PENGARUH FRAKSI ETIL ASETAT DAUN PILADANG (Solenostemon scutellarioides (L) Codd) TERHADAP KADAR KOLESTEROL TOTAL TIKUS PUTIH JANTAN

2017 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Verawati Verawati ◽  
Mimi Aria ◽  
Iyun Julia Ningsih
Keyword(s):  
Ethyl Acetate ◽  
Serum Cholesterol ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Control Group ◽  
Experimental Animals ◽  
Group Iii ◽  
Cholesterol Levels ◽  
Solenostemon Scutellarioides ◽  
Group I

<p>Hypercholesterolemia is a risk factor for atherosclerosis and vascular diseases. Medicinal plants with different content of secondary metabolites such as polyphenols and flavonoids are natural sources to cope hypercholesterolemia. This study aimed to determine the effect of ethyl acetate fraction of piladang leaves (<em>Solenostemon scutellarioides</em> (L) Codd) on serum cholesterol levels in hypercholesterolemic male rats. Rats were divided into 6 groups, each consisting of 6 animals: group I (negative control), group II (positive control), group III, IV and V were each given fraction of ethyl acetate doses respectively 100, 250 and 500 mg/kg and group VI (simvastatin as a comparator). Measurement of serum cholesterol levels were conducted with CHOD-PAP method using a photometer 5010. Result showed ethyl acetate fraction affects cholesterol levels of experimental animals with a lower value than the positive control. According to statistics of one way ANOVA followed by the Duncan test method SPSS 17 showed no significant differences (p&gt; 0.05) on cholesterol levels in all given doses of ethyl acetate fraction. Group III(a dose of 100 mg / kg) was probably the most effective in lowering the blood cholesterol levels of experimental animals.</p>

scholarly journals AKTIVITAS FRAKSI ETIL ASETAT KULIT BUAH JERUK NIPIS (Citrus aurantifolia (Christ) Swing) SEBAGAI ANTIPIRETIK PADA MENCIT YANG DIINDUKSI VAKSIN DPT

2021 ◽  
Vol 6 (2) ◽  
pp. 341-349
Author(s):  
Satrio Wibowo Rahmatullah ◽  
◽  
Eka Fitri Susiani ◽  
Muhammad Reza Fahlevi ◽  
Guntur Kurniawan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Group Treatment ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Citrus Aurantifolia ◽  
Antipyretic Activity ◽  
Group I ◽  
Test Result

Lime (Citrus aurantifolia (Christm.) Swing) is one of the types of oranges containing elements of compounds that are beneficial, so widely used as traditional medicine. This research aims to know the antipyretic activity of ethyl acetate fraction peel of lime. Phytochemical screening test result fraction ethyl acetate lemon rind contains flavonoids, saponins, alkaloids and essential oils. Total of 25 mice were divided into 5 groups, group I negative control, group II positive control, group III,IV,V were given ethyl acetate fraction at a dose of 0,07%/gBW,, 0,105%/gBWand 0,14%/gBW. Based on the results of graph average temperatures decrease the dose that the third has antipyretic activity because it can lower the temperature and the largest decreased in temperature at a dose 0,14%/gBW. Based on the test result ANOVA obtained sig. value 0,000. which means there is a temperature difference of meaning between 5 group treatment. And on Independent Test Sample T-test between a positive control with a dose 0,14%/gBW the value of sig. 0,601 (> 0,05) which means there is no meaningful difference.

Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

2020 ◽  
Author(s):  
Hendrika Andriana Silitonga ◽  
◽  
Gontar Alamsyah Siregar ◽  
Rosita Juwita Sembiring ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Interleukin 8 ◽  
Control Group ◽  
Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

UJI POTENSI EKSTRAK DAUN BENALU BATU (BEGONIA SP) TERHADAP KADAR GLUKOSA DALAM DARAH DAN GAMBARAN HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (RATTUS NORVEGICUS)

2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani
Keyword(s):  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Glucose Levels ◽  
White Rats ◽  
Group I ◽  
Ethanol Extracts

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.

scholarly journals ISONIAZID (INH)

2018 ◽  
Vol 25 (10) ◽  
pp. 1587-1595
Author(s):  
Umer Aleem ◽  
Rahman Shah ◽  
Noor Khan ◽  
M. Suliman
Keyword(s):  
Serum Bilirubin ◽  
Statistical Significance ◽  
Negative Control Group ◽  
Protective Role ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group 2

Objectives: Hepatotoxicity is the most complicated side effect of isoniazid (inh)in the patient treated for tuberculosis. In causes 8–30% hepatotoxicity in the developing world.Metabolism of INH produces a metabolite, called acetyl isoniazid. In this study hepatoprotectiveeffect of honey, in isoniazid induced animal model was assessed. Study Design: Randomizedcontrol trial. Setting: Saidu Medical College, Saidu Sharif Swat, KP. Period: October ToDecember 2017. Material and Methods: 40 healthy male rabbits were assigned randomly tothe group i, ii, iii and iv by using lottery method. Ten animals were grouped each row. Theisoniazid-induced hepatotoxic model was created by giving 50 mg inh/kg orally on daily basisfor eleven days. Group i was taken as negative control group ii as a positive control. Group iii andiv were experimental groups treated with 50 mg /kg/day and 100 mg /kg/day buckwheat honeyrespectively for eleven days. SPSS Version 16 software was used, mean, s.d. were determinedin all the groups. Values of serum bilirubin, sgpt, and alkaline phosphatase were comparedwith each other using pairt-test. Results: SGPT, Serum bilirubin, and alkaline phosphatasewere obtained in all the animals. Comparing group 1 negative control with group 2, 3 and 4shows statistical significance, (p=0.00). Comparing group 2 positive control with 3 and 4 showsstatistical significance, (p=0.00). Further comparing group 3 with group 4 also shows statisticalsignificance (p=0.00). Conclusion: From the above finding, it has been revealed that honeyhas got a protective effect in regressing hepatitis that has been induced in rabbit’s model byhigh doses of isoniazid. Related studies performed in which different chemicals and drugs havebeen tried for their protective role in isoniazid induced hepatitis also shows a similar type ofresults.

scholarly journals The test of improvement activity on kidney function lansau etanol extract by Muna regency on male wistra white rat

2020 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
Ruslin R
Keyword(s):  
Kidney Function ◽  
Dose Group ◽  
Ethanol Extract ◽  
Positive Control ◽  
Control Group ◽  
Negative Group ◽  
Local Society ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Background: Lansau is one of the traditional ingredients that consist of 44 types of plants that are believed by the local society as a nutritious herb to treat various diseases that exist in the society of Muna Southeast Sulawesi Province that has been used for hundreds of years for generations. Methods: The study was conducted to determine the effect of ethanol extract of Lansau on the improvement activity of kidney function that induced gentamicin-piroxicam. A total of 24 rats were used in which 20 ratswere modeled with damage of renal function using gentamicin-piroxicam drug which was then grouped into 5 groups. The first group was treated using a Lansau extract with doseI , the second group was treated with aLansauextract dose II, the third group was treated with the Lansau extract dose III, the fourth group was treated with ketosteryl, the fifth group was no treatment only given Na. 1% CMC and the sixth group is the normal group. The Modeling was performed for 7 days and on the eighth day blood sampling was taken to measure creatinine and urea levels. As for therapy performed for 4 weeks and every end of the week also made blood taking for measurement of creatinine and urea. Results: The highest percentage of creatinin decrease for each group was negative group -8.92%,positive group control was 84.21%,dose group I 74.75%, dose group II 80.07%, and dose group III 84.08%. For the highest percentage of decrease of ureum level for each group that is negative group -31.l49%,positive control group 49.59%,dose group I 40.53%, dose group II 26.79%, and dose group III 43.32%. Conclusion: The results showed that Lansau ethanol extract has the potential and effectiveness in the repair of kidney function damage.

scholarly journals Immunomodulator Activity of Effervescent Granule of Wualae Fruit (Etlingera elatior (Jack) R.M. Smith) Based on Specific Phagocytic Activity

2019 ◽  
Vol 2 (2) ◽  
pp. 35-40 ◽  
Author(s):  
Adryan Fristiohady ◽  
Wa Ode Siti Zubaydah ◽  
Wahyuni Wahyuni ◽  
Mirda Mirda ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Etlingera Elatior

The previous study reported that Etlingera elatior (Wualae) has activity as immunostimulant with dose at 300 mg/kg BW and 400 mg/kg BW. Formulating natural product into effervescent granule (GE) can increase practicality and interest to consume traditional medicine. This study aims to investigate the immunostimulant activity of Wualae ethanolic extract in the effervescent granule. Wualae was macerated with ethanol then formulated into effervescent granule at dose 300 mg/kgBW and 400 mg/kgBW. Formulations were evaluated. Animals were divided into 4 groups and treated with: group I (positive control); group II (negative control); group III (GE dose at 300mg/kgBW); and group IV (GE at dose 400mg/kgBW) for 7 days and at days 8, groups were infected by Staphylococcus aureus intraperitoneally. Immunostimulant activity was measured by calculating the Specific Phagocytic Activity (SPA) of macrophage. Data collected from the evaluation and measurement of the SPA then analyzed using SPSS. Results from the evaluation were considered good except the moisture content of effervescent granule. Results from SPA was found that effervescent granule at dose 300 mg/kgBW and at dose 400 mg/kgBW have 26.33% and 32.5% consecutively with significance 0.02 (p<0.05). Thus, effervescent granule has comparable phagocytosis activity with positive control.

scholarly journals UJI PENURUNAN KADAR GLUKOSA DARAH OLEH EKSTRAK ETANOL 70% DAUN BUNCIS (Phaseolus vulgaris L) PADA KELINCI JANTAN YANG DIBEBANI GLUKOSA

Biomedika ◽  
2012 ◽  
Vol 5 (1) ◽  
Author(s):  
Desy Kurniawati ◽  
EM Sutrisna ◽  
Arifah Sri Wahyuni
Keyword(s):  
Blood Glucose ◽  
Phaseolus Vulgaris ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Phaseolus Vulgaris L ◽  
Group Iii ◽  
Trust Level ◽  
Group I

This research aim to know the descent of blood glucose rate by extract etanol 70% of bean leaf at male rabbit which induced glucose. Research using the method test glucose tolerance of oral was including category research of sham eksperimental with complete random attempt device of unidirectional pattern. This research use 20 male rabbits which divided in 5 treatment group. Group I given by CMC Na 1% as negative control, group II given by acarbose suspensi 2,33 mg/kgBB as positive control, group III, IV and V given by extract etanol 70% bean leaf with each dose 200, 300 and 450 mg/kgBB. Treatment given at once after test animal induced glucose 50% ( 5 ml/1,5 kgBB). Glucose blood rate to define every 30 minute, 0 minute until 300 minutes by using method of enzimatik GOD PAP (Glucose Oxidase Phenol Aminoantipyrina Peroxidase), what was later then analysed with one track Anava and continued by test of LSD (Least Significant Diffeerence) with trust level 95%. Extract etanol 70% bean leaf with dose 200, 300 and 450 mg/kgBB have effect descent of blood glucose rate to negative control of CMC Na 1% (p = 0,000) with each percentage descent of blood glucose rate (% PKGD) 80,88 ± 0,93 ; 82,73 ± 0,67 and 85,32 ± 0,21.Keyword : Extract etanol 70%, bean leaf (Phaseolus vulgaris L), degradation of glucose rate

scholarly journals Analgesic Activity of Ethanolic Extracts of Karika Leaves (Carica pubescens) In Vivo

2016 ◽  
Vol 1 (2) ◽  
pp. 83 ◽  
Author(s):  
Heru Sasongko ◽  
Sugiyarto Sugiyarto ◽  
Nur Rohman Efendi ◽  
Diah Pratiwi ◽  
Ahmad Dwi Setyawan ◽  
...  
Keyword(s):  
Analgesic Activity ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Chemically Induced

Karika (<em>Carica pubesce</em><em>ns</em>)is a typical plant of the Dieng plateau. Previous study showed that <em>Carica</em><em>pubescens</em>contains chemical compound such as flavonoid that play role in analgesic activity. This study was aimed to determine the analgesic activity of etanolic extracts of karika leaves) in vivo using writhing method. The study was conducted on 25 male mice strain Swiss-Webster (20-30 g, 2-3 months) that were randomly divided into 5 groups. Group I was given distilled water 1 mL (p.o) as negative control, group II was given tramadol 50 mg/kgBW (p.o) as positive control, and group III-V received an etanolic extracts of karika leaves in 3 doses, i.e. 20 mg/kgBW, 40 mg/kgBW, and 80 mg/kgBW (p.o), respectively. Acetic acid 0,5% (v/v) was used as pain inductor.  The writhe was observed within 1 hour. Data analysis was carried out by using one way ANOVA. The result showed that the ethanol extract of leaves Karika <em>(Carica </em><em>pubescens)</em> have activity as an analgesic at a dose of 20 mg / kg, 40 mg / kg and 80 mg / KgBW (p &lt;0.05), chemically induced, where a dose of 80 mg / KgBW (p.o) produce the most high analgesic activity.

scholarly journals Anti-Inflammatory Activity of Ethanol And Fraction of Buni Leaves (Antidesma Bunius L.) on White Rat In Carrageenan Induced Paw Inflammation

1970 ◽  
Vol 7 (5) ◽  
pp. 1-5
Author(s):  
Lintang Kautsar ◽  
Panal Sitorus ◽  
Aminah Dalimunthe
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Group Iii ◽  
Group I ◽  
Anti Inflammatory ◽  
Rat Paw

Objectives: The purpose of this study was to investigate the anti-inflammatory effect ethanol extract and fraction of buni leaves (Antidesma bunius L.) in term of decreased edema volume of male white rat in 1% carrageenan-induced and also to determine the effective dose Design: The design of this study was experimental, where the extraction and fraction of buni leaves were tested for inflammation inhibition value ​​in carrageenan-induced white rats.The inflammatory activity test was divided 6 groups. The Group I (negative control) was given CMC 0.5%, Group II (positive control) was given diclofenac sodium 2,25 mg/kg bw (body weight), while Group III, IV, V and VI were given buni leaves extract and fraction at a dose of 100, 200 and 400 mg/kgbw respectively Interventions: The variable that was intervened in this study was the concentration of extract used Main outcome measure: The main measurement results in this study were to know the extracts and fractions which are capable inhibitor volume of edema in the carrageenan-induced rat paw Results: the anti-inflammatory effects from buni leaves exhibited the most effective activity to reduce edema in the rat paw i.e. EEDB 200 mg / kg bw and FEADB 200 mg / kg bw showing the same value of against positive control Conclusion: ethanol extract and ethyl acetate fraction buni leaves(Antidesma bunius L.) has an effective anti-inflammatory activity at a dose of 200 mg/kg bw.

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