P-nutrient extraction method was the most appropriate in determining phosphorus nutrient for tomatoes on Inceptisols soil with a coefficient correlation of 0.88. Mehlich I can also be used to develop a comprehensive phosphorus fertilizer recommendation for tomato cultivation on Inceptisols soil (r value = 0.89).<br /><br />


Agrologia ◽  
2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Betty Natalie Fitriatin ◽  
Mayang Agustina ◽  
Reginawanti Hindersah

Ultisol is low fertility soil and as low available P due to P fixation by Al and Fe. The phosphate solubilizing microbes (PSM) is the microbial group that enable realease fixed-P to soil solution and be absorbed by plant. This research was aimed to determine the effects of PSM biofertilizer and phosphate fertilizer dosage combination on soil potential P, total phosphate solubilizing bacteria (PSB) population and yield of maize (Zea mays L.) grown in Ultisols. This research used a Randomized Block Design that consist of nine treatments with three replications. The treatments were 100 kg ha-1 of SP-36 fertilizer, 50 kg ha-1 of PSM biofertilizer, SP-36 fertilizer (50 kg ha-1 and 100 kg ha-1) with PSM biofertilizer (25 kg ha-1, 50 kg ha-1 and 75 kg ha-1) repectively. The result showed that the combination of PSM and phosphate fertilizer dosage had significantly effect on PSB total population and maize yield, but did not not on  potential P. This experiment suggested that SP-36 fertilizer with 50 kg ha-1 dosage and PSM biofertilizer with 50 kg ha-1 dosage is the best combination.


2021 ◽  
Vol 8 (2) ◽  
pp. 437-449
Author(s):  
Gertruda Lovitna ◽  
Yulia Nuraini ◽  
Nurul Istiqomah

Fulfilment of nutrients for plants can be maximized when fertilization, but the application of inorganic fertilizers can cause damage to the soil if the dosage is excessive. The utilization of phosphate solubilizing bacteria is an effort to increase the efficiency of phosphate fertilization that can extract phosphate from an insoluble form that becomes available. This study aimed to obtain a combination of inorganic fertilization doses (SP-36 and NPK) and the application of biological fertilizers that utilize phosphate solubilizing bacteria (PSB) to increase the population of phosphate solubilizing bacteria and P-available in the soil, as well as corn crop yields. This study used a randomized block design with nine treatments and three replications. The result showed that the application of PSB and inorganic phosphate fertilizer shows a significant effect on the population of PSB, P-available, plant height, stover dry weight, cob weight with husk and cob weight without husk, but didn’t show a significant effect on 100 seeds weight and number of plant leaves. Based on the correlation analysis, the results show a positive correlation between soil chemical properties, P-available, and corn yield.


SoilREns ◽  
2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Betty Natalie Fitriatin ◽  
Muhammad Agi Pratama ◽  
Oviyanti Mulyani

Ultisols is a soil order with low available P. Phosphate Solubilizing Microbes is a group of microorganisms that has the ability to release P for plants. The aim of this experiment was to determine effect of phosphate solubilizing microbes and P fertilizer on soil aggregate stability, PSF population and yield of maize (Zea mays L.) on Ultisols. This research was conducted from May 2016 to August 2016 at Ciparanje, Faculty of Agriculture, Universitas Padjadjaran. This research was arranged in Randomized Block Design (RBD), consisted of 9 treatments with 3 replications. The treatments were 100 kg ha-1 of SP-36 fertilizer, 50 kg ha-1 of biofertilizer, as well as combination of SP-36 fertilizer (50% and 100%) with the dose of biofertilizer (50%, 100% and 150%). This experiment showed that the combination of biofertilizer and P fertilizer gave significant effect on the population of PSF and yield of maize, but not for stability of soil aggregates. The application of SP-36 50 kg ha-1 and 50 kg ha-1 PSM combination gave higher population of PSF and yield of maize.Keywords:  Biofertilizer, maize, phosphate- solubizing fungi, aggregate stability, Ultisols


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
BHALSHANKAR CHHAYA

Vermicompostis is a method of making compost with the use of earthworms. Neem cake is a seed residue left after extraction of oil used as a biopesticide and organic nitrogen fertilizer. Azotobacter bacteria are used as nitrogen fixing biofertilizer and Phosphate solubilizing bacteria. All these organic fertilizers and biofertilizers are managed to study their effect on yield of crop Vignaunguiculata. Treatments are given as VC, NC, Bio,NPK and control in randomized block design. Highest DM yield was shown inVC followed by NPK, BIO, and NC and lowest in CON.


Author(s):  
Amit Kumar ◽  
Mukesh Kumar ◽  
Bijendra Singh ◽  
Manoj Kumar Singh ◽  
S. P. Singh ◽  
...  

A field experiment was carried out at Horticulture Research Center of Sardar Vallabhbhai Patel University of Agriculture & Technology Meerut, (UP) India during rabi season 2018-19 and 2019-20 respectively with entitled “Effect of different sources of nutrients and mulching on sustainable production of Cauliflower (Brassica oleracea var. botrytis L.) cv. Pusa Ashwani”. The experiment was laid out in randomized block design with three replications and eleven treatments. The results showed that plants treated with integrated application of inorganic fertilizer, Vermicompost, Azotobacter, PSB (Phosphate Solubilizing Bacteria), Black mulch (2.5 mm) proved significantly better over the recommended practice 100% RDF (Recommended doses of fertilizer) and control. However, plants treated without organic manures and inorganic fertilizers resulted in significant reduction for various growth, yield and quality parameters. Among the treatments, plants fortified with application of 50% RDF + 15 t/ha Vermicompost + Azotobacter (5 kg/ha) + PSB (5kg/ha) + Black mulch (2.5 mm) was found to better growth, yield and quality for both years.


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