Strahleninduzierte Aggregatbildung von Proteinen: Bindung von Aminosäuren an Myoglobin / Radiation-Induced Aggregation of Proteins: Binding of Amino Acids to Myoglobin

1978 ◽  
Vol 33 (3-4) ◽  
pp. 203-209 ◽  
Author(s):  
Vera Jakubick ◽  
Henry Delincée
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Molecule ◽  
Reactive Species ◽  
Radiation Induced ◽  
Induced Aggregation ◽  
Sulfur Containing ◽  
Tryptic Hydrolysis ◽  
Radioactive Spot

When myoglobin is irradiated in the presence of amino acids, the most radiation-reactive species, like the aromatic and sulfur-containing amino acids, will bind preferentially to the protein. The radiation-induced binding is strongly dependent on the concentration of protein and amino acid. Subsequent to irradiation of myoglobin in the presence of radioactively labelled tryptophan followed by tryptic hydrolysis, only a single radioactive spot was detected on the fingerprint. The binding of amino acids is thus not randomly distributed over the protein molecule but occurs at specific reactive sites.

Radiation-induced reductive modifications of sulfur-containing amino acids within peptides and proteins

2011 ◽  
Vol 74 (11) ◽  
pp. 2264-2273 ◽  
Author(s):  
Chryssostomos Chatgilialoglu ◽  
Carla Ferreri ◽  
Armida Torreggiani ◽  
Anna Maria Salzano ◽  
Giovanni Renzone ◽  
...  
Keyword(s):  
Amino Acids ◽  
Radiation Induced ◽  
Sulfur Containing

Novel amino acid-based ionic liquid analogues: neutral hydroxylic and sulfur-containing amino acids

2016 ◽  
Vol 11 (5) ◽  
pp. 683-694 ◽  
Author(s):  
Farouq S. Mjalli ◽  
Rashid Al-Hajri ◽  
Ala'a Al-Muhtaseb ◽  
Omar Ahmed ◽  
Maddela Nagaraju
Keyword(s):  
Amino Acids ◽  
Ionic Liquid ◽  
Amino Acid ◽  
Sulfur Containing

Species and varietal differences in the proteins of rapeseed

1969 ◽  
Vol 47 (5) ◽  
pp. 679-685 ◽  
Author(s):  
A. J. Finlayson ◽  
R. S. Bhatty ◽  
C. M. Christ
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Brassica Napus ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Brassica Campestris ◽  
Sulfur Deficiency ◽  
Brassica Napus L ◽  
Sulfur Containing

Two proteins, previously described by the authors as BI (S020, buffer 12 S) and AIVS (S020, w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.

scholarly journals Use of Food Combinatorics in the Vegetable Dishes Development of the Improved Amino Acid Composition

2021 ◽  
Vol 4 (1) ◽  
pp. 56-72
Author(s):  
Mykhailo Peresichnyi ◽  
Svitlana Peresichna ◽  
Anna Sobko
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Wheat Germ ◽  
Raw Materials ◽  
Soy Flour ◽  
Food Ingredients ◽  
Biological Value ◽  
Sulfur Containing

The topicality. To date, the structure of the population’s diet has significant deviations from the formula of a balanced diet in terms of protein intake, including sulfur-containing, which causes the formation of risk factors for the development alimentary and alimentary-dependent diseases. For the prevention of diseases caused by protein deficiency, it is promising to increase its content in vegetable dishes due to the integrated use of raw materials and dietary supplements of high biological value. It is advisable to use food combinatorics in the innovative technologies development for vegetable dishes of improved amino acid composition. The purpose of the study is food combinatorics of amino acid composition of potato croquettes with soy flour, wheat germ; natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in terms of content and balance of essential and substitute amino acids and the degree of their assimilation by the human body. Research methods. Physicochemical, mathematical and statistical methods of experimental data processing with the use of information technologies, methods of ion exchange liquid column chromatography and qualimetric methods have been used. Results. Research is aimed at the use of food combinatorics and scientific substantiation of the amino acid composition of potato croquettes with the use of protein vegetable and non-fish water raw materials and dietary supplements. As a result of scientific researches the food combinatorics at development of technology of vegetable dishes has been carried out, the amino acid structure has been proved and experimentally generalized, the amino acid score and balance of sulfur-containing amino acids in the complex use of potato mass with wheat germ, soy flour, natural shrimp powder “Rieber Food Ingredients” and lentil-spirulina filling in culinary products have been analyzed. The social effect of fuller use of vegetable, non-fish water raw materials, expansion of the range of vegetable dishes with improved amino acid composition and consumer properties of potato dishes in restaurants, preservation and protection of public health has been confirmed. Conclusions and discussions. Food combinatorics was carried out during the potato croquet technology development with the use of protein vegetable and non-fish water raw materials, which contributed to the improvement of the amino acid composition of vegetable dishes and, in particular, to the increase of their biological value.

scholarly journals Interrogation of the Perturbed Gut Microbiota in Gouty Arthritis Patients Through in silico Metabolic Modeling

2020 ◽  
Author(s):  
Michael A. Henson
Keyword(s):  
Amino Acids ◽  
Uric Acid ◽  
Amino Acid ◽  
Gut Microbiota ◽  
In Silico ◽  
Gouty Arthritis ◽  
Metabolic Modeling ◽  
Patient Specific ◽  
Surprising Result ◽  
Sulfur Containing

Recent studies have shown perturbed gut microbiota associated with gouty arthritis, a metabolic disease in which an imbalance between uric acid production and excretion leads to the deposition of uric acid crystals in joints. To mechanistically investigate altered microbiota metabolism in gout disease, 16S rRNA gene amplicon sequence data from stool samples of gout patients and healthy controls were computationally analyzed through bacterial community metabolic modeling. Patient-specific models were used to cluster samples according to their metabolic capabilities and to generate statistically significant partitioning of the samples into a Bacteroides-dominated, high gout cluster and a Faecalibacterium-elevated, low gout cluster. The high gout cluster samples were predicted to allow elevated synthesis of the amino acids D-alanine and L-alanine and byproducts of branched-chain amino acid catabolism, while the low gout cluster samples allowed higher production of butyrate, the sulfur-containing amino acids L-cysteine and L-methionine and the L-cysteine catabolic product H2S. The models predicted an important role for metabolite crossfeeding, including the exchange of acetate, D-lactate and succinate from Bacteroides to Faecalibacterium to allow higher butyrate production differences than would be expected based on taxa abundances in the two clusters. The surprising result that the high gout cluster could underproduce H2S despite having a higher abundance of H2S-synthesizing bacteria was rationalized by reduced L-cysteine production from Faecalibacterium in this cluster. Model predictions were not substantially altered by constraining uptake rates with different in silico diets, suggesting that sulfur-containing amino acid metabolism generally and H2S more specifically could be novel gout disease markers.

scholarly journals Synthesis and Characterization of Various Amino Acid Derived Thiohydantoins

Proceedings ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 37
Author(s):  
Petar Stanić ◽  
Marija Živković ◽  
Biljana Šmit
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Allyl Isothiocyanate ◽  
Structural Diversity ◽  
Biologically Active ◽  
Reaction Conditions ◽  
X Ray ◽  
X Ray Crystallography ◽  
Sulfur Containing

Hydantoins and their sulfur containing analogues, thiohydantoins, are cyclic ureides that have attracted huge attention ever since their discovery. Most of them are biologically active compounds and several points of structural diversity have made them very synthetically attractive. Although substituents can be introduced to the hydantoin nucleus, most substituted hydantoins are synthesized from substrates already containing these groups, while forming the hydantoin nucleus. This is a common route to the synthesis of hydantoins and one of them is employed in this study. A series of 3-allyl-2-thiohydantoins is synthesized from various α-amino acids in a reaction with allyl isothiocyanate. The substitution of the acquired thiohydantoin depends on the structure of the starting α-amino acid. The residual group of the α-amino acid becomes the substituent at the C5-position, while N-monosubstituted amino acids give rise to a substituent in the N1-position. The reaction is carried out in a two-step process and the reaction conditions generally depend on the nature of the amino acid itself. All thiohydantoins are obtained in a good yield and fully characterized by NMR and IR spectroscopy, as well as X-ray crystallography.

Changes in plasma amino acid patterns and wool growth in response to abomasal injections of amino acids during late pregnancy and early lactation

1993 ◽  
Vol 44 (5) ◽  
pp. 959 ◽  
Author(s):  
CA Stewart ◽  
DG Masters ◽  
IH Williams ◽  
PJ Connell
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Late Pregnancy ◽  
Early Lactation ◽  
Growth Responses ◽  
Specific Amino Acid ◽  
Protein Deposition ◽  
Wool Growth ◽  
Limiting Amino Acids ◽  
Sulfur Containing

Provision of sulfur-containing amino acids into the abomasum has increased wool production in non-reproducing sheep and, on the basis of such studies, they have generally been considered as the primary limiting nutrients for wool growth. The specific amino acid needs of reproducing ewes were investigated in the current study. Three groups of pregnant ewes were fitted with temporary abomasal fistulae. For the last 3-4 weeks of pregnancy and the first 3 weeks of lactation, solutions containing sodium hydroxide (as a carrier) or methionine or a mixture of valine, arginine, lysine and threonine (VALT) were injected into the abomasum twice per day. The injection of methionine did not increase protein deposition during pregnancy; this was indicated by the failure of the treatment to lower the concentration of other amino acids in plasma, or increase liveweight, lamb birth weight or wool growth. Responses in early lactation were different with the methionine injected sheep having a lower concentration of free lysine, valine, alanine and serine in plasma, indicating an increased rate of protein deposition, and a faster growth rate in the ewes. Injections of VALT caused a decrease in the concentration of cystine and methionine in plasma and depressed wool growth and tensile strength. Theresults indicate that a lack of methionine was not limiting protein deposition during late pregnancy, but may have been during early lactation. Valine, arginine, lysine and threonine were not the primary limiting amino acids in late pregnancy or early lactation.

Synthetic studies of novel ninhydrin analogs

2001 ◽  
Vol 79 (11) ◽  
pp. 1632-1654 ◽  
Author(s):  
Richard R Hark ◽  
Diane B Hauze ◽  
Olga Petrovskaia ◽  
Madeleine M Joullié
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Cross Coupling ◽  
Coupling Reactions ◽  
Latent Fingerprints ◽  
Cross Coupling Reactions ◽  
Essential Tool ◽  
Porous Surfaces ◽  
Synthetic Studies ◽  
Sulfur Containing

Ninhydrin is an essential tool in the analysis of amino acids, peptides, and proteins, and the preferred reagent for the detection of latent fingerprints on porous surfaces. The goal of this investigation was to prepare ninhydrin analogs with enhanced chromogenic and fluorogenic properties. Target compounds included structures with extended conjugation and (or) with the presence of sulfur-containing moieties. We have devised general convergent routes for novel heterocyclic and aryl-substituted ninhydrin analogs for use as reagents for amino acid detection.Key words: ninhydrin analogs, synthesis, ketals, Suzuki cross-coupling reactions, Stille cross-coupling reactions.

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