Lupeol Alkanoates in Brazilian Propolis

2002 ◽  
Vol 57 (7-8) ◽  
pp. 721-726 ◽  
Author(s):  
Alberto S. Pereira ◽  
Evandro A. Nascimento ◽  
Francisco R. de Aquino Neto
Keyword(s):  
Mass Spectrometry ◽  
Native Plants ◽  
Analytical Tool ◽  
Material Sources ◽  
Minas Gerais State ◽  
Powerful Analytical Tool ◽  
High Resolution Gas Chromatography ◽  
Brazilian Propolis ◽  
First Time ◽  
The City

High temperature high resolution gas chromatography coupled to mass spectrometry (HTHRGC- MS) isa powerful analytical tool. In this work we applied this technique to the study of crude extracts of propolis collected near the city of Uberlândia - Minas Gerais State. Eucalyptus trees and native plants from “cerrado” (savannah) were the material sources disposable for the Apis mellifera bees. A lot of known propolis constituents were identified, however, several high molecular weight compounds including lupeol alkanoates were identified for first time in propolis.

Pentacyclic Triterpenoid Alkanoates in Propolis

1999 ◽  
Vol 54 (12) ◽  
pp. 1115-1118
Author(s):  
Alberto dos Santos Pereira ◽  
Joaquim Fernando Mendes da Silva ◽  
Renata Kiltzke ◽  
Jari Nobrega Cardoso ◽  
Francisco Radler de Aquino Neto
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Molecular Mass ◽  
Pentacyclic Triterpenoid ◽  
State Identification ◽  
Minas Gerais State ◽  
Hexane Extracts ◽  
High Resolution Gas Chromatography ◽  
Brazilian Propolis ◽  
First Time

A series of high molecular mass pentacyclic terpenoid wax esters (TTFAE) were characterized for the first time in hexane extracts of Brazilian propolis (Carangola, Minas Gerais State). Identification was by HT-HRGC (High Temperature High Resolution Gas Chromatography) and HT-HRGC coupled to mass spectrometry (HT-HRGC-MS).

scholarly journals Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

2020 ◽  
Vol 21 (20) ◽  
pp. 7465 ◽  
Author(s):  
Vasilisa V. Krasitskaya ◽  
Eugenia E. Bashmakova ◽  
Ludmila A. Frank
Keyword(s):  
Analytical Methods ◽  
Analytical Tool ◽  
Biological Research ◽  
Research Areas ◽  
Enzyme Substrate ◽  
Highly Sensitive ◽  
Powerful Analytical Tool ◽  
Fundamental Research ◽  
Common Group ◽  
First Time

The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases.

Mass spectrometry as a powerful analytical tool for the characterization of indoor airborne microplastics and nanoplastics

2021 ◽  
Author(s):  
Milica Velimirovic ◽  
Kristof Tirez ◽  
Sandra Verstraelen ◽  
Evelien Frijns ◽  
Sylvie Remy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Particle Size ◽  
Analytical Methods ◽  
Analytical Tool ◽  
Chemical Identification ◽  
Size Determination ◽  
Particle Size Determination ◽  
Powerful Analytical Tool ◽  
Identification And Quantification

Development of analytical methods for the characterization (particle size determination, chemical identification, and quantification) of the low µm-range microplastics (MPs; 1-10 µm) and nanoplastics (NPs; 1 nm-1 µm) in air...

scholarly journals Environmental Factors do not Affect the Phenolic Profile of Hypericum perforatum Growing Wild in Bosnia and Herzegovina

2017 ◽  
Vol 12 (9) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Sanja Ćavar Zeljković ◽  
Erna Karalija ◽  
Adisa Parić ◽  
Edina Muratović ◽  
Petr Tarkowski
Keyword(s):  
Environmental Factors ◽  
Bosnia And Herzegovina ◽  
Hypericum Perforatum ◽  
Analytical Tool ◽  
Phenolic Profile ◽  
Glycosidic Bonds ◽  
Fractionation Procedure ◽  
Powerful Analytical Tool ◽  
Hypericum Perforatum L ◽  
First Time

Using appropriate isolation and fractionation procedure, and UHPLC-MS/MS as powerful analytical tool, comprehensive phenolic acid profile of leaves and flowers of Hypericum perforatum L., growing wild in Bosnia and Herzegovina, has been analyzed for the first time. The phenolic acids were isolated from plant tissue, liberated from esters and glycosidic bonds, and then analyzed as free acids. Results show that free forms of caffeic and chlorogenic acid were the most abundant. Generally, the content of acids is higher in flowers than in leaves, but environmental factors do not significantly influence chemical composition of this medicinal plant.

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