Changes in oxidative damage and antioxidant enzyme activities of barley (Hordeum vulgare L.) cultivars exposed to rewarming upon freezing stress / Donma stresi üzerine yeniden sıcaklık artışına maruz kalmış arpa (Hordeum vulgare L.) çeşitlerinin antioksidan enzim aktivitelerinde ve oksidatif zararında değişimler

AbstractObjective: The growth of plants during recovery period after freezing stress is more important for winter cereals to survive frost. However, little research has been done on physio-biochemical changes in response to recovery or rewarming conditions in cereals. In this study, the changes in oxidative stress intensities and antioxidant enzyme activities were thus examined under cold acclimation (CA), short-term freezing stress (F) and rewarming (R) conditions in barley cultivars differing in cold tolerance in order to assess the changes in Reactive Oxygen Species (ROS) scavenging system.Methods: Electrolyte leakage, Fv/Fm ratio (quantum efficiency of PSII), the contents of hydrogen peroxide (HResults: A corresponding increase in the free proline content and activities of APX and GR, the levels of Fv/Fm ratio, electrolyte leakage and the contents of MDA and HConclusion: Lower amount of H

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Superoxide dismutase, catalase, and glutathione peroxidase during epididymal maturation and prolonged storage of spermatozoa in the Mexican big-eared bat (Corynorhinus mexicanus)

Canadian Journal of Zoology ◽

10.1139/z05-152 ◽

2005 ◽

Vol 83 (12) ◽

pp. 1556-1565 ◽

Cited By ~ 5

Author(s):

E Arenas-Ríos ◽

M A León-Galván ◽

P E Mercado ◽

A Rosado

Keyword(s):

Superoxide Dismutase ◽

Antioxidant Enzyme ◽

Reproductive Cycle ◽

Enzyme Activities ◽

Storage Period ◽

Prolonged Storage ◽

Ros Generation ◽

Antioxidant Enzyme Activities ◽

Ros Scavenging ◽

Epididymal Spermatozoon

We studied the activities of reactive oxygen species (ROS) scavenging enzymes during epididymal spermatozoon maturation and storage in Corynorhinus mexicanus (G.M. Allen, 1916), a vespertilionid bat that stores spermatozoa in the epididymides for several months after regression of the testes. Depending on the phase of the epididymal reproductive cycle, two different patterns of antioxidant enzyme activities were observed in C. mexicanus. Catalase activity is clearly present in both caput and cauda epididymides throughout the entire annual reproductive cycle, being particularly high during the post-testicular phase of epididymal function. Superoxide dismutase (SOD) activity, present during the testicular phase of epididymal transport and maturation of spermatozoa, is almost completely absent or inhibited in both epididymal segments during the post-testicular epididymal storage period. GPx activity is low during the testicular phase of epididymal spermatozoon maturation and is high in both epididymal segments during the storage phase of epididymal function. From our results, we postulate that (i) the pattern of epididymal antioxidant enzyme activities in C. mexicanus is significantly different from the pattern that is proposed to be unique for mammals; (ii) epididymal function in these species of bats can be clearly divided into two phases, a testicular-dependent phase that is related to the spermatozoon maturation function of the epididymides and a testicular-independent phase that is related to the long-term spermatozoon storage function observed in these mammals; (iii) the study of the regulation of the redox potential of the microenvironment, associated with mammalian spermatozoa as they transit through the epididymides, must be particularly focused on the anatomical region where ROS generation scavenging and spermatozoon maturation storage processes take place.

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Physiological and biochemical changes in waterlog tolerant sesame genotypes

SAARC Journal of Agriculture ◽

10.3329/sja.v14i2.31243 ◽

2017 ◽

Vol 14 (2) ◽

pp. 31-45 ◽

Cited By ~ 3

Author(s):

RR Saha ◽

F Ahmed ◽

N Mokarroma ◽

MM Rohman ◽

PC Golder

Keyword(s):

Antioxidant Enzyme ◽

Enzyme Activities ◽

Antioxidant Activities ◽

Recovery Period ◽

Control Plant ◽

Vegetative Stage ◽

Antioxidant Enzyme Activities ◽

Waterlogging Tolerance ◽

Mda Content ◽

Sesame Genotypes

An experiment was conducted under pot culture to investigate physiological responses as well as antioxidative enzymes activities that may lead to select sesame genotype (s) which were more waterlogging tolerant at vegetative stage. Four sesame genotypes viz. BD-6980, BD- 6985, BD-6992 and BD-7012 were grown under waterlogged (at vegetative stage) and control (no waterlogged) conditions. Plant height, root length, root volume, root dry weight and leaf area per plant in all the four sesame genotypes significantly decreased due to waterlogging at vegetative stage in comparison to controlled condition. Higher SPAD value (Soil and Plant Analyzer Development) and specific leaf mass were recorded in waterlogged plant than controlled plant during waterlogging period but reverse was the case during recovery period. All the genotypes showed positive indices of waterlogging tolerance in terms of better performance of root, stem, leaf and petiole. Among the genotypes, BD 6980 showed higher waterlogging tolerance in all the components followed by BD 6985. Malondialdehyde (MDA) content was found higher in waterlogged plant of all the four sesame genotypes in both waterlogging period and recovery period than the controlled plant. Antioxidant enzyme activities like Peroxidase (POD), Catalase (CAT), Ascorbate peroxidase (APX), Glutathione peroxidase (GPX) and Superoxide dismutase(SOD) were inconsistent in the present study but most of the antioxidant enzyme activities showed an increasing trend in waterlogged plant than that of control plant in all the genotypes. Comparatively low amount of MDA content and high antioxidant activities of sesame genotype BD 6980 is considered as highly tolerant to waterlogging and other three genotypes are moderately tolerant under water logging condition.SAARC J. Agri., 14(2): 31-45 (2016)

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Enhanced antioxidant enzyme activities in developing anther contributes to heat stress alleviation and sustains grain yield in wheat

Functional Plant Biology ◽

10.1071/fp19016 ◽

2019 ◽

Vol 46 (12) ◽

pp. 1090 ◽

Cited By ~ 3

Author(s):

Sharad K. Dwivedi ◽

Sahana Basu ◽

Santosh Kumar ◽

Surbhi Kumari ◽

Alok Kumar ◽

...

Keyword(s):

Heat Stress ◽

Grain Yield ◽

Antioxidant Enzyme ◽

Heat Tolerance ◽

Enzyme Activities ◽

Redox Homeostasis ◽

Antioxidant Enzyme Activities ◽

Ros Scavenging ◽

Wheat Genotype ◽

Wheat Genotypes

Climatic variations along with a rise in temperature during the winter season impose severe heat stress during the anthesis stage of spring wheat, resulting in severe yield losses. The present study was conducted to evaluate the influence of heat stress on redox homeostasis in developing anthers and flag leaves of wheat. Five Indian bread wheat genotypes were studied under field conditions during the dry season, with two extreme sowing dates (timely and very late sown) to explore the effect of heat stress on anthesis stage. Results showed that elevated temperature during anthesis caused significant increase in reactive oxygen species (ROS) content and malondialdehyde (MDA) accumulation in developing anthers, triggering pollen mortality. Moreover, defective source (leaf) to the sink (anthers) mobilisation of starch also contributes in reducing pollen viability. However, ROS-induced oxidative damage of developing anthers under heat stress varied among the wheat genotypes depending upon differential antioxidant enzyme activities. Wheat genotype with enhanced antioxidant activities and reduced ROS built up in developing anthers sustained their grain yield, suggesting thermo-tolerance in wheat to be associated with antioxidant enzyme-mediated improved ROS-scavenging mechanism not only in leaves even in developing anther also. In the present study, heat stressed wheat genotype WH 730 exhibited effective source to sink mobilisation and sustainable grain yield with improved ROS scavenging, conferring greater potential for heat tolerance. We conclude that redox homeostasis and balanced source sink activity played a significant role for sustainable yield and heat tolerance in wheat.

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Effect of Exogenous Treatment with Nitric Oxide (NO) on Redox Homeostasis in Barley Seedlings (Hordeum vulgare L.) Under Copper Stress

Journal of soil science and plant nutrition ◽

10.1007/s42729-021-00757-w ◽

2022 ◽

Author(s):

Marouane Ben Massoud ◽

Oussama Kharbech ◽

Yathreb Mahjoubi ◽

Abdelilah Chaoui ◽

Astrid Wingler

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Lipid Peroxidation ◽

Hordeum Vulgare ◽

Antioxidant Enzyme ◽

Enzyme Activities ◽

Redox Homeostasis ◽

Copper Stress ◽

Antioxidant Enzyme Activities ◽

Cu Toxicity

AbstractThe protective mechanism of nitric oxide (NO) in regulating tolerance to Cu-induced toxicity in shoots of barley (Hordeum vulgare L.) was studied. The experiment consisted of four treatments based on additions to basal nutrient solutions (BNS): control (CTR), Cu (200 µM), SNP (500 µM), and Cu (200 µM) + SNP (500 µM) over a period of 10 days. Treatment with Cu significantly reduced seedling growth and photosynthetic efficiency concomitant with an increase in reactive oxygen species contents, lipid peroxidation markers, and antioxidant enzyme activities, indicating that Cu induced oxidative stress. Furthermore, growth inhibition of Cu-treated plants was associated with a reduction in photosynthetic pigments and maximum photosystem II efficiency as well as a strong decrease in levels of glutathione (GSH) and ascorbate (AsA). Addition of a nitric oxide (NO) donor, sodium nitroprusside (SNP), to the growth medium alleviated Cu toxicity by decreasing Cu uptake and enhancing antioxidant capacity, as indicated by increased contents of GSH and AsA. The application of SNP decreased oxidative stress and lipid peroxidation by suppressing lipoxygenase activity and enhancing some antioxidant enzyme activities. The results obtained indicate the potential of exogenously applied SNP in the management of metal toxicity. Hence, NO generating compounds have potential agronomical applications when cultivating in contaminated areas. Our findings indicate that NO can alleviate Cu toxicity by affecting the antioxidant defense system and maintaining the glutathione-ascorbate cycle status, suggesting that SNP treatment protects proteins against oxidation by regulating the cellular redox homeostasis.

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