In vivo und in vitro Hydroxylierung von Androgenen in Ratten nach Leberschädigung und Nahrungskarenz / In vivo and in vitro hydroxylation of testosterone and dihydrotestosterone in rats after liver damage and starvation

1980 ◽  
Vol 35 (11-12) ◽  
pp. 995-998
Author(s):  
Martin Wenzel ◽  
Ursula Lemm
Keyword(s):  
Liver Damage ◽  
Experimental Liver ◽  
Experimental Liver Damage ◽  
T Testosterone

The in vivo C-2-hydroxylation of testosterone and 5-dihydrotestosterone in rats was measured by labilisation of tritium as HTO after injection of [1α, 2α-T]testosterone or [1 α, 2α-T]5α-dihy- drotestosterone (radiospirometry). After experimental liver damage caused by CCl4 or after starvation for 60 h the hyroxylation of the androgens decreased. Similar results were gained by measuring the C-2-hydroxylation of testosterone, 5 α-dihydrotestosterone and estradiol.

Hepatoprotective Effects of Hovenia dulcis Fruit on Ethanol-Induced Liver Damage in vitro and in vivo

2009 ◽  
Vol 38 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Yang-Hee You ◽  
Kuk-Yung Jung ◽  
Yoo-Hyun Lee ◽  
Woo-Jin Jun ◽  
Boo-Yong Lee
Keyword(s):  
Liver Damage ◽  
Hepatoprotective Effects ◽  
Hovenia Dulcis

scholarly journals Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage

2008 ◽  
Vol 52 (No. 3) ◽  
pp. 121-129
Author(s):  
D. Kosakova ◽  
P. Scheer ◽  
J. Lata ◽  
J. Doubek
Keyword(s):  
Escherichia Coli ◽  
Lymph Nodes ◽  
Liver Damage ◽  
Weight Ratio ◽  
Coliform Bacteria ◽  
Control Group ◽  
Significant Difference ◽  
And Control ◽  
Experimental Liver ◽  
Experimental Liver Damage

The aim of the study was evaluate the influence of the probiotic <i>Escherichia coli</i> Nissle 1917 strain (Mutaflor&reg; suspension, Ardeypharm GmbH, Herdecke, Germany) on bacterial translocation in cases of liver damage, damage to the intestinal mucosa, potential portal hypertension associated with possible development of oesophageal varices and on the bacterial population of the intestine during chronic experimental liver damage in the laboratory rat. Rats with liver damage induced by thioacetamide were divided into an experimental and control group. Experimental and control animals were applied Mutaflor and saline, respectively. Samples of blood, liver, lymph nodes and caecum for microbiological examination, of liver, duodenum and oesophagus for histological examination and of spleen for weight evaluation were collected. There were no significant differences between both groups of animals in the qualitative proportion of <i>Staphylococcus</i> spp., <i>Enterococcus</i> spp. and <i>Proteus</i> spp. cultured from the lymph nodes, blood and liver. The quantitative culture results on <i>Enterococcus</i> spp. in the caecum, liver and lymph nodes showed no significant differences between both groups. There was a significant difference between the experimental and control group in the counts of coliform bacteria. No significant differences between both groups were found in the overall damage score of the liver, duodenum and oesophagus. There were no differences in the spleen to body weight ratio of both groups. The application of Mutaflor&reg; suspension for eight days had no recognisable effect diminishing the selected complications of chronic liver damage caused by the administration of TAA to laboratory rats.

Hepatoprotective effect of ethanolic extract ofEclipta alba on experimental liver damage in rats and mice

1993 ◽  
Vol 7 (2) ◽  
pp. 154-158 ◽  
Author(s):  
B. Singh ◽  
A. K. Saxena ◽  
B. K. Chandan ◽  
S. G. Agarwal ◽  
M. S. Bhatia ◽  
...  
Keyword(s):  
Liver Damage ◽  
Ethanolic Extract ◽  
Hepatoprotective Effect ◽  
Rats And Mice ◽  
Experimental Liver ◽  
Experimental Liver Damage

Effects of testosterone and prolactin on steroidogenesis in post-ovulatory cumuli oophori and on in vitro oocyte fertilisation in the rat

2017 ◽  
Vol 29 (2) ◽  
pp. 406 ◽  
Author(s):  
Agnieszka Starowicz ◽  
Jerzy Galas ◽  
Małgorzata Duda ◽  
Zbigniew Tabarowski ◽  
Maria Szołtys
Keyword(s):  
In Vitro Fertilisation ◽  
Fertilisation Rate ◽  
In Vitro Effects ◽  
Very High ◽  
Secretion Rates ◽  
T Testosterone

The main objective of these studies was to determine the in vitro effects of prolactin (PRL) and testosterone (T) on steroidogenic function in post-ovulatory cumuli oophori containing unfertilised (ufCOCs) or fertilised (fCOCs) oocytes and to determine the differences between ufCOCs and fCOCs. In vivo, progesterone (P4) content was distinctly higher in isolated ampullae containing ufCOCs than in those containing fCOCs. Moreover, the expression of androgen (ARs) and prolactin (PRL-Rs) receptors was distinctly higher in ufCOCs than in fCOCs. Also, in vitro P4 profiles were generally higher in incubated ufCOCs, which had very high secretion rates of this steroid, especially after treatment with PRL+T. Testosterone significantly increased P4 levels only in incubated fCOCs, while the anti-androgen dihydroxyflutamide (2-Hf) markedly decreased P4 levels in both ufCOCs and fCOCs. Among post-incubation ufCOCs fertilised in vitro, the highest fertilisation rate was observed for oocytes in ufCOCs exposed to PRL+T, while those incubated with 2-Hf or T+2-Hf were not fertilisable. These studies establish differences in steroidogenic function and expression of ARs and PRL-Rs between post-ovulatory ufCOCs and fCOCs, with higher concentrations of P4 being observed in the microenvironment of ufCOCs. PRL+T stimulated P4 production by ufCOCs and increased in vitro fertilisation rate.

scholarly journals BMP-9 Modulates the Hepatic Responses to LPS

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 617
Author(s):  
Haristi Gaitantzi ◽  
Julius Karch ◽  
Lena Germann ◽  
Chen Cai ◽  
Vanessa Rausch ◽  
...  
Keyword(s):  
Liver Damage ◽  
Human Liver ◽  
Liver Sinusoidal Endothelial Cells ◽  
Inflammatory Reactions ◽  
Morphogenetic Protein ◽  
Liver Fibrogenesis ◽  
Nfkb Pathway ◽  
Pre Treatment

It was previously shown that Bone Morphogenetic Protein (BMP)-9 is constitutively produced and secreted by hepatic stellate cells (HSC). Upon acute liver damage, BMP-9 expression is transiently down-regulated and blocking BMP-9 under conditions of chronic damage ameliorated liver fibrogenesis in C57BL/6 mice. Thereby, BMP-9 acted as a pro-fibrogenic cytokine in the liver but without directly activating isolated HSC in vitro. Lipopolysaccharide (LPS), an endotoxin derived from the membrane of Gram-negative bacteria in the gut, is known to be essential in the pathogenesis of diverse kinds of liver diseases. The aim of the present project was therefore to investigate how high levels of BMP-9 in the context of LPS signalling might result in enhanced liver damage. For this purpose, we stimulated human liver sinusoidal endothelial cells (LSEC) with LPS and incubated primary human liver myofibroblasts (MF) with the conditioned medium of these cells. We found that LPS led to the secretion of factors from LSEC that upregulate BMP-9 expression in MF. At least one of these BMP-9 enhancing factors was defined to be IL-6. High BMP-9 in turn, especially in combination with LPS stimulation, induced the expression of certain capillarization markers in LSEC and enhanced the LPS-mediated induction of pro-inflammatory cytokines in primary human macrophages. In LSEC, pre-treatment with BMP-9 reduced the LPS-mediated activation of the NfkB pathway, whereas in macrophages, LPS partially inhibited the BMP-9/Smad-1 signaling cascade. In vivo, in mice, BMP-9 led to the enhanced presence of F4/80-positive cells in the liver and it modulated the LPS-mediated regulation of inflammatory mediators. In summary, our data point to BMP-9 being a complex and highly dynamic modulator of hepatic responses to LPS: Initial effects of LPS on LSEC led to the upregulation of BMP-9 in MF but sustained high levels of BMP-9 in turn promote pro-inflammatory reactions of macrophages. Thereby, the spatial and timely fine-tuned presence (or absence) of BMP-9 is needed for efficient wound-healing responses in the liver.

Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo

2012 ◽  
Vol 214 (1) ◽  
pp. 69-80 ◽  
Author(s):  
Xiaoming Zhao ◽  
Xiaonan Cong ◽  
Lingli Zheng ◽  
Lina Xu ◽  
Lianhong Yin ◽  
...  
Keyword(s):  
Protective Effect ◽  
Liver Damage ◽  
Steroid Saponin

197 Hepcidin and hemojuvelin gene expression in rat liver damage: In vivo and in vitro studies

2006 ◽  
Vol 44 ◽  
pp. S81-S82
Author(s):  
N. Sheikh ◽  
K. Tron ◽  
J. Dudas ◽  
B. Saile ◽  
D. Batusic ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Liver Damage ◽  
In Vitro Studies
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