scholarly journals Efficient analysis of monosaccharides and oligosaccharides from hydrolyzed hemicellulose of Spartina anglica

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 7628-7639
Author(s):  
Rusheng Xie ◽  
Meng Li ◽  
Suixiang Ma ◽  
Jian Liu ◽  
Minnan Long
Keyword(s):  
High Performance Liquid Chromatography ◽  
Coastal Erosion ◽  
Mobile Phase ◽  
High Performance ◽  
Enzymatic Digestion ◽  
Spartina Anglica ◽  
Chromatography Method ◽  
The World ◽  
Liquid Chromatography Method ◽  
Elution Gradient

Spartina anglica, a plant that controls coastal erosion, is widely distributed throughout the world and is rich in cellulose, hemicellulose, and lignin. The hemicellulose from Spartina anglica can be extracted and hydrolyzed into monosaccharides and xylooligosaccharides under acid or enzymatic digestion conditions. In this study, an effective PMP(1-phenyl-3-methyl-5-pyrazolone)-derivatized HPLC (High performance liquid chromatography) method was developed for monitoring monosaccharides and xylooligosaccharides of Spartina anglica. With phosphate buffer (0.04 M, pH 8.06) as mobile phase A, and acetonitrile as mobile phase B, in which the elution gradient was set as A:B/79:21, the monosaccharides (glucose, xylose and arabinose) and xylooligosaccharides (xylobiose, xylotriose, xylotetraose, xylopentaose, xylohexaose) could be separated completely using the C18 column. This provides an economical, rapid, and efficient method for process monitoring in the bioconversion of Spartina anglica.

scholarly journals Determination of dyes and marker in diesel using high performance liquid chromatography

Chemija ◽  
2018 ◽  
Vol 29 (2) ◽  
Author(s):  
Audrius Markevičius ◽  
Audrius Zolumskis ◽  
Audrius Sadaunykas ◽  
Birutė Knašienė ◽  
Adrian Vicent Claramunt ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Direct Injection ◽  
Chromatography Method ◽  
Visible Wavelength Range ◽  
Liquid Chromatography Method ◽  
Elution Gradient

A fast, precise and accurate high performance liquid chromatography method has been developed for the determination of dyes (Solvent Red 19 and Solvent Blue 35) and a marker (Solvent Yellow 124) in diesel. Separation was carried out on a 250 × 4.60 mm Agilent Zorbax Rx-SIL column (5 µm particle size). Detection was done in a visible wavelength range. The best performance of fuel dye separation and the shortest retention times were achieved when using hexane, toluene and ethyl acetate as a mobile phase. During this research the eluent composition and the elution gradient were optimized consequently that helped to perform the analysis within 15 min. The developed method was applied for the analysis of real samples of dyed diesel fuel. Preparation of the samples for the analysis simply consisted of filtering through a 0.45 µm filter previous to direct injection of the sample into the HPLC system for analysis.

FORCED DEGRADATION STUDY OF ELETRIPTAN BY USING ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (UHPLC)

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (07) ◽  
pp. 14-21
Author(s):  
S. Sahu ◽  
◽  
R.M Singh ◽  
S.C. Mathur ◽  
D. K Sharma ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Forced Degradation ◽  
Chromatography Method ◽  
Degradation Study ◽  
Liquid Chromatography Method ◽  
Isocratic Mode

A simple, fast, precise and accurate ultra high performance liquid chromatography method was developed for degradation study of eletriptan hydrobromide (EH) under exaggerated conditions. An Inertsil ODS C18 (250 x 4.6 mm, 5µm) column in isocratic mode was used with mobile phase comprising of water, methanol and trifluoroacetic acid mixed in the ratio 55:45:0.1 % V/V/V, maintained at pH 3.5. The flow rate was set at 0.4 mL per minute with UV detection at 225 nm. The retention time of EH was found to be 3.7 minutes. Linearity for EH was found in the range of 3.5- 200 µg per mL and percentage recoveries were obtained in the range of 100.2 % to 100.6 %. The method was capable of resolving all degradants and principle component in sample. The proposed method is accurate, precise, selective, reproducible, and rapid for detection of degradation of eletriptan hydrobromide.

DEVELOPMENT AND VALIDATION OF THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE ESTIMATION OF GUAIFENESIN IN METHOCARBAMOL API AND ITS PHARMACEUTICAL DOSAGE FORMS

2018 ◽  
Vol 11 (5) ◽  
pp. 401
Author(s):  
Mannem Durga Babu ◽  
Kesana Surendrababu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Solution Stability ◽  
Chromatography Method ◽  
Pharmaceutical Dosage Forms ◽  
Liquid Chromatography Method

Objective: The objective of the study was to develop and validate a novel, specific, precise, and simple reversed-phase high-performance liquid chromatography method for the estimation of guaifenesin present in methocarbamol API and its pharmaceutical dosage forms. Methods: The baseline separation for methocarbamol and guaifenesin was achieved by utilizing a Inertsil ODS C18 (250 mm × 4.6 mm) 5 μm column particle size and an isocratic elution method. The mobile phase contains a mixture of water and acetonitrile in the ratio of 70:30 v/v, respectively. The flow rate of the mobile phase was 1.0 mL/min with a column temperature of 25°C and detection wavelength at 272 nm. The method was validated for a limit of detection (LOD), limit of quantification (LOQ), linearity, accuracy, and reproducibility with the help of the exhibit and simulated samples. Results: The LOD for guaifenesin was 0.62 μg/mL. The LOQ for guaifenesin was 1.87 μg/mL. The correlation coefficient obtained for impurity was >0.99. The recovery was obtained for impurity was 106.56% at 50%, 95.20% at 100%, and 100.45% at 150%. In tablet analysis, we can found 0.26% (<0.5%). Conclusion: The developed method was validated as per the ICH guidelines with respect to specificity, precision, linearity, accuracy, LOD and quantification, ruggedness, robustness, and solution stability.

Determination of Chloramphenicol Residual in Freshwater Fish by HPLC

2013 ◽  
Vol 781-784 ◽  
pp. 1708-1711 ◽  
Author(s):  
Yi Qing Yang ◽  
Qian Wang ◽  
Qiao Wang ◽  
Shi Jie Yan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Freshwater Fish ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Chromatography Method ◽  
Average Recovery ◽  
Liquid Chromatography Method

HPLC (High Performance Liquid Chromatography) method for chloramphenicol residues in freshwater fish was developed. The samples were extracted with ethyl acetate. The LC was performed on a C18 column, mobile phase consisted of methanol-water (45:55 v/v) and eluted at 0.2mL/min at 20°C, inject volume was 20μl. The linear rang for chloramphenicol residual is within 0.5-20μg/mL, the limit of detection is 0.01ng/g. The average recovery is 80.06%-92.71%, and the RSD is 1.46%-5.61%. This method is employed to analyze freshwater fishes, such as catfish, chub and carp.

Quantification of Pyrazinamide in Human Plasma by Validated High Performance Liquid Chromatography Method

2021 ◽  
Vol 15 (10) ◽  
pp. 2896-2899
Author(s):  
Waleed Arshad ◽  
Naseem Saud Ahmad ◽  
Abdul Muqeet Khan ◽  
Iram Imran ◽  
Qura- Tul-Ain ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Mobile Phase ◽  
High Performance ◽  
Ultra Violet ◽  
Chromatography Method ◽  
Ultraviolet Detector ◽  
Relative Standard ◽  
Liquid Chromatography Method

Objective: To be able to accurately determine the quantity of Pyrazinamide (PZA) in different tablet preparations and human plasma using an Ultra violet detector equipped high performance liquid chromatography (HPLC). Study Design: Experimental study Place and Duration of Study: Department of Bioequivalence Studies, University of Veterinary and Animal Sciences Lahore and the Department of Pharmacology, University of Health Sciences, Lahore the from 1st April 2017 to 31st March 2018. Methodology: Two mobile phases were used, the first compromised of disodium hydrogen phosphate buffer having a pH of 6.8 and acetonitrile in the proportion of (95:5) and the second was a combination of aforesaid substances in equivalent proportion (50:50 v/v). The gradient for the first 5 min was exclusively Mobile phase “a” after which 5-6 min Mobile phase “b” was raised from 0 to 100% and was kept at 100% till the completion of the cycle. The flow of mobile phase was kept at 1000 µl/min. Determination of PZA was done using a ultraviolet detector at a wavelength of 238 nm. Amount of sample injected was 40 μl. Procedure was done by using Shizmadu Chromatographic System, Japan equipped with a SIL-20AC HT auto-sampler, SPD-M20A, CTO 20 AC, a LC-20AT VP pump, and CBM 20A controller unit. A C18 column was used as well. Results: Retention time of PZA was 6.1±2%. Precision was 0.46 to 2.20% relative standard deviation for intra assay and for inter assay we obtained 0.29 to 34.45% RSD for all quality control levels. The overall recovery of PZA was 96.75%. Conclusion: High selectivity for PZA was seen and no other spikes from drugs present in FDC regimen were observed at the time when PZA is detected in blank plasma samples Key words: Chromatography, High pressure liquid. Pyrazinamide. Tuberculosis

scholarly journals HPLC Estimation of Flavanoid (quercetin) of leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia

2021 ◽  
Vol 10 (4) ◽  
pp. 220-224
Author(s):  
Praveen Garg ◽  
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Tinospora Cordifolia ◽  
Ocimum Sanctum ◽  
Chromatography Method ◽  
High Antioxidant Activity ◽  
Liquid Chromatography Method

High performance liquid chromatography method is used for the estimation of flavanoid (quercetin) in leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia. The dried leaves and stem of both plants were extracted with methanol and ethanol solvent. The concentration of quercetin of methanolic and ethanolic leaves and stem extracts of plants performed by using acetonitrile and methanol 50:50 (V/V) solutions as mobile phase and used a flow rate of 1ml per min and absorbance at 256 nm. We conclude in this study that Ocimum sanctum and Tinospora cordifolia has significant amount of flavanoid (quercetin), which indicate the high antioxidant activity of both plants and used for further study

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