Evaluation of a new rapid urease test for Helicobacter pylori in gastric biopsy specimens in pediatric patients

1995 ◽  
Vol 41 (4) ◽  
pp. 336
Author(s):  
JF Fitzgerald ◽  
SKF Chong ◽  
JM Croffie ◽  
JD Rogge ◽  
DR Wagner ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Pediatric Patients ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test

scholarly journals Epstein-Barr virus detection using gastric biopsy specimens after rapid urease test for Helicobacter pylori

2019 ◽  
Vol 07 (04) ◽  
pp. E431-E432 ◽  
Author(s):  
Hideo Yanai ◽  
Hisashi Iizasa ◽  
Daisuke Chihara ◽  
Tomoyuki Murakami ◽  
Jun Nishikawa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epstein Barr Virus ◽  
Virus Detection ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Barr Virus ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Epstein Barr

Evaluation of rapid urease test for detection of Helicobacter pylori in gastric biopsy specimens

1993 ◽  
Vol 9 (4) ◽  
pp. 450-452 ◽  
Author(s):  
S. Kamiya ◽  
I. Taniguchi ◽  
T. Yamamoto ◽  
T. Shirai ◽  
S. Harasawa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test

An evaluation of the usefulness of invasive and non-invasive methods used to diagnose Helicobacter spp. infections in dogs

2017 ◽  
Vol 20 (3) ◽  
pp. 491-499 ◽  
Author(s):  
M. Jankowski ◽  
J. Spużak ◽  
K. Kubiak ◽  
K. Glińska-Suchocka ◽  
M. Biernat
Keyword(s):  
Nested Pcr ◽  
Gastric Biopsy ◽  
Diagnostic Methods ◽  
Rapid Urease Test ◽  
Pcr Assay ◽  
Non Invasive ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Invasive Method ◽  
Pcr Method

AbstractThe aim of this study was to assess the suitability of invasive and non-invasive methods used to diagnose Helicobacter spp. in the stomachs of dogs. The study was carried out on 30 dogs of both sexes and different breeds, between one and 15 years old. A histopathologic examination, a microbiological culture, a rapid urease test, a direct bacteriological preparation and a nested PCR assay were carried out. Gastric Helicobacter spp. was identified in gastric biopsy specimens from 16 (53.3%) dogs using direct bacteriological preparation, in four (13.3%) dogs based on a culture, in 23 (76.6%) dogs using the rapid urease test and in 21 (70,0%) dogs based on a histopathological assessment of the biopsy specimens. The nested PCR of the gastric biopsy specimens revealed gastric Helicobacter spp. in all the dogs (100%). A saliva PCR assay revealed gastric Helicobacter spp. in 23 (76.6%) dogs, while stool PCR revealed the bacterium in seven (23.3%) dogs. We found that invasive methods were more accurate than non-invasive methods in detecting a Helicobacter spp. infection in dogs. In addition, the nested PCR method used to evaluate the gastric mucosal biopsy specimens was the most accurate test for detecting Helicobacter spp. It was further found that the PCR-based saliva assay was the best non-invasive method for detecting Helicobacter spp. However, taking into consideration that most of the diagnostic methods used to detect this bacterium have drawbacks, at least two diagnostic methods should be used to detect Helicobacter spp. as is done in human medicine.

scholarly journals Genotyping and Association of the Presence of Helicobacter Pylori in Dental Plaque and Gastric Biopsy Specimens in Dyspeptic Patients by PCR-RFLP Technique in Southwest of Iran

2021 ◽  
Author(s):  
Mojtaba Moosavian ◽  
Elyas Kushki ◽  
Tahereh Navidifar ◽  
Eskandar Hajiani ◽  
Mahdi Mandegari
Keyword(s):  
Oral Cavity ◽  
Dental Plaque ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Pcr Rflp ◽  
Dental Plaques ◽  
H Pylori ◽  
Southwest Of Iran

Abstract The oral cavity can act as an extra gastric reservoir for H pylori, and also the presence of the bacteria in the oral cavity is associated with a higher risk of dental caries development. The aim of this study was to determine the genotype and evaluate the association of the presence of H. pylori in dental plaque and gastric biopsy specimens in dyspeptic patients in Ahvaz, Southwest of Iran. In this study, 106 patients with recruited dyspeptic complaints were selected and from each patient, two gastric antral biopsy specimens and two dental plagues were examined. The presence of H. pylori was identified by the Rapid Urease Test (RUT) and the amplification of ureAB and 16S rRNA genes. Also, to verify a hypothetical mouth-to-stomach infection route, the enzymatic digestions of three genes of cagA, vacA, and ureAB in H. pylori strains isolated from dental plaques and stomach samples were compared for each same case. H. pylori was found in the stomach of 52.8% (56 /106) and the dental plaques of 17.9% (19/106) of the studied cases. On the other hand, H. pylori was recognized in the stomach of all 19 cases with oral colonization. Following a combination of restriction fragment length polymorphism (RFLP) patterns of these three known genes on stomach and dental plague samples, 14 and 11 unique patterns were seen, respectively. However, for all H. pylori-positive cases (19), the comparison of RLFP patterns of these genes in the dental plaque and gastric biopsy specimens was different for the same case. This study showed, no significant association was observed between the presence of H. pylori in dental plaque and the stomach of the same case.

Immunological Rapid Urease Test for Detecting Helicobacter pylori: Comparative Study of Biopsy Specimens and Gastric Mucus

1999 ◽  
Vol 11 (1) ◽  
pp. 42-46 ◽  
Author(s):  
Tadashi SATO ◽  
Masayuki A. FUJINO ◽  
Yuichiro KOJIMA ◽  
Fumiaki KITAHARA ◽  
Toshiya NAKAMURA ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Comparative Study ◽  
Rapid Urease Test ◽  
Gastric Mucus ◽  
Biopsy Specimens ◽  
Urease Test

scholarly journals Mutational Analysis of Clarithromycin and Levofloxacin Resistance in Helicobacter pylori from Gastric Biopsy Specimens in a Tertiary Care Hospital in Dhaka, Bangladesh

2019 ◽  
Vol 13 (1) ◽  
pp. 12-19
Author(s):  
Nusrat Noor Tanni ◽  
Shaheda Anwar ◽  
Sharmeen Ahmed ◽  
Kakali Halder ◽  
Maherun Nesa ◽  
...  
Keyword(s):  
Gene Mutations ◽  
Gastric Biopsy ◽  
23S Rrna ◽  
Rapid Urease Test ◽  
Care Hospital ◽  
Gyra Gene ◽  
Biopsy Specimens ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Clarithromycin and Levofloxacin are most frequently included in the standard triple therapies for H. pylori eradication in our country. Resistance to clarithromycin and fluoroquinolones are particularly related with treatment failure. Objectives: The objective of this study was to detect, clarithromycin and levofloxacin resistance associated with gene mutations in H. pylori directly from gastric biopsies using an allele specific primer-PCR (ASP-PCR) assay. Materials and Methods: Gastric biopsy specimens were collected from 143 adult dyspeptic patients, from Department of Gastroenterology, BSMMU and Dhaka Medical College Hospital (DMCH), during the period of March, 2018 to February, 2019. H. pylori was identified by rapid urease test, ureC gene by PCR, histological staining and culture. ASP-PCR was used to identify 23S rRNA gene and gyrA gene mutation predictive of clarithromycin and levofloxacin resistant H. pylori respectively. Results: H. pylori positive cases were 32.9% based on the case definition used in the study. Among 42 ureC positive H. pylori cases, point mutations in 23Sr RNA gene for clarithromycin resistance were detected only at A2142G position in 9 (21.4%) cases and gyrA gene mutations for levofloxacin resistance were detected in 16 (38.1%) cases. Only 1 (2.4%) case had mutation both in 23Sr RNA and gyrA gene. Conclusion: Those findings may guide toward the therapeutic choices in our country. PCR based diagnostic assays can be the alternative approach for rapid detection of antibiotic resistances of H. pylori directly from gastric biopsies, where culture and susceptibility tests are not routinely performed. Bangladesh J Med Microbiol 2019; 13 (1): 12-19

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