scholarly journals Anticoagulant Effects of Glycosaminoglycan Extracted from Fish Scales

2018 ◽  
Vol 3 (2) ◽  
pp. 72-77 ◽  
Author(s):  
Katayoon Karimzadeh
Keyword(s):  
Anticoagulant Activity ◽  
Cetylpyridinium Chloride ◽  
Activated Partial Thromboplastin Time ◽  
Bioactive Molecules ◽  
Chemical Characteristics ◽  
Thrombin Time ◽  
Fish Scales ◽  
Coagulation Time ◽  
Plasma Coagulation ◽  
Anticoagulant Property

Background: Fisheries wastes are the unique sources of natural products which structural and chemical characteristics of their extracted compounds are different from those of terrestrial animals. They are known as a rich source of bioactive molecules, including collagen and glycosaminoglycan (GAGs).In the present study, we extracted and analysed anticoagulant activity of glycosaminoglycan from fish (Rutilus frisii kuum) scales Materials and Methods: The glycosaminoglycan compounds were extracted using cationic salt of cetylpyridinium chloride (CPC) and Fourier transform infrared spectroscopy (FTIR) spectrum was used to identify and compare the structure of the extracted glycosaminoglycan with heparin. Anticoagulant property of extracted glycosaminoglycan was measured by prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT) on human blood at three concentrations of 50, 100, and 200 µg/ml. Results: The value of the extracted glycosaminoglycan was estimated approximately 23.8 mg/g of dry tissue.FTIR analysis results confirmed the presence of heparin-like compounds in fish scales glycosaminoglycan. Human Plasma coagulation time increased significantly with extracted GAGs concentration incriminated. Since at a concentration of 200 µg/ml, coagulation time as aPTT was 4.3 times that of control and coagulation time was prolonged about 138.6 seconds. Conclusions: The results of the current study showed that the glycosaminoglycan extracted from fish scales had valuable anticoagulant property compared to synthetic anticoagulant compounds such as heparin.

scholarly journals Antihypertensive and anticoagulant properties of glycosaminoglycans extracted from the sturgeon (Acipenser persicus) cartilage

2018 ◽  
Vol 31 (4) ◽  
pp. 163-169
Author(s):  
Katayoon Karimzadeh
Keyword(s):  
Anticoagulant Activity ◽  
Cetylpyridinium Chloride ◽  
Dry Weight ◽  
Biological Properties ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Chloride Salt ◽  
Thrombin Time ◽  
Acipenser Persicus ◽  
Ace Inhibitory

Abstract Large amounts of valuable waste are produced during sea food processing. This has a great potential for conversion to biologically active proteins and polysaccharides. Among these compounds, sulfated polysaccharides have been considered due to their many biological properties. The present work was conducted to study anticoagulant activities and angiotensin-I converting enzyme (ACE) inhibitory effects of glycosaminoglycans (GAGs) extracted from the cartilage of sturgeon (Acipenser persicus). The enzymatic extraction of sturgeon cartilage was performed in the presence of cetylpyridinium chloride salt. The structure was characterized via electron microscope and Fourier transform infrared spectroscopy (FTIR) analysis. Herein, ACE inhibitory and anticoagulant properties of extracted GAGs were determined. The amount of GAGs was 6.8±1.3% of cartilage dry weight. GAGs showed good activity in ACE inhibitory – with a highest level of 85.7%. The derived anticoagulant activity indexes, APPT (activated partial thromboplastin time) and TT (Thrombin time) of the extracted polysaccharide showed a prolonging of clotting time, compare to control. The results of this study revealed that the cartilage extracted GAGs possess promising ACE inhibitory properties and anticoagulant effects. Thus, the product can be substituted for blood reducing drugs and antithrombotic agents at least in laboratory conditions.

scholarly journals Antioxidant, anti-inflammatory and anticoagulant activities of three Thymus species grown in southeastern Morocco

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Abdelbassat Hmidani ◽  
Eimad Dine Tariq Bouhlali ◽  
Tarik Khouya ◽  
Mhamed Ramchoun ◽  
Younes Filali-Zegzouti ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Activated Partial Thromboplastin Time ◽  
Dependent Manner ◽  
Thrombin Time ◽  
Thymus Zygis ◽  
Wide Range ◽  
Thymus Satureioides ◽  
Anti Inflammatory ◽  
Dose Dependent

Abstract Background Thyme has been used for centuries in southeastern Morocco to treat a wide range of diseases such as inflammation disorders. The aim of the current study is to examine and to compare in vitro the anti-inflammatory, antioxidant, and anticoagulant activities of three thyme species grown in southeastern Morocco. Results Data showed that all studied species possess an important antioxidant activity: Thymus atlanticus (IC50 = 16.59 μg/mL), Thymus zygis (IC50 = 15.43 μg/mL), and Thymus satureioides (IC50 = 14.65 μg/mL). Concerning the anti-inflammatory activity, the highest effect was depicted in Thymus atlanticus followed by Thymus zygis and Thymus satureioides. With regard to the anticoagulant activity, the aqueous extract of these species prolongs activated partial thromboplastin time, prothrombin time, and thrombin time significantly (p < 0.05) in a dose-dependent manner. Conclusion Our results provide evidence that thymus extract exhibits marked antioxidant, anticoagulant, and anti-inflammatory effects, thus justifying the popular uses of these plants to treat some inflammatory and cardiovascular illnesses.

scholarly journals Monoclonal IgG anticoagulants delaying fibrin aggregation in two patients with systemic lupus erythematosus (SLE)

Blood ◽  
1978 ◽  
Vol 52 (5) ◽  
pp. 1037-1046
Author(s):  
DK Galanakis ◽  
EM Ginzler ◽  
SM Fikrig
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Self Assembly ◽  
Anticoagulant Activity ◽  
Rabbit Serum ◽  
Distinct Region ◽  
Thrombin Time ◽  
Systemic Lupus ◽  
Fibrinogen Molecule ◽  
Anticoagulant Property

There is paucity of information regarding the prolonged plasma thrombin time known to occur in some patients with systemic lupus erythematosus. Detailed investigations of plasma from two such patients disclosed that IgG accounted for this defect in each case. IgG isolated from plasma of either patient possessed the property of delaying fibrin aggregation and prolonging the clotting times of fibrinogen. Preincubation of IgG from either patient with anti-IgG or anti-Fab (rabbit) serum abolished this anticoagulant property. Moreover, the anticoagulant IgG from the first patient was neutralized with anit-k chain and anti-IgG3, that from the second patient with anti-lambda chain and anti-IgG1 serum. These anticoagulants were also dissimilar with respect to their interactions with fibrin(ogen). IgG from the first patient had no anticoagulant activity against fibrin(ogen) species lacking intact Aalpha chains. IgG from the second patient displayed undiminished anticoagulant effect on such fibrin(ogen) species. We conclude that each anticoagulant interacted with a distinct region(s) on the fibrinogen molecule and that these interactions affect or involve sites that participate in the fibrin self-assembly process.

scholarly journals Anticoagulant Activity of Sulfated Ulvan Isolated from the Green Macroalga Ulva rigida

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 291 ◽  
Author(s):  
Amandine Adrien ◽  
Antoine Bonnet ◽  
Delphine Dufour ◽  
Stanislas Baudouin ◽  
Thierry Maugard ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Activated Partial Thromboplastin Time ◽  
Coagulation Cascade ◽  
Sulfated Polysaccharides ◽  
Thrombin Time ◽  
Ulva Rigida ◽  
Sulfate Content ◽  
Red Algal ◽  
Dependent Pathway ◽  
Interesting Alternative

(1) Background: Brown and red algal sulfated polysaccharides have been widely described as anticoagulant agents. However, data on green algae, especially on the Ulva genus, are limited. This study aimed at isolating ulvan from the green macroalga Ulva rigida using an acid- and solvent-free procedure, and investigating the effect of sulfate content on the anticoagulant activity of this polysaccharide. (2) Methods: The obtained ulvan fraction was chemically sulfated, leading to a doubling of the polysaccharide sulfate content in a second ulvan fraction. The potential anticoagulant activity of both ulvan fractions was then assessed using different assays, targeting the intrinsic and/or common (activated partial thromboplastin time), extrinsic (prothrombin time), and common (thrombin time) pathways, and the specific antithrombin-dependent pathway (anti-Xa and anti-IIa), of the coagulation cascade. Furthermore, their anticoagulant properties were compared to those of commercial anticoagulants: heparin and Lovenox®. (3) Results: The anticoagulant activity of the chemically-sulfated ulvan fraction was stronger than that of Lovenox® against both the intrinsic and extrinsic coagulation pathways. (4) Conclusion: The chemically-sulfated ulvan fraction could be a very interesting alternative to heparins, with different targets and a high anticoagulant activity.

Heparin Chromatography

1981 ◽  
Author(s):  
R Losito ◽  
H Gattiker ◽  
G Bilodeau ◽  
B Longpré
Keyword(s):  
Gel Electrophoresis ◽  
Systematic Approach ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Weight Fraction ◽  
Activated Partial Thromboplastin Time ◽  
Thrombin Time ◽  
Agarose Gel Electrophoresis ◽  
Recalcification Time ◽  
Second Peak

Commercial heparin is not a homogeneous substance. The isolation of the active component still appears to be a problem and usually requires several different procedures. In order to see if it would be possible to simplify the purification, we studied and compared the chromatography of porcine heparin by a systematic approach using various types of adsorbants, gels and programmed elution techniques employing a Beckman Spectrochrom 130 chromatographic analyzer. A total of 20 combinations were attempted with 200 mg of heparin (169 u/mg) being chromatographed in each case. The fractions of each peak were tested by several methods and included the activated partial thromboplastin time, recalcification time, thrombin time, anti-factor Xa, chromogenic, USP assay, agarose gel electrophoresis and the measurement of metachromatic activity. It was found that dextran gel with an average dry diameter of 75-100 um produced the best chromatograms whose peaks were well resolved. The medium molecular weight fraction of the second peak contained half of the applied heparin. This peak contained all the anticoagulant activity besides reacting very strongly for metachromatic activity. The smaller and larger molecular fractions found in the other three peaks gave very weak metachromatic activity and were devoid of anticoagulant activity. These results have important implications in the preparation and clinical use of heparin.

scholarly journals Sulfated Polysaccharides Isolated from ClonedGrateloupia filicinaand Their Anticoagulant Activity

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Xiaolin Chen ◽  
Shengfeng Yang ◽  
Jinxia Wang ◽  
Lin Song ◽  
Ronge Xing ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Average Molecular Weight ◽  
Jiaozhou Bay ◽  
Activated Partial Thromboplastin Time ◽  
Sulfated Polysaccharides ◽  
Neutral Sugar ◽  
Thrombin Time ◽  
New Thought ◽  
Good Potential

Sulfated polysaccharides (GSP) were isolated from the clonedGrateloupia filicinawhich was cultured in Jiaozhou Bay, Qingdao, China. The yield of GSP was 15.75%. The total sugar and sulfate were 40.90 and 19.89%, respectively. And the average molecular weight was 11.7 KDa. The results of neutral sugar analysis showed that GSP was mainly sulfated polysaccharides of galactose. The experiments for activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) anticoagulant assays in vitro indicated that GSP was a good potential anticoagulant. Therefore, this study supplied new thought for the clonedGrateloupia filicinaexploitation of high-value products.

Copolymers of 2-acryloylamido-2-methylpropanesulfonic acid and acrylic acid with anticoagulant activity

e-Polymers ◽  
2003 ◽  
Vol 3 (1) ◽  
Author(s):  
Dilyana Paneva ◽  
Olya Stoilova ◽  
Nevena Manolova ◽  
Dobri Danchev ◽  
Zdravko Lazarov ◽  
...  
Keyword(s):  
Acrylic Acid ◽  
Prothrombin Time ◽  
Partial Thromboplastin Time ◽  
Anticoagulant Activity ◽  
Activated Partial Thromboplastin Time ◽  
Thrombin Time

Abstract Copolymers of 2-acryloylamido-2-methylpropanesulfonic acid (AMPS) and acrylic acid (AA), as well as the corresponding homopolymers PAMPS and PAA, were studied in vitro on human pool plasma for their anticoagulant activity. The values of the haemostatic parameters - prothrombin time, activated partial thromboplastin time and thrombin time - depend on the composition and the concentration of the (co)polymers. Reptilase time remains unchanged on adding the (co)polymers. A broad concentration range from 16 μg/ml to 3 mg/ml was studied. The copolymers possess anticoagulant activity, which is higher at higher content of AMPS units. It was found that, at certain concentrations, the haemostatic parameters of PAMPS are close to that of heparin. PAA has the lowest anticoagulant activity.

scholarly journals Monoclonal IgG anticoagulants delaying fibrin aggregation in two patients with systemic lupus erythematosus (SLE)

Blood ◽  
1978 ◽  
Vol 52 (5) ◽  
pp. 1037-1046 ◽  
Author(s):  
DK Galanakis ◽  
EM Ginzler ◽  
SM Fikrig
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Self Assembly ◽  
Anticoagulant Activity ◽  
Rabbit Serum ◽  
Distinct Region ◽  
Thrombin Time ◽  
Systemic Lupus ◽  
Fibrinogen Molecule ◽  
Anticoagulant Property

Abstract There is paucity of information regarding the prolonged plasma thrombin time known to occur in some patients with systemic lupus erythematosus. Detailed investigations of plasma from two such patients disclosed that IgG accounted for this defect in each case. IgG isolated from plasma of either patient possessed the property of delaying fibrin aggregation and prolonging the clotting times of fibrinogen. Preincubation of IgG from either patient with anti-IgG or anti-Fab (rabbit) serum abolished this anticoagulant property. Moreover, the anticoagulant IgG from the first patient was neutralized with anit-k chain and anti-IgG3, that from the second patient with anti-lambda chain and anti-IgG1 serum. These anticoagulants were also dissimilar with respect to their interactions with fibrin(ogen). IgG from the first patient had no anticoagulant activity against fibrin(ogen) species lacking intact Aalpha chains. IgG from the second patient displayed undiminished anticoagulant effect on such fibrin(ogen) species. We conclude that each anticoagulant interacted with a distinct region(s) on the fibrinogen molecule and that these interactions affect or involve sites that participate in the fibrin self-assembly process.

scholarly journals Structural Characteristics and Anticoagulant Property In Vitro and In Vivo of a Seaweed Sulfated Rhamnan

Marine Drugs ◽  
2018 ◽  
Vol 16 (7) ◽  
pp. 243 ◽  
Author(s):  
Xue Liu ◽  
Shuyao Wang ◽  
Sujian Cao ◽  
Xiaoxi He ◽  
Ling Qin ◽  
...  
Keyword(s):  
Degradation Products ◽  
Structural Characteristics ◽  
Anticoagulant Activity ◽  
Sulfated Polysaccharide ◽  
Thrombin Time ◽  
Drug Molecules ◽  
Monostroma Angicava ◽  
Anticoagulant Property

Great diversity and metabolite complexity of seaweeds offer a unique and exclusive source of renewable drug molecules. Polysaccharide from seaweed has potential as a promising candidate for marine drug development. In the present study, seaweed polysaccharide (SPm) was isolated from Monostroma angicava, the polymeric repeat units and anticoagulant property in vitro and in vivo of SPm were investigated. SPm was a sulfated polysaccharide which was mainly constituted by 3-linked, 2-linked-α-l-rhamnose residues with partially sulfate groups at C-2 of 3-linked α-l-rhamnose residues and C-3 of 2-linked α-l-rhamnose residues. Small amounts of xylose and glucuronic acid exist in the forms of β-d-Xylp(4SO4)-(1→ and β-d-GlcA-(1→. SPm effectively prolonged clotting time as evaluated by the activated partial thromboplastin time and thrombin time assays, and exhibited strong anticoagulant activity in vitro and in vivo. The fibrin(ogen)olytic and thrombolytic properties of SPm were evaluated by plasminogen activator inhibitior-1, fibrin degradation products, D-dimer and clot lytic rate assays using rats plasma, and the results showed that SPm possessed high fibrin(ogen)olytic and thrombolytic properties. These results suggested that SPm has potential as a novel anticoagulant agent.

Anticoagulant Activity of Hirulog™, a Direct Thrombin Inhibitor, in Humans

1993 ◽  
Vol 69 (02) ◽  
pp. 157-163 ◽  
Author(s):  
Irving Fox ◽  
Adrian Dawson ◽  
Peter Loynds ◽  
Jane Eisner ◽  
Kathleen Findlen ◽  
...  
Keyword(s):  
Rational Design ◽  
Therapeutic Potential ◽  
Anticoagulant Activity ◽  
Subcutaneous Administration ◽  
Direct Thrombin Inhibitor ◽  
Controlled Study ◽  
Thrombin Inhibitor ◽  
Thrombin Time ◽  
Thrombotic Disease ◽  
Dose Dependent

SummaryHirulog™ (BG8967) is a direct thrombin inhibitor built by rational design using the protein hirudin as a model (Maraganore et al. [1990]; Biochemistry 29: 7095–101). In order to evaluate the therapeutic potential for hirulog in the management of thrombotic disease, the tolerability and anticoagulant activity of the agent were examined in a study of human volunteers.In a randomized, placebo-controlled study (n = 54), the intravenous infusion of hirulog over 15 min showed a rapid, dose-dependent prolongation of activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). There was a corresponding dose-dependent increase in plasma hirulog levels. The peptide was rapidly cleared with a half-life of 36 min and a total body clearance rate for the peptide of 0.43 1 kg−1 h−1. Similar activity was observed following subcutaneous injection but with sustained pharmacodynamic and pharmacokinetic behavior. There was a significant correlation between pharmacokinetic and pharmacodynamic variables for both intravenous (r = 0.8, p <0.001) and subcutaneous administration (r = 0.7, p = 0.002).To evaluate the possible interactions of aspirin on the tolerability and anticoagulant activity of intravenous hirulog, a cross-over design was employed in eight subjects. Aspirin administration did not modify the peptide’s activity. At the administered dose of 0.6 mg kg−1 h−1 for 2 h, hirulog infusion prolonged APTT from 230 to 260% baseline. The infusion of hirulog in subjects who had received aspirin was not associated with any significant changes in the template bleeding time.The final phase of the study examined the activity and tolerability of hirulog in ten subjects during prolonged intravenous infusions for up to 24 h. The peptide (0.3 mg kg−1 h−1) exhibited sustained anticoagulant activity with no evidence for a cumulative effect. During hirulog infusion, APTT was prolonged from 210 to 250% baseline.In all phases of the study, hirulog administration was generally well-tolerated.Our observations show that hirulog is an active antithrombin agent with excellent tolerability in humans. As a direct thrombin inhibitor, hirulog provides a novel approach for the management of thrombotic disease.

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