scholarly journals Olfactory receptor neurons from antennae of developing male Manduca sexta respond to components of the species-specific sex pheromone in vitro

1992 ◽  
Vol 12 (7) ◽  
pp. 2523-2531 ◽  
Author(s):  
M Stengl ◽  
F Zufall ◽  
H Hatt ◽  
JG Hildebrand
Keyword(s):  
Sex Pheromone ◽  
Manduca Sexta ◽  
Olfactory Receptor ◽  
Olfactory Receptor Neurons ◽  
Receptor Neurons ◽  
Species Specific

scholarly journals Ionic currents of cultured olfactory receptor neurons from antennae of male Manduca sexta

1991 ◽  
Vol 11 (4) ◽  
pp. 956-965 ◽  
Author(s):  
F Zufall ◽  
M Stengl ◽  
C Franke ◽  
JG Hildebrand ◽  
H Hatt
Keyword(s):  
Manduca Sexta ◽  
Olfactory Receptor ◽  
Ionic Currents ◽  
Olfactory Receptor Neurons ◽  
Receptor Neurons

scholarly journals Inhibitory Odorant Signaling in Mammalian Olfactory Receptor Neurons

2010 ◽  
Vol 103 (2) ◽  
pp. 1114-1122 ◽  
Author(s):  
Kirill Ukhanov ◽  
Elizabeth A. Corey ◽  
Daniela Brunert ◽  
Katharina Klasen ◽  
Barry W. Ache
Keyword(s):  
Olfactory Receptor ◽  
Olfactory Receptor Neurons ◽  
Electrophysiological Response ◽  
Cellular Processes ◽  
Receptor Neurons ◽  
Phosphoinositide 3 Kinase ◽  
Mixture Suppression ◽  
G Protein Coupled ◽  
Kinetics Of

Odorants inhibit as well as excite olfactory receptor neurons (ORNs) in many species of animals. Cyclic nucleotide-dependent activation of canonical mammalian ORNs is well established but it is still unclear how odorants inhibit these cells. Here we further implicate phosphoinositide-3-kinase (PI3K), an indispensable element of PI signaling in many cellular processes, in olfactory transduction in rodent ORNs. We show that odorants rapidly and transiently activate PI3K in the olfactory cilia and in the olfactory epithelium in vitro. We implicate known G-protein–coupled isoforms of PI3K and show that they modulate not only the magnitude but also the onset kinetics of the electrophysiological response of ORNs to complex odorants. Finally, we show that the ability of a single odorant to inhibit another can be PI3K dependent. Our collective results provide compelling support for the idea that PI3K-dependent signaling mediates inhibitory odorant input to mammalian ORNs and at least in part contributes to the mixture suppression typically seen in the response of ORNs to complex natural odorants.

scholarly journals Odor sensitivity of cultured lobster olfactory receptor neurons is not dependent on process formation.

1993 ◽  
Vol 174 (1) ◽  
pp. 215-233 ◽  
Author(s):  
D A Fadool ◽  
W C Michel ◽  
B W Ache
Keyword(s):  
Voltage Clamp ◽  
Olfactory Receptor ◽  
Cell Voltage ◽  
Response Spectra ◽  
Olfactory Receptor Neurons ◽  
Process Formation ◽  
Receptor Neurons ◽  
Dual Polarity ◽  
Odor Sensitivity

Cultured lobster olfactory receptor neurons (ORNs) were surveyed for their odor sensitivity with whole-cell, voltage-clamp recording. The nature of the adequate stimuli, the degree of tuning (response spectra) of the cells, the threshold of sensitivity and the dual polarity of the odor-evoked currents are consistent with chemosensitivity in the cultured ORNs being olfactory. The ability of odors to evoke currents in cultured ORNs that lack processes suggests that lobster ORNs can be induced in vitro to insert all the elements of the transduction cascade in the soma, including those that might normally be confined to processes. This should greatly facilitate analysis of olfactory transduction in these cells.

Cyclic Nucleotide-Activated Currents in Cultured Olfactory Receptor Neurons of the Hawkmoth Manduca sexta

2008 ◽  
Vol 100 (5) ◽  
pp. 2866-2877 ◽  
Author(s):  
Steffi Krannich ◽  
Monika Stengl
Keyword(s):  
Manduca Sexta ◽  
Cyclic Nucleotide ◽  
Olfactory Receptor ◽  
Low Voltage ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Olfactory Receptor Neurons ◽  
Cation Channels ◽  
Receptor Neurons

Moth pheromones cause rises in intracellular Ca2+ concentrations that activate Ca2+-dependent cation channels in antennal olfactory receptor neurons. In addition, mechanisms of adaptation and sensitization depend on changes in cyclic nucleotide concentrations. Here, cyclic nucleotide-activated currents in cultured olfactory receptor neurons of the moth Manduca sexta are described, which share properties with currents through vertebrate cyclic nucleotide-gated channels. The cyclic nucleotide-activated currents of M. sexta carried Ca2+ and monovalent cations. They were directly activated by cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), modulated by Ca2+/calmodulin, and inhibited by lanthanum. M. sexta cyclic nucleotide-activated currents developed in an all-or-none manner, which suggests that the underlying channels are coupled and act coordinately. At least one cAMP- and two cGMP-activated nonselective cation currents could be distinguished. Compared with the cAMP-activated current, both cGMP-activated currents appeared to conduct more Ca2+ and showed a stronger down-regulation by Ca2+/calmodulin-dependent negative feedback. Furthermore, both cGMP-activated currents differed in their Ca2+-dependent inhibition. Thus M. sexta olfactory receptor neurons, like vertebrate sensory neurons, appear to express nonselective cyclic nucleotide-activated cation channels with different subunit compositions. Besides the nonselective cyclic nucleotide-activated cation currents, olfactory receptor neurons express a cAMP-dependent current. This current resembled a protein kinase-modulated low-voltage–activated Ca2+ current.

scholarly journals Comparison of the Nasal Olfactory Organs of Various Species of Lizardfishes (Teleostei: Aulopiformes: Synodontidae) with Additional Remarks on the Brain

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
L. Fishelson ◽  
D. Golani ◽  
B. Galil ◽  
M. Goren
Keyword(s):  
Olfactory Epithelium ◽  
Olfactory Receptor ◽  
Olfactory Receptor Neurons ◽  
Adult Fish ◽  
Supporting Cells ◽  
Receptor Neurons ◽  
Olfactory Organs ◽  
Species Specific ◽  
The Brain

The olfactory organs of lizardfishes (Synodontidae) are situated in two capsules connected to the outside by incurrent and excurrent openings. The olfactory epithelium is in form of petal rosettes each composed of lamellae and a rephe, and bear olfactory receptor neurons, supporting cells and cells with kinocillia. The dimension of rosettes and lamellae, as well as the number of lamellae, increase with growth of the fish; until in adult fish these parameters remaine constant, species specific. In adultSynodusspp. andTrachinocephalus myopsthe rosettes are 3.5–4.0 mm long, with 5–8 lamellae, whereas inSauridaspp. they are 8.0 mm and possess up tp 22 lamellae. The number of ORN ranges from 2,600 on the smaller lamellae to 20,000 on the largest ones. The number of ORN/m of olfactory is ca. 30,000 inSauridaspp. Thus the rosettes ofS. macrolepiswith 20 lamellae possess a total of ca. 170,000 ORN, whereas those ofSy. variegatusandT. myopswith the average of six lamellae possess only ca. 50,000–65,000 ORN. The olfactory nerves lead from the rosettes to the olfactory balbs situated on the olfactory lobes. The differences among the species in olfactory organs are discussed in correlation with their distribution.

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