Glycidyl methacrylate-crosslinked fish swim bladder as a novel cardiovascular biomaterial with improved antithrombotic and anticalcification properties

At present, commercial artificial biological valves are mostly prepared by crosslinking bovine or porcine pericardia with glutaraldehyde. Swim bladder has similar components and lower immunogenicity compared to bovine or porcine pericardium. In this study, we used a glycidyl methacrylate (GMA)–based radical polymerization method to crosslink decellularized swim bladders. Amino and carboxyl groups in the swim bladder were reacted with epoxy groups on GMA to introduce carbon–carbon double bonds to the swim bladder. The results showed that the platelet adhesion of GMA-crosslinked swim bladders (GMA-SBs) decreased by 35%, as compared to that of glutaraldehyde-crosslinked swim bladders (GLUT-SBs). Moreover, the superior anticoagulant property was further verified by the ex vivo arteriovenous shunt assay. Meanwhile, the subcutaneous implantation in rats showed that GMA-SBs were able to effectively inhibit the calcification compared with GLUT-SBs. In conclusion, GMA-SBs showed improved antithrombotic and anticalcification properties compared to GLUT-SBs.

Anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle aqueous extract

The current study explores the anticoagulant, fibrinogenolytic and anti-platelet aggregation activities of Lablab purpureus (L.) Sweet seed radicle extract (LPRE). It is firstly reported that LPRE has protein at a concentration of 1.5 mg/ml and further evaluated for protein in gel electrophoresis. The proteolytic activity of LPRE was evaluated using casein and gelatin as substrate. LPRE showed a specific activity of 0.35 U. LPRE increased the plasma clotting time significantly showing its anticoagulant property. LPRE hydrolyzed the A?, B? and ? chains of fibrinogen. Furthermore, LPRE significantly inhibited the platelet aggregation induced by agonist adenosine diphosphate (ADP).

Structural Characteristics and Anticoagulant Property In Vitro and In Vivo of a Seaweed Sulfated Rhamnan

Great diversity and metabolite complexity of seaweeds offer a unique and exclusive source of renewable drug molecules. Polysaccharide from seaweed has potential as a promising candidate for marine drug development. In the present study, seaweed polysaccharide (SPm) was isolated from Monostroma angicava, the polymeric repeat units and anticoagulant property in vitro and in vivo of SPm were investigated. SPm was a sulfated polysaccharide which was mainly constituted by 3-linked, 2-linked-α-l-rhamnose residues with partially sulfate groups at C-2 of 3-linked α-l-rhamnose residues and C-3 of 2-linked α-l-rhamnose residues. Small amounts of xylose and glucuronic acid exist in the forms of β-d-Xylp(4SO4)-(1→ and β-d-GlcA-(1→. SPm effectively prolonged clotting time as evaluated by the activated partial thromboplastin time and thrombin time assays, and exhibited strong anticoagulant activity in vitro and in vivo. The fibrin(ogen)olytic and thrombolytic properties of SPm were evaluated by plasminogen activator inhibitior-1, fibrin degradation products, D-dimer and clot lytic rate assays using rats plasma, and the results showed that SPm possessed high fibrin(ogen)olytic and thrombolytic properties. These results suggested that SPm has potential as a novel anticoagulant agent.

Anticoagulant Effects of Glycosaminoglycan Extracted from Fish Scales

Background: Fisheries wastes are the unique sources of natural products which structural and chemical characteristics of their extracted compounds are different from those of terrestrial animals. They are known as a rich source of bioactive molecules, including collagen and glycosaminoglycan (GAGs).In the present study, we extracted and analysed anticoagulant activity of glycosaminoglycan from fish (Rutilus frisii kuum) scales Materials and Methods: The glycosaminoglycan compounds were extracted using cationic salt of cetylpyridinium chloride (CPC) and Fourier transform infrared spectroscopy (FTIR) spectrum was used to identify and compare the structure of the extracted glycosaminoglycan with heparin. Anticoagulant property of extracted glycosaminoglycan was measured by prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT) on human blood at three concentrations of 50, 100, and 200 µg/ml. Results: The value of the extracted glycosaminoglycan was estimated approximately 23.8 mg/g of dry tissue.FTIR analysis results confirmed the presence of heparin-like compounds in fish scales glycosaminoglycan. Human Plasma coagulation time increased significantly with extracted GAGs concentration incriminated. Since at a concentration of 200 µg/ml, coagulation time as aPTT was 4.3 times that of control and coagulation time was prolonged about 138.6 seconds. Conclusions: The results of the current study showed that the glycosaminoglycan extracted from fish scales had valuable anticoagulant property compared to synthetic anticoagulant compounds such as heparin.

Platelet Adhesion on the Silk Fibroin Material Surface

Silk fibroin (SF) has good biocompatibility and has been used to construct small caliber artificial blood vessels. As a vascular graft, the antithrombogenicity is the first important performance. The aim of this study was to evaluate the anticoagulant property of polyethylene glycol diglycidyl ether (PEG-DE) crosslinked and ethanol treated SF materials by investigating the platelet adhesion. Dacron and PTFE were parallel studied as the controls. Results showed that: the platelet adhesion ratios on the ethanol treated SF films were significantly lower than that on the Dacron and PTFE; the platelet adhesion ratios on the PEG-DE crosslinked SF films were higher than that on the ethanol treated SF films and slightly increased with increasing the proportion of the PEG-DE. But the platelet adhesion ratios on all of the PEG-DE crosslinked SF films were significantly lower than that on the controls.