Histone H1 Interacts Preferentially with DNA Fragments Containing a Cisplatin- Induced 1,2-Intrastrand Cross-Link

2007 ◽  
Vol 62 (11-12) ◽  
pp. 905-908 ◽  
Author(s):  
Julia N. Yaneva ◽  
Elena G. Paneva ◽  
Siyka I. Zacharieva ◽  
Jordanka Zlatanova
Keyword(s):  
Experimental Data ◽  
Anticancer Agents ◽  
Histone H1 ◽  
Recent Experimental Data ◽  
Band Shift ◽  
Single Target ◽  
Shift Analysis ◽  
Modified Dna ◽  
Cross Links ◽  
Dna Fragment

Cisplatin [cis-diamminedichloroplatinum(II) or cis- DDP], but not its stereoisomer transplatin, is suggested to be among the most powerful anticancer agents. It is believed that its therapeutic activity results from its interaction with DNA forming intra- and interstrand crosslinks. During our earlier investigations, we have observed a prominent preference of the linker histone H1 for binding to cis-platinated DNA (containing several different cross-links along the DNA fragment) compared with unmodified or transplatin-modified DNA. This report presents our recent experimental data obtained by band-shift analysis on the binding of H1 to a cisplatin-modified synthetic 34 bp DNA fragment containing a single target d(GG/CC) for 1,2 cis-intra-platination. Results obtained with another nuclear protein with similar DNA-binding properties, HMGB1, are also presented. The experimental data throw light on the precise preference of histone H1 for binding to different types of cisplatin-created cross-links in DNA.

Fusion and Breakup Reactions of 17S + 208Pb and 15C + 232ThHalo Nuclei Systems

2015 ◽  
Vol 11 (2) ◽  
pp. 2972-2978
Author(s):  
Fouad A. Majeed ◽  
Yousif A. Abdul-Hussien
Keyword(s):  
Experimental Data ◽  
Cross Section ◽  
Fusion Reaction ◽  
Fusion Cross Section ◽  
Reaction Cross ◽  
Recent Experimental Data ◽  
Barrier Distribution ◽  
Coupled Channel ◽  
Total Fusion ◽  
Full Quantum

In this study the calculations of the total fusion reaction cross section have been performed for fusion reaction systems 17F + 208Pb and 15C + 232Th which involving halo nuclei by using a semiclassical approach.The semiclassical treatment is comprising the WKB approximation to describe the relative motion between target and projectile nuclei, and Continuum Discretized Coupled Channel (CDCC) method to describe the intrinsic motion for both target and projectile nuclei. For the same of comparsion a full quantum mechanical clacualtions have been preforemd using the (CCFULL) code. Our theorticalrestuls are compared with the full quantum mechaincialcalcuations and with the recent experimental data for the total fusion reaction  checking the stability of the distancesThe coupled channel calculations of the total fusion cross section σfus, and the fusion barrier distribution Dfus. The comparsion with experiment proves that the semiclassiacl approach adopted in the present work reproduce the experimental data better that the full quantal mechanical calcautions. 

scholarly journals Regulation of the nfsA Gene in Escherichia coli by SoxS

2002 ◽  
Vol 184 (1) ◽  
pp. 51-58 ◽  
Author(s):  
E. Suzanne Paterson ◽  
Sherri E. Boucher ◽  
I. B. Lambert
Keyword(s):  
Escherichia Coli ◽  
Promoter Sequence ◽  
Open Reading Frame ◽  
Base Sequence ◽  
Start Site ◽  
Transcription Start ◽  
Band Shift ◽  
Reading Frame ◽  
Shift Analysis ◽  
Small Open Reading Frame

ABSTRACT In Escherichia coli, the response to oxidative stress due to elevated levels of superoxide is mediated, in part, by the soxRS regulon. One member of the soxRS regulon, nfsA, encodes the major oxygen-insensitive nitroreductase in Escherichia coli which catalyzes the reduction of nitroaromatic and nitroheterocyclic compounds by NADPH. In this study we investigate the regulation of nfsA in response to the superoxide generating compound paraquat. The transcription start site (TSS) of nfsA was located upstream of the ybjC gene, a small open reading frame of unknown function located directly upstream of nfsA, suggesting that these two genes form an operon. The activity of the promoter associated with this TSS was confirmed with lacZ fusions and was shown to be inducible by paraquat. Footprinting and band shift analysis showed that purified His-tagged SoxS protein binds to a 20-base sequence 10 bases upstream of the −35 promoter sequence in the forward orientation, suggesting that the ybjC-nfsA promoter is a class I SoxS-dependent promoter.

scholarly journals Mobility Enhancement in Square Quantum Wells: Symmetric Modulation of the Envelop Wave Function

2010 ◽  
Vol 20 (3) ◽  
pp. 193
Author(s):  
Doan Nhat Quang ◽  
Nguyen Huyen Tung ◽  
Nguyen Trung Hong ◽  
Tran Thi Hai
Keyword(s):  
Experimental Data ◽  
Wave Function ◽  
Low Temperature ◽  
Quantum Wells ◽  
Quantum Transport ◽  
Variational Approach ◽  
Theoretical Study ◽  
Recent Experimental Data ◽  
Carrier Distribution ◽  
Analytic Expressions

We present a theoretical study of the effects from symmetric modulation of the envelop wave function on quantum transport in square quantum wells (QWs). Within the variational approach we obtain analytic expressions for the carrier distribution and their scattering in symmetric two-side doped square QWs. Roughness-induced scattering are found significantly weaker than those in the asymmetric one-side doped counterpart. Thus, we propose symmetric modulation of the wave function as an efficient method for enhancement of the roughness-limited QW mobility. Our theory is able to well reproduce the recent experimental data about low-temperature transport of electrons and holes in two-side doped square QWs, e.g., the mobility dependence on the channel width, which have not been explained so far.

scholarly journals Effect of Solar Modulation on the Low Energy Sea Level Muon Spectrum Near the Geomagnetic Equator

1978 ◽  
Vol 31 (5) ◽  
pp. 451 ◽  
Author(s):  
DP Bhattacharyya
Keyword(s):  
Experimental Data ◽  
Sea Level ◽  
Low Energy ◽  
Solar Modulation ◽  
Recent Experimental Data ◽  
Geomagnetic Equator ◽  
Muon Spectrum ◽  
Muon Intensity ◽  
Theoretical Results

A study is made of the influence of long-term solar modulation on the low energy sea level muon spectrum near the geomagnetic equator. Recent experimental data are compared with theoretical results calculated from the phenomenological model of Allkofer and Dau. It is suggested that the observed enhancement in the muon intensity is mainly due to a shift in the solar potential.

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