scholarly journals Influence of liposomal drug on the intensity of proteins oxide modification processes in subclinical mastitis of cows

2021 ◽  
Vol 23 (2) ◽  
pp. 3-7
Author(s):  
V. A. Chepurna ◽  
◽  
T. M. Suprovych ◽  
O. I. Vishchur ◽  
V. P. Mizik ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Somatic Cells ◽  
Experimental Studies ◽  
Study Drug ◽  
Subclinical Mastitis ◽  
Oxidative Modification ◽  
Control Group ◽  
Antioxidant Protection ◽  
Liposomal Drug ◽  
Intracisternal Injection

The article contains the experimental studies of the liposomal drug based on plant raw materials ― hypericum (Hypericum perforatum L.) effect on the intensity of oxidative modification of proteins (OMP) in the blood and milk of cows with subclinical mastitis. Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP370 and ketone-derived OMP430, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals. In the milk of sick cows, the content of derivatives OMP370 and OMP430 was1.99 and 2.29 times higher, respectively, than in animals of the control group. At the beginning of the study sick cows’ milk was recorded a significantly low value of the activity of the enzymatic link of antioxidant protection ― superoxide dismutase. At the same time, a 2.6-fold (P<0.001) increase in the number of somatic cells was noted compared to their number in the milk of clinically healthy cows. Intracisternal injection of liposomal drug to cows caused a decrease in the intensity of oxidative processes. In the blood of sick cows the content of aldehyde derivatives OMP370 on the 9th day of the experiment was 23.1% (P<0.05) less than before the drug, and in milk the content of OMP370 decreased by 61.8% (P<0.01). Similar changes were observed with respect to the level of ketone derivatives. In particular, on the 9th day of the experiment, the content of OMP430 decreased by 11.7% (P<0.05) compared with its value in the blood of sick animals before the introduction of the study drug, and in milk it decreased by 64.2% (P<0.01). During the treatment on the 9th day of the experiment, the number of somatic cells in milk decreased by 41.8% (P<0.01). In the course of treatment on the 3rd and 9th day there was a tendency to increase superoxide dismutase activity in the milk of sick cows compared with the beginning of the experiment. Thus, intracisternal injection of liposomal drug to cows with subclinical mastitis leads to a decrease in aldehyde and ketone derivatives of proteins oxidative modification in serum and milk. At the same time, an increase in the activity of the enzymatic link of antioxidant protection and a decrease in the number of somatic cells in the milk of cows were recorded.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

scholarly journals STUDY ON THE EFFECTIVENESS OF INTRACISTERNAL ANTIBIOTIC TREATMENT FOR THE CONTROL OF MASTITIS IN GOATS

2020 ◽  
Vol 23 (1) ◽  
pp. 37-40
Author(s):  
K. Hristov ◽  
R. Pepovich ◽  
B. Nikolov
Keyword(s):  
Post Partum ◽  
Somatic Cells ◽  
Treatment Method ◽  
Subclinical Mastitis ◽  
Control Group ◽  
Dry Period ◽  
Selective Treatment ◽  
Intramammary Infections ◽  
Selective Approach ◽  
The Cost

The aim of this study is to investigate the healing and prophylactic effect of drying-off antibiotic therapy using the selective and non-selective approach of administration of the antibiotics. First experimental group at the start of the dry period were treated non-selectively (all halves) intra-cisternally with antibiotics. In the second group, we only treated those halves that were diagnosed with subclinical mastitis. The control group of 9 goats was not treated. The number of somatic cells in treated groups after birth decreased from 2586 x 10³/ mL to 560 x 10³/mL in the first group and from 1978 x 10³/mL to 526 x 10³/mL in the second. Post partum, 10% of the halves were found to have subclinical mastitis. A healing effect was achieved in 25% (n=5) milk halves. New intramammary infections developed during the dry period also occurred in 5% (n=1) of the milk halves. In the second post-partum study, subclinical mastitis was observed only in 5% (n=1). Healing was achieved in 30% (n = 6) and the new intramammary infections (NIMIs) were 10% (n=2). In the control group, healing was only observed in 5% (n=1) and NIMI were 27.78% (n=5). The application of both treatment approaches, results in reduction in prevalence and manifestation of mastitis, as well as reduction of somatic cells in milk after kidding. The selective treatment method may be preferred, in order to reduce the cost of medications in conducting treatment and prophylaxis programs.

scholarly journals OPERATION STRESS AND ITS INFLUENCE ON THE SYSTEM CHANGES IN CASE OF ABDOMINAL COMBINED TRAUMA (EXPERIMENTAL RESEARCH)

2018 ◽  
Vol 17 (2) ◽  
pp. 31-36
Author(s):  
V. O. Krylyuk ◽  
V. K. Hrodetsʹkyy ◽  
S. O. Sokolʹnyk ◽  
O. V. Fedosyeyeva ◽  
H. Yu. Tsymbalyuk
Keyword(s):  
Superoxide Dismutase ◽  
Abdominal Cavity ◽  
The Body ◽  
Control Group ◽  
Systemic Response ◽  
Antioxidant Protection ◽  
Operational Stress ◽  
Experimental Animals ◽  
Ischemic Reperfusion ◽  
The Right

The aim of the study was to study the possibility of using a hemostatic biological haemostatic to stop bleeding from the parenchymal organs of the abdominal cavity using the surgical method of Damage control. The experiment was performed on laboratory rats that were divided into three groups: a control group, a group where, after injury, the liver wound was sutured with standard sutures and a group where the wound was plastered with a biological hemostatic agent. In order to determine the operational stress, we studied the enzymatic link of antioxidant protection - the level of catalase and superoxide dismutase. To study the indices in all experimental animals, the following tissues were taken: the lower lobe of the right lung, the proportion of the liver where the wounds were applied, the gastrocnemius muscle below the harness and the right kidney. The control points of the study were 1, 3 and 7 days after injury. During working with experimental animals, they adhered to all ethical norms established by international rules. The effect of operational stress on the enzymatic component of antioxidant protection in the tissues of the kidneys, liver, lungs and muscles was studied in the study of the combined trauma of the abdominal cavity complicated by massive bleeding and ischemic-reperfusion syndrome of the extremity. The changes in the indices of superoxide dismutase and catalase were studied. The following result was obtained: tamponing the liver wound with chitosan was a quick and effective means of temporary bleeding stop. At the same time, the duration of the operation was reduced from 19.8 to 12.5 minutes (by 36.8%) (p <0.05). Also, the level of catalase and superoxide dismutase was significantly lower in the group where the chitosan of the liver injury was used in comparison with the study group where standard stitches were applied. The deviations, which are determined in lung, liver, kidney and muscle tissues in RG-1 are evidence of the depth of their lesion and dysfunction, is a favorable factor for the further development of the triggering mechanisms of the systemic response of the body to inflammation and multiple organ failure. In contrast to the RG-2 rapid development of the systemic response was avoided. We can assume that ischemic-reperfusion syndrome is a stimulating factor in the development of systemic changes, although this statement requires further proof. It is also important to further prospective study of this topic in terms of the following questions: what effect has chitosan on liver tissue with prolonged exposure (more than 2 days) and what effect does additional injection of infusion solutions have as an agent for the treatment of hypovolemic shock.

scholarly journals Influence of melatonin on the kidneys of rats with experimental diabetes mellitus type 2

2020 ◽  
Vol 11 (3) ◽  
pp. 384-391
Author(s):  
A. V. Semenko ◽  
Y. V. Murdasov ◽  
S. V. Kirichenko ◽  
V. I. Zhyliuk ◽  
G. A. Ushakovа
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Superoxide Dismutase ◽  
Experimental Studies ◽  
The Body ◽  
Control Group ◽  
Negative Consequences ◽  
Oxidized Products

Diabetes mellitus is characterized by numerous pathological changes in the body. Under conditions of diabetes, hyperglycemic intoxication of the organism rapidly develops, which in turn leads to an increase of oxidative stress with subsequent disturbance of the anatomical and functional integrity of the components of organisms. Today, the search for the substances that would contribute to the multi-vectoral effect on the negative consequences of diabetes is actively being pursued. Melatonin is one of such substances. In this work, we studied the effect of melatonin on oxidative stress markers (oxidized products content, activities of superoxide dismutase and catalase), the concentration of metabolism end products (creatinine and urea), main ions concentration (potassium and chlorine), and protein content (total protein and electropherogram in polyacrylamide gel), enzymatic activity of gamma-glutamyltrasferase in the cytosolic fraction of rat kidneys under condition of type 2 diabetes mellitus (EDM2). Experimental studies were performed on 18 white adult Wistar rats divided into three groups (control, group with EDM2 and group with EDM2, which were treated with melatonin). The increase of concentration of oxidized products, the activity of catalase and gamma-glutamyltrasferase, creatinine, urea, K+ and Cl– and the decrease of concentration of superoxide dismutase in the rats’ kidneys was noted after development of EDM2. The electrophoretic proteinogram of the cytosolic proteins obtained from the rats’ kidneys showed an increase of content of high-molecular-weight and a decrease of low-molecular-weight proteins. Administration of melatonin in a dose of 10 mg/kg of body weight for 7 days after development of EDM2 restored the studied parameters almost to the control group values. Therefore, the influence of melatonin can prevent chronic development of oxidative stress in kidneys under hyperglycemic intoxication, and lead to normalization of kidney function and the restoration of homeostasis.

scholarly journals ANTIOXIDANT PROTECTION SYSTEM DURING CHRONIC INFLAMATION OF THE CONNECTIVE TISSUE IN COWS

2012 ◽  
Vol 10 (3) ◽  
pp. 53-54
Author(s):  
Konstantin Aleksandrovich Nadein
Keyword(s):  
Superoxide Dismutase ◽  
Connective Tissue ◽  
Glutathione Reductase ◽  
Chronic Inflammation ◽  
Ankle Joint ◽  
Protection System ◽  
Control Group ◽  
Clinical Test ◽  
Antioxidant Protection ◽  
Antioxidant Protection System

As clinical test material we studied the blood serum taken from clinically healthy animals (the control group) and animals with chronic inflammation of the connective tissue in an ankle joint (the experimental group). To study the antioxidant protection system enzyme component condition in cows we examined the following: catalase and glutathione peroxidase activity (as a substrate we used hydrogen dioxide and glutathione reductase), ferroxidase activity of ceruloplasmin, superoxide dismutase activity. A spectrocolorimeter «Specol 210» was used to determine the solutions absorbancy. The results shows the breakdown of membrane-bounded enzymes activity (such as glutathione reductase and superoxide dismutase). These enzymes characterize the antioxidant protection system condition. It is essential to examine the antioxidant protection system condition in animals with chronic inflammation of the connective tissue in an ankle joint to enrich our knowledge about the mechanism of metabolic disorder at this type of pathology, as well as to treat and correct possible complications.

scholarly journals Antioxidant protection enzyme activity in the blood serum and large intestinal mucosa of rats with prolonged gastric hypochlorhydria and given multiprobiotics

2019 ◽  
Vol 10 (4) ◽  
pp. 438-444
Author(s):  
S. V. Pylypenko ◽  
A. A. Koval
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Glutathione Reductase ◽  
Catalase Activity ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Control Group ◽  
Antioxidant Protection ◽  
Colon Mucosa

The activity of antioxidant protection enzymes in the blood serum and colon mucosa in rats was studied under the conditions of 28-days administration of omeprazole on its own and omeprazole together with multiprobiotics "Symbiter" and "Apibact". Physiological and biochemical study methods were applied. It was found that after omeprazole administration, the activity of superoxide dismutase in the blood serum decreased, and the activity of catalase increased compared to the control. With the co-administration of omeprazole and multiprobiotics, the activity of superoxide dismutase increased compared to the group of rats that received omeprazole only during the same time, but remained less compared to the control group. The content of reduced glutathione in the blood serum of rats after administration of omeprazole decreased, the activity of glutathione peroxidase and glutathione transferase increased, and the activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics, the serum RG content was at the control level, the activity of glutathione reductase exceeded the control values. The activity of glutathione reductase decreased compared to the group receiving omeprazole only. The activity of glutathione reductase increased and did not differ from the control values. In the colon mucosa, superoxide dismutase and catalase activity decreased compared to control. With the combined administration of omeprazole and multiprobiotics, superoxide dismutase and catalase activity increased and even exceeded the control values. With the administration of omeprazole, the reduced glutathione content in the colon mucosa was lower than that in the control. The activity of glutathione peroxidase increased and glutathione transferase and activity of glutathione reductase decreased compared to the control. With co-administration of omeprazole and multiprobiotics to rats, the reduced glutathione content increased compared to the group of rats administered omeprazole only, and even exceeded that in the control.

scholarly journals Application of bacillus megaterium for subclinical mastitis in cows

2021 ◽  
pp. 32-34
Author(s):  
Yaroslav Tytukh ◽  
Yurii Musiienko ◽  
Natalia Grebenik
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Bacillus Megaterium ◽  
Somatic Cells ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Control Group ◽  
Opportunistic Pathogens ◽  
Opportunistic Pathogenic ◽  
Positive Effect

The problem of treatment of subclinical mastitis often arises after calving, especially in cows of the first lactation. The paper presents the results of the therapeutic effect of Bacillus megaterium in subclinical mastitis in cows. The aim of the research. To determine the effect of Bacillus megaterium on the microflora of the gastrointestinal tract and colonies of microorganisms isolated from subclinical mastitis in cows. Materials and methods. The research was conducted in a dairy farm growing Holstein. Cows with subclinical mastitis of the experimental groups were given concentrated feed with the addition of Bacillus megaterium (1 × 109 CFU/g) at a dose of 15–35 g per animal. The control group of cows was given the usual diet for dairy cows. The experiment lasted 30 days. Results. The use of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal had a pronounced effect on the microflora of the gastrointestinal tract of cows. The amount of Lactobacillus sp. was 67 % higher and Bifidobacterium 58 % higher than the control. In addition, the level of opportunistic pathogens on Escherichia coli decreased by 45 %, Clostridium by 27 %, Enterobacteriaceae and Staphylococcus by 75 %, and Candida by 80 % compared to controls. The amount of microflora in milk decreased by 40.2 % and the number of somatic cells by 87.9 %. Conclusions. The therapeutic efficacy of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal for 30 days in subclinical mastitis has been proven. After treatment, the amount of microflora in cow's milk decreased by 40.2 % and the number of somatic cells by 87.9 %. A positive effect on the microflora of the gastrointestinal tract of cows, where the number of Lactobacillus sp. increased by 67 % and Bifidobacterium by 58 %. The level of opportunistic pathogenic microflora decreased by Escherichia coli – by 45 %, Clostridium – by 27 %, Enterobacteriaceae and Staphylococcus – by 75 %, Candida – by 80 %, compared to the control.

scholarly journals Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes

2021 ◽  
Vol 11 (3) ◽  
pp. 12-17
Author(s):  
A. V. Elikov
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Former Athletes ◽  
Oxidative Balance

Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.

scholarly journals Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

2017 ◽  
Vol 7 (4) ◽  
pp. 589-596 ◽  
Author(s):  
B. Gutyj ◽  
V. Stybel ◽  
L. Darmohray ◽  
Y. Lavryshyn ◽  
I. Turko ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Enzyme Level ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Peroxidation Of Lipids ◽  
Antioxidant Protection ◽  
Diene Conjugates ◽  
Young Cattle ◽  
Antioxidant Protection System

<p>The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase - by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium - by 18%, vitamin A - by 23%, vitamin E - by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.</p>

scholarly journals The role of linoleic acid in oxidative damage of hemoglobin due to cytomegelavirus infection during pregnancy

2019 ◽  
Vol 18 (3) ◽  
pp. 15-20
Author(s):  
I. A. Andrievskaya ◽  
N. A. Ishutina
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Linoleic Acid ◽  
Glutathione Peroxidase ◽  
Oxidative Damage ◽  
Pregnant Women ◽  
Cytomegalovirus Infection ◽  
First Trimester ◽  
Oxidative Modification ◽  
Control Group

The goal is to study the mechanisms of linoleic acid-dependent oxidative damage to hemoglobin in blood erythrocytes of pregnant women with cytomegalovirus (CMV) infection during the first trimester.Materials and methods. The study included 55 CMV-seropositive pregnant women at 8–11 weeks pregnant, aged 24,7 ± 0,18 years. 20 women had exacerbated cytomegalovirus infection and in 35 women a latent course of the disease was observed. The control group consisted of 20 CMV-seronegative pregnant women, comparable in age and gestational age with the group of pregnant women with cytomegalovirus infection. The levels of linoleic acid, superoxide dismutase, glutathione peroxidase and methemoglobin in the blood were studied by the spectrophotometry. Glutathione was determined by histochemical methods, the total amount of hemoglobin was measured using the automatic hematological analyzer, and hydrogen peroxide level was calculated by enzyme immunoassay.Results. In cytomegalovirus infection, an increase in the indices of exogenous hydrogen peroxide and linoleic acid in blood erythrocytes of pregnant women is observed. During the period of acute disease in the first trimester of pregnancy it causes oxidative modification and a decrease in the level of not only superoxide dismutase, glutathione peroxidase and glutathione, the processes involved in the redox regulation of thiol-disulfide exchange of erythrocytes, but also hemoglobin with formation of large amount of methemoglobin, which contributes to reduction of oxygen metabolism and development of hemic hypoxia. In the latent course of the disease, the oxidative damage to hemoglobin in the erythrocytes of pregnant women is mitigated by the increase in the activity of redox enzymes, which maintains oxygen homeostasis at the level necessary for the development of pregnancy. 

Sign in / Sign up

Export Citation Format

Share Document