scholarly journals Novel nuclear microsatellites in the endangered neotropical timber tree Lecythis ampla (Lecythidaceae)

2014 ◽  
Vol 63 (1-6) ◽  
pp. 181-184
Author(s):  
G. Hernández ◽  
H. Konrad ◽  
S. Krumböck ◽  
Thomas Geburek
Keyword(s):  
Null Alleles ◽  
Molecular Tools ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Dna Libraries ◽  
Timber Tree ◽  
Nuclear Microsatellite ◽  
Enrichment Protocol ◽  
Candidate Loci ◽  
First Time

AbstractFor the first time, nuclear microsatellite (nSSRs) primers were developed in the endangered tree species Lecythis ampla (Lecythidaceae) as molecular tools. An enrichment protocol with genomic DNA libraries for nSSRs was used to identify candidate loci. A large number of candidate loci were identified. Consecutively population genetic parameters of these loci were tested in two available populations. Eventually 17 microsatellite loci have been identified that show no or only low evidence for linkage disequilibrium, deviations from Hardy-Weinberg expectations or high levels of null alleles. These markers are apt for future molecular population studies.

scholarly journals Mating system and population structure in the natural distribution of Toona ciliata (Meliaceae) in South China

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wei Zhou ◽  
Xin-Xin Zhang ◽  
Ying Ren ◽  
Pei Li ◽  
Xiao-Yang Chen ◽  
...  
Keyword(s):  
Mating System ◽  
South China ◽  
Isolation By Distance ◽  
Outcrossing Rate ◽  
Population Substructure ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Multiple Loci ◽  
Toona Ciliata ◽  
Nuclear Microsatellite

Abstract Most initially perfect flowers of Toona ciliata Roem subsequently develop into functionally unisexual flowers and their relative positions in the same inflorescence could enhance the outcrossing system in this species. Here we investigated the mating system of this species. We used eight nuclear microsatellite markers and investigated the progeny of 125 mother trees from six populations naturally distributed in South China, with sample sizes ranging from 64 to 300 seeds. The multilocus outcrossing rate was 0.970 ± 0.063, and the single locus outcrossing rate was 0.859 ± 0.106, indicating the pattern of predominant outcrossing. Selfing was present in one population, but biparental inbreeding occurred in five populations. Inbreeding was absent in maternal parents, and correlations of selfing among families or among loci were generally insignificant. Positive correlation of paternity at multiple loci was significant in four populations, but was not consistent with the results at single loci. Population substructure occurred in male similarity between outcrosses only in one population. Population genetic differentaitaion was significant (Fst = 34.5%) and the effects of isolation-by-distance at the eight loci were significant among the six populations. These results provide evidence that self-comptability and inbreeding naturally occur in T. ciliata and indicate that inbreeding avoidance is necessary during genetic improvement and breeding of this endangered tree species.

scholarly journals Discovery of novel SSR markers from transcriptome data of Astronium fraxinifolium Schott, a threatened tree species in Brazil

2020 ◽  
Author(s):  
Maiara Cornacini ◽  
Ricardo Oliveira Manoel ◽  
Marcelo Alcantara ◽  
Mário Moraes ◽  
Edvaldo Silva ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Tree Species ◽  
Natural Populations ◽  
Transcriptome Data ◽  
Rna Seq ◽  
Endangered Tree ◽  
Genetic Studies ◽  
Endangered Tree Species ◽  
Ssr Loci ◽  
First Time

Abstract Background Astronium fraxinifolium is an endangered tree species from Brazil. Due to its high importance for environmental reforestation, as well as for the use of its wood, it is necessary to implement management programs for conservation of this species. Simple sequence repeats (SSR) or microsatellite markers have been widely used in population genetic studies across diverse organisms. In this study, we reported for the first time SSR markers for A. fraxinifolium as well as its frequency and distribution from transcriptome data. Results More than 125 thousand RNA-seq sequences derived microsatellites, with predominant distribution of trinucleotides repeats. From the initial screening, we selected 20 microsatellite loci, validated and evaluated genetic indexes in two natural populations. All loci were polymorphic, ranging from four to eleven alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively. Genetic differentiation between populations (= 0.363) showed higher diversity within than among populations. Conclusions The developed SSR loci from RNA-seq consists in a base for future studies of genetic diversity and population structure, mating system and gene flow in A. fraxinifolium populations as well as related species, aiming the conservation and management of the species.

Floristic Variation of Tree Communities and Their Association with Soil Properties in Pulau Jerejak, Penang, Peninsular Malaysia

2020 ◽  
Vol 14 (1) ◽  
pp. 34
Author(s):  
Faezah Pardi
Keyword(s):  
Tree Species ◽  
Diversity Index ◽  
Basal Area ◽  
Peninsular Malaysia ◽  
Importance Value ◽  
Conservation Action ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Tree Communities ◽  
Floristic Variation

This study was conducted at Pulau Jerejak, Penang to determine the floristic variation of its tree communities. A 0.5-hectare study plot was established and divided into 11 subplots. A total of 587 trees with diameter at breast height (DBH) of 5 cm and above were measured, identified and recorded. The tree communities comprised of 84 species, 63 genera and 32 families. The Myrtaceae was the most speciose family with 10 recorded species while Syzgium glaucum (Myrtaceae) was the most frequent species. The Myrtaceae recorded the highest density of 306 individuals while Syzgium glaucum (Myrtaceae) had the highest species density of 182 individuals. Total tree basal area (BA) was 21.47 m2/ha and family with the highest BA was Myrtaceae with 5.81 m2/ha while at species level, Syzgium glaucum (Myrtaceae) was the species with the highest total BA in the plot with value of 4.95 m2/ha. The Shannon˗Weiner Diversity Index of tree communities showed a value of 3.60 (H'max = 4.43) and Evenness Index of 0.81 which indicates high uniformity of tree species. The Margalef Richness Index (R') revealed that the tree species richness was 13.02. Myrtaceae had the highest Importance Value of 20.4%. The Canonical Correspondence Analysis (CCA) showed that Diospyros buxifolia (Ebenaceae) and Pouteria malaccensis (Sapotaceae) were strongly correlated to low pH. Dysoxylum cauliflorum (Meliaceae) and Eriobotrya bengalensis (Rosaceae) were correlated to phosphorus (P) and calcium ion (Ca2+), respectively. Therefore, the trees species composition at Pulau Jerejak showed that the biodiversity is high and conservation action should be implemented to protect endangered tree species. Keywords: Floristic variation; Tree communities; Trees composition; Pulau Jerejak; Species diversity

Somatic embryogenesis and plantlet formation from a rare and endangered tree species,Oplopanax elatus

2006 ◽  
Vol 49 (4) ◽  
pp. 320-325 ◽  
Author(s):  
Heung Kyu Moon ◽  
Ji Ah Kim ◽  
So Young Park ◽  
Yong Wook Kim ◽  
Ho Duck Kang
Keyword(s):  
Somatic Embryogenesis ◽  
Tree Species ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Plantlet Formation ◽  
Rare And Endangered ◽  
Oplopanax Elatus

scholarly journals Potential natural enemies of the invasive rugose spiraling whitefly, Aleurodicus rugioperculatus Martin in India

2017 ◽  
Vol 30 (4) ◽  
pp. 236 ◽  
Author(s):  
K. Selvaraj ◽  
R. Sundararaj ◽  
T. Venkatesan ◽  
Chandish R. Ballal ◽  
S. K. Jalali ◽  
...  
Keyword(s):  
Natural Enemies ◽  
Tamil Nadu ◽  
Ornamental Plants ◽  
Coi Gene ◽  
Molecular Diagnostic ◽  
Pest Species ◽  
Molecular Tools ◽  
Accession Number ◽  
Custard Apple ◽  
First Time

A invasive rugose spiraling whitefly (RSW) <em>Aleurodicus rugioperculatus</em> Martin (Hemiptera: Aleyrodidae) was found infesting coconut, banana, custard apple and several ornamental plants in Tamil Nadu, Andhra Pradesh and Kerala for the first time in India. The identity of the pest species was determined through morphological and molecular tools. Furthermore cytochrome c oxidase-I gene (658 bp) of RSW was sequenced (GenBank accession number KY209909) which would serve as an ideal molecular diagnostic marker for its identification irrespective of its phenotypic plasticity. During the survey, several natural enemies were recorded and maximum parasitism was recorded by <em>Encarsia guadeloupae</em> Viggiani (Hymenoptera: Aphelinidae) and its COI gene was sequenced and deposited as <em>Encarsia</em> sp. (GenBank accession number KY223606). Per cent parasitism ranged from 20.0 to 60.0 % in different collection locations, highest parasitism being recorded in Kerala as compared to other states. The predators recorded were <em>Mallada</em> sp., few coccinellids and predatory mites. This communication is the first report of the rugose spiraling whitefly, its host plant range and associated natural enemies in India.

Indopiptadenia Oudhensis (Brandis) Brenan an Endangered Tree Species: Measures for the Coservation

2014 ◽  
Vol 37 (1) ◽  
pp. 69-72
Author(s):  
Giriraj Panwar ◽  
Kumar Ambrish ◽  
S. Srivastava
Keyword(s):  
Seed Germination ◽  
Tree Species ◽  
Seed Viability ◽  
Habitat Destruction ◽  
Anthropogenic Pressure ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Poor Seed ◽  
Over Exploitation

Indopiptadenia oudhensis (Brandis) Brenan is an endangered tree species of family Mimosaceae. Species is mainly distributed at Indo-Nepal border and facing threats such as anthropogenic pressure, habitat destruction, over exploitation, low seed viability and poor seed germination.

Impact of soil salinity on growth performance of the plant Syzygium travancoricum Gamble. in their natural habitat - A critically endangered tree species of Southern Western Ghats

2015 ◽  
Vol 22 (4) ◽  
pp. 219-223
Author(s):  
Abdul Hussain ◽  
C. Anilkumar
Keyword(s):  
Soil Salinity ◽  
Natural Habitat ◽  
Critically Endangered ◽  
High Rate ◽  
Optimum Level ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Critically Endangered Species ◽  
Nutrients Uptake ◽  
Southern Western Ghats

Increased rate of soil salinity caused changes in macro and micro nutrients uptake, which may lead to decline in photosynthesis capacity and respiration in the plant Syzgium travancoricum Gamble. is a critically endangered species located in damp forest with marshy land, where high rate of salt accumulation has been happening. An optimum level of salinity (0.05 - 0.07) is inevitable for the proper growth and survivability of this species in their natural habitat and also for natural regeneration. A range of reduction in the number of existing plants of this species can be seen in their natural habitat due to the salinity stress.

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