scholarly journals Comparison of enzyme-linked fluorescent assay and electrochemiluminescence immune assay in procalcitonin measurement

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Burçin Erdem Kinas ◽  
Arzu Etem Akagac ◽  
Aybala Erek Toprak ◽  
Sule Batcık ◽  
Ahmet Rıza Uras
Keyword(s):  
Intensive Care ◽  
Arithmetic Mean ◽  
Good Precision ◽  
Altman Analysis ◽  
Sepsis Severity ◽  
Fluorescent Assay ◽  
Bland Altman Analysis ◽  
Intensive Care Patients ◽  
The Difference ◽  
Immune Assay

Abstract Background Procalcitonin (PCT) measurement is required for intensive care patients with systemic inflammation symptoms, early diagnosis of possible infections, and evaluation of sepsis severity and prognosis. Objectives We aimed to determine the analytical performance of PCT measurement in a Roche Modular E170 (ECLIA) analyzer and compare the performance with VIDAS (BRAHMS/ELFA) analyzer findings. Material and methods Within-day and between-day precision value, linearity was determined, and two methods were compared with regression and Bland–Altman analysis. Results Both ECLIA and ELFA assays indicated excellent precision, where within-day precision varied between 1.18% and 3.97% CV, and between-day precision varied between 1.77% and 3.93% CV. The ECLIA method was linear up to 62.15 ng/mL. The arithmetic mean was 6.02 ng/mL with the ECLIA method and 8.02 ng/mL with the ELFA method. The correlation coefficient was r=0.996 and p=0.001. The correlation was linear between the two methods. Regression equation was found y=0.78x − 0.23. The Bland–Altman figure was revealed the difference between the methods was specifically in lower concentrations (<0.15 ng/mL). Conclusions Both methods show good precision and correlation. It was determined that the difference between methods was significant, especially at <0.15 ng/mL concentration.

Electronic Nose Prediction of a Clinical Pneumonia Score: Biosensors and Microbes

2005 ◽  
Vol 102 (1) ◽  
pp. 63-68 ◽  
Author(s):  
C William Hanson ◽  
Erica R. Thaler
Keyword(s):  
Electronic Nose ◽  
Breath Test ◽  
Altman Analysis ◽  
Surgical Intensive Care ◽  
Test Analysis ◽  
Mechanically Ventilated ◽  
Bland Altman Analysis ◽  
Intensive Care Patients ◽  
A Prospective Study ◽  
Expiratory Limb

Background The authors performed a prospective study to determine whether breath test analysis using an electronic nose correlates with a clinical pneumonia score. Methods Exhaled gas was sampled from the expiratory limb of the ventilator in mechanically ventilated surgical intensive care patients and assayed with the electronic nose. Components of a clinical pneumonia score were recorded concurrently. Results The score predicted by the electronic nose showed good correlation with the actual pneumonia score (r = 0.81). Bland Altman analysis showed a mean bias of 0.0 (limits +/- 2.6). Conclusions The electronic nose is a new biosensor technology that correlates with a clinical pneumonia score.

scholarly journals Reliability and Validity of an Ultrasonic Device for Measuring Height in Adults

2021 ◽  
Vol 42 (5) ◽  
pp. 376-381
Author(s):  
Seon Hwa Cho ◽  
Young Gyu Cho ◽  
Hyun Ah Park ◽  
A Ra Bong
Keyword(s):  
Correlation Coefficient ◽  
Health Surveys ◽  
Intraclass Correlation ◽  
Reliability And Validity ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Community Health Survey ◽  
Measurement Devices ◽  
The Difference ◽  
Very High

Background: The ultrasonic stadiometer was originally developed as a device to measure and monitor children’s height. However, an ultrasonic stadiometer (InLab S50; InBody Co., Seoul, Korea) was used to measure adults’ height in the 2018 Korea Community Health Survey (KCHS). This study was conducted to assess the reliability and validity of the InLab S50 in adults. Methods: The study subjects were 120 adults (reliability test, n=20; validity test, n=100) who had visited a health screening center. The intra- and inter-rater reliabilities of InLab S50 were assessed using the intraclass correlation coefficient (ICC). The agreement between InLab S50 and an automatic stadiometer (HM-201; Fanics, Busan, Korea) was assessed using Pearson’s correlation coefficient and Bland-Altman analysis. Results: The intra- and inter-rater reliabilities of the InLab S50 were excellent (ICC=0.9999 and 0.9998, respectively). The correlation coefficient of the height measured by the two measurement devices was very high (r=0.996). The difference (Δheight [HM-201-InLab S50]) was -0.15±0.78 cm (95% limit of agreement [LOA], -1.69 to 1.38). After excluding the values outside 95% LOA, the difference was further reduced to -0.05±0.59 cm (95% LOA, -1.20 to 1.10). Conclusion: This study showed that the InLab S50 is a reliable and valid device for the measurement of adults’ height. Therefore, we think that InLab S50 could be used to measure adults’ height in household health surveys such as the KCHS.

Relative validity and repeatability of a Zinc-FFQ for estimating intakes of Iron, Copper and selenium in Saudi adults

2020 ◽  
Vol 16 ◽  
Author(s):  
Alsufiani Hadeil M ◽  
Albar Salwa A ◽  
Al-shehri Aishah D ◽  
Al-Mufti Zain A ◽  
Aldhahri Rahaf S ◽  
...  
Keyword(s):  
Deficiency Anemia ◽  
Altman Analysis ◽  
Food Record ◽  
Food Frequency ◽  
Relative Validity ◽  
Bland Altman Analysis ◽  
Iron Copper ◽  
The Mean ◽  
The Difference ◽  
Highly Correlated

Background: In Saudi Arabia, few studies have addressed iron, selenium and copper intake and status. Most studies of iron have focused on iron deficiency anemia rather than the intake of iron. Objective: To assess the relative validity and repeatability of a Zinc-Food Frequency Questionnaire (FFQ) for estimating the intakes of iron, copper and selenium in Saudi adults. Method: Ninety-seven Saudi adults were participated in this study. All participants completed the FFQ1 and a three-days (3d) Food Record (FR) to assess validity. After one month, the FFQ was administered again (FFQ2) to assess the repeatability. Results: Mean intakes of iron, copper and selenium estimated by FFQ1 were significantly higher than those from the 3d FR. Estimated intakes of iron and selenium by both methods were significantly correlated. The Bland-Altman analysis showed that the difference in iron intakes, as measured by the two methods, was similar across the range of intakes while the difference in copper and selenium intakes increased with an increase in the mean intake. No detectable differences were observed between FFQ1 and FFQ2 in estimating iron, copper and selenium intakes. For all nutrients, the intakes from both FFQs were highly correlated. Conclusion: The Zinc-FFQ showed reasonable relative validity and repeatability for estimating other micronutrients, such as iron, copper and selenium.

Verification of the accuracy and reliability of the TrackMan tennis radar

2020 ◽  
pp. 175433712095300
Author(s):  
Munenori Murata ◽  
Hiroo Takahashi
Keyword(s):  
Measurement Accuracy ◽  
Intraclass Correlation ◽  
Correlation Coefficients ◽  
Spin Axis ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Practical Applications ◽  
Intraclass Correlation Coefficients ◽  
The Difference ◽  
Proven Method

TrackMan, a device used to measure the kinematics of a ball in flight, has recently been used for research and coaching purposes in tennis, and the number of studies on this device is expected to increase. This study is focused on verifying the accuracy of TrackMan; three participants performed services with various velocities and angular velocity combinations. TrackMan and a proven method based on computer vision were used to measure the speed, spin rate, and spin axis of the ball. The results obtained using each method were compared using regression analysis, intraclass correlation coefficients, and Bland–Altman analysis. The speed and spin rate of the two methods agreed well; the degree of error would not be problematic in practical applications. However, TrackMan underestimated the speed if it was unable to detect the hitting position. The spin axis measured by TrackMan was unstable under low spin rate conditions (<3000 rpm). Furthermore, the standard deviation of the spin axis measurement error exceeded the difference between the average slice and kick service spin axes reported in the previous study, so the spin axis measurement accuracy was very low for research purposes, even when the spin rate was above 3000 rpm. For those who are aware of the existence of outliers, TrackMan can be useful for coaching because it provides instant feedback. Similarly, the measured rotational speed and spin rate could be used in the study.

Faster HbA1c Turnaround Times Using a Dedicated Analyzer Based on Turbidimetric Inhibition Immunoassay Technology

2020 ◽  
Author(s):  
Chin Shern Lau ◽  
Melva Lim ◽  
See Ping Hoo ◽  
Cherlyn Gan ◽  
Tar Choon Aw
Keyword(s):  
Complete Blood Count ◽  
Point Of Care ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Deming Regression ◽  
Significant Difference ◽  
The Difference ◽  
Interassay Precision ◽  
Hba1c Levels ◽  
Comparable Time

Abstract Introduction The Roche Cobas c513 (c513) is a dedicated stand-alone high throughput HbA1c analyzer. We evaluated the performance and the difference in turnaround times (TAT) of the c513 against our Cobas 8000 c502 (c502). Methods We assessed the linearity and precision of the c513, and its agreement (Deming regression and Bland–Altman analysis) with the c502 assay. We compared TAT for these analyzers for a single run of 50 samples and for all samples run over 2 comparable time periods. Results The c513 assay was linear from 4.4–18.3% HbA1c. Interassay precision (CV%) was 1.2 and 0.8 at HbA1c levels of 5.7 and 10.5%, respectively. The c513 assay showed excellent concordance with the c502 assay (r = 0.997) with no significant difference between methods by Bland–Altman analysis (mean difference = 0.021% HbA1c, P = 0.1422). The c513 took 17 min to analyze 50 samples, compared to 40 min on the c502. Over comparable 2-month periods, 90% of samples requiring HbA1c tests only were completed under 25 min (c513) vs 30–35 min (c502). For tubes sharing complete blood count (CBC) testing with HbA1c, the 90th percentile TAT was 35–40 min (c513) compared to 45–50 min (c502). Conclusion The c513 assay performs well with excellent correlation to the c502 assay. The improved TAT of the c513 is suitable when there are demands for rapid HbA1c results and it may forestall requests for point-of-care testing. It is also attractive to sites with heavy workloads with a claimed throughput of 400 tests / h.

scholarly journals Short Answer Open-Ended versus Multiple-Choice Questions: A Comparison of Objectivity

2016 ◽  
Vol 52 (03) ◽  
pp. 173-182
Author(s):  
Bharati Mehta ◽  
Bharti Bhandari ◽  
Parul Sharma ◽  
Rimplejeet Kaur
Keyword(s):  
Multiple Choice ◽  
Answer Sheet ◽  
Altman Analysis ◽  
Multiple Choice Questions ◽  
Bland Altman Analysis ◽  
Short Answer ◽  
Final Score ◽  
The Difference ◽  
Written Test

Abstract Objectives: We designed our study with the hypothesis that open ended Short Answer type Questions (SAQs), no matter how carefully framed, cannot be as objective as Multiple Choice type Questions (MCQs). Methods: The study was conducted on 1st year MBBS students (n=99) studying at AIIMS, Jodhpur. A written test on 'Blood & Immunity' was conducted containing same questions in two formats; twelve MCQs (type E) in section A and 12 SAQs in section B. Maximum marks for all questions in both sections were equal. All the answers of section B were evaluated separately by two different examiners to reduce the subjectivity and a model answer sheet for both the sections was prepared and provided to both the examiners. Results: The difference in the scores in Section B SAQs that were evaluated by two different examiners was not statistically significant. Mean of the marks awarded by the two examiners was taken as the final score of each student in section B. The difference in the scores by the students in the two sections was also non-significant (p=0.14). A significant correlation (r=0.99, p<0.0001) was found in SAQ and MCQ scores. Bland- Altman analysis also showed no proportion of bias and the two methods of scoring were in agreement with each other. Conclusion: The results suggest that meticulously-framed open-ended short answer type questions can be as objective as multiple choice type questions.

scholarly journals Absolute Quantification of Cerebral Blood Flow with Magnetic Resonance, Reproducibility of the Method, and Comparison with H215O Positron Emission Tomography

2002 ◽  
Vol 22 (9) ◽  
pp. 1149-1156 ◽  
Author(s):  
Timothy J. Carroll ◽  
Vincenzo Teneggi ◽  
Mathieu Jobin ◽  
Lisa Squassante ◽  
Valerie Treyer ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
White Matter ◽  
Gray Matter ◽  
Cerebral Perfusion ◽  
Emission Tomography ◽  
Mr Perfusion ◽  
Altman Analysis ◽  
Bland Altman Analysis ◽  
Positron Emission ◽  
The Difference

While H215O positron emission tomography (PET) is still the gold standard in the quantitative assessment of cerebral perfusion (rCBF), its technical challenge, limited availability, and radiation exposure are disadvantages of the method. Recent work demonstrated the feasibility of magnetic resonance (MR) for quantitative cerebral perfusion imaging. There remain open questions, however, especially regarding reproducibility. The main purpose of this study was to assess the accuracy and reproducibility of MR-derived flow values to those derived from H215O PET. Positron emission tomography and MR perfusion imaging was performed in 20 healthy male volunteers, who were chronic smokers, on day 1 and day 3 of a 4-day hospitalization. Subjects were randomly assigned to one of two groups, each with 10 subjects. One group was allowed to smoke as usual during the hospitalization, while the other group stopped smoking from day 2. Positron emission tomography and MR images were coregistered and rCBF was determined in two regions of interest, defined over gray matter (gm) and white matter (wm), yielding rCBFPETgm, rCBFMRgm, rCBFPETwm, and rCBFMRwm. Bland-Altman analysis was used to investigate reproducibility by assessing the difference rCBFday3 - rCBFday1 in eight continual-smoker volunteers. The analysis showed a good reproducibility for PET, but not for MR. Mean ± SD of the difference rCBFday3 - rCBFday1 in gray matter was 6.35 ± 21.06 and 0.49 ± 5.27 mL · min−1 · 100 g−1 for MR and PET, respectively; the corresponding values in white matter were 2.60 ± 15.64 and −1.14 ± 4.16 mL · min−1 · 100 g−1. The Bland-Altman analysis was also used to assess MRI and PET agreement comparing rCBF measured on day 1. The analysis demonstrated a reasonably good agreement of MR and PET in white matter (rCBFPETwm - rCBFMRwm; −0.09 ± 7.23 mL · min−1 · 100 g−1), while in gray matter a reasonable agreement was only achieved after removing vascular artifacts in the MR perfusion maps (rCBFPETgm - rCBFMRgm; −11.73 ± 14.52 mL · min−1 · 100 g−1). In line with prior work, these results demonstrate that reproducibility was overall considerably better for PET than for MR. Until reproducibility is improved and vascular artifacts are efficiently removed, MR is not suitable for reliable quantitative perfusion measurements.

Sign in / Sign up

Export Citation Format

Share Document