scholarly journals The Preparation of Reversed Micelle Containing Water Soluble Collagen Solution and Their Application on Lip Make up Products

2013 ◽  
Vol 39 (4) ◽  
pp. 271-279
Author(s):  
Young-Ho Kim ◽  
Eun-Ji Jung ◽  
Dong-Won Lee ◽  
Sang-Gil Lee ◽  
Hyeong-Bae Pyo
Biomaterials ◽  
2000 ◽  
Vol 21 (4) ◽  
pp. 415-419 ◽  
Author(s):  
Takashi Nezu ◽  
Françoise M Winnik

2020 ◽  
Author(s):  
Andrey Baryshnikov ◽  
Svetlana Derkach ◽  
Nadezhda Shumskaya ◽  
Kira Rysakova ◽  
Ivan Lyzhov ◽  
...  

The results of the isolation of collagen hydrolysates from tissues of two Arctic marine organisms are presented. Extraction and use of marine organism collagen is a part of complex waste less processing of sea catches. Possible ways of preparing water-soluble collagen hydrolysates from different sources were studied. It is established that for preparing soluble collagen hydrolysate from skin of cod Gadus morhua acid hydrolysis in 0.3% acetic acid is suitable. Purification of solution by means of ultrafiltration gives a pure collagen hydrolysate with mass fraction of the main substance about 98%. Collagen of muscular skin bag of holothuria Molpadia borealis is almost insoluble in acid and alkali solutions. The major mass of collagen remains in an insoluble residue. The analysis of infrared spectrum transmission and chemical analysis of the general carbohydrates and collagen in different fractions showed that as a result of acid and alkaline processing of raw materials the glycosaminoglycans (GAG) and some quantity of collagen are extracted, their main quantity was determined in alkaline extracts. For extraction of soluble collagen from insoluble residue it is required enzymatic by pepsin in acidic medium. Properties of holothurian collagen and fish collagen are different. The preparation of water-soluble collagen derivatives requires using enzymatic hydrolysis.


2016 ◽  
Vol 137 ◽  
pp. 410-417 ◽  
Author(s):  
Min Zhang ◽  
Cuicui Ding ◽  
Junhui Yang ◽  
Shan Lin ◽  
Lihui Chen ◽  
...  

2018 ◽  
Vol 18 (1) ◽  
pp. 251-260
Author(s):  
Beata Głowińska ◽  
Krzysztof Młynek ◽  
Alicja Dzido ◽  
Ewa Salomończyk

Abstract Most scientific studies are dedicated to the possibility of preparing beef for consumption under industrial conditions. Few publications are devoted to the issue of collagen thermohydrolysis in conditions available to the consumer. This study has analyzed the effect of small values of water vapor pressure on major culinary indices and chemical components of the longissimus lumborum muscle obtained from bulls with different growth rates. The experiment involved 48 animals. On the basis of the gain during the fattening time, the animals were divided into a low growth intensity group, with a daily body weight gain of ≤900 g, and a high growth intensity group with a daily gain of >900 g/day. A part of the samples of the longissimus lumborum muscle (control) was thermally treated in a water bath at 75°C. Another part was heat treated in a pressure-pot at 150°C, at a pressure of 0.1 MPa. The next part of samples was subjected to the same temperature, but the pressure was 0.2 MPa. The obtained results indicate that the values of the studied indices were largely affected by thermal processing parameters rather than the animals’ growth rate. The highest contents of total protein and water-soluble collagen were obtained in the case of a temperature of 150°C and the highest pressure (0.2 MPa). Water vapor with increased temperature and pressure also created favorable conditions for obtaining better meat tenderness and more favorable values of the water holding capacity. The latter characteristic appeared to be strongly connected with an increasing amount of water-soluble collagen, which was confirmed by relatively high values of the correlation coefficient between these characteristics. A strong positive correlation was also shown between thermal drip and the total collagen content in meat.


Author(s):  
J. G. Robertson ◽  
D. F. Parsons

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.


Author(s):  
J. D. McLean ◽  
S. J. Singer

The successful application of ferritin labeled antibodies (F-A) to ultrathin sections of biological material has been hampered by two main difficulties. Firstly the normally used procedures for the preparation of material for thin sectioning often result in a loss of antigenicity. Secondly the polymers employed for embedding may non-specifically absorb the F-A. Our earlier use of cross-linked polyampholytes as embedding media partially overcame these problems. However the water-soluble monomers used for this method still extract many lipids from the material.


Author(s):  
D.R. Mattie ◽  
J.W. Fisher

Jet fuels such as JP-4 can be introduced into the environment and come in contact with aquatic biota in several ways. Studies in this laboratory have demonstrated JP-4 toxicity to fish. Benzene is the major constituent of the water soluble fraction of JP-4. The normal surface morphology of bluegill olfactory lamellae was examined in conjunction with electrophysiology experiments. There was no information regarding the ultrastructural and physiological responses of the olfactory epithelium of bluegills to acute benzene exposure.The purpose of this investigation was to determine the effects of benzene on the surface morphology of the nasal rosettes of the bluegill sunfish (Lepomis macrochirus). Bluegills were exposed to a sublethal concentration of 7.7±0.2ppm (+S.E.M.) benzene for five, ten or fourteen days. Nasal rosettes were fixed in 2.5% glutaraldehyde and 2.0% paraformaldehyde in 0.1M cacodylate buffer (pH 7.4) containing 1.25mM calcium chloride. Specimens were processed for scanning electron microscopy.


Author(s):  
H. J. Arnott ◽  
M. A. Webb ◽  
L. E. Lopez

Many papers have been published on the structure of calcium oxalate crystals in plants, however, few deal with the early development of crystals. Large numbers of idioblastic calcium oxalate crystal cells are found in the leaves of Vitis mustangensis, V. labrusca and V. vulpina. A crystal idioblast, or raphide cell, will produce 150-300 needle-like calcium oxalate crystals within a central vacuole. Each raphide crystal is autonomous, having been produced in a separate membrane-defined crystal chamber; the idioblast''s crystal complement is collectively embedded in a water soluble glycoprotein matrix which fills the vacuole. The crystals are twins, each having a pointed and a bidentate end (Fig 1); when mature they are about 0.5-1.2 μn in diameter and 30-70 μm in length. Crystal bundles, i.e., crystals and their matrix, can be isolated from leaves using 100% ETOH. If the bundles are treated with H2O the matrix surrounding the crystals rapidly disperses.


Author(s):  
B. J. Grenon ◽  
A. J. Tousimis

Ever since the introduction of glutaraldehyde as a fixative in electron microscopy of biological specimens, the identification of impurities and consequently their effects on biologic ultrastructure have been under investigation. Several reports postulate that the impurities of glutaraldehyde, used as a fixative, are glutaric acid, glutaraldehyde polymer, acrolein and glutaraldoxime.Analysis of commercially available biological or technical grade glutaraldehyde revealed two major impurity components, none of which has been reported. The first compound is a colorless, water-soluble liquid with a boiling point of 42°C at 16 mm. Utilizing Nuclear Magnetic Resonance (NMR) spectroscopic analysis, this compound has been identified to be — dihydro-2-ethoxy 2H-pyran. This impurity component of the glutaraldehyde biological or technical grades has an UV absorption peak at 235nm. The second compound is a white amorphous solid which is insoluble in water and has a melting point of 80-82°C. Initial chemical analysis indicates that this compound is an aldol condensation product(s) of glutaraldehyde.


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