scholarly journals Eficacia de soluciones salinas modificadas para la supervivencia de plerocercoides Tetraphyllidea de concha de abanico (Argopecten purpuratus)

2021 ◽  
Vol 32 (3) ◽  
pp. e20390
Author(s):  
Teresa Castro ◽  
Enrique C. Mateo ◽  
César A. Peña

El objetivo de este estudio fue obtener una solución salina modificada que permita una supervivencia más prolongada para el estudio morfológico de plerocercoides Tetrafilideos de la concha de abanico Argopecten purpuratus. Se evaluaron dos temperaturas: T-1, temperatura ambiente y T-2, temperatura constante de 0.5 °C y cinco soluciones: A = SSF (solución salina fisiológica – SSF – al 0.85%), B = ¼ AM (agua de mar) + ¾ SSF, C = ½ AM+ ½ SSF, D = ¾ AM + ¼ SSF, E = AM. Se utilizaron 100 larvas vivas recién extraídas de gónadas de A. purpuratus (10 por grupo experimental) durante 13 días. La mayor supervivencia se observó en las soluciones B y C del T-1, donde permanecieron vivos cuatro plerocercoides hasta el día 10 y tres hasta el día 12, respectivamente. Con excepción de la solución A, las larvas mantuvieron su actividad y forma normal en el resto de las soluciones hasta el día 6. Este trabajo demuestra que las soluciones salinas usuales (0.75 a 0.9%) pueden no ser óptimas para mantener plerocercoides marinos vivos y que una salinidad que se aproxima al agua de mar es más apropiada.

Author(s):  
G.C. Bellolio ◽  
K.S. Lohrmann ◽  
E.M. Dupré

Argopecten purpuratus is a scallop distributed in the Pacific coast of Chile and Peru. Although this species is mass cultured in both countries there is no morphological description available of the development of this bivalve except for few characterizations of some larval stages described for culture purposes. In this work veliger larvae (app. 140 pm length) were examined by the scanning electron microscope (SEM) in order to study some aspects of the organogenesis of this species.Veliger larvae were obtained from hatchery cultures, relaxed with a solution of MgCl2 and killed by slow addition of 21 glutaraldehyde (GA) in seawater (SW). They were fixed in 2% GA in calcium free artificial SW (pH 8.3), rinsed 3 times in calcium free SW, and dehydrated in a graded ethanol series. The larvae were critical point dried and mounted on double scotch tape (DST). To permit internal view, some valves were removed by slightly pressing and lifting the tip of a cactus spine wrapped with DST, The samples were coated with 20 nm gold and examined with a JEOL JSM T-300 operated at 15 KV.


Author(s):  
Iván Loaiza ◽  
Gudrun De Boeck ◽  
Juan Alcazar ◽  
Diego Campos ◽  
Susana Cárdenas‐Alayza ◽  
...  

2003 ◽  
Vol 143 (2) ◽  
pp. 327-338 ◽  
Author(s):  
N. Nevejan ◽  
V. Courtens ◽  
M. Hauva ◽  
G. Gajardo ◽  
P. Sorgeloos

Aquaculture ◽  
1995 ◽  
Vol 138 (1-4) ◽  
pp. 49-60 ◽  
Author(s):  
C Riquelme ◽  
G Hayashida ◽  
N Vergara ◽  
A Vasquez ◽  
Y. Morales ◽  
...  

Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 490 ◽  
Author(s):  
Alex Alcántara-Rubira ◽  
Víctor Bárcena-Martínez ◽  
Maribel Reyes-Paulino ◽  
Katherine Medina-Acaro ◽  
Lilibeth Valiente-Terrones ◽  
...  

Causative species of Harmful Algal Bloom (HAB) and toxins in commercially exploited molluscan shellfish species are monitored weekly from four classified shellfish production areas in Perú (three in the north and one in the south). Okadaic acid (OA) and pectenotoxins (PTXs) were detected in hand-picked cells of Dinophysis (D. acuminata-complex and D. caudata) and in scallops (Argopecten purpuratus), the most important commercial bivalve species in Perú. LC-MS analyses revealed two different toxin profiles associated with species of the D. acuminata-complex: (a) one with OA (0.3–8.0 pg cell−1) and PTX2 (1.5–11.1 pg cell−1) and (b) another with only PTX2 which included populations with different toxin cell quota (9.3–9.6 pg cell−1 and 5.8–9.2 pg cell−1). Toxin results suggest the likely presence of two morphotypes of the D. acuminata-complex in the north, and only one of them in the south. Likewise, shellfish toxin analyses revealed the presence of PTX2 in all samples (10.3–34.8 µg kg−1), but OA (7.7–15.2 µg kg−1) only in the northern samples. Toxin levels were below the regulatory limits established for diarrhetic shellfish poisoning (DSP) and PTXs (160 µg OA kg−1) in Perú, in all samples analyzed. This is the first report confirming the presence of OA and PTX in Dinophysis cells and in shellfish from Peruvian coastal waters.


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