scholarly journals Regulation of corpus luteum development and maintenance: specific roles of angiogenesis and action of prostaglandin F2a

2019 ◽  
Author(s):  
A Miyamoto ◽  
K Shirasuna ◽  
T Shimizu ◽  
H Bollwein ◽  
D Schams
Keyword(s):  
Corpus Luteum ◽  
Prostaglandin F2a

10 INFLUENCE OF ADMINISTRATION OF HUMAN CHORIONIC GONADOTROPIN ON DAY 4 AFTER FIXED-TIME AI ON CORPUS LUTEUM SIZE, FUNCTION, AND PREGNANCY RATE IN BEEF COWS

2014 ◽  
Vol 26 (1) ◽  
pp. 119
Author(s):  
J. B. S. Borges ◽  
D. X. Thedy
Keyword(s):  
Corpus Luteum ◽  
Pregnancy Rate ◽  
Fixed Time ◽  
Beef Cows ◽  
Pregnancy Rates ◽  
Control Group ◽  
Chi Square ◽  
Prostaglandin F2a ◽  
Successful Establishment ◽  
Early Rise

Successful establishment of pregnancy in the cow depends on early rise of progesterone (P4) to improve embryo development. The present study was carried out to determine the corpus luteum (CL) function and pregnancy rate following the administration of 1.500 IU of hCG in suckled beef cows on Day 4 after fixed-time AI (FTAI). Crossbred Angus cows (n = 429), 40 to 70 days postpartum, were synchronized using 2 mg of oestradiol benzoate (EB) i.m. and an intravaginal progesterone-releasing device containing 1 g of P4 for 7 days. At device removal, prostaglandin F2a (150 mg of D-cloprostenol) was injected i.m.; 24 h later, 1 mg of EB was given and the FTAI were done 30 to 36 h after EB using semen of just 1 bull. Cows were assigned alternatively to receive 1.500 IU of hCG i.m. (n = 200) or 1 mL of saline i.m. (n = 229) on Day 4 after FTAI. Ovaries were examined by transrectal ultrasonography (n = 71) on Days 4 and 7 to detect the presence and to measure the area of CL. Blood samples were collected on Days 4, 7, 10, 14, and 30 post-oestrus to determine P4 concentration by RIA. Pregnancy diagnoses were done 30 days after FTAI. The effects of treatments on CL area and serum P4 levels were analysed by ANOVA (Proc GLM, SAS Institute Inc., Cary, NC, USA) and pregnancy rates compared by chi-square (Proc CATMOT, SAS). The induction of accessory CL determined on Day 7 was 44% in cows treated with hCG and not observed in the control group. The area of the CL on Day 7 was significantly higher (P < 0.01; 3.37 ± 2.56 v. 2.56 ± 0.22 cm2) in the hCG group. Despite the increase in CL size, the mean levels of P4 on Days 4, 7, 10, 14, and 30 post-AI indicated no difference (P > 0.05) in CL function between control and hCG-treated cows. On Day 30, pregnancy rates were not different (P = 0.08), at 46.7 and 55% for control and hCG-treated cows, respectively. In conclusion, administration of 1.500 IU of hCG on Day 4 post-FTAI did not improve pregnancy rate in suckled beef cows.

Localization of a Prostaglandin F2a Receptor in Bovine Corpus luteum Plasma Membranes

1976 ◽  
Vol 61 (2) ◽  
pp. 605-611 ◽  
Author(s):  
William S. POWELL ◽  
Sven HAMMERSTRoM ◽  
Bengt SAMUELSSON
Keyword(s):  
Corpus Luteum ◽  
Plasma Membranes ◽  
Prostaglandin F2a ◽  
Bovine Corpus Luteum

103 Effects of administration of human chorionic gonadotrophin at Day 5 post-ovulation on development of the original corpus luteum depend on the locational relationship between the original and accessory corpora lutea

2020 ◽  
Vol 32 (2) ◽  
pp. 177
Author(s):  
K. Hazano ◽  
S. Haneda ◽  
M. Matsui
Keyword(s):  
Corpus Luteum ◽  
Repeated Measures ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Intrauterine Environment ◽  
Prostaglandin F2a ◽  
Tissue Area ◽  
Luteal Tissue ◽  
Relationship Of

In cattle, human chorionic gonadotrophin (hCG) is administered at Day 5 post-ovulation to improve fertility. This treatment can induce ovulation of the first-wave dominant follicle (W1DF), from which an accessory corpus luteum (CL) is generated. In addition, hCG has the effect of promoting CL development. It is possible that the locational relationship between the original and accessary CLs influences the effect of hCG on CL development, because the locational relationship of the CLs affects intraovarian blood flow. The present study aimed to clarify whether the locational relationship between the original and accessory CLs influences the effect of hCG on their development. Cross-bred beef heifers (Holstein×Japanese Black, n=56) were used for the present study. The oestrus cycle was synchronized using oestradiol benzoate (EB) and a controlled internal drug release (CIDR)-based program. Briefly, an administration of EB (2mg) with 9-day CIDR insertion was followed by administration of prostaglandin F2a analogue (PGF2a) on the day of CIDR removal, EB (1mg) 1 day after a PGF2a injection, and GnRH 12h after the second EB injection. At Day 5 post-ovulation, the locational relationship between the original CL and the W1DF was confirmed using transrectal ultrasonography (USG), and two groups were defined: ipsilateral group (IG; n=30), in which the CL and the W1DF are in the same ovary, and contralateral group (CG; n=26), in which the CL and the W1DF are in separate ovaries. Moreover, IG and CG were respectively subdivided into two groups, with or without hCG (1500IU) treatment (IG/hCG, n=15; IG without hCG, n=15, and CG/hCG, n=14; CG without hCG, n=12). The diameter and luteal tissue area (i.e. minus the cavity area) of the original CL and the accessory CL were examined at Days 5, 7, and 14, using USG. Two-way repeated-measures ANOVA was used to compare the diameter and luteal tissue area between IG/hCG and IG without hCG, and between CG/hCG and CG without hCG. In CG, the diameter (P&lt;0.01) and luteal tissue area of the original CL (P&lt;0.001) at Day 7 was increased by receiving hCG, while it did not change in IG. The diameter and luteal tissue area of the original CL at Day 14 were not affected by the administration of hCG in either CG or IG. Moreover, for the accessory CL, no difference of the diameter and luteal tissue area was observed between CG and IG. The present study showed that hCG treatment at Day 5 post-ovulation stimulate the growth of the original CL at Day 7, when the original CL and accessory CL are on contralateral sides. Our results suggest that the effect of administration of the hCG at Day 5 post-ovulation on the original CL development depends on the locational relationship between the original and accessory CL (IG or CG). The function of the CL affects the intrauterine environment for embryonic development. Therefore, it is necessary to investigate the effect of the hCG injection at Day 5 on the function of CL (i.e. plasma P4 concentration) in IG and CG, respectively.

Temporal changes in the corpus luteum during early pregnancy reveal regulation of pathways that enhance steroidogenesis and suppress luteolytic mechanisms†

2020 ◽  
Vol 103 (1) ◽  
pp. 70-84
Author(s):  
C H K Hughes ◽  
E K Inskeep ◽  
J L Pate
Keyword(s):  
Retinoic Acid ◽  
Corpus Luteum ◽  
Retinoic Acid Receptor ◽  
Interferon Tau ◽  
Luteal Function ◽  
Prostaglandin F2a ◽  
Chemokine Ligand ◽  
Maternal Recognition Of Pregnancy ◽  
Nanostring Technology

Abstract Although rescue of the corpus luteum (CL) is required for pregnancy, luteal function during maternal recognition of pregnancy remains largely unexplored. CL were collected from pregnant cattle on days 14, 17, 20, and 23, to encompass the maternal recognition of pregnancy period. Next-generation sequencing was used to profile mRNA abundance during this time, while tandem mass spectrometry and nanostring technology were used to profile proteins and miRNA, respectively. A total of 1157 mRNA were differentially abundant, while 27 miRNA changed, and 29 proteins tended to change. mRNA that increased were regulators of interferon signaling and DNA repair, while those that decreased were associated with luteolytic processes, such as calcium signaling and matrix metallopeptidase (MMP) signaling, indicating inhibition of these processes. One of these, MMP12, was regulated by prostaglandin F2A in vitro. mRNA that were maximally abundant on day 20 were primarily associated with immune processes. Two of these, C-C motif chemokine ligand 1 and NFKB inhibitor alpha, were regulated by interferon tau in vitro. MiRNA that increased were predicted to inhibit phosphatidylinositol signaling, while those that decreased may be negative regulators of steroidogenesis. One protein that was greater on day 20 than on day 14 was aldehyde dehydrogenase 1 family member A1 (ALDH1A1), which synthesizes retinoic acid. Pharmacological inhibition of this enzyme, or of retinoic acid receptor signaling, led to suppression of progesterone production in vitro. Overall, these data indicate that there are changes in the CL of pregnancy that are important for continued luteal function.

scholarly journals Interferon-tau promotes luteal endothelial cell survival and inhibits specific luteolytic genes in bovine corpus luteum

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. 559-568 ◽  
Author(s):  
Raghavendra Basavaraja ◽  
Emilia Przygrodzka ◽  
Bartosz Pawlinski ◽  
Zdzislaw Gajewski ◽  
Monika M Kaczmarek ◽  
...  
Keyword(s):  
Life Span ◽  
Cell Survival ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Interferon Tau ◽  
Interferon Stimulated Genes ◽  
Proliferative Effect ◽  
Domestic Ruminants ◽  
Prostaglandin F2a ◽  
Bovine Corpus Luteum

Interferon-tau (IFNT), a maternal recognition of pregnancy (MRP) signals in domestic ruminants, suppresses the release of luteolytic pulses of uterine prostaglandin F2a (PGF2a), thus extending the corpus luteum (CL) life span. We hypothesized that IFNT also exerts anti-luteolytic actions in bovine CL. To examine the direct effects of IFNT on bovine CL, luteal slices and enriched luteal endothelial cells (LECs) were utilized. We found that recombinant ovine IFNT (roIFNT) markedly elevates interferon-associated genes (STAT1, STAT2 and IRF9) and interferon-stimulated genes (ISGs: MX2, ISG15 and OAS1Y) in both models. Furthermore, IFNT time-dependently induced STAT1 phosphorylation in LECs without affecting total STAT1. roIFNT-stimulated viable LECs numbers and the knockdown of protein inhibitor of activated STAT1 (PIAS1) abolished this effect, suggesting that PIAS1 may mediate the proliferative effect of IFNT. IFNT significantly downregulated luteolytic genes such as TGFB1, thrombospondin-1 (THBS1), endothelin-1 (EDN1) and serpin family E member-1 (SERPINE1) in LECs. However, less robust effects were observed in luteal slices. Moreover, PGF2a alone induced THBS1, SERPINE1 and EDN1 mRNA in CL slices whereas in the presence of IFNT, THBS1 and SERPINE1 stimulation was abolished. Collectively, these results indicate that IFNT acts via STAT1- IRF9-dependent and independent pathways and affects diverse luteal functions. Most interestingly, this study suggests the existence of an anti-luteolytic effect of IFNT in bovine CL, namely, inhibiting key PGF2a-induced luteolytic genes. The proliferative effect of IFNT may constitute an additional mechanism that promotes luteal cell survival, thus, extending the luteal life span during early pregnancy in cows.

Das Corpus luteum – ein zelluläres Modellsystem

2009 ◽  
Vol 69 (05) ◽  
Author(s):  
D Pietrowski ◽  
A Walz ◽  
J Becker ◽  
M Sator
Keyword(s):  
Corpus Luteum

Über die Effektivität von DL- im Vergleich zu D-Cloprostenol bei Milchrindern mit einem Corpus luteum periodicum oder Corpus luteum persistens

2005 ◽  
Vol 33 (06) ◽  
pp. 395-403 ◽  
Author(s):  
M. Thumes ◽  
M. Holsteg ◽  
K. Failing ◽  
H. Bostedt ◽  
R. Hospes
Keyword(s):  
Corpus Luteum ◽  
Corpora Lutea ◽  
Klinische Relevanz

Zusammenfassung Ziel der Untersuchung: Überprüfung der Wirksamkeit von DL-Cloprostenol vs. D-Cloprostenol in der Östrusinduktion bei Milchrindern. Probanden und Methoden: Das Probandenkollektiv umfasste 134 Rinder im Durchschnittsalter von 4,0 ± 0,5 Jahren (99 in Laktation, 35 Färsen). Vor alternierender Injektion zweier Cloprostenolpräparate (Gruppe A: DL-Cloprostenol, 500 μg, n = 70; Gruppe B: D-Cloprostenol, 150 μg, n = 64) wurde die Progesteronkonzentration im Serum bestimmt. Gynäkologische Kontrollen erfolgten 0–3 d post injectionem (p. inj.), wobei die als inseminationsfähig beurteilten Probanden (n = 123) am dritten Tag besamt wurden. Ergebnisse: In beiden Gruppen kam es bis zum dritten Tag p. inj. zu einer deutlichen Konsistenzänderung oder Regression der Corpora lutea (p ≤ 0,001). Die Lysis eines C. l. periodicum verlief bei Kühen markanter als bei Färsen (p ≤ 0,017). Insgesamt war D-Cloprostenol dem DL-Cloprostenol hier leicht überlegen. Am dritten Tag p. inj. wiesen 67,1% (A) bzw. 71,9% (B) der Probanden gut ausgeprägte Östrusanzeichen auf. Als inseminationsfähig wurden 94,3% (A) und 89,1% (B) der Tiere eingestuft. Ein geringer Präparateunterschied bestand hinsichtlich des Graviditätsresultates. Bei einem C. l. persistens verlief die Regression weniger progressiv als bei einem C. l. periodicum (p ≤ 0,024). Signifikante Wechselwirkungen zwischen den Einflussfaktoren Präparat und Indikation einerseits sowie für die übrigen gynäkologischen Kriterien andererseits ergaben sich nicht. Bei den Probanden mit prostaglandininduziertem Zyklus nach einem C. l. persistens lag die Graviditätsrate nach der 1. KB deutlich niedriger (31,9%) als bei Tieren nach Lysis eines C. l. periodicum (52,4%, p = 0,08). Schlussfolgerungen und klinische Relevanz: Der Einsatz von D-Cloprostenol erbringt im Wesentlichen die Resultate wie der von DL-Cloprostenol. Eine Überlegenheit konnte jedoch für die Progressivität der lytischen Wirkung des D-Cloprostenols festgestellt werden. Kühe mit C. l. persistens reagierten präparateunabhängig weniger intensiv als solche mit C. l. periodicum.

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