scholarly journals Identification of the Chemical Constituents in Simiao Wan and Rat Plasma after Oral Administration by GC-MS and LC-MS

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Yunshuang Fan ◽  
Yamei Li ◽  
Yuanyuan Wu ◽  
Lixin Li ◽  
Yuming Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Constituents ◽  
Herbal Medicines ◽  
Rat Plasma ◽  
Rapid Screening ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Chromatography Mass Spectrometry

Simiao Wan (SMW), an important multiherbal formula used in traditional Chinese medicine, is extensively used to treat rheumatoid arthritis. However, the knowledge of the bioactive components of SMW remains unclear. Thus, gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–mass spectrometry (LC-MS) were used to analyze the chemical constituents of volatile and nonvolatile extracts of SMW, as well as its absorbed components in rat plasma after oral SMW administration. Identification of several compounds was enabled by comparison of retention times, MS spectra, and MS/MS spectral data with the standard substance and reference materials reported in the literature. In the volatile extracts, GC-MS identified 26 compounds in vitro, three of which observed in blood by GC-MS. In the nonvolatile extracts, LC-MS identified 49 compounds in SMW; 18 compounds containing 7 prototype compounds, 5 metabolites, and 6 unknown compounds were absorbed by blood. The proposed GC-MS and LC-MS method was appropriate not only for the rapid screening and identification of multiple components of an SMW extract but also for screening its bioactive constituents in vivo. The proposed method could be a promising tool for the quality control of other Chinese herbal medicines.

In vitro and in vivo studies of androst-4-ene-3,6,17-trione in horses by gas chromatography-mass spectrometry

2009 ◽  
Vol 24 (7) ◽  
pp. 744-751 ◽  
Author(s):  
Gary N. W. Leung ◽  
Francis P. W. Tang ◽  
Terence S. M. Wan ◽  
Colton H. F. Wong ◽  
Kenneth K. H. Lam ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
In Vivo Studies ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals Determination of chemical composition of the Ligularia narynensis root by gas chromatography-mass spectrometry

2019 ◽  
pp. 14-19
Author(s):  
Aliya Nurlybekova ◽  
Yang Ye ◽  
Zharylkasyn Abilov ◽  
Janar Jenis
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Constituents ◽  
Dienoic Acid ◽  
Perennial Herb ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Constituents ◽  
Anticancer Activities ◽  
Root Part ◽  
Chromatography Mass Spectrometry

Ligularia is a medicinally important herb of the family Compositae. Ligularia narynensis is a perennial herb growing in the mountains, rich in sesquiterpenes, triterpenes, lignans, alkaloids, and steroids. In this work chemical constituents of the root part of medicinal plant L. narynensis from Kazakhstan have been determined for the first time. The constituents of the root part of L. narynensis were extracted with hexane and analyzed by gas chromatography – mass spectrometry (GC-MS). Thirty compounds were detected, and their concentrations were determined by the method of normalization of peak areas. Among them, the major components are (9Z,12E)-octadeca-9,12-dienoic acid (16.7%), ethyl (9Z,12Z)-octadeca-9,12-dienoate (11.1%), n-hexadecanoic acid (11.0%), (3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-yl) acetate (9.1%), [(3R)-4,4,6a,6b,8a,11,11,14b-octamethyl-1,2,3,4a,5,6,7,8,9,10,12,12a,14,14a-tetradecahydropicen-3-yl] acetate (5.1%). Presence of these bioactive constituents may indicate that the plant extract possesses anti-inflammatory, antimicrobial and anticancer activities, which can serve as a basis for the development of new phytopreparations.

scholarly journals Gas Chromatography–Mass Spectrometry Profiling of Volatile Compounds Reveals Metabolic Changes in a Non-Aflatoxigenic Aspergillus flavus Induced by 5-Azacytidine

Toxins ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 57 ◽  
Author(s):  
Fengqin Song ◽  
Qingru Geng ◽  
Xuewei Wang ◽  
Xiaoqing Gao ◽  
Xiaona He ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Volatile Compounds ◽  
Aspergillus Flavus ◽  
Cyclopiazonic Acid ◽  
Metabolic Changes ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Aspergillus flavus is one of the most opportunistic pathogens invading many important oilseed crops and foodstuffs with such toxic secondary metabolites as aflatoxin (AF) and Cyclopiazonic acid. We previously used the DNA methylation inhibitor 5-azacytidine to treat with an AF-producing A. flavus A133 strain, and isolated a mutant (NT) of A. flavus, which displayed impaired abilities of AF biosynthesis and fungal development. In this study, gas chromatography–mass spectrometry (GC-MS) analysis was used to reveal the metabolic changes between these two strains. A total of 1181 volatiles were identified in these two strains, among which 490 volatiles were found in these two strains in vitro and 332 volatiles were found in vivo. The NT mutant was found to produce decreasing volatile compounds, among which most of the fatty acid-derived volatiles were significantly downregulated in the NT mutant compared to the A133 strain, which are important precursors for AF biosynthesis. Two antioxidants and most of the amino acids derived volatiles were found significantly upregulated in the NT mutant. Overall, our results reveal the difference of metabolic profiles in two different A. flavus isolates, which may provide valuable information for controlling infections of this fungal pathogen.

scholarly journals ANALISIS GC-MS (GAS CHROMATOGRAPHY - MASS SPECTROMETRY) EKSTRAK METANOL DARI UMBI RUMPUT TEKI (Cyperus rotundus L.)

PHARMACON ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 849
Author(s):  
Ellen Hotmian ◽  
Elly Suoth ◽  
Fatimawali Fatimawali ◽  
Trina Tallei
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Cyperus Rotundus ◽  
Gas Chromatography Mass Spectrometry ◽  
The Public ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

ABSTRACTThe Nut Grass Tuber (Cyperus rotundus L.) is a plant that is believed by the public to cure several diseases. According to a study conducted in vivo and in vitro, the extract the tuber root has many potentials such as anticancer, anti-inflammatory, antibacterial, etc. This study aims to determine the polar bioactive compounds contained in the bulb tubers. The method used was the extraction of nut tuber dry powder using methanol as a solvent by maceration process and then analyzed using gas chromatography - mass spectrometry (GC-MS) to obtain information on the content of the tubers The results of GC-MS analysis from this study indicate that there are 177 possible components of the compound extracted using methanol. The results also showed that there were three possible polar bioactive compounds at the highest peak of the GC-MS analysis, namely 7-Isopropenyl-1,4a-dimethyl-4,4a, 5,6, 7,8-hexahydro-3H-naphthalen-2-one, 1 (2H) -Naphthalenone, 3,4,4a, 5,6,7-hexahydro-4a, 5-dimethyl-3- (1-methylethenyl) -, [3S- (3a, 4aa, 5a)] -, and 2 (1H) Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6- (1-methylethenyl) -.Keywords         : Methanolic extract, nut grass tuber, Cyperus rotundus L., GC-MS   ABSTRAKUmbi Rumput Teki (Cyperus rotundus L.) merupakan tumbuhan yang dipercayai masyarakat dapat menyembuhkan beberapa penyakit. Menurut sebuah penelitian yang dilakukan secara in vivo maupun in vitro, kandungan ekstrak umbi rumput teki memiliki banyak potensi seperti antikanker, antiinflamasi, antibakteri, dll. Penelitian ini bertujuan untuk mengetahui senyawa-senyawa bioaktif polar yang terkandung pada umbi rumput teki. Metode yang digunakan yaitu ekstraksi serbuk kering umbi rumput teki menggunakan pelarut metanol dengan proses maserasi kemudian dianalisis menggunakan kromatografi gas – spektrometri massa (GC-MS) untuk mendapatkan informasi kandungan dalam umbi rumput teki. Hasil analisis GC-MS dari penelitian ini menunjukkan bahwa terdapat 177 kemungkinan komponen senyawa yang diekstraksi menggunakan pelarut methanol. Hasil penelitian juga menunjukkan bahwa terdapat tiga kemungkinan senyawa bioaktif polar pada puncak tertinggi hasil analisis GC-MS, yaitu 7-Isopropenyl-1,4a-dimethyl-4,4a,5,6,7,8-hexahydro-3H-naphthalen-2-one, 1(2H)-Naphthalenone, 3,4,4a,5,6,7-hexahydro-4a,5-dimethyl-3-(1-methylethenyl)-, [3S-(3a,4aa,5a)]-, dan 2(1H)Naphthalenone, 3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6-(1-methylethenyl)-. Kata kunci      : Ekstrak metanol, umbi rumput teki, Cyperus rotundus L., GC-MS

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