Application of bacillus megaterium for subclinical mastitis in cows

The problem of treatment of subclinical mastitis often arises after calving, especially in cows of the first lactation. The paper presents the results of the therapeutic effect of Bacillus megaterium in subclinical mastitis in cows. The aim of the research. To determine the effect of Bacillus megaterium on the microflora of the gastrointestinal tract and colonies of microorganisms isolated from subclinical mastitis in cows. Materials and methods. The research was conducted in a dairy farm growing Holstein. Cows with subclinical mastitis of the experimental groups were given concentrated feed with the addition of Bacillus megaterium (1 × 109 CFU/g) at a dose of 15–35 g per animal. The control group of cows was given the usual diet for dairy cows. The experiment lasted 30 days. Results. The use of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal had a pronounced effect on the microflora of the gastrointestinal tract of cows. The amount of Lactobacillus sp. was 67 % higher and Bifidobacterium 58 % higher than the control. In addition, the level of opportunistic pathogens on Escherichia coli decreased by 45 %, Clostridium by 27 %, Enterobacteriaceae and Staphylococcus by 75 %, and Candida by 80 % compared to controls. The amount of microflora in milk decreased by 40.2 % and the number of somatic cells by 87.9 %. Conclusions. The therapeutic efficacy of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal for 30 days in subclinical mastitis has been proven. After treatment, the amount of microflora in cow's milk decreased by 40.2 % and the number of somatic cells by 87.9 %. A positive effect on the microflora of the gastrointestinal tract of cows, where the number of Lactobacillus sp. increased by 67 % and Bifidobacterium by 58 %. The level of opportunistic pathogenic microflora decreased by Escherichia coli – by 45 %, Clostridium – by 27 %, Enterobacteriaceae and Staphylococcus – by 75 %, Candida – by 80 %, compared to the control.

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Use of probiotics for formation of microflora of gastrointestinal tract of calves

EUREKA Health Sciences ◽

10.21303/2504-5679.2021.001951 ◽

2021 ◽

pp. 94-100

Author(s):

Oksana Shkromada ◽

Yuliia Dudchenko ◽

Yana Udovenko

Keyword(s):

Gastrointestinal Tract ◽

Bacillus Megaterium ◽

Biochemical Parameters ◽

Bacillus Pumilus ◽

Bacillus Coagulans ◽

Control Group ◽

Opportunistic Pathogens ◽

Bacillus Mucilaginosus ◽

Probiotic Strains ◽

Experimental Group

The results of the use of probiotic strains of microorganisms of the Bacillus family for the correction and formation of the microflora of the gastrointestinal tract and the impact on metabolism in calves are presented. The aim of the study. To analyze the effect of probiotics on the microflora of the gastrointestinal tract in calves and biochemical parameters of blood in calves up to one month. Materials and methods. The research was conducted during 2020 in the conditions of Ukrainian farms for cattle breeding. Five experimental groups of five one-week-old calves were formed in each and one control group. Calves were kept separately in the same conditions on the same diet, but with feeding together with colostrum substitute probiotics of five grams per animal: Bacillus amyloliquefaciense, Bacillus mucilaginosus, Bacillus coagulans, Bacillus megaterium, Bacillus pumilus. The strains are deposited and produced by “Kronos Agro” Ukraine. Results. It was found that as a result of studies when feeding calves B. coagulans, B. pumilus and B. mucilaginosus the number of Lactobacillus sp. was 80 % higher than in the control group. The level of opportunistic pathogens in the experimental group with B. coagulans had minimal values. Animals in the group where B. mucilaginosus was given had a higher amount of Candida - up to 300 CFU/g and Enterobacteriaceae – 200 CFU/g; which is 50 % less compared to control groups, but more than in the experiment with B. coagulans. According to the results of biochemical examination of blood serum in calves, the absence of toxic effects of probiotic strains: Bacillus amyloliquefaciense, Bacillus mucilaginosus, Bacillus coagulans, Bacillus megaterium, Bacillus pumilus on the internal organs of animals was established. Conclusions. It was found that the maximum positive effect on the microflora of the gastrointestinal tract of calves up to 30 days of age had B. coagulans (1×109) when fed at a dose of 5 g per animal. The amount of Lactobacillus sp. was the maximum and reached 800 CFU/g, which is 80 % more than in the control group. At the same time, the level of opportunistic pathogens in the experimental group with B. coagulans had minimal indicators and was: Clostridium by 20 %, Escherichia coli – by 70 %, Enterobacteriaceae, Staphylococcus and Candida – 100 % less than the control. In the study of biochemical parameters, it was found that the activity of enzymes, protein and glucose levels in the serum of experimental animals fed with B. coagulans were within the physiological norm, indicating a normal metabolic process and no toxic effects.

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Dietary fermented rapeseed or/and soybean meal additives on performance and intestinal health of piglets

Scientific Reports ◽

10.1038/s41598-021-96117-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Anna Czech ◽

Eugeniusz Ryszard Grela ◽

Martyna Kiesz

Keyword(s):

Gastrointestinal Tract ◽

Gut Microbiota ◽

Soybean Meal ◽

Rapeseed Meal ◽

Nutrient Digestibility ◽

Control Group ◽

Feed Conversion ◽

Weaned Piglets ◽

Feed Conversion Rate ◽

Positive Effect

AbstractThe aim of the study was to assess the effect of fermented dried soybean (FSBM) and/or fermented rapeseed meal (FRSM) in diets for weaned piglets on production results, nutrient digestibility, gastrointestinal tract histology, and the composition of the gut microbiota. Piglets in the control group received standard diets with soybean meal. Animals in all experimental groups received diets in which a portion of the soybean meal was replaced: in group FR—8% FRSM; in group FR/FS—6% FRSM and 2% FSBM; in group FS/FR—2% FRSM and 6% FSBM and in group FS—8% FSBM. The use of 8% FRSM or 6% FRSM and 2% FSBM in the piglet diets had a positive effect on average daily gains. Piglets from the FR and FR/FS groups had the highest feed conversion rate. Group FS/FR and FS piglets had significantly lower mortality and lower incidence of diarrhoea. Piglets fed a diet with the fermented components, in particular with 8% FRSM or 6% FRSM and 2% FSBM, exhibited a positive effect on the microbiological composition and histology of intestines, which resulted in improved nutrient digestibility coefficients (ATTD and AID).

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MEASURES FOR IMPROVING THE MICROBIOLOGICAL SAFETY OF COW MILK

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.201901007 ◽

2019 ◽

Vol 1 (1) ◽

pp. 44-49

Author(s):

G. A. Larionov ◽

◽

O. Yu. Checheneshkina ◽

N. V. Mardaryeva ◽

N. V. Sсhiptsova ◽

...

Keyword(s):

Somatic Cells ◽

Research Work ◽

Dairy Farm ◽

Cow Milk ◽

Control Group ◽

Anaerobic Microorganisms ◽

Special Means ◽

Before And After ◽

Processing Enterprise

Safety of milk on microbiological indicators is an important condition for acceptance on the milk processing enterprise. High microbial contamination of milk and increased content of somatic cells in milk are often the cause of discrepancy to modern requirements of national and international regulatory documents. The development and implementation of measures aimed at improving the condition of the udder of cows, the use of modern means of processing is one of the most important tasks to improve the sanitary and hygienic conditions of milk production. Prevention of mastitis is one of the effective ways to improve the safety and quality of cows' milk. It is known that the most affordable way to prevent mastitis is strict hygiene during milking. In our article the results of research work received in the conditions of a dairy farm are presented. Researches on use of modern domestic means of processing of an udder before and after milking are carried out. For this, two experimental and one control groups were created. In summer and autumn, the udder of cows in the experimental groups were treated with special detergents and disinfectants. In the control group, the udders were not treated with special means. Udder hygiene was maintained by washing the nipples with warm water, as is customary in the household. It was established that the treatment of the udder of cows with disinfectants decreased the number of mesophilic aerobic and facultatively anaerobic microorganisms and somatic cells in the milk of cows.

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Effects of Using an Alternative Bedding Composition on the Levels of Indicator Microorganisms and Mammary Health in Dairy Farm Conditions

Agriculture ◽

10.3390/agriculture10060245 ◽

2020 ◽

Vol 10 (6) ◽

pp. 245

Author(s):

František Zigo ◽

Nad’a Sasáková ◽

Gabriela Gregová ◽

Jana Výrostková ◽

Silvia Ondrašovičová

Keyword(s):

Dairy Cows ◽

Dairy Farm ◽

Subclinical Mastitis ◽

Mammary Glands ◽

Fecal Coliforms ◽

Time Intervals ◽

Indicator Microorganisms ◽

Actual Observation ◽

Positive Effect ◽

Observation Period

The aim of this study was to compare an improved bedding composition with conventional straw bedding under farm conditions, regarding its effects on the influence of indicator microorganisms on the hygiene levels of cubicle floors and the occurrence of mastitis in dairy cows. Dairy cows were housed in newly built stalls divided into two parts, each with four subsections, and bedded cubicles arranged in three rows. Five stall subsections from each 9-bedded cubicle were selected for study, and 30 dairy cows were monitored according to the time intervals of bedding treatment for cubicles. In the first subsection (control), the cows were housed in bedded cubicles layered with straw up to a height of 20 cm. Sections 2–5 had alternative bedding (AB) as follows: fresh AB, AB 1 month old, AB 2 months old, and AB 3 months old, which were bedded one day before (fresh) and 1–3 months before the actual observation period, respectively. The alternative bedding per one cubicle consisted of ground limestone (100 kg), water (80 L), recycled manure solids (RMS; 15 kg), and straw (25 kg). After laying, the bedding was treated with a concrete selector to provide strength and sufficient resistance. A total of 180 bedding and 600 quarter milk samples were taken simultaneously from all five monitored subsections for microbiological determination. Comparing classical straw bedding with the alternate bedding showed a stabilizing effect by keeping the bedding thickness up to the floor barrier level, which had a beneficial effect by reducing the level of fecal contamination in the rear of the cubicle. Fecal coliforms and fecal streptococci were found to be reduced in one-day-old bedding as well as after the first, second, and third months. By evaluating the health status of the mammary glands, a positive effect was noted in reducing the occurrence of subclinical mastitis, which was reflected in a reduced number of infected quarters in the group of cows housed in cubicles for three months after use of improved bedding.

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Use of a Nonmedicated Dietary Supplement Correlates with Increased Prevalence of Streptomycin-Sulfa-Tetracycline-Resistant Escherichia coli on a Dairy Farm

Applied and Environmental Microbiology ◽

10.1128/aem.02584-05 ◽

2006 ◽

Vol 72 (7) ◽

pp. 4583-4588 ◽

Cited By ~ 44

Author(s):

Artashes R. Khachatryan ◽

Thomas E. Besser ◽

Dale D. Hancock ◽

Douglas R. Call

Keyword(s):

Escherichia Coli ◽

Dietary Supplement ◽

Dairy Farm ◽

Washington State ◽

Growth Characteristics ◽

Control Group ◽

Antibiotic Resistant ◽

E Coli ◽

Dry Milk ◽

Cell Densities

ABSTRACT We examined how a dietary supplement affects the prevalence of antibiotic-resistant Escherichia coli on a dairy farm in Washington State. Between 2001 and 2004 the prevalence of fecal E. coli strains resistant to streptomycin, sulfadiazine, and tetracycline (SSuT strains) declined from 59.2% to 26.1% in the calf population. In 2003 the dairy discontinued use of a dietary supplement, and we hypothesized that the decline in prevalence of SSuT strains was related to this change in management. To test this we established three treatments in which calves received no supplement, the dietary supplement with oxytetracycline, or the dietary supplement without oxytetracycline. Calves receiving either dietary supplement had a significantly higher prevalence of SSuT E. coli than the no-supplement control group (≈37% versus 20%, respectively; P = 0.03). Importantly, there was no evidence that oxytetracycline contributed to an increased prevalence of fecal SSuT E. coli. We compared the growth characteristics of SSuT and non-SSuT E. coli in LB broth enriched with either the complete dietary supplement or its individual constituents. Both the complete dietary supplement and its vitamin D component supported a significantly higher cell density of SSuT strains (P = 0.003 and P = 0.001, respectively). The dry milk and vitamin A components of the dietary supplement did not support different cell densities. These results were consistent with selection and maintenance of SSuT E. coli due to environmental components independent of antibiotic selection.

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STUDY ON THE EFFECTIVENESS OF INTRACISTERNAL ANTIBIOTIC TREATMENT FOR THE CONTROL OF MASTITIS IN GOATS

BULGARIAN JOURNAL OF VETERINARY MEDICINE ◽

10.15547/tjs.2020.01.007 ◽

2020 ◽

Vol 23 (1) ◽

pp. 37-40

Author(s):

K. Hristov ◽

R. Pepovich ◽

B. Nikolov

Keyword(s):

Post Partum ◽

Somatic Cells ◽

Treatment Method ◽

Subclinical Mastitis ◽

Control Group ◽

Dry Period ◽

Selective Treatment ◽

Intramammary Infections ◽

Selective Approach ◽

The Cost

The aim of this study is to investigate the healing and prophylactic effect of drying-off antibiotic therapy using the selective and non-selective approach of administration of the antibiotics. First experimental group at the start of the dry period were treated non-selectively (all halves) intra-cisternally with antibiotics. In the second group, we only treated those halves that were diagnosed with subclinical mastitis. The control group of 9 goats was not treated. The number of somatic cells in treated groups after birth decreased from 2586 x 10³/ mL to 560 x 10³/mL in the first group and from 1978 x 10³/mL to 526 x 10³/mL in the second. Post partum, 10% of the halves were found to have subclinical mastitis. A healing effect was achieved in 25% (n=5) milk halves. New intramammary infections developed during the dry period also occurred in 5% (n=1) of the milk halves. In the second post-partum study, subclinical mastitis was observed only in 5% (n=1). Healing was achieved in 30% (n = 6) and the new intramammary infections (NIMIs) were 10% (n=2). In the control group, healing was only observed in 5% (n=1) and NIMI were 27.78% (n=5). The application of both treatment approaches, results in reduction in prevalence and manifestation of mastitis, as well as reduction of somatic cells in milk after kidding. The selective treatment method may be preferred, in order to reduce the cost of medications in conducting treatment and prophylaxis programs.

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Influence of liposomal drug on the intensity of proteins oxide modification processes in subclinical mastitis of cows

The Animal Biology ◽

10.15407/animbiol23.02.003 ◽

2021 ◽

Vol 23 (2) ◽

pp. 3-7

Author(s):

V. A. Chepurna ◽

◽

T. M. Suprovych ◽

O. I. Vishchur ◽

V. P. Mizik ◽

...

Keyword(s):

Superoxide Dismutase ◽

Somatic Cells ◽

Experimental Studies ◽

Study Drug ◽

Subclinical Mastitis ◽

Oxidative Modification ◽

Control Group ◽

Antioxidant Protection ◽

Liposomal Drug ◽

Intracisternal Injection

The article contains the experimental studies of the liposomal drug based on plant raw materials ― hypericum (Hypericum perforatum L.) effect on the intensity of oxidative modification of proteins (OMP) in the blood and milk of cows with subclinical mastitis. Studies have shown that cows with signs of subclinical form of mastitis in the serum have an increase in the content of aldehyde-derived OMP370 and ketone-derived OMP430, respectively, 1.3 and 1.2 times relative to similar indicators in healthy animals. In the milk of sick cows, the content of derivatives OMP370 and OMP430 was1.99 and 2.29 times higher, respectively, than in animals of the control group. At the beginning of the study sick cows’ milk was recorded a significantly low value of the activity of the enzymatic link of antioxidant protection ― superoxide dismutase. At the same time, a 2.6-fold (P<0.001) increase in the number of somatic cells was noted compared to their number in the milk of clinically healthy cows. Intracisternal injection of liposomal drug to cows caused a decrease in the intensity of oxidative processes. In the blood of sick cows the content of aldehyde derivatives OMP370 on the 9th day of the experiment was 23.1% (P<0.05) less than before the drug, and in milk the content of OMP370 decreased by 61.8% (P<0.01). Similar changes were observed with respect to the level of ketone derivatives. In particular, on the 9th day of the experiment, the content of OMP430 decreased by 11.7% (P<0.05) compared with its value in the blood of sick animals before the introduction of the study drug, and in milk it decreased by 64.2% (P<0.01). During the treatment on the 9th day of the experiment, the number of somatic cells in milk decreased by 41.8% (P<0.01). In the course of treatment on the 3rd and 9th day there was a tendency to increase superoxide dismutase activity in the milk of sick cows compared with the beginning of the experiment. Thus, intracisternal injection of liposomal drug to cows with subclinical mastitis leads to a decrease in aldehyde and ketone derivatives of proteins oxidative modification in serum and milk. At the same time, an increase in the activity of the enzymatic link of antioxidant protection and a decrease in the number of somatic cells in the milk of cows were recorded.

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Effects of Suckling on the Udder Health of Foster Cows

Ruminants ◽

10.3390/ruminants1020008 ◽

2021 ◽

Vol 1 (2) ◽

pp. 100-117

Author(s):

Katharina Köllmann ◽

Yanchao Zhang ◽

Nicole Wente ◽

Anneke Lücken ◽

Stefanie Leimbach ◽

...

Keyword(s):

Dairy Farm ◽

Visual Assessment ◽

Subclinical Mastitis ◽

Pathogen Transmission ◽

Control Group ◽

Suckling Period ◽

Intramammary Infections ◽

Udder Health ◽

The Status ◽

Rearing Methods

In a foster cow system, one or more calves are reared by a foster cow. With regard to the growing rejection towards the early separation of cow and calf after calving, such alternative rearing methods with prolonged cow–calf contact are coming more into focus. Nevertheless, there is a lack of recent studies investigating the effects of suckling multiple calves on the udder health of a foster cow. The present study aimed to initially describe the status and development of the udder condition and the microbial colonisation during the foster period. Thereby, the objectives were to determine whether suckling multiple calves was associated with an increase in intramammary infections (IMI) and a deterioration of udder constitution. A total of 99 Holstein Friesian foster cows from a large organic dairy farm in eastern Germany were included in this pilot study. Each foster cow was suckled by 3–4 calves and not additionally milked. Quarter milk samples were taken for cytomicrobiological examination at the beginning and end of the approximately 3.5 months suckling period. At the same time, the udders of 91 of these foster cows were clinically examined by visual assessment and manual palpation. In both the first and second examination (E1 and E2, respectively), Corynebacterium ssp. and non-aureus staphylococci (NaS) constituted the most frequently detected pathogen species in the 385 examined udder quarters. Classifying the quarters into mastitis categories was significantly different between the two examinations (p = 0.001). The number of quarters considered as healthy increased from E1 to E2 by 8.5%. At the same time, the number of quarters with mastitis decreased by 2.6%, the number of quarters with non-specific mastitis by 11.4% and the number of quarters with a latent infection increased by 5.4%. In total, 69.4% of the 121 infected udder quarters in E1 were bacteriologically cured in E2. A cytological cure was achieved in 28.1% of the 385 quarters. Both the bacteriological and cytological cure rate were spontaneous. A new infection occurred in 23.1% of the 385 examined udder quarters and was mainly attributable to infections with Corynebacterium spp. and NaS. During the suckling period, mainly new infections occurred and amounted to 73% in relation to presumed persistent infections (18.9%). The new infections were mainly attributable to infections with Corynebacterium spp. and NaS. Pasteurella spp. were also found in high percentages. A total of 10.1% of the quarters showed an increase in somatic cell count (SCC) above the threshold of 100,000 cells/mL from E1 to E2, indicating a new subclinical mastitis. Regarding udder condition, significant changes during the foster period were an increase in udder skin and teat lesions, a decrease in udder oedema, an increased amount of udder quarters with a consistency considered as normal and an increase in the number of udders showing no sign of udder cleft dermatitis. Moreover, teat length, circumference, and diameter increased significantly (p < 0.05). In conclusion, there is a possibility that suckling multiple calves improves udder health in terms of mastitis but leads to an increased pathogen transmission at the same time, resulting in an increase in latent infections. Additionally, pathogens as Pasteurella spp., which are usually located in the upper respiratory system, could be detected in the mammary gland. The changes concerning the udder condition were manifold. However, it has not yet been conclusively clarified whether all these changes are exclusively due to calf suckling. Further investigations with a non-suckled control group are required.

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Control of subclinical bovine mastitis

British Homeopathic Journal ◽

10.1016/s0007-0785(95)80033-6 ◽

1995 ◽

Vol 84 (02) ◽

pp. 67-70 ◽

Cited By ~ 12

Author(s):

O. Reyes ◽

G. Guajardo ◽

R. Searcy

Keyword(s):

Milk Production ◽

Bovine Mastitis ◽

Dairy Farm ◽

Subclinical Mastitis ◽

Treated Group ◽

Control Group ◽

California Mastitis Test ◽

Animal Populations ◽

The Difference ◽

Random Method

AbstractA clinical trial was conducted on a dairy farm. 26 animals were divided into 2 groups of 13 each, pairing on clinical and subclinical mastitis status, milk production and number of lactations, assigning both animals and treatments by a systematic random method. One group received homoeopathic treatment, the other placebo. Monthly milk production was carefully recorded for each animal, as were the results of the California Mastitis Test (CMT) performed on each quarter of the udder. The proportion of affected quarters according to CMT was 32% in the treated group, and 68% in the control group. The odds ratio of the difference shows that animals receiving placebo presented 4.5 (1.78–11.73) times more subclinical mastitis than those under homoeopathic treatment (p<0.05). Average milk production in the treated group did not differ significantly from that of the control group (p>0.05). The study confirms previous observations of the benefit the homoeopathic method can provide in disease control in animal populations.

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Pulsed-Field Gel Electrophoresis Used for Typing of Extended-Spectrum-β-Lactamases- Producing Escherichia coli Isolated from Infant ҆S Respiratory and Digestive System

Macedonian Veterinary Review ◽

10.2478/macvetrev-2018-0016 ◽

2018 ◽

Vol 41 (2) ◽

pp. 133-141 ◽

Cited By ~ 1

Author(s):

Gorica Popova ◽

Dean Jankuloski ◽

Benjamin Felix ◽

Katerina Boskovska ◽

Biljana Stojanovska-Dimzovska ◽

...

Keyword(s):

Escherichia Coli ◽

Gastrointestinal Tract ◽

Respiratory Tract ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Control Group ◽

Study Group ◽

E Coli ◽

Hospital Acquired ◽

Susceptibility Patterns

Abstract Escherichia coli infections are becoming increasingly difficult to treat because of emerging antimicrobial resistance, mostly to expanded-spectrum cephalosporins, due to the production of extended-spectrum β-lactamases (ESBLs).Despite extensive studies of ESBL- producing E.coli in adult patients, there is a lack of information about the epidemiology and spread of ESBL organisms in pediatric population. The aim of this study was to examine the gastrointestinal tract as an endogenous reservoir for the respiratory tract colonization with ESBL- E. coli in children, hospitalized because of the severity of the respiratory illness. The study group consists of 40 children with ESBL-producing E. coli strains isolated from the sputum and from the rectal samples. A control group of 15 E. coli isolated from rectal swabs of healthy children were included in the analysis. The comparison of the strains was done by using antimicrobial susceptibility patterns of the stains, and pulsed field gel electrophoresis was performed for molecular typing, using XbaI digestion. 90% of the compared pairs of strains in the study group were with identical antimicrobial susceptibility patterns and indistinguishable in 79.2% by the obtained PFGE – profiles.33.3% (5/15) of confirmed E. coli strains from the control group were found to be ESBL – producers. Resulting band profiles of all isolates demonstrated presence of 12 pulsotypes, with 100% similarity within the pulsotypes. Although, some isolates obtained from different patients were genetically indistinguishable, these strains were not hospital acquired, as none of the patients satisfied the criteria for hospital acquired pneumonia, and there was a lack of an obvious transmission chain. All ESBL –E. coli isolated from sputum in clinical cases were obtained from patients under the age of one. According to the resistance profile of the compared pairs and the PFGE comparison of all isolates, it can be concluded that the gastrointestinal tract is the main reservoir of ESBL-E. coli. Small age in infants is a risk factor for translocation of bacteria, enabling the colonization of the respiratory tract.

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