Effect of Cryohemolyzed Human Cord Blood-Derived Low Molecular Fraction on Recovery of Morphological Properties and Metabolism of ATP and 2,3-DPG of Human Donor Blood Erythrocytes Under Hypothermic Storage

Abstract At different developmental stages, candidate human hematopoietic stem cells (HSCs) are present within the CD34+ CD38− population. By means of xenotransplantation, such CD34+CD38− cells were recently shown to engraft the hematopoietic system of fetal sheep and nonobese diabetic severe combined immunodeficient adult mice. Here it is demonstrated that, after their injection into murine blastocysts, human cord blood (CB)–derived CD34+and CD34+ CD38− cells repopulate the hematopoietic tissues of nonimmunocompromised murine embryos and that human donor contribution can persist to adulthood. It is further observed that human hematopoietic progenitor cells are present in murine hematopoietic tissues of midgestational chimeric embryos and that progeny of the injected human HSCs activate erythroid-specific gene expression. Thus, the early murine embryo provides a suitable environment for the survival and differentiation of human CB CD34+ CD38− cells.

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Influence Of Low-Molecular Fraction (Up To 5 Kda) Of Human Cord Blood On Growth Properties Of Culture Of Mesenchymal Stromal Cells Subjected To Cryopreservation

Problems of Cryobiology and Cryomedicine ◽

10.15407/cryo28.02.131 ◽

2018 ◽

Vol 28 (2) ◽

pp. 131-138

Author(s):

Oleksandr K. Gulevsky ◽

◽

Aleksey A. Lavrik ◽

Anna V. Trifonova ◽

Vladimir G. Karpenko ◽

...

Keyword(s):

Cord Blood ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Human Cord Blood ◽

Growth Properties ◽

Molecular Fraction

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Enhancement of erythrocyte oxygen transport function after hydrothermic storage under the action of human cord blood low-molecular fraction and the Actovegin drug

Reports of the National Academy of Sciences of Ukraine ◽

10.15407/dopovidi2020.11.085 ◽

2020 ◽

pp. 85-91

Author(s):

O.K. Gulevskyy ◽

◽

Yu.S. Akhatova ◽

Ye.Ye. Zharkova ◽

◽

...

Keyword(s):

Oxygen Saturation ◽

Cord Blood ◽

Oxygen Transport ◽

Relative Content ◽

Transport Function ◽

Human Cord Blood ◽

Long Term Storage ◽

Molecular Fraction ◽

Term Storage

We study the effect of a low-molecular fraction (below 10 kDa) derived from human cord blood and Actovegin on the content of various forms of hemoglobin in erythrocytes stored under hypothermia (2-4 ºС) for 21 days. During the long-term storage of erythrocytes, there is known to be a change in the hemoglobin forms ratio toward the decrease of the relative content of oxyhemoglobin and an increase of deoxy- and methemoglobin. As a result, the affinity of hemoglobin for oxygen reduces. The incubation of erythrocytes in a rehabilitation medium with each of the studied low-molecular drugs was found to be crucial to increase the content of oxyhemoglobin and to reduce the amounts of deoxy- and methemoglobin relative to the control. As a result, there was a rise in the oxygenation coefficient, reflecting the oxygen saturation of hemoglobin, up to the level characteristic of freshly isolated erythrocytes. The data obtained show that the human cord blood low-molecular fraction and the Actovegin normalize the ratio of hemoglobin forms in hypothermically stored erythrocytes, but the use of the Actovegin drug is more effective.

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