Second-generation polymerase chain reaction (PCR) and DNA microarrays for in vitro and in situ study of foodborne pathogens

2013 ◽  
pp. 193-201
Author(s):  
K. Rantsiou ◽  
L. Cocolin
Keyword(s):  
Polymerase Chain Reaction ◽  
Foodborne Pathogens ◽  
Dna Microarrays ◽  
Second Generation ◽  
Chain Reaction ◽  
In Situ Study ◽  
Polymerase Chain

scholarly journals CMV and Transplant-Related Coronary Atherosclerosis: An Immunohistochemical, In Situ Hybridization, and Polymerase Chain Reaction In Situ Study

2000 ◽  
Vol 13 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Nadia V Sambiase ◽  
Maria de Lourdes Higuchi ◽  
Gerard Nuovo ◽  
Paulo S Gutierrez ◽  
Alfredo I Fiorelli ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Coronary Atherosclerosis ◽  
Chain Reaction ◽  
In Situ Study ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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