An evaluation of the usefulness of invasive and non-invasive methods used to diagnose Helicobacter spp. infections in dogs

2017 ◽  
Vol 20 (3) ◽  
pp. 491-499 ◽  
Author(s):  
M. Jankowski ◽  
J. Spużak ◽  
K. Kubiak ◽  
K. Glińska-Suchocka ◽  
M. Biernat
Keyword(s):  
Nested Pcr ◽  
Gastric Biopsy ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
Pcr Assay ◽  
Non Invasive ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Invasive Method ◽  
Pcr Method

AbstractThe aim of this study was to assess the suitability of invasive and non-invasive methods used to diagnose Helicobacter spp. in the stomachs of dogs. The study was carried out on 30 dogs of both sexes and different breeds, between one and 15 years old. A histopathologic examination, a microbiological culture, a rapid urease test, a direct bacteriological preparation and a nested PCR assay were carried out. Gastric Helicobacter spp. was identified in gastric biopsy specimens from 16 (53.3%) dogs using direct bacteriological preparation, in four (13.3%) dogs based on a culture, in 23 (76.6%) dogs using the rapid urease test and in 21 (70,0%) dogs based on a histopathological assessment of the biopsy specimens. The nested PCR of the gastric biopsy specimens revealed gastric Helicobacter spp. in all the dogs (100%). A saliva PCR assay revealed gastric Helicobacter spp. in 23 (76.6%) dogs, while stool PCR revealed the bacterium in seven (23.3%) dogs. We found that invasive methods were more accurate than non-invasive methods in detecting a Helicobacter spp. infection in dogs. In addition, the nested PCR method used to evaluate the gastric mucosal biopsy specimens was the most accurate test for detecting Helicobacter spp. It was further found that the PCR-based saliva assay was the best non-invasive method for detecting Helicobacter spp. However, taking into consideration that most of the diagnostic methods used to detect this bacterium have drawbacks, at least two diagnostic methods should be used to detect Helicobacter spp. as is done in human medicine.

scholarly journals Detection of “Candidatus Helicobacter suis” in Gastric Samples of Pigs by PCR: Comparison with Other Invasive Diagnostic Techniques

2000 ◽  
Vol 38 (3) ◽  
pp. 1131-1135 ◽  
Author(s):  
D. De Groote ◽  
R. Ducatelle ◽  
L. J. van Doorn ◽  
K. Tilmant ◽  
A. Verschuuren ◽  
...  
Keyword(s):  
Southern Blot ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Epidemiological Studies ◽  
Diagnostic Techniques ◽  
Diagnostic Methods ◽  
Giemsa Staining ◽  
Rapid Urease Test ◽  
Pcr Assay ◽  
Urease Test

Recently, a new 16S ribosomal DNA-based PCR assay was developed for the specific detection of “Candidatus Helicobacter suis” (former “Gastrospirillum suis”) in porcine gastric samples. In the present study, this PCR assay was compared to three other invasive diagnostic methods (rapid urease test, immunohistochemistry, histologic analysis by Giemsa staining). Antral stomach samples from 200 slaughterhouse pigs from Belgium and The Netherlands were examined. Bacterial presence was determined in 77% (154 of 200) of the samples by PCR in combination with Southern blot hybridization, 56% (111 of 200) of the samples by immunohistochemistry, 61% (122 of 200) of the samples by urease testing (20 h postinoculation [p.i.]), 36% (71 of 200) of the samples by urease testing (3 h p.i.), and 33% (65 of 200) of the samples by Giemsa staining. The intrinsic specificity of the PCR assay was assessed by Southern blot analysis with an “Candidatus H. suis”-specific probe and sequencing of PCR products. Interassay sensitivity and specificity values were assessed for each test by pairwise comparisons between tests. Agreement between tests was evaluated by calculating Cohen's kappa coefficient. From that analysis, the PCR assay was considered the most reliable benchmark. Microscopic detection of immunohistochemically labeled or Giemsa-stained “Candidatus H. suis” cells in stomach sections proved to be highly specific (100%) but relatively insensitive (72 and 42%, respectively) compared to the PCR assay. A longer incubation time of the urease test improved its sensitivity considerably (74 versus 55%) but was accompanied by a loss of specificity (72 versus 93%). In conclusion, we found the “Candidatus H. suis”-specific PCR assay to be a sensitive and reliable diagnostic method for the detection of “Candidatus H. suis” in the stomachs of pigs and could prove to be a valuable tool for further epidemiological studies both for “Candidatus H. suis”- and for “Helicobacter heilmannii” type 1-related research.

scholarly journals Helicobacter pyloriIs Not Eradicated after Triple Therapy: A Nested PCR Based Study

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Saurabh Kumar Patel ◽  
Girish Narayan Mishra ◽  
Chandra Bhan Pratap ◽  
Ashok Kumar Jain ◽  
Gopal Nath
Keyword(s):  
Triple Therapy ◽  
Nested Pcr ◽  
Restriction Pattern ◽  
Rapid Urease Test ◽  
Serological Tests ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Before And After ◽  
H Pylori ◽  
Antral Biopsy

Detection ofHelicobacter pyloriafter triple therapy is usually carried out by either rapid urease test (RUT), urea breath test (UBT), histology, bacterial isolation, and single round PCR or serological tests. In this study, antral biopsy specimens from 25 patients were tested forH. pyloriby RUT, culture, histology, and nested PCR in their antral biopsy specimens before and after treatment. Three genes, namely, heat shock protein (hsp60), phosphoglucosamine mutase (ureC), and flagellar export ATP synthase (fliI) ofH. pyloriwere targeted. Of the 25 antral biopsy specimens, the RUT, culture, histology, and nested PCR positivity dropped from 81.8% to 12%, 31% to 0%, 100 to 84%, and 100% to 92%, respectively, before and after therapy. Further,hsp60specific amplicons from 23 out of 25 patients gave identical restriction pattern, while 6fliIand 1ureCspecific amplicon produced different restriction pattern. Furthermore, variations infliIgene sequences inH. pyloriafter treatment were also confirmed by sequencing and comparedin silico. Nested PCR based detection ofH. pyloriis more sensitive method to detectH. pyloriafter therapy than culture, RUT, and histology. Further, this study suggests thatH. pyloriis not eradicated completely after triple therapy.

scholarly journals Genotyping and Association of the Presence of Helicobacter Pylori in Dental Plaque and Gastric Biopsy Specimens in Dyspeptic Patients by PCR-RFLP Technique in Southwest of Iran

2021 ◽  
Author(s):  
Mojtaba Moosavian ◽  
Elyas Kushki ◽  
Tahereh Navidifar ◽  
Eskandar Hajiani ◽  
Mahdi Mandegari
Keyword(s):  
Oral Cavity ◽  
Dental Plaque ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Pcr Rflp ◽  
Dental Plaques ◽  
H Pylori ◽  
Southwest Of Iran

Abstract The oral cavity can act as an extra gastric reservoir for H pylori, and also the presence of the bacteria in the oral cavity is associated with a higher risk of dental caries development. The aim of this study was to determine the genotype and evaluate the association of the presence of H. pylori in dental plaque and gastric biopsy specimens in dyspeptic patients in Ahvaz, Southwest of Iran. In this study, 106 patients with recruited dyspeptic complaints were selected and from each patient, two gastric antral biopsy specimens and two dental plagues were examined. The presence of H. pylori was identified by the Rapid Urease Test (RUT) and the amplification of ureAB and 16S rRNA genes. Also, to verify a hypothetical mouth-to-stomach infection route, the enzymatic digestions of three genes of cagA, vacA, and ureAB in H. pylori strains isolated from dental plaques and stomach samples were compared for each same case. H. pylori was found in the stomach of 52.8% (56 /106) and the dental plaques of 17.9% (19/106) of the studied cases. On the other hand, H. pylori was recognized in the stomach of all 19 cases with oral colonization. Following a combination of restriction fragment length polymorphism (RFLP) patterns of these three known genes on stomach and dental plague samples, 14 and 11 unique patterns were seen, respectively. However, for all H. pylori-positive cases (19), the comparison of RLFP patterns of these genes in the dental plaque and gastric biopsy specimens was different for the same case. This study showed, no significant association was observed between the presence of H. pylori in dental plaque and the stomach of the same case.

scholarly journals Epstein-Barr virus detection using gastric biopsy specimens after rapid urease test for Helicobacter pylori

2019 ◽  
Vol 07 (04) ◽  
pp. E431-E432 ◽  
Author(s):  
Hideo Yanai ◽  
Hisashi Iizasa ◽  
Daisuke Chihara ◽  
Tomoyuki Murakami ◽  
Jun Nishikawa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epstein Barr Virus ◽  
Virus Detection ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Barr Virus ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Epstein Barr

Evaluation of a new rapid urease test for Helicobacter pylori in gastric biopsy specimens in pediatric patients

1995 ◽  
Vol 41 (4) ◽  
pp. 336
Author(s):  
JF Fitzgerald ◽  
SKF Chong ◽  
JM Croffie ◽  
JD Rogge ◽  
DR Wagner ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Pediatric Patients ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test

scholarly journals Evaluation of invasive and non-invasive methods for the diagnosis of Helicobacter pylori infection in symptomatic children and adolescents

2001 ◽  
Vol 119 (2) ◽  
pp. 67-71 ◽  
Author(s):  
Silvio Kazuo Ogata ◽  
Elisabete Kawakami ◽  
Francy Reis Silva Patrício ◽  
Margareth Zabeu Pedroso ◽  
Antonio Mario Santos
Keyword(s):  
Helicobacter Pylori ◽  
Children And Adolescents ◽  
Breath Test ◽  
Urea Breath Test ◽  
Diagnostic Methods ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Predictive Values ◽  
Non Invasive ◽  
Urease Test

CONTEXT: Multiple diagnostic methods are available for the detection of Helicobacter pylori infection, but at present no single one can be used as the gold standard. OBJECTIVE: The aim of this study was to evaluate the diagnostic accuracy of 3 invasive and 2 non-invasive methods for detection of Helicobacter pylori infection in symptomatic children and adolescents. DESIGN: Prospective cohort study SETTING: Peptic Disease outpatients service, Discipline of Pediatric Gastroenterology, Universidade Federal de São Paulo / Escola Paulista de Medicina. PATIENTS: Forty-seven patients who underwent endoscopy because of dyspeptic symptoms. DIAGNOSTIC METHODS: Endoscopy with gastric biopsies for 3 invasive (rapid urease test, histology and culture) and 2 non-invasive methods (a commercial ELISA serology and 13carbon urea breath test - isotope ratio mass spectrometry) for detection of Helicobacter pylori infection. MAIN MEASUREMENTS: Sensitivity, specificity, positive and negative predictive values of each method and agreement and disagreement rates between the methods. RESULTS: Forty-seven patients [mean age, 11y9mo (SD 2y10mo), 27 female and 20 male]; 62% of them were Helicobacter pylori-positive. All methods agreed in 61%, and were negative in 21% and positive in 40%. The greatest concordance between 2 methods occurred between the invasive methods: histology and rapid urease test (89.6%) and histology and culture (87.5%). The greatest sensitivity, considering Helicobacter pylori-positive cases, for any combination of 3 or more tests, was achieved by the rapid urease test (S=100%), followed by histology, serology and 13carbon-urea breath test (S=93.1%) and lastly by culture (S=79.3%). The highest specificity was obtained by histology (100%) and culture (100%), followed by the rapid urease test (84.2%), serology (78.9%) and 13carbon-urea breath test (78.9%). CONCLUSIONS: Our results suggest that among invasive methods, an association between the rapid urease test and histology constituted the best choice for the detection of Helicobacter pylori infection. If results of histology and the rapid urease test are different, serology may be recommended.

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