STUDIES ON THE EFFECT OF DIETHYLSTILBESTROL ON SERUM CHOLESTEROL AND INORGANIC PHOSPHORUS CONCENTRATIONS IN DIFFERENT GROUPS OF EXPERIMENTAL ANIMALS

<p>Hypercholesterolemia is a risk factor for atherosclerosis and vascular diseases. Medicinal plants with different content of secondary metabolites such as polyphenols and flavonoids are natural sources to cope hypercholesterolemia. This study aimed to determine the effect of ethyl acetate fraction of piladang leaves (<em>Solenostemon scutellarioides</em> (L) Codd) on serum cholesterol levels in hypercholesterolemic male rats. Rats were divided into 6 groups, each consisting of 6 animals: group I (negative control), group II (positive control), group III, IV and V were each given fraction of ethyl acetate doses respectively 100, 250 and 500 mg/kg and group VI (simvastatin as a comparator). Measurement of serum cholesterol levels were conducted with CHOD-PAP method using a photometer 5010. Result showed ethyl acetate fraction affects cholesterol levels of experimental animals with a lower value than the positive control. According to statistics of one way ANOVA followed by the Duncan test method SPSS 17 showed no significant differences (p> 0.05) on cholesterol levels in all given doses of ethyl acetate fraction. Group III(a dose of 100 mg / kg) was probably the most effective in lowering the blood cholesterol levels of experimental animals.</p>

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CHANGES IN HUMAN SERUM PROTEIN FRACTIONS WITH AGE AND WEIGHT

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o61-057 ◽

1961 ◽

Vol 39 (3) ◽

pp. 569-579 ◽

Cited By ~ 10

Author(s):

B. C. Das ◽

S. K. Bhattacharya

Keyword(s):

Serum Protein ◽

Serum Cholesterol ◽

Serum Proteins ◽

Inorganic Phosphorus ◽

Total Serum ◽

Total Serum Cholesterol ◽

Adult Males ◽

Serum Globulin ◽

Human Serum Protein ◽

Protein Serum

Serum protein, serum cholesterol, and inorganic phosphorus measurements were determined for 160 adult males. Height, weight, and age data were also collected. The serum proteins were separated into six components in terms of their electrophoretic mobility: albumin, α1 globulin, α2 globulin, β1 globulin, β2 globulin, and γ globulin. Analysis of the results showed that serum globulin fractions and total serum globulin increase linearly with age (P <.01), and decrease as weight increases (P <.05). Total serum cholesterol, cholesterol ester, and free cholesterol were not related to serum globulin fractions. Inorganic phosphorus was negatively related to the globulin fractions (P <.05). Serum albumin did not change with age, weight, height, cholesterol, or inorganic phosphorus. Albumin and globulin fractions were significantly related to each other (P <.01).

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Association between serum cholesterol, brain serotonin, and anxiety: A study in simvastatin administered experimental animals

International journal of Nutrition Pharmacology Neurological Diseases ◽

10.4103/2231-0738.124617 ◽

2014 ◽

Vol 4 (1) ◽

pp. 69 ◽

Cited By ~ 2

Author(s):

JayaMary Thomas ◽

Joyamma Varkey ◽

BibinBaby Augustine

Keyword(s):

Serum Cholesterol ◽

Brain Serotonin ◽

Experimental Animals

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BIOMETRY OF CALCIUM, INORGANIC PHOSPHORUS, CHOLESTEROL, AND LIPOID PHOSPHORUS IN THE BLOOD OF RABBITS

Journal of Experimental Medicine ◽

10.1084/jem.48.4.549 ◽

1928 ◽

Vol 48 (4) ◽

pp. 549-565 ◽

Cited By ~ 9

Author(s):

Alvin R. Harnes

Keyword(s):

Blood Serum ◽

Serum Cholesterol ◽

Whole Blood ◽

Inorganic Phosphorus ◽

Mathematical Significance ◽

Coefficient Of Correlation ◽

Blood Constituents ◽

Mathematical Correlation

Determinations of calcium, inorganic phosphorus, cholesterol and lipoid phosphorus were made on a series of animals recently received from the dealer for the purpose of determining the trends of these 4 blood constituents throughout the year with the degree of their respective variations and mathematical correlation. It was found that for the 80 animals examined, calcium varied from 14.5 ±0.10 to 18.5 ±0.39 mg. and inorganic phosphorus 4.960 ±0.20 to 6.820 ±0.20 mg. per 100 cc. of blood serum. Cholesterol varied from 51.1 ±1.18 to 73.3 ±1.34 mg. and lecithin from 94.8 ±1.397 to 168.3 ±10.18 mg. per 100 cc. of whole blood. Of the 6 possible combinations in calculating the coefficient of correlation for the trend throughout the experiment, 3 stand out as of mathematical significance, namely the following. Between inorganic phosphorus and lecithin the coefficient of correlation was found to be –0.794 ±0.088; between calcium and cholesterol –0.887 ±0.051 and between cholesterol and lecithin +0.560 ±0.164.

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Analytical Electron Microscopy (AEM) of Meningeal Cells Exposed to Particulate Cobalt During Studies of Experimental Epilepsy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053590 ◽

1982 ◽

Vol 40 ◽

pp. 186-187

Author(s):

R.G. Frederickson ◽

R.G. Ulrich ◽

J.L. Culberson

Keyword(s):

Electron Microscopy ◽

Analytical Electron Microscopy ◽

Experimental Epilepsy ◽

Metallic Cobalt ◽

Experimental Animals ◽

X Ray ◽

Brain Surface ◽

Meningeal Cells ◽

Analytical Electron ◽

The Brain

Metallic cobalt acts as an epileptogenic agent when placed on the brain surface of some experimental animals. The mechanism by which this substance produces abnormal neuronal discharge is unknown. One potentially useful approach to this problem is to study the cellular and extracellular distribution of elemental cobalt in the meninges and adjacent cerebral cortex. Since it is possible to demonstrate the morphological localization and distribution of heavy metals, such as cobalt, by correlative x-ray analysis and electron microscopy (i.e., by AEM), we are using AEM to locate and identify elemental cobalt in phagocytic meningeal cells of young 80-day postnatal opossums following a subdural injection of cobalt particles.

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Ultrastructure and analytical microprobe analysis of simian lung mite pigments

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143249 ◽

1986 ◽

Vol 44 ◽

pp. 324-325

Author(s):

R. W. Cole ◽

J. C. Kim

Keyword(s):

Biomedical Research ◽

Tissue Damage ◽

Microprobe Analysis ◽

Old World Monkeys ◽

Old World ◽

Experimental Animals ◽

The Past ◽

Lung Mite ◽

Mite Infestations ◽

Cardiopulmonary System

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.

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