Eco-friendly wood-biofungicidal and antibacterial activities of various Coccoloba uvifera L. leaf extracts: HPLC analysis of phenolic and flavonoid compounds

Aqueous, acetone, and ethanol extracts of Coccoloba uvifera L. (Polygonaceae) leaves were assessed for their antibacterial and antifungal activities. The fungal pathogens Fusarium culmorum, Rhizoctonia solani, and Botrytis cinerea were isolated from strawberry plants, and they were molecularly identified through internal transcribed spacers (ITS) sequence analysis. Wood treated with ethanol extract at 3% showed the highest inhibition of R. solani, B. cinerea, and F. culmorum growth, with mycelial growth inhibited by 64.4%, 100%, and 38.5%, respectively. Moderate growth inhibition was found against the plant pathogenic bacteria Agrobacterium tumefaciens, Pectobacterium carotovorum subsp. carotovorum, Erwinia amylovora, Ralstonia solanacearum, Pectobacterium atrosepticum, and Dickeya solani. High-performance liquid chromatography analysis identified the phenolic and flavonoid compounds in the extracts. Regarding phenolic acid compounds, benzoic, ellagic, gallic, and o-coumaric acids were found as the main compounds in ethanol, acetone, and aqueous extracts. Regarding flavonoids, rutin, myricetin, and quercetin were identified in aqueous, acetone, and ethanol extracts. The results suggesting that the extracts can be used as environmentally friendly bioagents.

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Study of Potential Phenolic Compounds from Stems of Dendrophthoe Falcata (Loranthaceae) Plant as Antioxidant and Antimicrobial Agents

Oriental Journal Of Chemistry ◽

10.13005/ojc/340515 ◽

2018 ◽

Vol 34 (5) ◽

pp. 2342-2349 ◽

Cited By ~ 2

Author(s):

Sri Atun ◽

Sri Handayani ◽

Anna Rakhmawati ◽

Nur Aini Purnamaningsih ◽

Bian Ihda An Naila ◽

...

Keyword(s):

Phenolic Compounds ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Diffusion Method ◽

Total Phenolic ◽

Flavonoid Compounds ◽

Dpph Method ◽

Phenolic And Flavonoid ◽

Phenolic And Flavonoid Compounds ◽

Dendrophthoe Falcata

Dendrophthoe falcata is a hemiparasitic plant that has been used in traditional medicine.The study was conducted to identify the total phenolic and flavonoid compounds, to test antioxidant, and antimicrobial activity of ethanol extract stems of D. falcata parasite on Melia azedarach host tree. The dry powder stems of D. falcata was extracted with ethanol. The ethanol extract was subsequently partitioned successively using n-hexane, chloroform, and ethyl acetate. Each fraction was analyzed by a quantitative phenolic and flavonoid content with spectrophotometer method, and tested as antioxidant and antimicrobial activites. Antioxidant activity was performed by 2,2-diphenyl-1-picrilhidrazil (DPPH) method, while antimicrobial assay used pathogenic bacteria by disk-diffusion method. The results concluded that the stem of D. falcata plant showed a high content of phenolic and flavonoid compounds, very high antioxidant and moderate antimicrobial activities. It was also found that stems of D. falcata contain potential phenolic compounds that can be used as natural antioxidants and the treatment of various infections caused by microbes.

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