scholarly journals Determination of chemical composition of the Ligularia narynensis root by gas chromatography-mass spectrometry

2019 ◽  
pp. 14-19
Author(s):  
Aliya Nurlybekova ◽  
Yang Ye ◽  
Zharylkasyn Abilov ◽  
Janar Jenis
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Constituents ◽  
Dienoic Acid ◽  
Perennial Herb ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Anticancer Activities ◽  
Root Part ◽  
Chromatography Mass Spectrometry

Ligularia is a medicinally important herb of the family Compositae. Ligularia narynensis is a perennial herb growing in the mountains, rich in sesquiterpenes, triterpenes, lignans, alkaloids, and steroids. In this work chemical constituents of the root part of medicinal plant L. narynensis from Kazakhstan have been determined for the first time. The constituents of the root part of L. narynensis were extracted with hexane and analyzed by gas chromatography – mass spectrometry (GC-MS). Thirty compounds were detected, and their concentrations were determined by the method of normalization of peak areas. Among them, the major components are (9Z,12E)-octadeca-9,12-dienoic acid (16.7%), ethyl (9Z,12Z)-octadeca-9,12-dienoate (11.1%), n-hexadecanoic acid (11.0%), (3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-yl) acetate (9.1%), [(3R)-4,4,6a,6b,8a,11,11,14b-octamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl] acetate (5.1%). Presence of these bioactive constituents may indicate that the plant extract possesses anti-inflammatory, antimicrobial and anticancer activities, which can serve as a basis for the development of new phytopreparations.

Gas Chromatography Mass Spectrometry Application to Investigate of Phytonutrient Different Parts of Lotus

2021 ◽  
pp. 35-40
Author(s):  
Warachate Khobjai ◽  
◽  
Khemjira Jarmkom ◽  
Nakuntwalai Wisidsri ◽  
Surachai Techaoei
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Plant Foods ◽  
Chromatography Mass Spectrometry ◽  
Phytochemical Constituents ◽  
Major Chemical ◽  
Mass Spectrometry Application ◽  
Different Parts

Phytonutrients, also called phytochemicals, are chemicals produced by plants. Foods and vegetables with phytonutrients have antioxidant and anti-inflammatory benefits. The aim of this study was to analyzed 4 parts of 3 lotuses phytochemical constituents by using gas chromatography mass spectrometry. Our results showed that different parts of lotus had a different chemical compound. The major chemical constituents in each extract are 6-Methoxy-2-[p-tolyl]cinchoninic acid (23.92%), Stigmastan-3, 5-diene (6.17%), 5(1H)-Azulenone, 2, 4, 6, 7, 8, 8a-hexahydro-3, 8-dimethyl-4-(1-methylethylidene)-, (8S-cis)- (24.44%), 2-Cyclohexane-1-carboxaldehyde, 2, 6-dimethyl-6-(4-methyl-3-pentennyl (18.15%), Stigmastan-3, 5-diene (24.04%), 2(1H)Naphthalenone, 3, 5, 6, 7, 8, 8a-hexanhydro-4, 8a-dimethyl-6-(1-methylethenyl)-(6.49%), 2-[p-Methoxyphenyl]-8-methylcinchoninic acid (12.98%), Ergosta-4, 6, 22-trien-3, beta, -ol (13.57%), and Stigmastan-3, 5-diene (26.05%). Therefore, Phytonutrients could also provide significant benefits for human’s health that eat plant foods.

scholarly journals Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Yunshuang Fan ◽  
Yamei Li ◽  
Yuanyuan Wu ◽  
Lixin Li ◽  
Yuming Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
Rat Plasma ◽  
Rapid Screening ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Chromatography Mass Spectrometry

Simiao Wan (SMW), an important multiherbal formula used in traditional Chinese medicine, is extensively used to treat rheumatoid arthritis. However, the knowledge of the bioactive components of SMW remains unclear. Thus, gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) were used to analyze the chemical constituents of volatile and nonvolatile extracts of SMW, as well as its absorbed components in rat plasma after oral SMW administration. Identification of several compounds was enabled by comparison of retention times, MS spectra, and MS/MS spectral data with the standard substance and reference materials reported in the literature. In the volatile extracts, GC-MS identified 26 compounds in vitro, three of which observed in blood by GC-MS. In the nonvolatile extracts, LC-MS identified 49 compounds in SMW; 18 compounds containing 7 prototype compounds, 5 metabolites, and 6 unknown compounds were absorbed by blood. The proposed GC-MS and LC-MS method was appropriate not only for the rapid screening and identification of multiple components of an SMW extract but also for screening its bioactive constituents in vivo. The proposed method could be a promising tool for the quality control of other Chinese herbal medicines.

scholarly journals Analysis of the Volatile Components in Selaginella doederleinii by Headspace Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 115 ◽  
Author(s):  
Xian-kui Ma ◽  
Xiao-fei Li ◽  
Jian-yong Zhang ◽  
Jie Lei ◽  
Wei-wei Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Extraction Temperature ◽  
Headspace Solid Phase Microextraction ◽  
Chromatography Mass Spectrometry

Selaginella doederleinii (SD) is a perennial medicinal herb widely distributed in China. In this study, the volatile components of SD from two regions (24 batches), namely Zhejiang and Guizhou, were determined by combining headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME/GC-MS). After investigating different influence factors, the optimal conditions for extraction were as follows: The sample amount of 1 g, the polydimethylsiloxane-divinylbenzene (PDMS-DVB) fiber of 65 µm, the extraction time of 20 min, and the extraction temperature of 100 °C. Based on the above optimum conditions, 58 volatiles compounds, including 20 terpenes, 11 alkanes, 3 alcohols, 6 ketones, 3 esters, 11 aldehydes, 1 ether, 1 aromatic, 1 phenol, and 1 furan, were found and identified in SD. Furthermore, hierarchical cluster analysis and principal component analysis were successfully applied to distinguish the chemical constituents of SD from two regions. Additionally, anethol, zingerone, 2,4-di-tert-butylphenol, ledene, hexyl hexanoate, α-cadinol, phytone, hinesol, decanal, octadecene, cedren, 7-tetradecene, copaene, β-humulene, 2-butyl-2-octenal, tetradecane, cedrol, calacorene, 6-dodecanone, β-caryophyllene, 4-oxoisophorone, γ-nonanolactone, 2-pentylfuran, 1,2-epoxyhexadecane, carvacrol, n-pentadecane, diisobutyl phthalate, farnesene, n-heptadecane, linalool, 1-octen-3-ol, phytane, and β-asarone were selected as the potential markers for discriminating SD from 24 habitats in Zhejiang and Guizhou by partial least squares discrimination analysis (PLS-DA). This study revealed the differences in the components of SD from different regions, which could provide a reference for the future quality evaluation.

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

2018 ◽  
Vol 11 (7) ◽  
pp. 271 ◽  
Author(s):  
Janpen Tangjitjaroenkun
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

Analysis of Volatile Compounds in Flowers of Fraxinax sieboldiana by Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

2012 ◽  
Vol 581-582 ◽  
pp. 1164-1167
Author(s):  
Rong Zheng ◽  
Wei Bin Hu ◽  
Xiao Hong Yang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Chemical Constituents ◽  
Gas Chromatography Mass Spectrometry ◽  
Headspace Solid Phase Microextraction ◽  
Phenylethyl Alcohol ◽  
Chromatography Mass Spectrometry

Objective: To analyse chemical constituents of the volatiles flowers of Fraxinax sieboldiana. Method: The volatiles of bungei were extracted through Headspace solid-phase microextracti, and then the constituents were separated by GC and identified by MS. Result and Conclusion: 22 Compounds were identified. The principal chemical constituents of the volatiles flowers of Fraxinax sieboldiana are Ocimene（44.783%）、α-Tolualdehyde（13.286%）、Linalool(8.816%)、Phenylethyl Alcohol(4.624%)、Benzaldehyde(3.929%)、Cinnamene(3.840%)、α-cis-Ocimene(3.024%)、Farnesene(2.118%)、β-Pyronene(1.673%)、3-Phenylhexane(1.866%)、1,3,8-p-Menthatriene (1.539%)、Formanilide(1.409%) etc,respectively.

scholarly journals GC/MS Analysis of Some Bioactive Constituents from Carthamus lanatus L.

2003 ◽  
Vol 58 (9-10) ◽  
pp. 697-703 ◽  
Author(s):  
Maya Mitova ◽  
Rilka Taskova ◽  
Simeon Popov ◽  
Ralf Günter Berger ◽  
Ulrich Krings ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Artemia Salina ◽  
Sterol Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Aerial Parts ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

AbstractSterols, triterpenes, volatiles, polar and other constituents in aerial parts of Carthamus lanatus were analyzed by gas chromatography-mass spectrometry. Over 90 compounds were identified most of them new for the species. Sitosterol and stigmasterol were the most abundant of 10 sterols identified in the sterol fraction. Taraxasterol, α- and β-amyrine prevailed in the triterpene fraction. Volatiles, sterols and a fraction of the dichloromethane extract showed strong cytotoxicity (Artemia salina assay).

scholarly journals Gas Chromatography- Mass Spectrometry Analysis of Phthalide Composition of Celery oil from Apium graveolens L. cultivated in Nigeria.

2021 ◽  
Vol 46 (2) ◽  
Author(s):  
E. A. Adelakun ◽  
C. Achuenu ◽  
E. C. Ngurukwem ◽  
D. J. Chuwang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Mass Spectrometry Analysis ◽  
Apium Graveolens ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Medicinal Uses ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
Leaf Oil

Phthalides are important bioactive constituents of celery plants. The medicinal uses of celery and their characteristic aroma have been linked to the phthalides. In this report, the leaf and stalk oils of celery were extracted either by hydrodistillation or maceration in water and the phthalide constituents of the oils were characterized by gas chromatography-mass spectrometry (GC-MS) analysis. The leaf oil consists mainly of dihydroxyphthalides while the volatile phthalides are the main phthalides in the stalk oil. The phthalide content of the leaf oil and the stalk oil are 39.8% and 29.95% respectively. The phthalides identified in the leaf oil are the isomeric compounds of senkyunolide I and senkyunolides (J & N), while the major phthalide constituents of the stalk oil include 3-n- butylphthalide, sedanolide, neocnidilide and sedanenolide/senkyunolide A. The results suggest that the Nigerian celery plant could be considered a good source of phthalides which are known for their nutraceutical properties such as anti-oxidant, antitumor, anti- platelet aggregation, hypotensive, hypoglycemic and hypolipidemic properties. Further phytochemical and pharmacological evaluation of the locally cultivated celery are required in order to exploit the medicinal potentials of the plant for applications in pharmaceutical and cosmetic industries.

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