OVARIAN SECRETION RATES OF OESTROGENS, ANDROGENS AND PROGESTERONE IN NORMAL WOMEN AND IN WOMEN WITH PERSISTENT OVARIAN FOLLICLES

1974 ◽  
Vol 77 (3) ◽  
pp. 575-587 ◽  
Author(s):  
F. H. de Jong ◽  
D. T. Baird ◽  
H. J. van der Molen
Keyword(s):  
Menstrual Cycle ◽  
Production Rate ◽  
Follicular Fluid ◽  
Ovarian Follicles ◽  
Dehydroepiandrosterone Sulphate ◽  
Peripheral Plasma ◽  
Normal Women ◽  
Quantitative Contribution ◽  
Secretion Rates ◽  
Venous Plasma

ABSTRACT The concentrations of oestradiol-17β, oestrone, androstenedione, testosterone, dehydroepiandrosterone, dehydroepiandrosterone sulphate and progesterone were measured in ovarian venous plasma from one or both ovaries in 4 normal women during different stages of the menstrual cycle and in 4 women with persistent ovarian follicles. In addition the steroid concentrations in peripheral plasma and follicular fluid were estimated. All steroids mentioned, with the exception of dehydroepiandrosterone sulphate, were secreted by the ovaries. The concentrations of oestradiol-17β, oestrone, androstenedione and progesterone were higher in the venous plasma from the ovary containing the developing follicle or corpus luteum than in venous plasma from the contralateral ovary. There was a good correlation between ovarian secretion of the oestrogenic steroids and androstenedione. Finally, the quantitative contribution of the ovarian secretion to the blood production rates of the androgens and progesterone was calculated. The only contributions exceeding 20 % of the blood production rate were those of progesterone and androstenedione during the second half of the cycle.

CONCENTRATION OF OESTROGENS AND ANDROGENS IN HUMAN OVARIAN VENOUS PLASMA AND FOLLICULAR FLUID THROUGHOUT THE MENSTRUAL CYCLE

1976 ◽  
Vol 71 (1) ◽  
pp. 77-85 ◽  
Author(s):  
K. P. McNATTY ◽  
D. T. BAIRD ◽  
A. BOLTON ◽  
P. CHAMBERS ◽  
C. S. CORKER ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Follicular Fluid ◽  
Venous Blood ◽  
Follicular Phase ◽  
Ovarian Vein ◽  
Preovulatory Follicle ◽  
Peripheral Plasma ◽  
Preovulatory Follicles ◽  
Late Follicular Phase ◽  
Venous Plasma

SUMMARY The concentrations of androstenedione, testosterone, oestrone and oestradiol-17β were measured in peripheral and ovarian venous blood and follicular fluid of women at various stages of the menstrual cycle. The concentration of oestradiol was similar in small follicles (diameter < 8 mm) at all stages of the menstrual cycle and in large follicles (diameter ⩾ 8 mm) except during the mid- and late follicular phase when the concentration reached a peak (∼ 1500 ng/ml). The concentration of androstenedione was lowest in large preovulatory follicles at mid-cycle at a time when the secretion into the ovarian vein was markedly increased. The concentration of testosterone in large follicles (⩾ 8 mm) was unchanged during the follicular phase whereas in small follicles there was a peak at mid-cycle. The rise in the concentration of testosterone and androstenedione at mid-cycle in peripheral plasma may be due to increased secretion by the preovulatory follicle into the ovarian vein. It is suggested that the relatively low concentration of androstenedione in follicular fluid of the preovulatory follicle arises from increased aromatization by granulosa cells in the course of oestrogen synthesis.

CHANGES IN THE CONCENTRATION OF PITUITARY AND STEROID HORMONES IN THE FOLLICULAR FLUID OF HUMAN GRAAFIAN FOLLICLES THROUGHOUT THE MENSTRUAL CYCLE

1975 ◽  
Vol 64 (3) ◽  
pp. 555-571 ◽  
Author(s):  
K. P. McNATTY ◽  
W. M. HUNTER ◽  
A. S. McNEILLY ◽  
R. S. SAWERS
Keyword(s):  
Menstrual Cycle ◽  
Steroid Hormones ◽  
Follicular Fluid ◽  
Follicle Stimulating Hormone ◽  
Secretory Activity ◽  
Follicular Phase ◽  
Hormonal Changes ◽  
Peripheral Plasma ◽  
Before And After ◽  
Late Follicular Phase

SUMMARY The concentrations of FSH, LH, prolactin, oestradiol and progesterone were measured in peripheral plasma and follicular fluid of women throughout the menstrual cycle. With the exception of prolactin, concentrations of pituitary and steroid hormones in follicular fluid correlated with those in peripheral plasma. Follicle-stimulating hormone was present in a greater number of small follicles ( < 8 mm) during or just after the peaks of FSH in peripheral plasma. During the mid-follicular phase the concentration of both FSH and oestradiol in fluid from large follicles ( ≥ 8 mm) was high. During the late follicular phase the large follicles ( ≥ 8 mm) contained high amounts of progesterone in addition to oestradiol, low physiological levels of prolactin, and concentrations of LH and FSH about 30 and 60% respectively of those found in plasma. By contrast no large 'active' follicles ( ≥ 8 mm) were found during the luteal phase although many contained both LH and FSH. Luteinizing hormone was present in a proportion of small follicles ( < 8 mm) during the late follicular and early luteal but not at other stages of the menstrual cycle. It is suggested that a precise sequence of hormonal changes occur within the microenvironment of the developing Graafian follicle; the order in which they occur may be of considerable importance for the growth of that follicle and secretory activity of the granulosa cells both before and after ovulation.

The relationship between prolactin, dehydroepiandrosterone sulphate and testosterone in normally menstruating females

1985 ◽  
Vol 109 (2) ◽  
pp. 173-175 ◽  
Author(s):  
N. C. Wathen ◽  
L. Perry ◽  
S. Hodgkinson ◽  
T. Chard
Keyword(s):  
Menstrual Cycle ◽  
Inverse Relationship ◽  
High Serum ◽  
Serum Prolactin ◽  
Dehydroepiandrosterone Sulphate ◽  
Blood Samples ◽  
Normal Women ◽  
The Relationship ◽  
Circulating Levels

Abstract. Circulating levels of prolactin, dehydroepiandrosterone sulphate (DHEAS) and testosterone (T) were measured in single blood samples from 115 normal women throughout the menstrual cycle. There was an inverse relationship between prolactin and both DHEAS and T. Subjects with high serum prolactin levels had significantly lower levels of DHEAS and T.

scholarly journals Changes in the Concentration of Gonadotrophic and Steroidal Hormones in the Antral Fluid of Ovarian Follicles Throughout the Oestrous Cycle of the Sheep

1981 ◽  
Vol 34 (1) ◽  
pp. 67 ◽  
Author(s):  
KP McNatty ◽  
M Gibb ◽  
C Dobson ◽  
DC Thurley ◽  
JK Findlay
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Ovarian Follicles ◽  
Oestrous Cycle ◽  
Steroid Secretion ◽  
Steroidal Hormones ◽  
Follicle Size ◽  
The Individual ◽  
Secretion Rates ◽  
Venous Plasma

The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, progesterone, androstenedione and oestradiol were determined in the antral fluid of ovarian follicles > 1 mm in diameter as well as in ovarian venous or peripheral venous plasma, or both, from at least four different animals on each day throughout the oestrous cycle of the sheep. The individual steroid hormones in antral fluid were examined in relation to the steroid-secretion rates in ovarian venous plasma, follicle size and the hormone levels in jugular venous plasma.

ADRENAL CONTRIBUTION TO PLASMA OESTROGENS IN ADRENAL DISORDERS

1974 ◽  
Vol 76 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Basil Ho Yuen ◽  
Robert P. Kelch ◽  
Robert B. Jaffe
Keyword(s):  
Vena Cava ◽  
Adrenocortical Adenoma ◽  
Peripheral Plasma ◽  
Group A ◽  
Male Patients ◽  
Group B ◽  
Normal Men ◽  
Adrenal Secretion ◽  
Secretion Rates ◽  
Venous Plasma

ABSTRACT The concentrations of oestrone (Oe1) and oestradiol (Oe2) in adrenal venous and peripheral plasma (inferior vena cava, aorta and/or antecubital vein) were measured by radioimmunoassay in 8 patients in whom an adrenal disorder was suspected (group A) and 5 patients in whom an adrenal disorder was proven (group B). In group A, 6 patients had higher Oe1 concentrations in adrenal than in peripheral plasma, while 5 patients had higher Oe2 concentrations in adrenal venous plasma. In group B, in 3 of 4 patients with primary aldosteronism secondary to an adrenocortical adenoma, both Oe1 and Oe2 concentrations in the adrenal venous plasma from the side of the adenoma were greater than in peripheral plasma. A patient with Cushing's disease showed a similar gradient for Oe1 but not Oe2. The results are consistent with the following conclusions: 1) Both Oe1 and Oe2 are secreted from the adrenal in neoplastic and non-neoplastic disorders in detectable but small amounts. 2) Estimates of maximal adrenal secretion rates in 3 male patients indicate that the adrenal contribution was less than 4 % (Oe1) and less than 2 % (Oe2), of reported blood production rates of oestrogens in normal men.

ACCUMULATION OF LUTEINIZING HORMONE, OESTRADIOL AND ANDROSTENEDIONE BY SHEEP OVARIAN FOLLICLES IN VIVO

1981 ◽  
Vol 91 (1) ◽  
pp. 99-109 ◽  
Author(s):  
K. P. McNATTY ◽  
CAROLYN DOBSON ◽  
MARION GIBB ◽  
LINDA KIEBOOM ◽  
D. C. THURLEY
Keyword(s):  
Antral Follicle ◽  
Ovarian Follicles ◽  
Time Intervals ◽  
Peripheral Plasma ◽  
Temporal Relationships ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
Secretion Rates ◽  
Large Antral Follicle

The temporal relationships between the levels of LH in peripheral plasma and in follicular fluid of ovarian follicles in anaesthetized sheep were investigated for a 10-h period after a single i.m. injection of LH releasing hormone (LH-RH; 100 μg). The ovarian secretion rates of oestradiol and androstenedione and the levels of these steroids accumulating in different sized follicles at varying time-intervals after the LH-RH injection were also compared. The data show that the rates at which pituitary LH enters and leaves the intrafollicular fluid-filled spaces are substantially slower than those of peripheral blood. Two hours after LH-RH injection the levels of LH in plasma had increased from 1 to 200 ng/ml, whereas in the follicle the levels remained at approximately 2 ng/ml. Ten hours after the LH-RH injection, the levels of LH in plasma had returned to basal values (∼1·4 ng/ml) but in both small and large follicles the levels of LH (∼20 ng/ml) were comparable to those present in similar sized follicles 4 h earlier. The data also indicate that more than 90% of the oestradiol produced by a large antral follicle (≥5 mm diameter) probably enters the bloodstream without first accumulating within the follicular antrum. Finally it is concluded that the clearance of the small amount of oestradiol which does accumulate in the follicular antrum is negligible compared with the clearance of this hormone from peripheral plasma.

THE SITE OF SECRETION OF ANDROSTENEDIONE IN NON-PREGNANT WOMEN

1974 ◽  
Vol 63 (1) ◽  
pp. 201-212 ◽  
Author(s):  
D. T. BAIRD ◽  
P. E. BURGER ◽  
G. D. HEAVON-JONES ◽  
R. J. SCARAMUZZI
Keyword(s):  
Menstrual Cycle ◽  
Pregnant Women ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Peripheral Plasma ◽  
Ovulatory Follicle ◽  
Reproductive Life ◽  
Cellular Compartments ◽  
Venous Plasma

SUMMARY The site of secretion of androstenedione in women was investigated by measuring the concentration of androstenedione in peripheral and ovarian venous plasma by radioimmunoassay at different stages of the menstrual cycle. The concentration in peripheral plasma rose from 1·45 ± 0·11 (s.e.m.) ng/ml on day 1 to a peak on the 7 days around mid-cycle (2·29 ± 0·08) before declining gradually during the luteal phase. The concentration of androstenedione in plasma draining ovaries containing the pre-ovulatory follicle or a corpus luteum (33·2 ± 9·0) was significantly higher than in plasma draining the contralateral ovary (10·1 ± 1·6, P < 0·001). In women in whom ovulation was induced with gonadotrophins the concentration of androstenedione in peripheral plasma rose from 1·42 ± 0·21 before treatment to reach maximum levels (2·5–4·5 ng/ml) in the luteal phase. There was a significant (P < 0·001) correlation between the concentration of androstenedione and oestradiol-17β in peripheral (r = 0·50) and ovarian (r = 0·79) venous plasma. These findings indicate that all three cellular compartments in the ovary secrete significant amounts of androstenedione in women during their reproductive life.

Exit of inhibin-like bioactivity from gonadotropin-stimulated prepubertal pig ovaries

1988 ◽  
Vol 117 (1) ◽  
pp. 39-44 ◽  
Author(s):  
M. R. Sairam ◽  
B. R. Downey
Keyword(s):  
Follicular Fluid ◽  
Follicular Development ◽  
Total Content ◽  
Total Activity ◽  
Peripheral Circulation ◽  
Peripheral Plasma ◽  
Venous Circulation ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Venous Plasma

Abstract. Inhibin-like bioactivity (IBA) was assessed by bioassay in the follicular fluid, ovarian venous plasma, and peripheral plasma of immature gilts in which follicular development had been synchronized by administration of pregnant mare serum gonadotropin (PMSG) and hCG. IBA was detectable in the pooled follicular fluid of control animals, but the concentration as well as the total content increased markedly with the growth of the follicle induced by PMSG. About 36–72 h after PMSG treatment, IBA was found consistently in the venous plasma of both ovaries, although in controls as well as after ovulation (i.e. +48 h after hCG) IBA was not detected. The amount of IBA exiting the ovary by way of venous circulation was only a small fraction (< 1%) of the total activity calculated to be in each ovary at a given time. IBA was undetectable in the peripheral plasma at any sampling time. On the basis of these results we could speculate that other routes of entry of inhibin into peripheral circulation may exist in the immature pig and/or that the predominant action of IBA might be within the follicle itself.

The human corpus luteum secretes 5α-pregnane-3,20-dione

1986 ◽  
Vol 111 (1) ◽  
pp. 116-121 ◽  
Author(s):  
Torbjörn Bäckström ◽  
Agneta Andersson ◽  
David T. Baird ◽  
Gunnar Selstam
Keyword(s):  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Luteal Phase ◽  
Follicular Phase ◽  
Ovarian Vein ◽  
Peripheral Plasma ◽  
P Concentration ◽  
Order Of Magnitude ◽  
Human Corpus Luteum ◽  
Venous Plasma

Abstract. A radioimmunoassay for 5α-pregnane-3,20-dione (5α-DHP) in plasma is described. The concentration of 5α-DHP in peripheral plasma during the follicular phase of the menstrual cycle was of the same order of magnitude as that of progesterone (P). During the luteal phase, the plasma 5α-DHP was 8-fold higher than in the follicular phase and about 1/3 of the P concentration. The concentration of 5α-DHP in ovarian venous plasma draining an ovary containing the corpus luteum was 22-fold higher than the concentration in plasma from the contralateral ovarian vein. These results show that the corpus luteum secretes significant amounts of 5α-DHP.

Urinary melatonin, LH, oestradiol, progesterone excretion during the menstrual cycle or in women taking oral contraceptives

1987 ◽  
Vol 116 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Jocelyne Brun ◽  
Bruno Claustrat ◽  
Michel David
Keyword(s):  
Oral Contraceptive ◽  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Oral Contraceptives ◽  
Coefficient Of Variation ◽  
Luteal Phase ◽  
Positive Relationship ◽  
Follicular Phase ◽  
The Other ◽  
Normal Women

Abstract. Nocturnal urinary excretion of melatonin, LH, progesterone and oestradiol was measured by radioimmunoassay in nine normal women during a complete cycle. In addition, these hormonal excretions were studied in two women taking an oral contraceptive. A high within-subject coefficient of variation was observed for melatonin excretion in the two groups. In the nine normal cycling women, melatonin excretion was not decreased at the time of ovulation, but was significantly increased during the luteal phase compared with that of the follicular phase (P < 0.01). These data are consistent with a positive relationship between melatonin and progesterone during the luteal phase. In the two women under an oral contraceptive, melatonin excretion was found within the same range as for the other nine. The results are discussed in terms of pineal investigation in human.

Sign in / Sign up

Export Citation Format

Share Document