The Essential Oil of Coleonema album (Rutaceae) and of a Photomixotrophic Cell Culture Derived thereof

1990 ◽  
Vol 45 (3-4) ◽  
pp. 187-195 ◽  
Author(s):  
R. G. Berger ◽  
Z. Akkan ◽  
F. Drawert
Keyword(s):  
Cell Culture ◽  
Essential Oil ◽  
Cell Line ◽  
Nutrient Medium ◽  
Close Correlation ◽  
Callus Cultures ◽  
Intact Plant ◽  
Lower Amount ◽  
Plastid Differentiation ◽  
Oil Synthesis

Abstract The volatile shoot oil of Coleonema album was found to be composed of monoterpenes, ses­quiterpenes and phenylpropanoids. Heterotrophic and photom ixotrophic callus cultures were established from green parts of the plant. A photom ixotrophic cell line contained a heteroge­neous population of plastids and synthesized an essential oil, which differed qualitatively and quantitatively from the green parts of the intact plant. Substitution of 2,4-D and pCPA by a lower amount of NAA and high kinetin concentrations favoured the formation of essential oil. The heterotrophic callus, when grown on the same nutrient medium, was devoid of any differ­ entiated plastids and of essential oil constituents. Thus, a close correlation of light-induced plastid differentiation and essential oil synthesis was concluded.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

scholarly journals Tribological, biocompatibility, and antibiofilm properties of tungsten–germanium coating using magnetron sputtering

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Mustafa Şükrü Kurt ◽  
Mehmet Enes Arslan ◽  
Ayşenur Yazici ◽  
İlkan Mudu ◽  
Elif Arslan
Keyword(s):  
Cell Culture ◽  
Magnetron Sputtering ◽  
Cell Line ◽  
Borosilicate Glass ◽  
Chromosomal Abnormalities ◽  
Dermal Fibroblast ◽  
Fibroblast Cell ◽  
Antibiofilm Activity ◽  
Borosilicate Glasses

AbstractIn this study, borosilicate glass and 316 L stainless steel were coated with germanium (Ge) and tungsten (W) metals using the Magnetron Sputtering System. Surface structural, mechanical, and tribological properties of uncoated and coated samples were examined using SEM, X-ray diffraction (XRD), energy-dispersive spectroscopy, and tribometer. The XRD results showed that WGe2 chemical compound observed in (110) crystalline phase and exhibited a dense structure. According to the tribological analyses, the adhesion strength of the coated deposition on 316 L was obtained 32.8 N, and the mean coefficient of friction was around 0.3. Biocompatibility studies of coated metallic biomaterials were analyzed on fibroblast cell culture (Primary Dermal Fibroblast; Normal, Human, Adult (HDFa)) in vitro. Hoescht 33258 fluorescent staining was performed to investigate the cellular density and chromosomal abnormalities of the HDFa cell line on the borosilicate glasses coated with germanium–tungsten (W–Ge). Cell viabilities of HDFa cell line on each surface (W–Ge coated borosilicate glass, uncoated borosilicate glass, and cell culture plate surface) were analyzed by using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity assay. The antibiofilm activity of W–Ge coated borosilicate glass showed a significant reduction effect on Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) adherence compared to control groups. In the light of findings, tungsten and germanium, which are some of the most common industrial materials, were investigated as biocompatible and antimicrobial surface coatings and recommended as bio-implant materials for the first time.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium

Fish cell culture: Characteristics of a cell line from the silver perch,Bairdiella chrysura

In Vitro ◽  
1977 ◽  
Vol 13 (6) ◽  
pp. 389-397 ◽  
Author(s):  
J. H. Wharton ◽  
R. D. Ellender ◽  
B. L. Middlebrooks ◽  
P. K. Stocks ◽  
Adrian R. Lawler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Fish Cell ◽  
Fish Cell Culture ◽  
Culture Characteristics ◽  
A Cell

The Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine Treatment in Resistant 2D and 3D Model Triple Negative Breast Cancer Cell Line: ABCG2 Expression Data

2021 ◽  
Vol 21 ◽  
Author(s):  
Fatma Kubra Ata ◽  
Serap Yalcin
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Expression Profile ◽  
Three Dimensional ◽  
Parental Cell ◽  
Parental Cell Line ◽  
Xtt Assay ◽  
Gene And Protein Expression

Background: Chemotherapeutics have been commonly used in cancer treatment. Objective: In this study, the effects of Cisplatin, 5-fluorouracil, Irinotecan, and Gemcitabine have been evaluated on two-dimensional (2D) (sensitive and resistance) cell lines and three dimensional (3D) spheroid structure of MDA-MB-231. The 2D cell culture lacks a natural tissue-like structural so, using 3D cell culture has an important role in the development of effective drug testing models. Furthermore, we analyzed the ATP Binding Cassette Subfamily G Member 2 (ABCG2) gene and protein expression profile in this study. We aimed to establish a 3D breast cancer model that can mimic the in vivo 3D breast cancer microenvironment. Methods: The 3D spheroid structures were multiplied (globally) using the three-dimensional hanging drop method. The cultures of the parental cell line MDA-MB-231 served as the controls. After adding the drugs in different amounts we observed a clear and well-differentiated spheroid formation for 24 h. The viability and proliferation capacity of 2D (sensitive and resistant) cell lines and 3D spheroid cell treatment were assessed by the XTT assay. Results: Cisplatin, Irinotecan, 5-Fu, and Gemcitabine-resistant MDA-MB-231 cells were observed to begin to disintegrate in a three-dimensional clustered structure at 24 hours. Additionally, RT-PCR and protein assay showed overexpression of ABCG2 when compared to the parental cell line. Moreover, MDA-MB-231 cells grown in 3D showed decreased sensitivity to chemotherapeutics treatment. Conclusion: More resistance to chemotherapeutics and altered gene expression profile was shown in 3D cell cultures when compared with the 2D cells. These results might play an important role to evaluate the efficacy of anticancer drugs, explore mechanisms of MDR in the 3D spheroid forms.

Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT-29

2009 ◽  
Vol 53 (10) ◽  
pp. 1226-1236 ◽  
Author(s):  
Phillip Bellion ◽  
Melanie Olk ◽  
Frank Will ◽  
Helmut Dietrich ◽  
Matthias Baum ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Culture ◽  
Cell Line ◽  
Culture Media ◽  
Cell Culture Media ◽  
Colon Cell ◽  
Colon Cell Line ◽  
Ht 29
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