scholarly journals Evaluation of Changes in Immune System of Operating Room Personnel by Measurement of Urinary Neopterin Concentrations

Pteridines ◽  
2011 ◽  
Vol 22 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Mustafa Baydar ◽  
Zeynep Capan ◽  
Gozde Girgin ◽  
Terken Baydar ◽  
Gonul Sahin
Keyword(s):  
Immune System ◽  
Operating Room ◽  
High Performance ◽  
Smoking Status ◽  
Diagnostic Value ◽  
Operating Room Personnel ◽  
Macrophage Activity ◽  
Diagnosis And Prognosis ◽  
Urinary Neopterin ◽  
Anesthetic Exposure

Abstract Today it is known that neopterin is mainly produced by activated macrophages and a marker of immune activation and macrophage activity. Increased neopterin concentrations are observed in diseases related to cellular immunity including occupational pathologies.The major goal of the present study was to evaluate the possible alteration of neopterin levels in operating room personnel, and also to show whether screening of neopterin may be useful to monitor the effect of occupational anesthetic exposure on the cellular immune system.Therefore, urinary neopterin to creatinine levels in both, exposed workers (n = 40) and healthy volunteers (n = 30), were measured by using high-performance liquid chromatography. At the same time, the correlation among urinary neopterin levels and working years, age, and smoking status were evaluated.Compared to controls, urinary neopterin levels in the exposed group were increased (controls: 85 ± 16 μmol/mol creatinine, workers: 151 ± 39 μmol/mol creatinine; p <0.05).The findings suggest that the follow up of neopterin levels may have diagnostic value in possible occupational exposure-related immune system disorders. Moreover, its biological monitoring should be performed in workplaces for clinical diagnosis and prognosis.

scholarly journals Effects of Inhalation Anesthetics on the Profile of Urinary Neopterin as an Immune Marker in Patients who Underwent Laparoscopic Cholecystectomy

Pteridines ◽  
2011 ◽  
Vol 22 (1) ◽  
pp. 18-23
Author(s):  
Zeynep Capan ◽  
Terken Baydar ◽  
Gozde Girgin ◽  
Anil Dolgun ◽  
Beyazit Dikmen ◽  
...  
Keyword(s):  
Immune Response ◽  
Laparoscopic Cholecystectomy ◽  
Immune System ◽  
Repeated Measures ◽  
High Performance ◽  
Study Groups ◽  
Urine Samples ◽  
Double Blind ◽  
Inhalation Anesthetics ◽  
Urinary Neopterin

Abstract It has been claimed that inhalational anesthetics affect many phases of immune response. Although, there are a lot of studies on the effects of widely used anaesthetic agents and methods on the immune system, there are only a few studies to show the effects by evaluation of neopterin concentrations as an important marker of cellular immunity. This study was done to evaluate possible effects of inhalation anesthetics, such as desflurane, isoflurane, and sevoflurane on the immune system by measuring urinary neopterin concentrations in patients who underwent laparoscopic cholecystectomy. The study is randomized, prospective and double blind. Urinary neopterin was measured in patients submitted to general anesthesia with either desflurane (n = 20) or isoflurane (n = 11), or sevoflurane (n = 13) in laparoscopic cholecystectomy. Urine samples were collected preoperatively, at 2nd and 24th hours post-operatively and the neopterin per creatinine concentrations were measured by using high-performance liquid chromatography.The preoperative neopterin levels were similar across the study groups. Variance analysis of repeated measures showed an effect of time (p <0.05): Neopterin concentrations increased over time, but the effect of different anesthetics on neopterin was similar. The mean increases in neopterin concentrations were similar among all the applied anesthetics. In conclusion, this report shows changes of immune response by investigation of neopterin in urine samples during 24 hours in patients exposed to three different inhalational anesthetic agents sevoflurane, desflurane and isoflurane in laparoscopic cholecystectomy.

Analysis of the Clinical Diagnostic Value of GMFB in Cerebral Infarction

2020 ◽  
Vol 21 (10) ◽  
pp. 955-963
Author(s):  
Zhaohu Yuan ◽  
Zhiwu Yu ◽  
Yiyu Zhang ◽  
Huikuan Yang
Keyword(s):  
Myocardial Infarct ◽  
Diagnostic Value ◽  
Artery Occlusion ◽  
Response Dynamics ◽  
Stroke Patients ◽  
Ischaemic Injury ◽  
Neuronal Nuclei ◽  
Diagnosis And Prognosis ◽  
Maturation Factor ◽  
Injury And Repair

Background: Glial Maturation Factor Beta (GMFB) is a highly conserved brain-enriched protein implicated in immunoregulation, neuroplasticity and apoptosis, processes central to neural injury and repair following cerebral ischaemia. Therefore, we examined if changes in neurocellular GMFB expression and release can be used to assess brain injury following ischaemia. Methods and Results: Immunofluorescence staining, Western blotting, immunohistochemistry and ELISA were used to measure GMFB in cultured neurons and astrocytes, rat brain tissues and plasma samples from stroke model rats and stroke patients, while cell viability assays, TTC staining and micro- PET were used to assess neural cell death and infarct severity. Immunofluorescence and immunohistochemistry revealed GMFB expression mainly in astrocyte and neuronal nuclei but also in neuronal axons and dendrites. Free GMFB concentration increased progressively in the culture medium during hypoxia-hypoglycaemia treatment. Plasma GMFB concentration increased in rats subjected to middle cerebral artery occlusion (MCAO, a model of stroke-reperfusion) and in stroke patients. Plasma GMFB in MCAO model rats was strongly correlated with infarct size (R2=0.9582). Plasma GMFB concentration was also markedly elevated in stroke patients within 24 h of onset and remained elevated for more than one week. Conversely, plasma GMFB elevations were not significant in myocardial infarct patients and stroke patients without infarction. Conclusion: GMFB has the prerequisite stability, expression specificity and response dynamics to serve as a reliable indicator of ischaemic injury in animal models and stroke patients. Plasma GMFB may be a convenient non-invasive adjunct to neuroimaging for stroke diagnosis and prognosis.

scholarly journals Sphingolipid Analysis Indicate Lactosylceramide as a Potential Biomarker of Inflammatory Bowel Disease in Children

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1083
Author(s):  
Aleksandra Filimoniuk ◽  
Agnieszka Blachnio-Zabielska ◽  
Monika Imierska ◽  
Dariusz Marek Lebensztejn ◽  
Urszula Daniluk
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
High Performance ◽  
Area Under The Curve ◽  
Study Groups ◽  
Diagnostic Value ◽  
Serum Samples ◽  
Specificity And Sensitivity ◽  
Potential Biomarker ◽  
Inflammatory Bowel

An altered ceramide composition in patients with inflammatory bowel disease (IBD) has been reported recently. The aim of this study was to evaluate the concentrations of sphingolipids in the serum of treatment-naive children with newly diagnosed IBD and to determine the diagnostic value of the tested lipids in pediatric IBD. The concentrations of sphingolipids in serum samples were evaluated using a quantitative method, an ultra-high-performance liquid chromatography-tandem mass spectrometry in children with Crohn’s disease (CD) (n=34), ulcerative colitis (UC) (n = 39), and controls (Ctr) (n = 24). Among the study groups, the most significant differences in concentrations were noted for C16:0-LacCer, especially in children with CD compared to Ctr or even to UC. Additionally, the relevant increase in C20:0-Cer and C18:1-Cer concentrations were detected in both IBD groups compared to Ctr. The enhanced C24:0-Cer level was observed only in UC, while C18:0-Cer only in the CD group. The highest area under the curve (AUC), specificity, and sensitivity were determined for C16:0-LacCer in CD diagnosis. Our results suggest that the serum LacC16-Cer may be a potential biomarker that distinguishes children with IBD from healthy controls and differentiates IBD subtypes. In addition, C20:0-Cer and C18:0-Cer levels also seem to be closely connected with IBD.

Intelligent diagnosis and analysis of brain lymphoma based on imaging features

2021 ◽  
pp. 1-11
Author(s):  
Yipu Mao ◽  
Muliang Jiang ◽  
Fanyu Zhao ◽  
Liling Long
Keyword(s):  
Imaging Techniques ◽  
Experimental Studies ◽  
Diagnostic Value ◽  
Imaging Features ◽  
Flow Properties ◽  
Brain Lymphoma ◽  
Diagnosis And Prognosis ◽  
Primary Brain Lymphoma ◽  
Set Up ◽  
Prognosis Evaluation

Currently, DSC has been extensively studied in the diagnosis, differential diagnosis and prognosis evaluation of brain lymphoma, but it has not obtained a uniform standard. By combining DSC imaging features, this study investigated the imaging features and diagnostic value of several types of tumors such as primary brain lymphoma. At the same time, this study obtained data from brain lymphoma patients by data collection and set up different groups to conduct experimental studies to explore the correlation between IVIM-MRI perfusion parameters and DSC perfusion parameters in brain lymphoma. Through experimental research, it can be seen that the combination of two perfusion imaging techniques can more fully reflect the blood flow properties of the lesion, which is beneficial to determine the nature of the lesion.

Health Experiences of Operating Room Personnel

1985 ◽  
Vol 62 (3) ◽  
pp. 325-330 ◽  
Author(s):  
Julie E. Buring ◽  
Charles H. Hennekens ◽  
Sherry L. Mayrent ◽  
Bernard Rosner ◽  
E. Robert Greenberg ◽  
...  
Keyword(s):  
Operating Room ◽  
Operating Room Personnel ◽  
Health Experiences

Oxidative status and DNA damage in operating room personnel

2009 ◽  
Vol 42 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Zeynep Baysal ◽  
Mustafa Cengiz ◽  
Abdullah Ozgonul ◽  
Muslum Cakir ◽  
Hakim Celik ◽  
...  
Keyword(s):  
Dna Damage ◽  
Operating Room ◽  
Oxidative Status ◽  
Operating Room Personnel

scholarly journals The expression of miR-211-5p in atherosclerosis and its influence on diagnosis and prognosis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanxia Zhang ◽  
Huiyun Wang ◽  
Yu Xia
Keyword(s):  
Roc Curve ◽  
Cox Regression ◽  
Survival Curve ◽  
Prognostic Significance ◽  
Significant Negative Correlation ◽  
Diagnostic Value ◽  
Expression Level ◽  
Healthy Controls ◽  
Cox Regression Analysis ◽  
Diagnosis And Prognosis

Abstract Background The purpose of this study was to evaluate the diagnostic and prognostic significance of miR-211-5p in atherosclerosis (AS) by detecting the expression level in serum of patients with AS. Methods A total of 85 healthy controls and 90 asymptomatic AS patients participated in this study. The expression level of miR-211-5p in all subjects were measured by qRT-PCR. Spearman correlation coefficient was used to evaluate the correlation of miR-211-5p with CRP and CIMT. The ROC curve was established to assess the diagnostic value of miR-211-5p in AS. The Kaplan–Meier survival curve and multivariate COX regression analysis were used to evaluate the prognostic significance of miR-211-5p in AS. Results The expression levels of miR-211-5p in AS patients were significantly lower than in healthy controls (P < 0.001), and miR-211-5p showed a significant negative correlation with CRP (r =  − 0.639, P < 0.001) and CIMT (r =  − 0.730, P < 0.001). The AUC of the ROC curve was 0.900, the specificity and the sensitivity were 84.7% and 78.9%, respectively, which indicating that miR-211-5p had diagnostic value for AS. Survival analysis showed that patients with low miR-211-5p expression were more likely to have cardiovascular end-point events (Log rank P = 0.013). Conclusion Serum miR-211-5p could be used as a new biomarker for the diagnosis of AS, and the low expression of miR-211-5p is associated with the poor prognosis of AS.

Lack of Mutagens in Urines of Operating Room Personnel

1980 ◽  
Vol 53 (3) ◽  
pp. 195-198 ◽  
Author(s):  
Jeffrey M. Baden ◽  
Merijean Kelley ◽  
Andrew Cheung ◽  
Kristien Mortelmans
Keyword(s):  
Operating Room ◽  
Operating Room Personnel

Assessment of prenatal tobacco smoke exposure by determining nicotine and its metabolites in meconium

2007 ◽  
Vol 26 (6) ◽  
pp. 535-544 ◽  
Author(s):  
E. Köhler ◽  
S. Avenarius ◽  
A. Rabsilber ◽  
C. Gerloff ◽  
G. Jorch
Keyword(s):  
Tobacco Smoke ◽  
Maternal Smoking ◽  
High Performance ◽  
Smoking Status ◽  
Hplc Method ◽  
Smoke Exposure ◽  
Tobacco Smoke Exposure ◽  
Smoking During Pregnancy ◽  
Quit Smoking

Meconium samples collected from 115 neonates were analysed for nicotine, cotinine and trans -3-hydroxycotinine (OH-cotinine) by means of high-performance liquid chromatography (HPLC) to identify prenatal smoke exposure. The self-reported maternal smoking status during pregnancy was determined by means of a questionnaire and verified by measurements in urine prior to childbirth. The total sum of nicotine and its metabolites (Sumtot) of the first passed meconium samples was 1560 ± 1024 pmol/g in newborns of smoking mothers. Smoking of less than five cigarettes was clearly detected. Sumtot remained constant in all meconium samples passed by a neonate in succession. However, the proportion of nicotine decreased with the time of passage after birth and the OH-cotinine proportion increased, whereas cotinine hardly changed. Nicotine or its metabolites were not detectable in meconium (detection limit < 20 pmol/g), when the mothers were only exposed to environmental tobacco smoke (ETS) using the HPLC method. The hypothesis that the content of nicotine metabolites in meconium reflects long-term smoke exposure could not be confirmed in newborns whose mothers had quit smoking during the latter half of pregnancy. Determining Sumtot enables the intensity of continuous smoking during pregnancy to be estimated in all meconium samples passed by a newborn. Human & Experimental Toxicology (2007) 26: 535—544

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