scholarly journals DNA loop domain rearrangements in blast transformed human lymphocytes and lymphoid leukaemic Jurkat T cells

2020 ◽  
Vol 92 (5) ◽  
pp. 62-69
Author(s):  
K. Afanasieva ◽  
◽  
V. Olefirenko ◽  
A. Martyniak ◽  
L. Lukash ◽  
...  
Keyword(s):  
T Cells ◽  
Human Lymphocytes ◽  
Jurkat T Cells ◽  
Loop Domain ◽  
Dna Loop

scholarly journals Human T-Lymphotropic Virus Type 1 Mitochondrion-Localizing Protein p13II Sensitizes Jurkat T Cells to Ras-Mediated Apoptosis

2005 ◽  
Vol 79 (15) ◽  
pp. 9449-9457 ◽  
Author(s):  
Hajime Hiraragi ◽  
Bindhu Michael ◽  
Amrithraj Nair ◽  
Micol Silic-Benussi ◽  
Vincenzo Ciminale ◽  
...  
Keyword(s):  
T Cells ◽  
Virus Type ◽  
Human Lymphocytes ◽  
Expression Vectors ◽  
Jurkat T Cells ◽  
Reading Frame ◽  
T Lymphotropic Virus ◽  
Adult T Cell Leukemia ◽  
Induced Apoptosis

ABSTRACT Human T-lymphotropic virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia. In addition to typical retroviral structural and enzymatic gene products, HTLV-1 encodes unique regulatory and accessory proteins, including a singly spliced pX open reading frame II (ORF II) product, p13II. We have demonstrated that proviral clones of HTLV-1 which are mutated in pX ORF II fail to obtain typical proviral loads and antibody responses in a rabbit animal model. p13II localizes to mitochondria and reduces cell growth and tumorigenicity in mice, but its function in human lymphocytes remains undetermined. For this study, we analyzed the functional properties of Jurkat T cells expressing p13II, using both transient and stable expression vectors. Our data indicate that p13II-expressing Jurkat T cells are sensitive to caspase-dependent, ceramide- and FasL-induced apoptosis. p13II-expressing Jurkat T cells also exhibited reduced proliferation when cultured at a high density. Furthermore, preincubation of the p13II-expressing cells with a farnesyl transferase inhibitor, which blocks the posttranslational modification of Ras, markedly reduced FasL-induced apoptosis, indicating the participation of the Ras pathway in p13II's influence on lymphocyte survival. Our data are the first to demonstrate that p13II alters Ras-mediated apoptosis in T lymphocytes, and they reveal a potential mechanism by which HTLV-1 alters lymphocyte proliferation.

A voltage-operable current is involved in Ca2+ entry in human lymphocytes whereas ICRAC has no apparent role

1996 ◽  
Vol 271 (5) ◽  
pp. C1494-C1503 ◽  
Author(s):  
J. J. Densmore ◽  
D. M. Haverstick ◽  
G. Szabo ◽  
L. S. Gray
Keyword(s):  
T Cells ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Nordihydroguaiaretic Acid ◽  
Jurkat Cells ◽  
Jurkat T Cells ◽  
Peripheral Blood Mononuclear ◽  
Voltage Gated

Presently, it is thought that a non-voltage-gated current is responsible for activation-induced Ca2+ entry in nonelectrically excitable cells such as lymphocytes. However, it has also been proposed that the pathway instead involves a second messenger-regulated Ca2+ channel that is voltage operable, where "voltage operable" is defined as an intrinsic property of the channel protein(s) rather than a requirement of normal gating. To evaluate the contribution of these currents to activation-induced Ca2+ influx, each was examined with respect to its ability to account for Ca2+ influx as reported by Ca(2+)-sensitive dyes. We identified a set of reagents, nordihydroguaiaretic acid and various calmodulin inhibitors, that inhibits Ca2+ entry and blocks the voltage-operable current but leaves the non-voltage-gated current unaltered. Further-more, nordihydroguaiaretic acid inhibited Ca(2+)-dependent proliferation of mitogen-activated human peripheral blood mononuclear cells or Jurkat T cells and specifically blocked Ca(2+)-dependent interleukin 2 production by Jurkat T cells to a degree similar to the immunosuppressant drug cyclosporin A. We also identified compounds, amiloride and Mn2+, that block the non-voltage-gated current but have no effect on either the voltage-operable current or Ca2+ entry. Correspondingly, amiloride had no effect on Ca(2+)-dependent proliferation of Jurkat cells. These observations imply that blockade of the non-voltage-gated current does not block either Ca2+ entry or Ca(2+)-dependent lymphocyte proliferation, whereas blockade of the voltage-operable current does. The data suggest that the voltage-operable current may be a mediator of activation-induced Ca2+ entry in lymphocytes.

scholarly journals Changes in loop organization of chromatin at different stages of lymphocyte activation

1970 ◽  
pp. 53-57
Author(s):  
K. S. Afanasieva ◽  
O. V. Lozovik ◽  
V. V. Olefirenko ◽  
A. V. Sivolob
Keyword(s):  
Comet Assay ◽  
Functional State ◽  
Large Scale ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Lymphocyte Activation ◽  
Domain Organization ◽  
Loop Domain ◽  
Dna Loop ◽  
Loop Migration

Aim. Aim was to investigate possible changes in the DNA loop domain organization upon activation of human lymphocytes. The rational for this task is the knowledge that the chromatin looping plays an important role in transcription regulation and thus may vary depending on cell functional state. Methods. The kinetics of DNA loop migration during single cell gel electrophoresis (the comet assay) was studied for nucleoids obtained from human lymphocytes and lymphoblasts activated to proliferation by interleukin 2. Results. Three part of DNA were observed in nucleoids: DNA on the nucleoid surface, loops up to ~150 kb inside the nucleoid, and larger loops that cannot migrate. An essential redistribution of the loop domains between the inside and surface fractions occurs upon activation (at G1 phase). Later on (at the end of S phase) the inside fraction becomes lower in favor of the large loops. Conclusions. Changes in the cell functional state are accompanied by large-scale changes in the loop domain organization that can be detected by the comet assay.Keywords: DNA loops, nucleoid, comet assay, lymphocytes, lymphoblasts.

The role of cbl family of ubiquitin ligases in gastric cancer exosome-induced apoptosis of Jurkat T cells

2009 ◽  
pp. 1-8
Author(s):  
Jing-Lei Qu ◽  
Xiu-Juan Qu ◽  
Ming-Fang Zhao ◽  
Yue-E Teng ◽  
Ye Zhang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
T Cells ◽  
Ubiquitin Ligases ◽  
Jurkat T Cells ◽  
Induced Apoptosis

scholarly journals Amorphous–Crystalline Calcium Phosphate Coating Promotes In Vitro Growth of Tumor-Derived Jurkat T Cells Activated by Anti-CD2/CD3/CD28 Antibodies

Materials ◽  
2021 ◽  
Vol 14 (13) ◽  
pp. 3693
Author(s):  
Yurii P. Sharkeev ◽  
Ekaterina G. Komarova ◽  
Valentina V. Chebodaeva ◽  
Mariya B. Sedelnikova ◽  
Aleksandr M. Zakharenko ◽  
...  
Keyword(s):  
T Cells ◽  
Calcium Phosphate ◽  
Biological Effects ◽  
Electrical Potential ◽  
Biological Properties ◽  
Modern Trend ◽  
Calcium Phosphate Coating ◽  
Jurkat T Cells ◽  
Micro Arc Oxidation

A modern trend in traumatology, orthopedics, and implantology is the development of materials and coatings with an amorphous–crystalline structure that exhibits excellent biocopatibility. The structure and physico–chemical and biological properties of calcium phosphate (CaP) coatings deposited on Ti plates using the micro-arc oxidation (MAO) method under different voltages (200, 250, and 300 V) were studied. Amorphous, nanocrystalline, and microcrystalline statesof CaHPO4 and β-Ca2P2O7were observed in the coatings using TEM and XRD. The increase in MAO voltage resulted in augmentation of the surface roughness Ra from 2.5 to 6.5 µm, mass from 10 to 25 mg, thickness from 50 to 105 µm, and Ca/P ratio from 0.3 to 0.6. The electrical potential (EP) of the CaP coatings changed from −456 to −535 mV, while the zeta potential (ZP) decreased from −53 to −40 mV following an increase in the values of the MAO voltage. Numerous correlations of physical and chemical indices of CaP coatings were estimated. A decrease in the ZP magnitudes of CaP coatings deposited at 200–250 V was strongly associated with elevated hTERT expression in tumor-derived Jurkat T cells preliminarily activated with anti-CD2/CD3/CD28 antibodies and then contacted in vitro with CaP-coated samples for 14 days. In turn, in vitro survival of CD4+ subsets was enhanced, with proinflammatory cytokine secretion of activated Jurkat T cells. Thus, the applied MAO voltage allowed the regulation of the physicochemical properties of amorphous–crystalline CaP-coatings on Ti substrates to a certain extent. This method may be used as a technological mechanism to trigger the behavior of cells through contact with micro-arc CaP coatings. The possible role of negative ZP and Ca2+ as effectors of the biological effects of amorphous–crystalline CaP coatings is discussed. Micro-arc CaP coatings should be carefully tested to determine their suitability for use in patients with chronic lymphoid malignancies.

scholarly journals Semi-purified extracts of Commelina benghalensis (Commelinaceae) induce apoptosis and cell cycle arrest in Jurkat-T cells

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Kgomotso Welheminah Lebogo ◽  
Matlou Phineas Mokgotho ◽  
Victor Patrick Bagla ◽  
Thabe Moses Matsebatlela ◽  
Vusi Mbazima ◽  
...  
Keyword(s):  
Cell Cycle ◽  
T Cells ◽  
Cell Cycle Arrest ◽  
Jurkat T Cells ◽  
Commelina Benghalensis ◽  
Cycle Arrest
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