scholarly journals Detection and Investigation of Some Properties of the Regulators of Antibiotic Biosynthesis Produced by Streptomyces Strains S. sp. AN26 and S. sp. B35

2021 ◽  
Vol 83 (6) ◽  
pp. 49-54
Author(s):  
B.P. Matselyukh ◽  
Keyword(s):  
Molecular Structure ◽  
Nmr Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Transcriptional Regulators ◽  
Signaling Molecules ◽  
Molecular Structures ◽  
Antibiotic Biosynthesis ◽  
Future Studies ◽  
Layer Chromatography

The aim of this work was the isolation, purification and some properties investigation of two regulators of antibiotic biosynthesis of streptomycetes. Methods includes extraction of regulators from agar cultures and their concentration by vacuum rotary evaporator, thin layer chromatography and spectrophotometry. Results. Two strains of streptomycetes AN26 and B35 isolated from soils of different regions of Ukraine produce the regulators restoring the landomycin E biosynthesis and sporulation in mutant strain Streptomyces globispoprus 1912-B2. Both regulators were purified by thin layer chromatography and have the same Rf 0.69. Absorption curves of regulators were established by means of spectrophotometry. Maxima of absorption of regulators were 232.5 nm. The next study of the isolated regulators by means of NMR will give the possibility to elucidate their molecular structures. Conclusions. It is shown that two strains of streptomycetes isolated from the soils of Askania Nova and Brovary produce transcriptional regulators such as signaling molecules, which, like A-factor, restore the biosynthesis of antibiotics landomycin E and streptomycin in test strains S. globisporus 1912-B2 and S. griseis 1439, respectively. In terms of absorption maxima, they are similar and differ from similar indicators of known regulators of streptomycetes. It is possible that these compounds belong to new, not yet described signaling molecules, and the answer to this question will give future studies of their molecular structure by NMR spectroscopy.

scholarly journals Methods for the separation and identification of mycobactins from various species of mycobacteria

1968 ◽  
Vol 108 (4) ◽  
pp. 593-597 ◽  
Author(s):  
A. J. White ◽  
G. A. Snow
Keyword(s):  
Molecular Structure ◽  
Mass Spectrometry ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pure Component ◽  
Countercurrent Distribution ◽  
Side Chain ◽  
Layer Chromatography

A single pure component was isolated from mycobactin P by countercurrent distribution; its side chain is n-cis-octadec-2-enoyl; its purity and molecular structure were confirmed by mass spectrometry of its aluminium complex. The separation of ferric and of aluminium complexes of mycobactins by thin-layer chromatography is described. Mycobacterium terrae, M. marinum and M. smegmatis produce mycobactins that differ among themselves and from mycobactins P and T. A nomenclature for the mycobactins and their derivatives is suggested.

Changes in molecular structure of chickpea starch during processing treatments: A thin layer chromatography study

2018 ◽  
Vol 243 ◽  
pp. 186-191 ◽  
Author(s):  
Yongkang Sun ◽  
Hualei Wang ◽  
Wei Wang ◽  
Bing Hu ◽  
Li Zhou ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

scholarly journals Isolation and Structure Elucidation of Quercetin like Structure from Dalbergia sissoo (Fabaceae)

2020 ◽  
Vol 10 (3-s) ◽  
pp. 6-11
Author(s):  
Rohit Kumar KUMAR Bijauliya ◽  
Pushpendra Kannojia ◽  
Pankaj Mishra ◽  
Gaurav Kumar Pathak
Keyword(s):  
Nmr Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Column Chromatography ◽  
Structure Elucidation ◽  
High Performance ◽  
18Th Century ◽  
Dalbergia Sissoo ◽  
Layer Chromatography

The present study was conducted to isolate and classify Dalbergia sissoo (L.) bioactive compounds. The genus consists of 300 species in India, including 25 species. The generic name Dalbergia honors the 18th century Swedish brothers Nils and Carl Dalberg. Various phytoconstituents of alkaloids, glycosides, flavanoids, tannins, saponins, sterols and terpenoids were isolated and classified from different parts of the plant. Thin Layer Chromatography, High Performance Thin Layer Chromatography, and Column Chromatography were used to isolate spots from the fraction of the crude extract to elucidate the chemical structure of Dalbergia sissoo (L.) leaf extract. Potential spots have been exposed to techniques of FTIR, NMR and mass spectroscopy. Column chromatography was exposed to the raw extract, obtaining 125 fractions, conducting TLC. Among them was a single band in TLC, characterized by FTIR, NMR spectroscopy and mass spectroscopy, and the structure was known as Quercetin. The results of this study indicate the effective potential compound of the ethanolic fraction of Dalbergia sissoo (L.). Keywords: Dalbergia sissoo; Isolation & Structure Elucidation; FTIR; NMR spectroscopy; Mass spectroscopy.

α-Aminoacyl derivatives of α,ω-diaminopoly(oxyethylene)

1987 ◽  
Vol 52 (8) ◽  
pp. 1922-1927
Author(s):  
Bohumil Masař ◽  
Pavel Schmidt ◽  
Hana Pivcová ◽  
Pavel Čefelín
Keyword(s):  
Amino Acids ◽  
Nmr Spectroscopy ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Trifluoroacetic Acid ◽  
Complete Removal ◽  
Protective Group ◽  
H Nmr ◽  
Layer Chromatography ◽  
Derivatives Of

By reacting p-nitrophenyl esters of L-α-amino acids with α-(3-aminopropyl)-ω-(aminomethyl)-poly(oxyethylene), diamides having the structure [X-NHCH(R)CONH]2Mx' were prepared, X being the protective group Boc or Z, R being residues of glutamic and aspartic acid, phenylalanine and tyrosine, and Mx' being the poly(oxyethylene) chain with the (-CH2)3- and -CH2- endgroups. The diamides were characterized by IR and 1H NMR spectroscopy and thin-layer chromatography. After complete removal of tert-butyloxycarbonyl groups from diamides (X = Boc), deprotected α-aminoacyl derivatives were characterized as trifluoroacetic acid salts.

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