Assoziationsverhalten von T2-Lysozym

1964 ◽  
Vol 19 (3) ◽  
pp. 230-234 ◽  
Author(s):  
K. Wagner ◽  
W. Katz
Keyword(s):  
Molecular Weight ◽  
Diffusion Coefficients ◽  
Egg White ◽  
Molecular Weights ◽  
Egg White Lysozyme ◽  
Equilibrium Method ◽  
Ph Dependent ◽  
Reversible Association ◽  
And Diffusion

Particle-bound T 2 phage lysozyme and lysozyme from T 2 phage lysates are compared with each other by ultracentrifugation. The molecular weights determined by an equilibrium method and by sedimentation and diffusion coefficients are identical within the error of estimation.Similar to egg-white lysozyme, T 2 phage lysozyme shows a pH-dependent, reversible association.The value of the molecular weight obtained at pH 5.1 is 13 900.

scholarly journals Sedimentation-equilibrium studies on the heterogeneity of two β-lactamases

1970 ◽  
Vol 118 (3) ◽  
pp. 467-474 ◽  
Author(s):  
P. H. Lloyd ◽  
A. R. Peacocke
Keyword(s):  
Molecular Weight ◽  
Diffusion Coefficients ◽  
Minor Component ◽  
Theoretical Solution ◽  
Sedimentation Equilibrium ◽  
Equilibrium Studies ◽  
Molecular Weights ◽  
A Minor

Solutions of crystalline β-lactamase I and β-lactamase II, prepared by Kuwabara (1970), were examined in the ultracentrifuge and their sedimentation coefficients, diffusion coefficients, molecular weights and heterogeneity determined. Each sample was shown to consist of a major component comprising at least 97% of the material and a minor component of much higher molecular weight. The molecular weights of the major components were 27800 for β-lactamase I and 35600 for β-lactamase II. Emphasis is placed on a straightforward practical way of analysing the sedimentation-equilibrium results on mixtures of two macromolecular components rather than on a strict theoretical solution. Appendices describe the theory of systems at both chemical and sedimentation equilibrium and the procedure for calculating the combined distribution of two components.

MOLECULAR WEIGHT AND HYDRODYNAMIC PROPERTIES OF SODIUM ALGINATE

1954 ◽  
Vol 32 (3) ◽  
pp. 227-239 ◽  
Author(s):  
W. H. Cook ◽  
David B. Smith
Keyword(s):  
Molecular Weight ◽  
Sodium Alginate ◽  
Intrinsic Viscosity ◽  
Diffusion Coefficients ◽  
Linear Relation ◽  
Hydrodynamic Properties ◽  
Viscosity Measurements ◽  
Molecular Weights ◽  
Extension Ratio ◽  
Good Agreement

Sedimentation, diffusion, and viscosity measurements were made on five unfractionated samples of sodium alginate ranging in intrinsic viscosity from 3.1 to 17.5. Diffusion coefficients were subject to large errors and are believed to be overestimated.Though the molecular weights obtained from sedimentation–diffusion (Svedberg equation) and sedimentation – intrinsic viscosity (Perrin–Simha equations) showed good agreement and yielded values of 3 to 21 × 104, higher values (4.6 to 37 × 104) from sedimentation–viscosity (Mandelkern–Flory equation) appear to be the better estimates. A linear relation between intrinsic viscosity and molecular weight was found with a slope (Mandelkern–Flory equation values) equivalent to Km = 13.9 × 10−3. The results indicate that sodium alginate has a relatively high extension ratio.

PHYSICOCHEMICAL STUDIES OF LIGNINSULPHONATES: I. PREPARATION AND PROPERTIES OF FRACTIONATED SAMPLES

1955 ◽  
Vol 33 (10) ◽  
pp. 1477-1490 ◽  
Author(s):  
J. L. Gardon ◽  
S. G. Mason
Keyword(s):  
Molecular Weight ◽  
Diffusion Coefficients ◽  
Distribution Curve ◽  
Distilled Water ◽  
Continuous Analysis ◽  
Neutralization Equivalent ◽  
Molecular Weights ◽  
Spent Sulphite Liquor ◽  
Physicochemical Studies ◽  
Molecular Weight Distribution Curve

High molecular weight ligninsulphonates were separated from other constituents of spent sulphite liquor by a method of dialysis allowing continuous removal of the dialyzates and their replacement by distilled water. The process was controlled by continuous analysis of the residue and dialyzate. The lower molecular weight ligninsulphonates in the dialyzates were separated from the carbohydrates by precipitating their barium salts with ethanol; four fractions corresponding to different times of dialysis were prepared in this manner. The ligninsulphonates in the dialyzed liquor were separated into four additional fractions by ultrafiltration through membranes of different pore sizes. The methoxyl, sulphur, and phenolic hydroxyl contents, neutralization equivalent weights, reducing powers, ultraviolet absorption spectra, diffusion coefficients, and number-average molecular weights of the eight fractions were determined. The molecular weights of the fractions range from 3700 to 58,000 but the integral molecular weight distribution curve indicates the presence of ligninsulphonates with molecular weights as high as 100,000.

MOLECULAR WEIGHT AND HYDRODYNAMIC PROPERTIES OF SODIUM ALGINATE

1954 ◽  
Vol 32 (1) ◽  
pp. 227-239 ◽  
Author(s):  
W. H. Cook ◽  
David B. Smith
Keyword(s):  
Molecular Weight ◽  
Sodium Alginate ◽  
Intrinsic Viscosity ◽  
Diffusion Coefficients ◽  
Linear Relation ◽  
Hydrodynamic Properties ◽  
Viscosity Measurements ◽  
Molecular Weights ◽  
Extension Ratio ◽  
Good Agreement

Sedimentation, diffusion, and viscosity measurements were made on five unfractionated samples of sodium alginate ranging in intrinsic viscosity from 3.1 to 17.5. Diffusion coefficients were subject to large errors and are believed to be overestimated.Though the molecular weights obtained from sedimentation–diffusion (Svedberg equation) and sedimentation – intrinsic viscosity (Perrin–Simha equations) showed good agreement and yielded values of 3 to 21 × 104, higher values (4.6 to 37 × 104) from sedimentation–viscosity (Mandelkern–Flory equation) appear to be the better estimates. A linear relation between intrinsic viscosity and molecular weight was found with a slope (Mandelkern–Flory equation values) equivalent to Km = 13.9 × 10−3. The results indicate that sodium alginate has a relatively high extension ratio.

scholarly journals A reappraisal of some structural features of bovine heart malate dehydrogenase

1968 ◽  
Vol 109 (4) ◽  
pp. 663-668 ◽  
Author(s):  
Elizabeth Heyde ◽  
S. Ainsworth
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Malate Dehydrogenase ◽  
Acid Composition ◽  
Diffusion Coefficients ◽  
Structural Features ◽  
Similar Data ◽  
Bovine Heart ◽  
And Diffusion

1. Malate dehydrogenase of the mitochondrial type was prepared from an acetone-prepared powder of thoroughly washed minces of whole bovine heart by previously reported methods that were modified to give higher yields of the purified enzyme. 2. Determinations of the sedimentation and diffusion coefficients showed the molecular weight of the enzyme to be approx. 63000. The amino acid composition of the enzyme was also determined. Discrepancies between these data and similar data previously reported by Davies & Kun (1957) and Siegel & Englard (1962) were resolved. 3. ‘Fingerprints’ were made from tryptic digests of heat-denatured and of reduced and alkylated enzyme. These indicated that the enzyme is composed of a number of identical or similar sub-units.

scholarly journals Determination of Molecular Weights and Diffusion Coefficients in the Ultracentrifuge.

1957 ◽  
Vol 11 ◽  
pp. 1257-1270 ◽  
Author(s):  
Anders Ehrenberg ◽  
Gösta Lagerström ◽  
Mina Frydman ◽  
Lars Gunnar Sillén
Keyword(s):  
Diffusion Coefficients ◽  
Molecular Weights ◽  
And Diffusion

An Investigation of the Molecular Weight Distribution of Polymers with the Aid of the Ultracentrifuge

1957 ◽  
Vol 30 (2) ◽  
pp. 487-506
Author(s):  
S. E. Bresler ◽  
S. Y. Frenkel
Keyword(s):  
Molecular Weight ◽  
Distribution Function ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Functional Relation ◽  
Distribution Functions ◽  
Linear Polymers ◽  
Molecular Weights ◽  
Direct Experiment ◽  
And Diffusion

Abstract In the present paper a method for studying the molecular weight distribution of linear polymers, involving the following stages, is developed: 1. Fractionation of the polymer into a series of sufficiently narrow fractions and investigation of these fractions with the aid of the ultracentrifuge and diffusion. 2. Plotting the distribution functions of sedimentation constants for each fraction and subsequent summation of curves in order to build up the distribution function of the sedimentation constants for the whole polymer. 3. Discovery of a general functional relation between the sedimentation constants and molecular weights for a given series of polymer homologs and construction of the distribution function of molecular weights of the polymer. The basis of the method developed by us (method of equivalent Gaussian distributions) was confirmed by direct experiment. Application of this method of investigation to a number of industrial polymers will be described in the next paper.

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