scholarly journals CULTIVATION OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS IN COLLAGEN AND FIBRIN HYDROGELS: EFFECTS ON CELL VIABILITY AND PROLIFERATION

2014 ◽  
Vol 7 (5) ◽  
pp. 50-54 ◽  
Author(s):  
R. G. Vasyliev ◽  
Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S223 ◽  
Author(s):  
V Grytsyk ◽  
A Rodnichenko ◽  
O Gubar ◽  
O Rybachuk ◽  
A Zlatska ◽  
...  

Author(s):  
Rajaventhan Srirajaskanthan ◽  
Martyn E. Caplin ◽  
Humphrey Hodgson

Neuroendocrine tumours (NETs) are derived from cells of the diffuse neuroendocrine system, which are present in organs throughout the body. Originally, Pearse proposed that tumours develop from migration of cells from the neural crest; however, it is now thought that the tumour cells are derived from multipotent stem cells (1). The term ‘karzinoide’ (meaning carcinoma like) was initially introduced by Siegfried Oberndorfer in 1907 (2). The term carcinoid tumour has historically been used; however, with advances in the understanding of the tumour biology, and the recent WHO classification, the term NET or endocrine tumour is considered more appropriate, and more details are given in the historical introduction in Chapter 6.1.


2020 ◽  
Vol 522 (4) ◽  
pp. 819-825
Author(s):  
Kai Zhang ◽  
Xiaozhen Cui ◽  
Bochi Zhang ◽  
Xianyi Song ◽  
Qiang Liu ◽  
...  

2017 ◽  
Vol 39 (3) ◽  
pp. 171-180 ◽  
Author(s):  
R G Vasyliev ◽  
A E Rodnichenko ◽  
O S Gubar ◽  
A V Zlatska ◽  
I M Gordiienko ◽  
...  

Aim: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. Materials and Methods: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis. Their clonogenic potential, ability to self-renewal and directed multilineage differentiation were also investigated. Results: Cell cultures were obtained from explants of adult human hair follicles. Resulted cells according to morphological, phenotypic and functional criteria satisfied the definition of neural crest-derived multipotent stem cells. They had the phenotype Sox2+Sox10+Nestin+CD73+CD90+CD105+CD140a+CD 140b+CD146+CD166+CD271+CD349+ CD34-CD45-CD56-HLA-DR-, showed high clonogenic potential, ability to self-renewal and directed differentiation into the main derivatives of the neural crest: neurons, Schwann cells, adipocytes and osteoblasts. Conclusion: The possibility of a large-scale expansion of adult neural crest-derived multipotent stem cells up to 40–200·106 cells from minimal number of hair follicles with retention of their phenotype and functional properties are the significant step towards their translation into the clinical practice.


2018 ◽  
Vol 28 (1) ◽  
pp. 059-063
Author(s):  
Yuri V. Chepurnyi ◽  
◽  
T. V. Kustrjo ◽  
Alina V. Korsak ◽  
Volodymyr V. Likhodievskyi ◽  
...  

2014 ◽  
Vol 30 (6) ◽  
pp. 469-476 ◽  
Author(s):  
R. G. Vasyliev ◽  
A. E. Rodnichenko ◽  
D. A. Zubov ◽  
S. Y. Rymar ◽  
O. S. Gubar ◽  
...  

Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S237
Author(s):  
R Vasyliev ◽  
O Lysenko ◽  
V Grytsyk ◽  
V Shevchun ◽  
A Rodnichenko ◽  
...  

2012 ◽  
Vol 302 (9) ◽  
pp. G958-G965 ◽  
Author(s):  
Laren Becker ◽  
Subhash Kulkarni ◽  
Gunjan Tiwari ◽  
Maria-Adelaide Micci ◽  
Pankaj Jay Pasricha

Enteric neural stem cells (ENSCs) are a population of neural crest-derived multipotent stem cells present in postnatal gut that may play an important role in regeneration of the enteric nervous system. In most studies, these cells have been isolated from the layer of the gut containing the myenteric plexus. However, a recent report demonstrated that neurosphere-like bodies (NLBs) containing ENSCs could be isolated from mucosal biopsy specimens from children, suggesting that ENSCs are present in multiple layers of the gut. The aim of our study was to assess whether NLBs isolated from layers of gut containing either myenteric or submucosal plexus are equivalent. We divided the mouse small intestine into two layers, one containing myenteric plexus and the other submucosal plexus, and assessed for NLB formation. Differences in NLB density, proliferation, apoptosis, neural crest origin, and phenotype were investigated. NLBs isolated from the myenteric plexus layer were present at a higher density and demonstrated greater proliferation, lower apoptosis, and higher expression of nestin, p75, Sox10, and Ret than those from submucosal plexus. Additionally, they contained a higher percentage of neural crest-derived cells (99.4 ± 1.5 vs. 0.7 ± 1.19% of Wnt1-cre:tdTomato cells; P < 0.0001) and produced more neurons and glial cells than those from submucosal plexus. NLBs from the submucosal plexus layer expressed higher CD34 and produced more smooth muscle-like cells. NLBs from the myenteric plexus layer contain more neural crest-derived ENSCs while those from submucosal plexus appear more heterogeneous, likely containing a population of mesenchymal stem cells.


2015 ◽  
Vol 47 (1) ◽  
pp. 80-83 ◽  
Author(s):  
R. G. Vasyliev ◽  
A. E. Rodnichenko ◽  
S. N. Shamalo ◽  
A. S. Demidchouk ◽  
I. F. Labunets ◽  
...  

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